Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,607,481 A→G T46A (ACC→GCC)  tam → trans‑aconitate methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,607,4810AG98.6% 223.6 / NA 71T46A (ACC→GCC) tamtrans‑aconitate methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (39/31);  total (40/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

CGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAACGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCACCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGCCAGTTCGTGGAAGCAGACATCCGCAACTGGCAACCGGTACAGGCACTCG  >  NC_000913/1607333‑1607628
                                                                                                                                                    |                                                                                                                                                   
cGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAACGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGcc                                                                                                                                                   >  1:314690/1‑151 (MQ=255)
cGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAACGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGcc                                                                                                                                                   <  2:736695/151‑1 (MQ=255)
      tAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAACGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCtt                                                                                                                                             >  2:652511/1‑151 (MQ=255)
         aaaaaTGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAACGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCt                                                                                                                                           >  1:331925/1‑150 (MQ=255)
          aaaaTGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAACGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTac                                                                                                                                         >  1:19819/1‑151 (MQ=255)
           aaaTGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAACGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTaca                                                                                                                                        <  2:645849/151‑1 (MQ=255)
            aaTGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAACGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTaca                                                                                                                                        >  2:766603/1‑150 (MQ=255)
             aTGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAACGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTacaa                                                                                                                                       >  2:324557/1‑150 (MQ=255)
                 cTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAACGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACg                                                                                                                                  >  2:471652/1‑151 (MQ=255)
                   gACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAACGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGtt                                                                                                                                <  1:918776/151‑1 (MQ=255)
                     cTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAACGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTgg                                                                                                                              >  2:222266/1‑151 (MQ=255)
                       ggAACCCCTCTTTATATCTACACTTTTCCGCTGAACGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGcc                                                                                                                            <  1:153510/151‑1 (MQ=255)
                         aaCCCCTCTTTATATCTACACTTTTCCGCTGAACGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTg                                                                                                                          <  1:798694/151‑1 (MQ=255)
                            cccTCTTTATATCTACACTTTTCCGCTGAACGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCgg                                                                                                                       <  1:331091/151‑1 (MQ=255)
                            cccTCTTTATATCTACACTTTTCCGCTGAACGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCgg                                                                                                                       >  2:518287/1‑151 (MQ=255)
                                 tttATATCTACACTTTTCCGCTGAACGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGTAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGCAACAGCACCGCCCTTTTACAACAACGTTGGCCGGCGGCCCgg                                                                                                                  >  2:457165/1‑151 (MQ=255)
                                  ttATATCTACACTTTTCCGCTGAACGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAg                                                                                                                   <  1:284059/149‑1 (MQ=255)
                                   tataTCTACACTTTTCCGCTGAACGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGAt                                                                                                                >  1:191830/1‑151 (MQ=255)
                                    atatCTACACTTTTCCGCTGAACGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATa                                                                                                               <  1:128296/151‑1 (MQ=255)
                                         tACACTTTTCCGCTGAACGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAgg                                                                                                          >  2:113927/1‑151 (MQ=255)
                                               tttCCGCTGAACGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCg                                                                                                     >  2:761155/1‑150 (MQ=255)
                                               tttCCGCTGAACGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCg                                                                                                     <  1:959700/150‑1 (MQ=255)
                                                ttCCGCTGAACGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGa                                                                                                    <  1:789294/150‑1 (MQ=255)
                                                ttCCGCTGAACGATAGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGGATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACCACAACGTTGGCCTGCGGCCAGGATAAAAGGCATCg                                                                                                     >  2:436432/1‑149 (MQ=255)
                                                  ccGCTGAACGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTc                                                                                                 >  2:230618/1‑151 (MQ=255)
                                                      tGAACGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGtct                                                                                             >  2:542338/1‑151 (MQ=255)
                                                      tGAACGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGtct                                                                                             >  1:934914/1‑151 (MQ=255)
                                                      tGAACGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGtct                                                                                             >  2:275177/1‑151 (MQ=255)
                                                      tGAACGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGtc                                                                                              >  2:869639/1‑150 (MQ=255)
                                                        aaCGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGTATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTcc                                                                                           >  1:762164/1‑151 (MQ=255)
                                                              cgcgTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCgat                                                                                     <  2:546488/151‑1 (MQ=255)
                                                               gcgTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCgatg                                                                                    >  1:853594/1‑151 (MQ=255)
                                                                     ggcggTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACACGTCTCCGGCGATGATTGCt                                                                              <  1:984430/151‑1 (MQ=255)
                                                                                    tGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCt                                                               >  1:20208/1‑151 (MQ=255)
                                                                                    tGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCATCTCCGGCGATGATTGCTGAAGCGCGCAGTg                                                                 <  1:641962/149‑1 (MQ=255)
                                                                                     gCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGACCTTCTACAACAGCGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCtt                                                              <  2:331304/151‑1 (MQ=255)
                                                                                        agagTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGc                                                           >  1:387785/1‑151 (MQ=255)
                                                                                          agTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGcc                                                          >  2:881488/1‑150 (MQ=255)
                                                                                          agTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGcc                                                          >  2:972111/1‑150 (MQ=255)
                                                                                            tGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGcc                                                          >  1:247053/1‑148 (MQ=255)
                                                                                                  tGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGcc                                                  >  1:215795/1‑150 (MQ=255)
                                                                                                  tGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGcc                                                  >  1:935878/1‑150 (MQ=255)
                                                                                                   gtcaaaTGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGcc                                                  >  2:141511/4‑149 (MQ=255)
                                                                                                    gAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGa                                                                            <  1:288058/122‑1 (MQ=255)
                                                                                                    gAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGa                                                                            >  2:288033/1‑122 (MQ=255)
                                                                                                    gAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGCCAGt                                               >  1:778114/1‑151 (MQ=255)
                                                                                                     aaaaTGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGCCAGtt                                              >  1:626074/1‑151 (MQ=255)
                                                                                                     aaaaTGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGCCAGtt                                              <  1:934191/151‑1 (MQ=255)
                                                                                                        aTGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGCCAGTTCGt                                           <  1:574323/151‑1 (MQ=255)
                                                                                                            cGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGCCAGTTCGTGGaa                                       <  1:82509/151‑1 (MQ=255)
                                                                                                              aaTATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGCCAGTTCGTGGAAGc                                     >  1:220648/1‑151 (MQ=255)
                                                                                                               atatGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGCCAGTTCGTGGAAGCa                                    <  1:175322/151‑1 (MQ=255)
                                                                                                                 atGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGCCAGTTCGTGGAAGCAg                                   <  2:34255/150‑1 (MQ=255)
                                                                                                                 atGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGCCAGTTCGTGGAAGCAg                                   <  1:647479/150‑1 (MQ=255)
                                                                                                                      gCCGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGCCAGTTCGTGGAAGCAGACATc                              <  2:268987/150‑1 (MQ=255)
                                                                                                                       ccGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGCCAGTTCGTGGAAGCAGACATcc                             >  1:992988/1‑150 (MQ=255)
                                                                                                                       ccGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGCCAGTTCGTGGAAGCAGACATCCg                            >  1:44102/1‑151 (MQ=255)
                                                                                                                        cGATCTTGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGCCAGTTCGTGGAAGCAGACATCCGc                           >  2:354669/1‑151 (MQ=255)
                                                                                                                         gATATTGGCTGCGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGCCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGCCAGTTCGTGGAGGCGGACATCCGc                           >  2:188459/1‑150 (MQ=255)
                                                                                                                              tGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGCCAGTTCGTGGAAGCAGACATCCGc                           <  1:1013037/145‑1 (MQ=255)
                                                                                                                              tGGCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGCCAGTTCGTGGAAGCAGACATCCGCaa                         <  2:494517/147‑1 (MQ=255)
                                                                                                                               ggCTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGCCAGTTCGTGGAAGCAGACATCCGCAACTgg                     <  1:944388/150‑1 (MQ=255)
                                                                                                                                 cTGTGGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGCCAGTTCGTGGAAGCAGACATCCGCAACTGGCa                   >  1:737858/1‑150 (MQ=255)
                                                                                                                                   gtgGCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGCCAGTTCGTGGAAGCAGACATCCGCAACTGGCAAcc                <  2:277705/151‑1 (MQ=255)
                                                                                                                                     ggCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGCCAGTTCGTGGAAGCAGACATCCGCAACTGGCAACCgg              <  1:73511/151‑1 (MQ=255)
                                                                                                                                     ggCCCAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGCCAGTTCGTGGAAGCAGACATCCGCAACTGGCAACCg               <  2:1047252/150‑1 (MQ=255)
                                                                                                                                         cAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGCCAGTTCGTGGAAGCAGACATCCGCAACTGGCAACCGGTAc           >  2:108330/1‑150 (MQ=255)
                                                                                                                                         cAGGTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGCCAGTTCGTGGAAGCAGACATCCGCAACTGGCAACCGGTACa          <  2:957477/151‑1 (MQ=255)
                                                                                                                                            gTAACAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGCCAGTTCGTGGAAGCAGACATCCGCAACTGGCAACCGGTACAGGc       <  2:188924/151‑1 (MQ=255)
                                                                                                                                                cAGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGCCAGTTCGTGGAAGCAGACATCCGCAACTGGCAACCGGTACAGGCACTc   >  2:337411/1‑151 (MQ=255)
                                                                                                                                                 aGCGCCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGCCAGTTCGTGGAAGCAGACATCCGCAACTGGCAACCGGTACAGGCACTCg  <  1:971665/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGGGAGTAAAAAAATGTCTGACTGGAACCCCTCTTTATATCTACACTTTTCCGCTGAACGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGTGCCGCTGGAAAATGTCGAATATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCACCGCCCTTCTACAACAACGTTGGCCTGCGGCCAGGATAACAGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGCCAGTTCGTGGAAGCAGACATCCGCAACTGGCAACCGGTACAGGCACTCG  >  NC_000913/1607333‑1607628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: