Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,882,431 G→A A174T (GCC→ACC)  dmlA → D‑malate oxidase, NAD‑dependent; putative tartrate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,882,4310GA98.4% 237.9 / NA 63A174T (GCC→ACC) dmlAD‑malate oxidase, NAD‑dependent; putative tartrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (34/28);  minor base T (0/1);  total (34/29)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGCGACATCGATTTTTACGTGGTCAGGGAAAACACCGAAGGCGAATATTCCTCGCTCGGCGGTAGAGTGAATGAAGGTACAGAGCATGAAGTCGTCATTCAGGAATCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATGCCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGACAAGCAGCAT  >  NC_000913/1882283‑1882577
                                                                                                                                                    |                                                                                                                                                  
tGGCGACATCGATTTTTACGTGGTCAGGGAAAACACCGAAGGCGAATATTCCTCGCTCGGCGGCAGAGTGAATGAAGGTACAGAGCATGAAGTCGTCATTCAGGAATCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATAcc                                                                                                                                                  >  1:616747/1‑151 (MQ=255)
 ggCGACATCGATTTTTACGTGGTCAGGGAAAACACCGAAGGCGAATATTCCTCGCTCGGCGGTAGAGTAAATGAAGGTACAGAGCATGAAGTCGTCATTCAGGAATCGGTATTTCCCCGCCGTGGTGTCGATCGCATTTTGCGTTATAcc                                                                                                                                                  <  1:442786/150‑1 (MQ=255)
   cgccATCGATTTTTGGGTGGCCAGGGAAAACACCGAAGGCGAATATCCCTCGCTCGGCGGTAGAGTGAAGGAAGGTACAGAGCATGAAGTCTTCATTCAGGATTCGTTATTTCCCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCtt                                                                                                                                                <  1:414798/147‑1 (MQ=255)
    gACATCGATTTTTACGTGGTCAGGGAAAACACCGAAGGCGAATATTCCTCGCTCGGCGGTAGAGTGAATGAAGGTACAGAGCATGAAGTCGTCATTCAGGAATCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCg                                                                                                                                              >  1:289177/1‑151 (MQ=255)
     aCATCGATTTTTACGTGGTCAGGAAAACCCCCGAAGGCGAATATTCCTCGCTCGGGGGTAGAGTGAATGAAGGTACAGAGCATGAAGTCGTCATTCAGGAATCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTc                                                                                                                                               <  1:720615/149‑1 (MQ=255)
         cgttttttGCGGGGTCAGGAAAAACACCGAGGGCAAATATTCCCCGCGGGGCGGAAGAGTGAATGAGGGTACAGAGAATGAAGTCTTCATTCAGAAACCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGa                                                                                                                                             <  2:493352/144‑1 (MQ=255)
         cGATTTTTACTTGGCCAGGGAAAACCCCGAAGGCGAATGTTCCTCGCTCGGCGGTAGAGTGAATGAAGGTACAGGTCATGAAGTCGTCTTTCAGGAATCGTTATTTCCCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACt                                                                                                                                          <  2:792700/150‑1 (MQ=255)
         cGATTTTTACGTGGTCAGGGAAAACCCCGAAGGGGAATATTCCTCGCTCGGCGGTAGAGTGAATGAAGGTACAGAGCATGAAGTCGTCTTGCAGGAATCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACt                                                                                                                                          <  2:253201/150‑1 (MQ=255)
          gATTTTTACGTGGTCAGGGAAAACACCGAAGGCGAATATTCCTCGCTCGGCGGTAGAGTGAATGAAGGTACAGAGCATGAAGTCGTCATTCAGGAATCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTg                                                                                                                                        >  2:261539/1‑151 (MQ=255)
                     ggTCAGGAAAACCACCGAAGGCGAATATTCCTCGCTGGGGGGTAGAGTGAATGAAGGTACAGAGAATGAGGTCGTCTTTCAGGATTCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCg                                                                                                                              <  1:975413/150‑1 (MQ=255)
                               aaCCCCGAAGGCGAATATTCCTCGCTCGGCGGTAGAGTGAATGAAGGTACAGAGCATGAAGTCGTCATTCAGAAATCGGTTTTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGa                                                                                                                   <  1:397391/151‑1 (MQ=255)
                                   ccGAAGGCGAATTTTCCTCGCTCGGCGGTAGATTAAATGAAGGTACAGAGCATGAAGTCGTCATTCAGGAATCGGTTTTTCCCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGaca                                                                                                                 <  2:793317/149‑1 (MQ=255)
                                     gAAGGCGAATATTCCTCGCTCGGCGGTAGAGTGAATGAAGGTACAGAGCATGAAGTCGTCATTCAGGAATCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTa                                                                                                              >  1:322511/1‑150 (MQ=255)
                                      aaGGCGAATATTCCTCGCTCGGGGGTAGAGTGAATGAAGGTACAGAGCAGGAAGTCTTCATTCAGGAATCGTTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAAc                                                                                                            <  1:649390/151‑1 (MQ=255)
                                           gAATATCCCTCGCTGGGCGGTAGAGTAAATAAAGGTACAGAGCATGAAGTCGTCATTCAGGAATCGTTATTTCCCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCt                                                                                                        <  2:703856/150‑1 (MQ=255)
                                            aatttcccTCGCTCGGCGGTAGAGTGAATGAAGGTACAGAGCATGAAGTCGTCATTCAGGAATCGTTATTTCCCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTg                                                                                                       <  2:64120/144‑1 (MQ=255)
                                                 tCCTCGCTCGGCGGTAGAGTGAATGAAGGTACAGAGCATGAAGTCGTCATTCAGGAATCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACt                                                                                                  >  1:844156/1‑150 (MQ=255)
                                                  ccTCGCTCGGCGGTAGAGTGAATGAAGGTACAGAGCATGAAGTCGTCATTCAGGAATCGGTATTTACCCGCCGTGGTGTCGCTCGCATTTTGCGTTATACCTTCGAACTTGCGCAAACCCGTCCACGTAAGACACTAACTTCTGCCACTa                                                                                                 >  2:821688/1‑150 (MQ=255)
                                                       ctcgGCGGTAGAGGGAATGAAGGTACAGAGCATGAAGTCGTCATTCAGGAAGCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGGCACTAAATTTTTCCACTaaa                                                                                               >  2:127212/1‑147 (MQ=255)
                                                          ggcggTAGAGTGAATGAAGGTACAGAGCATGAAGTCGTCATTCAGGAATCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACg                                                                                        >  1:690475/1‑151 (MQ=255)
                                                           gcggTAGAGTGAATGAAGGTACAGAGCATGAAGTCGTCATTCAGGAATCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACgg                                                                                       >  1:171469/1‑151 (MQ=255)
                                                             ggTAGAGTGAATGAAGGTACAGAGCATGAAGTCGTCATTCAGGAATCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGtt                                                                                     >  2:501394/1‑151 (MQ=255)
                                                               tAGAGTGAATGAAGGTACAGAGCATGAAGTCGTCATTCAGGAATCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGttt                                                                                    >  2:47212/1‑150 (MQ=255)
                                                                agagTGAATGAAGGTACAGAGCATGAAGTCGTCATTCAGGAATCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGttt                                                                                    >  2:98798/1‑149 (MQ=255)
                                                                 gggtaaagaagggTAAAGAGAATAAAGTCTTCATACAGAAATGGGTTTTTCCCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTa                                                                                   <  2:580807/138‑1 (MQ=255)
                                                                   gTGAATAAAGGTACAGAGCATGAAGTCGTCATTCAGGAATCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGcc                                                                                <  1:857492/150‑1 (MQ=255)
                                                                     gaatgaaGGTACAGAGCATGAAGTCGTCATTCAGGAATCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATc                                                                             >  2:807324/1‑151 (MQ=255)
                                                                        tgaaGGTACAGAGCATGAAGTCGTCATTCAGGAATCGGTATTTACCCGCCCTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGc                                                                          >  1:896001/1‑151 (MQ=255)
                                                                         gaaGGTACAGAGCATGAAGTCGTCATTCAGGAATCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGc                                                                          >  1:597114/1‑150 (MQ=255)
                                                                          aaGGTACAGAGCATGAAGTCGCCATTCAGGAATCGTTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCa                                                                         <  1:266809/150‑1 (MQ=255)
                                                                                      aTGAAGTCGTCATTCGGGAATCGTTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTgg                                                              <  1:211808/149‑1 (MQ=255)
                                                                                        gAAGTCGTCATTCAGGAATCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATg                                                          >  1:740592/1‑151 (MQ=255)
                                                                                            tcgtcATTCAGGAATCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCACGCCGTACTGGGATGAGCg                                                      >  2:178601/1‑151 (MQ=255)
                                                                                             cgtcATTCAGGAATCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCg                                                      >  1:14030/1‑150 (MQ=255)
                                                                                             cgtcATTCAGGAATCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCg                                                      >  2:384958/1‑150 (MQ=255)
                                                                                              gtcATTCAGGAATCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCg                                                      >  1:262182/1‑149 (MQ=255)
                                                                                                 aTTCAGGAATCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTgg                                                 >  2:326803/1‑151 (MQ=255)
                                                                                                  ttCAGGAAGCGTTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGa                                                     <  1:522351/146‑1 (MQ=255)
                                                                                                   tCAGGAATCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGa                                                >  2:346305/1‑150 (MQ=255)
                                                                                                     aGGAATCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGc                                             >  1:313091/1‑151 (MQ=255)
                                                                                                      ggAATGGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGc                                             <  1:551651/150‑1 (MQ=255)
                                                                                                      ggAATCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGc                                             >  1:940141/1‑150 (MQ=255)
                                                                                                        aaTCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAAt                                          >  1:387112/1‑151 (MQ=255)
                                                                                                            ggTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGc                                       <  2:384074/150‑1 (MQ=255)
                                                                                                             gTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCg                                     >  1:182257/1‑151 (MQ=255)
                                                                                                               aTTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCg                                     >  2:902051/1‑149 (MQ=255)
                                                                                                                tttACCCGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGc                                       <  2:73649/146‑1 (MQ=255)
                                                                                                                   aCCCGCCGGGGTGTCGATCGCATTTTGCTTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGaa                                 <  2:390031/149‑1 (MQ=255)
                                                                                                                    cccGCCGTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTa                              <  1:767456/151‑1 (MQ=255)
                                                                                                                       gccgTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTAccc                           >  1:1011084/1‑151 (MQ=255)
                                                                                                                        ccgTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCg                          <  1:848402/151‑1 (MQ=255)
                                                                                                                         cgTGGTGTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCgg                         >  1:732980/1‑151 (MQ=255)
                                                                                                                           tggtgTCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCgg                         >  1:681966/1‑149 (MQ=255)
                                                                                                                               gtCGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGAtac                   >  2:704812/1‑149 (MQ=255)
                                                                                                                                 cGATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGc                 >  2:504218/1‑151 (MQ=255)
                                                                                                                                  gATCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCt                >  1:934507/1‑151 (MQ=255)
                                                                                                                                   aTCGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTg               >  1:611902/1‑151 (MQ=255)
                                                                                                                                     cGCATTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTggg             <  1:403626/151‑1 (MQ=255)
                                                                                                                                        aTTTTGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGAct          <  2:24985/151‑2 (MQ=255)
                                                                                                                                         ttttGCGTTATTCCTTCGAGCTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCGCTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGACa          <  2:400498/150‑1 (MQ=255)
                                                                                                                                          tttGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGACaa         <  2:828957/150‑1 (MQ=255)
                                                                                                                                           ttGCGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGACAag        <  1:93017/150‑1 (MQ=255)
                                                                                                                                              cGTTATACCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGACAagcagc    <  2:954349/151‑1 (MQ=255)
                                                                                                                                                 tataCCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGACAAGCAGCat  >  1:295419/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TGGCGACATCGATTTTTACGTGGTCAGGGAAAACACCGAAGGCGAATATTCCTCGCTCGGCGGTAGAGTGAATGAAGGTACAGAGCATGAAGTCGTCATTCAGGAATCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATGCCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACACTAACTTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACCCGGAGATCCGCTGGGACAAGCAGCAT  >  NC_000913/1882283‑1882577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑