Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,681,679	G→A	G235D (GGC→GAC)	folM →	dihydromonapterin reductase, NADPH‑dependent; dihydrofolate reductase isozyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,681,679	0	G	A	100.0%	324.8 / NA	85	G235D (GGC→GAC)	folM	dihydromonapterin reductase, NADPH‑dependent; dihydrofolate reductase isozyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/47);  total (38/47)

TTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAAT  >  NC_000913/1681532‑1681825                                                                                                                                                    |                                                                                                                                                   ttAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACgg                                                                                                                                                 >  1:58447/1‑151 (MQ=255)  tAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGgt                                                                                                                                                >  1:61137/1‑151 (MQ=255)      gAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGgtcgtc                                                                                                                                            <  1:235571/151‑1 (MQ=255)       aaCATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCa                                                                                                                                           <  2:31673/151‑1 (MQ=255)          atgatgatgCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGTCCTGGTCGATTACTTACTTTCCAGTTGCTTTGTCACCGTACGCAGTTTCCCACTTGATGACGGTCGTCATCt                                                                                                                                        <  1:335872/151‑1 (MQ=255)          atgatgatgCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCt                                                                                                                                        >  2:879337/1‑151 (MQ=255)               gatgCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGtt                                                                                                                                   >  1:492759/1‑151 (MQ=255)               gatgCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGt                                                                                                                                    <  1:342044/150‑1 (MQ=255)                atgCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGtt                                                                                                                                   >  2:332717/1‑150 (MQ=255)                atgCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTa                                                                                                                                  <  2:599746/151‑1 (MQ=255)                  gCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTaa                                                                                                                                 <  2:1021379/150‑1 (MQ=255)                  gCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTaa                                                                                                                                 <  2:149058/150‑1 (MQ=255)                  gCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAAt                                                                                                                                <  1:858675/151‑1 (MQ=255)                   ccGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATg                                                                                                                               <  2:164524/151‑1 (MQ=255)                    cGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGc                                                                                                                              <  1:973359/151‑1 (MQ=255)                    cGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGc                                                                                                                              <  2:940714/151‑1 (MQ=255)                     gAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAAt                                                                                                                                <  2:557438/148‑1 (MQ=255)                       atatCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGc                                                                                                                           >  1:47538/1‑151 (MQ=255)                          tCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCttt                                                                                                                        >  1:180283/1‑151 (MQ=255)                           cGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCttt                                                                                                                        <  1:1006973/150‑1 (MQ=255)                                  aggcaatGAATAAATCACTGATGAAAACCACGCCTGGCGAGAAAGAAGTGATCGACCTGGACGATTACTTACTTACGAGTTGCTGTGTAAACGGACGCAGTTTCCCACGTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCGAGc                                                                                                                 <  2:179639/144‑1 (MQ=255)                                     cgcTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCatat                                                                                                             >  1:977609/1‑151 (MQ=255)                                       cTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCAtata                                                                                                            >  1:484209/1‑150 (MQ=255)                                          aaTAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATc                                                                                                         >  1:672560/1‑150 (MQ=255)                                          aaTAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATc                                                                                                         >  1:282551/1‑150 (MQ=255)                                           aTAAATCACTAATGAAAAACGCGCCTGGCGAGAAAGAAGTGAGCGACCTGTTCGATTACTTACTTACCAGTTGTTTTATCACCGGCCGCAGTTTCCCACTTGAGGACGGTCGTCATCTGCGTTAATGCACCTTTATCCAgtagac                                                                                                             <  2:332268/145‑6 (MQ=255)                                             aaaTCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAac                                                                                                      >  1:201143/1‑150 (MQ=255)                                              aaTCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAacac                                                                                                    <  1:479303/151‑1 (MQ=255)                                                 cACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACAccc                                                                                                  >  2:246099/1‑150 (MQ=255)                                                 cACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCa                                                                                                 <  1:546053/151‑1 (MQ=255)                                                  aCTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCAt                                                                                                >  2:272919/1‑151 (MQ=255)                                                    tgatgaAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATgc                                                                                              >  1:873280/1‑151 (MQ=255)                                                     gatgaAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATgcg                                                                                             >  1:769440/1‑151 (MQ=255)                                                      atgaAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATgcgc                                                                                            <  1:763053/151‑1 (MQ=255)                                                        gaAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCtt                                                                                          <  1:290552/151‑1 (MQ=255)                                                         aaaaCCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGCCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGGATATAATCAACACCCATGCGCtt                                                                                          >  2:92308/1‑150 (MQ=255)                                                         aaaaCCGCGCATGGCGAGAACGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTACCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCtt                                                                                          >  2:486529/1‑150 (MQ=255)                                                              cgcgCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATcccc                                                                                    >  1:336353/1‑151 (MQ=255)                                                              cgcgCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATcccc                                                                                    <  2:734246/151‑1 (MQ=255)                                                                cgcCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATccccc                                                                                   >  2:976287/1‑150 (MQ=255)                                                                cgcCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCa                                                                                  <  2:990950/151‑1 (MQ=255)                                                                  ccTGGCGAGAAAGAAGTGATAGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAac                                                                                >  2:40914/1‑151 (MQ=255)                                                                        gagaAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCa                                                                           >  1:735719/1‑150 (MQ=255)                                                                            aagaagTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGa                                                                      <  2:649370/151‑1 (MQ=255)                                                                              gaagTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAAc                                                                    <  1:1040270/151‑1 (MQ=255)                                                                               aagTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAAcc                                                                   <  1:640173/151‑1 (MQ=255)                                                                                 gTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACca                                                                  >  1:393385/1‑150 (MQ=255)                                                                                      cGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACaaaa                                                            <  2:736611/151‑1 (MQ=255)                                                                                       gACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACaaa                                                             >  2:737440/1‑149 (MQ=255)                                                                                            ggTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAgcggcg                                                      <  2:808006/151‑1 (MQ=255)                                                                                               cGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCggg                                                    <  1:464654/150‑1 (MQ=255)                                                                                               cGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCggg                                                    <  1:142363/150‑1 (MQ=255)                                                                                                   tacttacttacCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAg                                                >  1:160912/1‑150 (MQ=255)                                                                                                   tacttacttacCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAg                                                <  1:676667/150‑1 (MQ=255)                                                                                                    acttacttacCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACGCCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAgct                                              <  2:146381/151‑2 (MQ=255)                                                                                                        acttacCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGcat                                           <  1:560182/150‑1 (MQ=255)                                                                                                        acttacCAGTTGCTTTGTCACCGGACGCAGTTGCTCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTTTCCAGCATATAACCACCCCCCATGCGCTTATCCCCCAACACGCAACAGATCCACCAAAAGCGGCTGGCAAGCGtat                                           <  2:46135/150‑1 (MQ=255)                                                                                                          ttacCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGcatcat                                        >  2:713850/1‑151 (MQ=255)                                                                                                           tacCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGcatca                                         <  1:836964/149‑1 (MQ=255)                                                                                                           tacCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCAtt                                       >  1:219988/1‑151 (MQ=255)                                                                                                            acCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTc                                      <  2:600317/151‑1 (MQ=255)                                                                                                            acCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTc                                      <  1:704651/151‑1 (MQ=255)                                                                                                               aGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGACAAGCGCATCATTCCgg                                   <  2:138606/151‑1 (MQ=255)                                                                                                                gTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGt                                  <  2:987063/151‑1 (MQ=255)                                                                                                                     tttGTCACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCCAGCGCATCATTCCGGTGaaa                              >  2:247015/1‑150 (MQ=255)                                                                                                                          cACCGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACcac                        <  2:154062/151‑1 (MQ=255)                                                                                                                            ccGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACcac                        >  1:465458/1‑149 (MQ=255)                                                                                                                            ccGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAg                      <  2:849951/151‑1 (MQ=255)                                                                                                                            ccGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAg                      <  1:976338/151‑1 (MQ=255)                                                                                                                             cGGACGCAGTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGc                     >  1:962447/1‑151 (MQ=255)                                                                                                                                     gTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACAcc               >  2:630908/1‑149 (MQ=255)                                                                                                                                     gTTTCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAg             <  2:771675/151‑1 (MQ=255)                                                                                                                                        tCCCACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGt           <  2:407668/150‑1 (MQ=255)                                                                                                                                         cccACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTaa         <  1:331212/151‑1 (MQ=255)                                                                                                                                          ccACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCCCAGCGACACCAGGTaaa        <  2:841429/151‑1 (MQ=255)                                                                                                                                          ccACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTaaa        <  1:486845/151‑1 (MQ=255)                                                                                                                                           cACTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTaaa        >  1:1003240/1‑150 (MQ=255)                                                                                                                                             cTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACa      >  1:23320/1‑150 (MQ=255)                                                                                                                                             cTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACa      >  2:1063236/1‑150 (MQ=255)                                                                                                                                             cTTGATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGAGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACa      >  2:1004707/1‑150 (MQ=255)                                                                                                                                              ttGATGACGGGCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCCAGGGCACCATTCCGGGGAAAAACCCCACCCAACCCAGGTAAACa      >  2:665239/1‑149 (MQ=255)                                                                                                                                               tgatgaCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACaaa    <  2:424946/150‑1 (MQ=255)                                                                                                                                                gATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAAt  <  1:572567/151‑1 (MQ=255)                                                                                                                                                gATGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCACAACACGCAACAGAACCAACAAACGCGGCGGGCAAGCGCATCATTCCGGTGAAATGCCACAGCGACACCAGGTAAACAAAAt  <  2:588614/151‑1 (MQ=255)                                                                                                                                                 aTGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCCTCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAAt  >  2:91400/1‑150 (MQ=255)                                                                                                                                                 aTGACGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAAt  >  2:273770/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                   TTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGTTAATGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAAGCGCATCATTCCGGTGAAATACCACAGCGACACCAGGTAAACAAAAT  >  NC_000913/1681532‑1681825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑