Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,937,537	T→C	intergenic (+16/‑112)	yebK → / → pykA	putative DNA‑binding transcriptional regulator/pyruvate kinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,937,537	0	T	C	100.0%	247.7 / NA	64	intergenic (+16/‑112)	yebK/pykA	putative DNA‑binding transcriptional regulator/pyruvate kinase II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/33);  total (31/33)

TAGATGTGCTGGCGACAGGATTTACTTTGCGACGCGGTGCAAAATTCAGAGATAACTTGAAGCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGTCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACC  >  NC_000913/1937391‑1937684                                                                                                                                                   |                                                                                                                                                    tAGATGTGCTGGCGACAGGATTTACTTTGCGACGCGGTGCAAAATTCAGAGATAACTTGAAGCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCt                                                                                                                                                  >  1:341561/1‑150 (MQ=255)  aGATGTGCTGGCGACAGGATTTACTTTGCGACGCGGTGCAAAATTCAGAGATAACTTGAAGCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTaa                                                                                                                                                <  2:843848/151‑1 (MQ=255)  aGATGTGCTGGCGACAGGATTTACTTTGCGACGCGGTGCAAAATTCAGAGATAACTTGAAGCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTa                                                                                                                                                 <  1:590207/150‑1 (MQ=255)   gATGTGCTGGCGACAGGATTTACTTTGCGACGCGGTGCAAAATTCAGAGATAACTTGAAGCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAAt                                                                                                                                               <  1:806696/151‑1 (MQ=255)    aTGTGCTGGCGACAGGATTTACTTTGCGACGCGGTGCAAAATTCAGAGATAACTTGAAGCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATc                                                                                                                                              >  1:1017940/1‑151 (MQ=255)        gCTGGCGACAGGATTTACTTTGCGACGCGGTGCAAAATTCAGAGATAACTTGAAGCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTta                                                                                                                                           <  1:894665/150‑1 (MQ=255)           ggCAACAGGATTTACTTTGCGACGCGGTGCAAAATTCAGAGATAACTTGAAGCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATAc                                                                                                                                        <  1:844232/150‑1 (MQ=255)              gACAGGATTTACTTTGCGACGCGGTGCAAAATTCAGAGATAACTTGAAGCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACa                                                                                                                                    <  1:725377/151‑1 (MQ=255)                cAGGATTTACTTTGCGACGCGGTGCAAAATTCAGAGATAACTTGAAGCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATc                                                                                                                                  >  1:914163/1‑151 (MQ=255)                   gATTTACTTTGCGACGCGGTGCAAAATTCAGAGATAACTTGAAGCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCg                                                                                                                                >  2:471585/1‑150 (MQ=255)                    aTTTACTTTGCGACGCGGTGCAAAATTCAGAGATAACTTGAAGCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGaa                                                                                                                              <  1:302166/151‑1 (MQ=255)                    aTTTACTTTGCGACGCGGTGCAAAATTCAGAGATAACTTGAAGCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGa                                                                                                                               >  1:101951/1‑150 (MQ=255)                        aCTTTGCGACGCGGTGCAAAATTCAGAGATAACTTGAAGCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATgag                                                                                                                          <  1:712244/151‑1 (MQ=255)                        aCTTTGCGACGCGGTGCAAAATTCAGAGATAACTTGAAGCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATg                                                                                                                            >  2:796982/1‑149 (MQ=255)                           ttGCGACGCGGTGCAAAATTCAGAGATAACTTGAAGCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGAt                                                                                                                        <  2:670042/150‑1 (MQ=255)                           ttGCGACGCGGTGCAAAATTCAGAGATAACTTGAAGCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGAt                                                                                                                        <  2:938075/150‑1 (MQ=255)                             gCGACGCGGTGCAAAATTCAGAGATAACTTGAAGCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTaa                                                                                                                     <  1:589649/151‑1 (MQ=255)                               gACGCGGTGCAAAATTCAGAGATAACTTGAAGCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAAtt                                                                                                                   <  2:352328/151‑1 (MQ=255)                                       gCAAAATTCAGAGATAACTTGAAGCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGcc                                                                                                            <  2:643464/150‑1 (MQ=255)                                       gCAAAATTCAGAGATAACTTGAAGCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCa                                                                                                           <  1:616494/151‑1 (MQ=255)                                         aaaaTTCAGAGATAACTTGAAGCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATc                                                                                                         <  1:471610/151‑1 (MQ=255)                                         aaaaTTCAGAGATAACTTGAAGCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATc                                                                                                         <  1:527218/151‑1 (MQ=255)                                          acaTTCAGATATACCTTGAAGCGGGTCAACGCAGCGCTGAAGGAATCCCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGCATGAGATTAATGTATCGCCa                                                                                                           >  2:383368/3‑148 (MQ=255)                                              tCAGAGATAACTTGAAGCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCgcgg                                                                                                     <  1:643848/150‑1 (MQ=255)                                                  agaTAACTTGAAGCGGGTCAACGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGAACGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCCCGGCGt                                                                                                  >  2:105553/1‑149 (MQ=255)                                                  agaTAACTTGAAGCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGtt                                                                                                 >  1:1001223/1‑150 (MQ=255)                                                  agaTAACTTGAAGCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGtt                                                                                                 >  1:504272/1‑150 (MQ=255)                                                    aTAACTTGAAGCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATGTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTAtt                                                                                              >  2:1064754/1‑151 (MQ=255)                                                      aaCTTGAAGCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTc                                                                                            <  2:49018/151‑1 (MQ=255)                                                       aCTTGAAGCGGGTCAAAGAAGCGCTGACGGAATCGCGTTTTGATATGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGCTTAATTTATCGCCATCGCGGCGTTTTTTc                                                                                            >  2:805208/1‑150 (MQ=255)                                                         ttGAAGCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCAtt                                                                                         >  1:101920/1‑151 (MQ=255)                                                          tGAAGCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTc                                                                                        >  2:882435/1‑151 (MQ=255)                                                           gAAGCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTc                                                                                        <  2:76027/150‑1 (MQ=255)                                                              gCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGAt                                                                                    <  1:856401/151‑1 (MQ=255)                                                              gCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGAt                                                                                    >  2:191284/1‑151 (MQ=255)                                                               cGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGAtt                                                                                   <  1:283724/151‑1 (MQ=255)                                                                            cgcTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCaa                                                                         >  2:741475/1‑148 (MQ=255)                                                                            cgcTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGc                                                                       >  1:119134/1‑150 (MQ=255)                                                                             gcTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCaa                                                                     <  2:1006160/151‑1 (MQ=255)                                                                             gcTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCG‑CATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAac                                                                    >  1:470964/1‑151 (MQ=255)                                                                                   ggAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTg                                                               >  2:447733/1‑151 (MQ=255)                                                                                    gAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTgg                                                              <  2:154595/151‑1 (MQ=255)                                                                                     aaTCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGgt                                                             >  2:249391/1‑151 (MQ=255)                                                                                        cgcgTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGgttg                                                           <  2:294124/150‑1 (MQ=255)                                                                                        cgcgTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGgttg                                                           <  1:155815/150‑1 (MQ=255)                                                                                          cgTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGttt                                                        <  1:113036/151‑1 (MQ=255)                                                                                                  tAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAAc                                                >  1:25814/1‑151 (MQ=255)                                                                                                   aaGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACg                                               <  2:229215/151‑1 (MQ=255)                                                                                                       aGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGt                                           >  2:430627/1‑151 (MQ=255)                                                                                                          tACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTAtt                                        >  2:535576/1‑151 (MQ=255)                                                                                                          tACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTAtt                                        >  1:232691/1‑151 (MQ=255)                                                                                                                 tttAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTc                                 >  2:954112/1‑151 (MQ=255)                                                                                                                  ttAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTcc                                >  1:909540/1‑151 (MQ=255)                                                                                                                   tAAGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTcc                                >  2:70645/1‑150 (MQ=255)                                                                                                                    aaGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCa                               <  2:999592/150‑1 (MQ=255)                                                                                                                    aaGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCa                               <  1:187828/150‑1 (MQ=255)                                                                                                                     aGTGACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGa                             <  1:884766/151‑1 (MQ=255)                                                                                                                        gACGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAgg                          <  1:377064/151‑1 (MQ=255)                                                                                                                         aCGATCGCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGc                         <  2:1001170/151‑1 (MQ=255)                                                                                                                               gCTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGc                    >  1:51450/1‑150 (MQ=255)                                                                                                                               gCTAAAAACGACTGTCACTGCCCTAATATTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGc                    >  2:968925/1‑150 (MQ=255)                                                                                                                                cTAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGc                    >  1:287648/1‑149 (MQ=255)                                                                                                                                 tAAAAACGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGc                    >  2:595431/1‑148 (MQ=255)                                                                                                                                  aaaaaCGACTGTCACTGCCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAg                  <  2:897622/149‑1 (MQ=255)                                                                                                                                           tgtcactgccCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCg       >  1:991110/1‑151 (MQ=255)                                                                                                                                           tgtcactgccCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCg       >  1:2739/1‑151 (MQ=255)                                                                                                                                            gtcactgccCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCg       <  1:1069646/150‑1 (MQ=255)                                                                                                                                            gtcactgccCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGt      <  1:662823/151‑1 (MQ=255)                                                                                                                                            gtcactgccCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGt      >  1:720324/1‑151 (MQ=255)                                                                                                                                             tcactgccCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCATAAGGCTTCGCAGAACAAAAATCGt      <  1:662857/150‑1 (MQ=255)                                                                                                                                              cactgccCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGtt     <  2:909493/150‑1 (MQ=255)                                                                                                                                                ctgccCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTAcc  >  1:29372/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                    TAGATGTGCTGGCGACAGGATTTACTTTGCGACGCGGTGCAAAATTCAGAGATAACTTGAAGCGGGTCAAAGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAAAAACGACTGTCACTGTCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGTTTCAGTCAACGGAGTATTACATGTCCAGAAGGCTTCGCAGAACAAAAATCGTTACC  >  NC_000913/1937391‑1937684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑