Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,560,722	A→G	Y70H (TAC→CAC)	ddpB ←	D,D‑dipeptide ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,560,722	0	A	G	100.0%	104.4 / NA	33	Y70H (TAC→CAC)	ddpB	D,D‑dipeptide ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/22);  total (11/22)

CCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAG  >  NC_000913/1560575‑1560867                                                                                                                                                    |                                                                                                                                                   ccAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCg                                                                                                                                                 <  2:835119/151‑1 (MQ=255)           gCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTg                                                                                                                                       >  2:172523/1‑150 (MQ=255)               aTCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGtaca                                                                                                                                   >  1:579744/1‑150 (MQ=255)                      gTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTgg                                                                                                                           <  1:634451/151‑1 (MQ=255)                        gCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCt                                                                                                                         <  2:574404/151‑1 (MQ=255)                        gCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCt                                                                                                                         <  2:347795/151‑1 (MQ=255)                                    gCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAGTCCCa                                                                                                             <  2:452101/151‑1 (MQ=255)                                    gCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAGTCCCa                                                                                                             <  1:734926/151‑1 (MQ=255)                                       ccAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCCCCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAGTCCCAGtt                                                                                                          <  1:870973/151‑1 (MQ=255)                                              ccAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCg                                                                                                   >  1:499401/1‑151 (MQ=255)                                              ccAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCg                                                                                                   <  1:725725/151‑1 (MQ=255)                                                  cTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGGCCCGGCCCCGTTGCTGGCGaa                                                                                                 >  2:104678/1‑149 (MQ=255)                                                      aGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCCGGGCCCGGCCCCGTTTCTTGGGGAAAtttt                                                                                           >  2:48690/1‑151 (MQ=255)                                                         gTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCa                                                                                        <  1:316096/151‑1 (MQ=255)                                                             ccGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGAt                                                                                    <  2:824573/151‑1 (MQ=255)                                                                ggAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTTCACGATAg                                                                                  >  1:818414/1‑150 (MQ=255)                                                                ggAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGc                                                                                 <  1:595290/151‑1 (MQ=255)                                                                  aaaaaTATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCAt                                                                               <  2:975879/151‑1 (MQ=255)                                                                          aCGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGgc                                                                        <  1:745760/150‑1 (MQ=255)                                                                           cGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCCGGCGAATATTTTCCACGATAGCATCGCTGgcgc                                                                      <  2:420896/151‑1 (MQ=255)                                                                             cAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCgg                                                                    <  1:754872/151‑1 (MQ=255)                                                                                         cACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCCATCCCCCGTGCCGGCGCAAATTTTCCACAACAGCCTTGCTTGCGCCGTCAcccccc                                                           >  2:107082/1‑148 (MQ=255)                                                                                                   ccGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGGCCAGGCCCCGGTGCTGGCGGATATTTTTCACCAAAACATCGCCGGCGCGGGCCCCCGCCCGTAACCCCGcc                                               >  2:391418/1‑150 (MQ=255)                                                                                                       tccGAATGGATTTCCCCAGGTCCCCAGGAACCGGGTCTGTGA‑GGGGGGGTAAAACCTGTAAATACAGTGGCTGGTCAAGTCCCAGTTCCGGCCAAATATTTTCCAGTAATGCTTGCCTGGGGCGGCCTCCCGCCAGTAACCGCGCCg                                              <  2:514122/145‑1 (MQ=255)                                                                                                                 tGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCGGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCCCCCGCCAGTAACCGCGCCGGATCACCAGGGAt                                 <  1:324031/150‑1 (MQ=255)                                                                                                                   ttCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGGGGCTGGTCCCATGCCAATTTCTTGCGAATATTTTCCCAGAAAACATCGCCGGCCCCGACACCCCCCAGAAAACCCCCCGCATCACCCggg                                   >  2:422208/1‑146 (MQ=255)                                                                                                                    tCCCAGGTCACCATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAATCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTTGCCCGGTCACCCGCCCGTAACCGCGCCGGATCACCCGGGATc                                >  1:909005/1‑148 (MQ=255)                                                                                                                      caaGGACACCATGAAACAGGTCGCGGACGTGGGGGAAAAA‑CTGTACATACAGGGGCTGGCCCAGCCCCAGTTGCTGCCGAATATTTTCCACGATAGCACCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCa                               <  2:469346/145‑1 (MQ=255)                                                                                                                            cACCATGAAACGGGTCTCTGTCGGGGCTGTAAAA‑TTGTACAGACGGTGGCGGGTCCATTCCCATTTCCTGACGATTTTTTTCCACAATACCAACGTGGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCgaga                      <  2:632779/150‑1 (MQ=255)                                                                                                                               cATGAAACAGGTCGCTGACGTGGCGGTAAAA‑CTGCACATACAGTGGCTGGGCCACACCCCGTTGCTGGGGAAAATTTTCCACGAAAGGAACGCCGGGGCGGTGAACCCCCCGAAAACCCGCCCGATCCCCAGGGacacaaaacgagaga                    >  1:100655/1‑135 (MQ=255)                                                                                                                                     acgggTCGCTGACGTGGCGGAAAAA‑TTGTCCATACAGTGGCTGGTCCAGTCCCAGTTGCGGGCGAATATTTTCCACGATACCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGt            <  1:520424/148‑1 (MQ=255)                                                                                                                                        ggTCGCTGACGTGGCGGTAAAC‑CTGTACATACAGGGGCTGGCCCAGCCCCAGTTGCTGGCGAATATTTCCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAAt         <  1:905995/151‑1 (MQ=255)                                                                                                                                               gACGTGGCGGAAAAC‑CTGTACAAACAGTGGCTGGCCCAGCCCCAGTTGCTGGCGAATATTTCCCGCGATAGCATCGCTGGGGCGGTCAACCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAg  <  2:439411/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   CCAGCGGGATGCCAATCAGGAGTGCCAGCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAA‑CTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAG  >  NC_000913/1560575‑1560867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑