Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,789,056	T→C	F208L (TTC→CTC)	aroH →	3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tryptophan repressible

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,789,056	0	T	C	100.0%	171.4 / NA	47	F208L (TTC→CTC)	aroH	3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tryptophan repressible
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/29);  total (18/29)

GGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGTGGCGGCAAAAAACCGAATTATCATGCCGATGATATCGCCGCAGCCTGCGATACGCTGCACGAGTTTGA  >  NC_000913/1788908‑1789201                                                                                                                                                     |                                                                                                                                                  gggCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGCTc                                                                                                                                                 <  1:56682/151‑1 (MQ=255)   gcgcGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCGGCACTCTCCTGTCCGGTAGGTTTTAAACATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGCTCct                                                                                                                                               >  2:282242/1‑151 (MQ=255)       gATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCg                                                                                                                                           <  2:854552/151‑1 (MQ=255)         ttGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGcc                                                                                                                                         >  2:319208/1‑151 (MQ=255)            gcgcACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGa                                                                                                                                      <  1:126011/151‑1 (MQ=255)              gcACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGAc                                                                                                                                     >  2:629311/1‑150 (MQ=255)                 cGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACaaa                                                                                                                                  >  2:574710/1‑150 (MQ=255)                        ccGAAAGTCAGATCCACCGCGAAATGGCTTCGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCa                                                                                                                          <  2:505171/151‑1 (MQ=255)                                     ccACCGCGAAATGGCTTCGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTAt                                                                                                             <  2:93417/151‑1 (MQ=255)                                            gAAATGGCTTCGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCa                                                                                                      <  2:880290/151‑1 (MQ=255)                                                       ggCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAAccc                                                                                            <  2:685215/150‑1 (MQ=255)                                                          aCTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTa                                                                                         >  2:741019/1‑150 (MQ=255)                                                             ctcCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATg                                                                                       <  1:374091/149‑1 (MQ=255)                                                              tcCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGAAATACGCGGATTGCTGTGGATGCTATCCGAGCAGCACGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGACAGATGACCATCTATCAAACCAGCGGCAACCCGTATg                                                                                       <  2:1014025/148‑1 (MQ=255)                                                                  gTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACat                                                                                >  1:548983/1‑151 (MQ=255)                                                                  gTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACat                                                                                >  2:445663/1‑151 (MQ=255)                                                                    ccGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACatt                                                                               >  1:499179/1‑150 (MQ=255)                                                                     cGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACattat                                                                             >  2:512988/1‑151 (MQ=255)                                                                         aGGTTTTAAAAATGGTACCGATGCCAGTACGCGGATTGCTGTGGATGCTATGCGCGCAGCCCGCGCCAGCCATATGCTCGTATCACCAGACAAAAGTGGTCAGAGGACCAGCTATTAGACCAACGGCAAGCCGTAGGGTCACATTATTCTg                                                                         >  2:123583/1‑151 (MQ=255)                                                                                  aaaTGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGTggcggc                                                                >  2:554558/1‑151 (MQ=255)                                                                                  aaaTGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGTggcggc                                                                <  2:168676/151‑1 (MQ=255)                                                                                   aaTGGTACCGATGGCAATACGCGGATTGCTGTGGATGTTATCCGCGCAGCCCACGCCAGCCATATGCTCCTGTCGCCAGACAAAAATGATCAGATGACCATCTATCAGACGAGCGGCAACCCGTATGGCCACATTATTATGCGTggcga                                                                 <  2:968572/149‑2 (MQ=255)                                                                                   aaTGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGTggcggc                                                                >  2:530141/1‑150 (MQ=255)                                                                                   aaTGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGTGGCGGCa                                                               <  2:414958/151‑1 (MQ=255)                                                                                      ggTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGTGGCGGCaaaa                                                            <  1:984819/151‑1 (MQ=255)                                                                                                     aCGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGTGGCGGCAAAAAACCGAATTATCAt                                              <  1:177408/150‑1 (MQ=255)                                                                                                         ggATTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGTGGCGGCAAAAAACCGAATTATCATGCCga                                         <  1:166608/151‑1 (MQ=255)                                                                                                         ggATTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGTGGCGGCAAAAAACCGAATTATCATGCCga                                         <  2:1004828/151‑1 (MQ=255)                                                                                                          gATTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGTGGCGGCAAAAAACCGAATTATCATGCCgat                                        >  2:552375/1‑151 (MQ=255)                                                                                                           aTTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGTGGCGGCAAAAAACCGAATTATCATGCCgatg                                       <  2:631926/151‑1 (MQ=255)                                                                                                             tGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGTGGCGGCAAAAAACCGAATTATCATGCCgatgat                                     >  1:81668/1‑151 (MQ=255)                                                                                                                 gtgGATGCTATCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGTGGCGGCAAAAAACCGAATTATCATGCCGATGATATcg                                 <  2:844556/151‑1 (MQ=255)                                                                                                                 gtgGATGCTATCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGTGGCGGCAAAAAACCGAATTATCATGCCGATGATATc                                  <  2:791032/150‑1 (MQ=255)                                                                                                                   ggATGCTATCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGTGGCGGCAAAAAACCGAATTATCATGCCGATGATATcgcc                               <  1:242719/151‑1 (MQ=255)                                                                                                                   ggATGCTATCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGTGGCGGCAAAAAACCGAATTATCATGCCGATGATATcgc                                <  2:489340/150‑1 (MQ=255)                                                                                                                    gATGCTATCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGTGGCGGCAAAAAACCGAATTATCATGCCGATGATATcgccg                              <  1:552406/151‑1 (MQ=255)                                                                                                                       gCTATCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGTGGCGGCAAAAAACCGAATTATCATGCCGATGATATCGCCGCAg                           <  1:629345/151‑1 (MQ=255)                                                                                                                          aTCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGTGGCGGCAAAAAACCGAATTATCATGCCGATGATATCGCCGCAGCCt                        >  1:47054/1‑151 (MQ=255)                                                                                                                          aTCCGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGTGGCGGCAAAAAACCGAATTATCATGCCGATGATATCGCCGCAGCCt                        >  1:112146/1‑151 (MQ=255)                                                                                                                            ccGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGTGGCGGCAAAAAACCGAATTATCATGCCGATGATATCGCCGCAGCCTg                       <  1:1046991/150‑1 (MQ=255)                                                                                                                            ccGCGCAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGTGGCGGCAAAAAACCGAATTATCATGCCGATGATATCGCCGCAGCCTGc                      <  1:710744/151‑1 (MQ=255)                                                                                                                                gcAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGTGGCGGCAAAAAACCGAATTATCATGCCGATGATATCGCCGCAGCCTGCGAt                   >  1:733815/1‑150 (MQ=255)                                                                                                                                gcAGCCCGCGCCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGTGGCGGCAAAAAACCGAATTATCATGCCGATGATATCGCCGCAGCCTGCGATa                  <  1:166944/151‑1 (MQ=255)                                                                                                                                        cgcCAGCCATATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGTGGCGGCAAAAAACCGAATTATCATGCCGATGATATCGCCGCAGCCTGCGATACGCTGCAc          >  1:738585/1‑151 (MQ=255)                                                                                                                                         gccagccaTATGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGTGGCGGCAAAAAACCGAATTATCATGCCGATGATATCGCCGCAGCCTGCGATACGCTGCACg         <  1:86990/151‑1 (MQ=255)                                                                                                                                                atatGCTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGTGGCGGCAAAAAACCGAATTATCATGCCGATGATATCGCCGCAGCCTGCGATACGCTGCACGAGTTTGa  <  2:641483/151‑1 (MQ=255)                                                                                                                                                 tatGCTCCGCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGAACAGCGGCGACCCGTATGGTGATATTATTATGCGTGGCGGCAAAAAACCGAATTACCATGCGAATGATATCGCCGCAGCCTTCGATACGCTGCACGAGttt    <  2:750484/148‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  GGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGTGGCGGCAAAAAACCGAATTATCATGCCGATGATATCGCCGCAGCCTGCGATACGCTGCACGAGTTTGA  >  NC_000913/1788908‑1789201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑