Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,525,931 C→T R17H (CGC→CAC)  ansP ← L‑asparagine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,525,9310CT98.3% 229.4 / NA 60R17H (CGC→CAC) ansPL‑asparagine transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (27/32);  minor base G (1/0);  total (28/32)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAACAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGG  >  NC_000913/1525783‑1526078
                                                                                                                                                    |                                                                                                                                                   
aaCCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCATCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAgtgg                                                                                                                                                   >  2:36375/1‑151 (MQ=255)
aaCCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAgtgg                                                                                                                                                   >  2:658123/1‑151 (MQ=255)
      tgccagtgcGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTc                                                                                                                                             >  1:117389/1‑151 (MQ=255)
          agtgcGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCgcgg                                                                                                                                         >  2:975998/1‑151 (MQ=255)
           gtgcGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCgcggc                                                                                                                                        >  1:411064/1‑151 (MQ=255)
               gggCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCgtgt                                                                                                                                    <  2:397969/151‑1 (MQ=255)
                   cccGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGAt                                                                                                                                <  1:521705/151‑1 (MQ=255)
                             gCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAgtgtc                                                                                                                      <  1:977362/151‑1 (MQ=255)
                             gCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAgtgt                                                                                                                       <  2:594559/150‑1 (MQ=255)
                                 tCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAgtgtcggtg                                                                                                                  >  1:768582/1‑151 (MQ=255)
                                        ccTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCgtg                                                                                                             <  1:946481/149‑1 (MQ=255)
                                            cACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGCCGTGTTTACt                                                                                                       <  1:83062/151‑1 (MQ=255)
                                              ccTAAAAACAAGCCGCTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGTCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCgtg                                                                                                             <  2:1051471/143‑1 (MQ=255)
                                                 aaaaaCAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCAtt                                                                                                   <  1:959839/150‑1 (MQ=255)
                                                    aaCAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGctc                                                                                               <  1:898739/151‑1 (MQ=255)
                                                     aCAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGctct                                                                                              >  2:415591/1‑151 (MQ=255)
                                                            ggTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGAt                                                                                       <  2:655939/151‑1 (MQ=255)
                                                            ggTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGAt                                                                                       <  1:729793/151‑1 (MQ=255)
                                                             gTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGAt                                                                                       <  1:720381/150‑1 (MQ=255)
                                                              tGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTg                                                                                     >  1:323918/1‑151 (MQ=255)
                                                                 cAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCtt                                                                                  <  1:803300/151‑1 (MQ=255)
                                                                   aTCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTa                                                                                <  1:333822/151‑1 (MQ=255)
                                                                     cgcgCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAAt                                                                              >  2:429510/1‑151 (MQ=255)
                                                                       cgccgcCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGa                                                                            <  1:811834/151‑1 (MQ=255)
                                                                          cgccAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGaaa                                                                          >  1:129363/1‑150 (MQ=255)
                                                                           gccAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGGGGCGTTTCGCGGCGTGTTGATCTGAAGAGTCGGTGTCGTGATTACTCATTGCTCTCCCTGATTGCTTTAAAGaaaa                                                                         >  2:140052/1‑150 (MQ=255)
                                                                             cAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAg                                                                       >  2:608169/1‑150 (MQ=255)
                                                                             cAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGt                                                                      >  2:992338/1‑151 (MQ=255)
                                                                              aaTGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTc                                                                     <  2:187046/151‑1 (MQ=255)
                                                                                tGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCat                                                                   >  2:989909/1‑151 (MQ=255)
                                                                                   cGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCatat                                                                 >  1:352256/1‑150 (MQ=255)
                                                                                   cGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCAtata                                                                <  2:523219/151‑1 (MQ=255)
                                                                                             cACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATg                                                      >  2:701339/1‑151 (MQ=255)
                                                                                             cACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATg                                                      <  1:659901/151‑1 (MQ=255)
                                                                                              aCCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATg                                                      >  2:256770/1‑150 (MQ=255)
                                                                                                  ggCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAACaa                                                 <  2:680575/151‑1 (MQ=255)
                                                                                                     gATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAACAATGt                                              <  1:489320/151‑1 (MQ=255)
                                                                                                     gATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAACAATGt                                              >  1:350334/1‑151 (MQ=255)
                                                                                                         gCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAACAATGTTTa                                           <  2:1016686/150‑1 (MQ=255)
                                                                                                            cATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAACAATGTTTATTc                                        >  1:563231/1‑150 (MQ=255)
                                                                                                            cATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAACAATGTTTATTCa                                       <  1:1062080/151‑1 (MQ=255)
                                                                                                            cATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAACAATGTTTATTCa                                       <  1:409899/151‑1 (MQ=255)
                                                                                                                gCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAACAATGTTTATTCATCCt                                   >  2:1018351/1‑151 (MQ=255)
                                                                                                                 cTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAACAATGTTTATTCATCCTg                                  >  1:171325/1‑151 (MQ=255)
                                                                                                                  tttGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAACAATGTTTATTCATCCTgg                                 >  1:868888/1‑151 (MQ=255)
                                                                                                                   ttGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGCAGTGGCGGTGTCGGGTTTACTCATTGCTCTCCCTGATTGCTTTTATGAAAAAGTCATATAAGTTGCCATGAACAATGTTTTTTCATCCt                                   >  2:938408/1‑148 (MQ=255)
                                                                                                                   ttGTGATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAACAATGTTTATTCATCCTg                                  <  1:819197/149‑1 (MQ=255)
                                                                                                                    tgtgATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAACAATGTTTATTCATCCTGGc                                <  2:371713/150‑1 (MQ=255)
                                                                                                                     gtgATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAACAATGTTTATTCATCCTGGCga                              <  2:940165/151‑1 (MQ=255)
                                                                                                                       gATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAACAATGTTTATTCATCCTGGCgatg                            <  1:224252/151‑1 (MQ=255)
                                                                                                                       gATACCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAACAATGTTTATTCATCCTGGCgatg                            >  1:348484/1‑151 (MQ=255)
                                                                                                                          aCCCCTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAACAATGTTTATTCATCCTGGCGATGATa                         <  1:778467/151‑1 (MQ=255)
                                                                                                                             ccTCTTCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAACAATGTTTATTCATCCTGGCGATGATAGtt                      >  2:952441/1‑151 (MQ=255)
                                                                                                                                  tCGTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAACAATGTTTATTCATCCTGGCGATGATAGTTAt                    <  1:520199/148‑1 (MQ=255)
                                                                                                                                    gTGGGCATTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAACAATGTTTATTCATCCTGGCGATGATAGTTATCAcc                >  2:952778/1‑150 (MQ=255)
                                                                                                                                          aTTAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAACAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTc         <  2:1013893/151‑1 (MQ=255)
                                                                                                                                           ttAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGGGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTATAAGGAAAAAGGCATTTAAGTTGCCATGAACAAAGTTTATTCATCCTGGCGATTGTAGTTATCACCATTATTCt        >  1:238164/1‑151 (MQ=255)
                                                                                                                                            tAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAACAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCtt       >  2:195020/1‑151 (MQ=255)
                                                                                                                                            tAAGCCAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAACAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCt        <  1:658157/150‑1 (MQ=255)
                                                                                                                                                ccAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAACAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATg   <  1:876160/151‑1 (MQ=255)
                                                                                                                                                 cAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAACAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATgg  >  2:776546/1‑151 (MQ=255)
                                                                                                                                                 cAGTGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAACAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATgg  >  2:113431/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCTGAAGTGTCGGTGTCGTGTTTACTCATTGCTCTCCCTGATTGCTTTAATGAAAAAGTCATATAAGTTGCCATGAACAATGTTTATTCATCCTGGCGATGATAGTTATCACCATTATTCTTTATGG  >  NC_000913/1525783‑1526078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: