Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,897,602	T→C	M224T (ATG→ACG)	sdaA →	L‑serine dehydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,897,602	0	T	C	100.0%	184.5 / NA	51	M224T (ATG→ACG)	sdaA	L‑serine dehydratase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/20);  total (31/20)

CGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGACTGGGTAAACATGTTTGCGCTGGCAGTTAACG  >  NC_000913/1897456‑1897748                                                                                                                                                   |                                                                                                                                                   tgAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGCCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGaa                                                                                                                                                 >  2:619742/2‑150 (MQ=255)    aCTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACAcc                                                                                                                                             <  1:68840/151‑1 (MQ=255)     cTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCg                                                                                                                                            >  1:947085/1‑151 (MQ=255)      tGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCg                                                                                                                                            >  2:40908/1‑150 (MQ=255)          cgcgTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGgt                                                                                                                                       <  2:203921/151‑1 (MQ=255)                 tGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGc                                                                                                                                >  2:401361/1‑151 (MQ=255)                    aaTGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGcc                                                                                                                               >  2:519353/1‑149 (MQ=255)                    aaTGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGcc                                                                                                                               >  1:631768/1‑149 (MQ=255)                        aaaCCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGccc                                                                                                                         <  1:504094/151‑1 (MQ=255)                            cGGCTAATCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGGGTACTGCCAGGCCCGCTg                                                                                                                     >  2:309695/1‑151 (MQ=255)                             ggCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTgc                                                                                                                    <  1:755163/151‑1 (MQ=255)                                  ttCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTg                                                                                                               >  1:34768/1‑151 (MQ=255)                                     gCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGcc                                                                                                             >  2:352203/1‑150 (MQ=255)                                     gCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGcc                                                                                                             >  2:832080/1‑150 (MQ=255)                                         tCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCAcgtc                                                                                                        >  1:457141/1‑151 (MQ=255)                                           tGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCAcgtcgt                                                                                                      >  2:559652/1‑151 (MQ=255)                                            ggTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCAcgtc                                                                                                        <  2:613351/148‑1 (MQ=255)                                                   cTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGc                                                                                              >  1:877981/1‑151 (MQ=255)                                                   cTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGc                                                                                              >  2:919775/1‑151 (MQ=255)                                                        cAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTgc                                                                                         <  1:726303/151‑1 (MQ=255)                                                         aGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTgcg                                                                                        <  1:729837/151‑1 (MQ=255)                                                                     cgcTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGt                                                                            <  2:690667/151‑1 (MQ=255)                                                                                 agaaagaGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATaa                                                                <  1:134924/151‑1 (MQ=255)                                                                                  gaaagaGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATaa                                                                >  1:505117/1‑150 (MQ=255)                                                                                   aaagaGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAAc                                                              >  1:994210/1‑151 (MQ=255)                                                                                   aaagaGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAAc                                                              <  1:216331/151‑1 (MQ=255)                                                                                   aaagaGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAAc                                                              >  1:293713/1‑151 (MQ=255)                                                                                         aTCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCt                                                         >  1:399433/1‑150 (MQ=255)                                                                                          tCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGGGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTa                                                        >  2:998272/1‑150 (MQ=255)                                                                                           cgacgaGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTaa                                                       <  2:936513/150‑1 (MQ=255)                                                                                               gaGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGAt                                                   <  1:200938/150‑1 (MQ=255)                                                                                                aGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATcc                                                 <  2:204955/151‑1 (MQ=255)                                                                                                   aTTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGa                                               <  1:787778/150‑1 (MQ=255)                                                                                                   aTTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGAt                                              >  2:109149/1‑151 (MQ=255)                                                                                                   aTTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGAt                                              >  1:566392/1‑151 (MQ=255)                                                                                                        gcgcATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATg                                         >  1:14496/1‑151 (MQ=255)                                                                                                          gcATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTc                                       >  1:905916/1‑151 (MQ=255)                                                                                                            aTGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCAt                                     >  1:897940/1‑151 (MQ=255)                                                                                                                  ggCAAACCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGa                                  <  2:1010247/148‑1 (MQ=255)                                                                                                                     aaaCCATGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGACTGGGt                            >  2:375861/1‑151 (MQ=255)                                                                                                                           tGCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGACTGGGTAAACAt                      >  1:1069701/1‑151 (MQ=255)                                                                                                                            gCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGACTGGGTAAACAt                      <  2:578643/150‑1 (MQ=255)                                                                                                                            gCAGGCATGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGACTGGGTAAACATg                     >  2:763378/1‑151 (MQ=255)                                                                                                                                   tGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGACTGGGTAAACATGTTTgcgc              >  2:894638/1‑151 (MQ=255)                                                                                                                                   tGTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGACTGGGTAAACATGTTTg                 >  2:936569/1‑148 (MQ=255)                                                                                                                                    gTATCGATCGCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGACTGGGTAAACATGTTTGCGCt             <  1:90539/151‑1 (MQ=255)                                                                                                                                      atcgatcgCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGACTGGGTAAACATGTTTGCGCTgg           <  2:535081/151‑1 (MQ=255)                                                                                                                                       tcgatcgCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGACTGGGTAAACATGTTTGCGCTgg           <  2:354707/150‑1 (MQ=255)                                                                                                                                         gatcgCGGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGACTGGGTAAACATGTTTGCGCTGGc          >  2:476718/1‑149 (MQ=255)                                                                                                                                             gcgGGACGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGACTGGGTAAACATGTTTGCGCTGGCAGTTa     >  1:474975/1‑150 (MQ=255)                                                                                                                                               gggACGAGCCCCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGACTGGGTAAACATGTTTGCGCTGGCAGTTAACg  <  2:58747/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CGAACTGCTCGCGTACTGTAATGAAACCGGCTATTCGCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGACTGGGTAAACATGTTTGCGCTGGCAGTTAACG  >  NC_000913/1897456‑1897748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑