Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,952,097 A→G T235A (ACG→GCG)  yecE → UPF0759 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,952,0970AG100.0% 229.7 / NA 60T235A (ACG→GCG) yecEUPF0759 family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/20);  total (40/20)

CGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATACGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCTTCCAGAGATCGGAGCAGTTCCGGCTATTCCACAGCAATCTTCTCTTTTCTGAATTTGCCACCTATCATAGACAGGTGCCATCG  >  NC_000913/1951951‑1952244
                                                                                                                                                  |                                                                                                                                                   
cGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGcc                                                                                                                                                 >  1:137706/1‑151 (MQ=255)
cGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGcc                                                                                                                                                 >  2:20501/1‑151 (MQ=255)
        gCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTg                                                                                                                                         <  2:336955/151‑1 (MQ=255)
        gCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTg                                                                                                                                         >  1:229733/1‑151 (MQ=255)
        gCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTg                                                                                                                                         <  1:831151/151‑1 (MQ=255)
         cTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGc                                                                                                                                        >  1:87473/1‑151 (MQ=255)
          tGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACAtt                                                                                                                                          <  2:926356/148‑1 (MQ=255)
                gACGGCGACAAATCCGCTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGc                                                                                                                                  >  2:26819/1‑150 (MQ=255)
                gACGGCGACAAATCCGCTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGc                                                                                                                                  >  1:278795/1‑150 (MQ=255)
                    gCGACAAATCCGCTGATCCGTTTTATCGGTAGTGATGATATGACGTAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGTAGTGGCATCAGACCACTGCGCCTTATCTTTTTTTACATGCGTCAGACATTGCCCAGGCCCCgg                                                                                                                             >  2:332407/1‑151 (MQ=255)
                    gCGACAAATCCGCTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCgg                                                                                                                             >  2:811252/1‑151 (MQ=255)
                    gCGACAAATCCGCTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCgg                                                                                                                             >  2:136251/1‑151 (MQ=255)
                     cGACAAATCAGCAGATCCGTTATATCGGTAGTGACGATATGACGCACAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGACATCAGACGACTACGCCTTATCTTTTTTTACATGTGCAAGACTATGCCCAgt                                                                                                                                   >  2:459558/1‑143 (MQ=255)
                          aaTCCGCTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTgg                                                                                                                       <  2:704616/151‑1 (MQ=255)
                            tCCGCTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGt                                                                                                                      >  1:1047852/1‑150 (MQ=255)
                                  gATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATa                                                                                                                 <  2:687645/149‑1 (MQ=255)
                                     ccGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCGCTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCt                                                                                                             <  1:638622/150‑1 (MQ=255)
                                              cGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGAc                                                                                                   >  1:1059496/1‑151 (MQ=255)
                                                gTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACtt                                                                                                 >  1:174188/1‑151 (MQ=255)
                                                 tAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACtt                                                                                                 >  2:389912/1‑150 (MQ=255)
                                                  aGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTAc                                                                                               <  2:300454/151‑1 (MQ=255)
                                                  aGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTAc                                                                                               >  1:119857/1‑151 (MQ=255)
                                                    tgatgaTATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGt                                                                                             >  1:551707/1‑151 (MQ=255)
                                                    tgatgaTATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGt                                                                                             >  1:275058/1‑151 (MQ=255)
                                                     gatgatATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGt                                                                                             >  2:955316/1‑150 (MQ=255)
                                                         atatGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAAc                                                                                        >  1:1022619/1‑151 (MQ=255)
                                                             gACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCtt                                                                                    >  1:144490/1‑151 (MQ=255)
                                                               cGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCTTcc                                                                                  <  2:900187/151‑1 (MQ=255)
                                                                    aaCCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCTTCCag                                                                                >  2:292909/1‑148 (MQ=255)
                                                                     aCCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCTTCCag                                                                                >  2:850421/1‑147 (MQ=255)
                                                                      ccGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCTTCCag                                                                                >  1:759809/1‑146 (MQ=255)
                                                                      ccGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCTTCCAGAGATc                                                                           <  1:995748/151‑1 (MQ=255)
                                                                          gAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCTTCCAGAGATCGGAg                                                                       <  2:285318/151‑1 (MQ=255)
                                                                           aaTTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCTTCCAGAGATCGGAGc                                                                      <  2:416562/151‑1 (MQ=255)
                                                                               attTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCTTCCAGAGATCGGAGCAGtt                                                                  >  1:279741/1‑151 (MQ=255)
                                                                                 ttCAGGTCTGGTTACAAAAATTATCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACTTAAAACGCTTCCAGAGATCGGAGCAGTTcc                                                                >  2:761735/1‑151 (MQ=255)
                                                                                 ttCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCTTCCAGAGATCGGAGCAGTTcc                                                                >  1:708525/1‑151 (MQ=255)
                                                                                  tCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCTTCCAGAGATCGGAGCAGTTcc                                                                <  2:312086/150‑1 (MQ=255)
                                                                                   cAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCTTCCAGAGATCGGAGCAGTTCCg                                                               <  1:73192/150‑1 (MQ=255)
                                                                                    aGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCTTCCAGAGATCGGAGCAGTTCCGGc                                                             >  1:971070/1‑151 (MQ=255)
                                                                                      gTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCTTCCAGAGATCGGAGCAGTTCCGGCt                                                            >  2:1005012/1‑150 (MQ=255)
                                                                                        cTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCTTCCAGAGATCGGAGCAGTTCCGGCTAtt                                                         >  1:243167/1‑151 (MQ=255)
                                                                                          ggTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCTTCCAGAGATCGGAGCAGTTCCGGCTAt                                                          <  2:993856/148‑1 (MQ=255)
                                                                                          ggTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCTTCCAGAGATCGGAGCAGTTCCGGCTATTc                                                        <  1:90024/150‑1 (MQ=255)
                                                                                            ttACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCTTCCAGAGATCGGAGCAGTTCCGGCTATTCcac                                                     >  2:667940/1‑151 (MQ=255)
                                                                                                 aaaaTTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCTTCCAGAGATCGGAGCAGTTCCGGCTATTCCACAGCa                                                 >  2:474522/1‑150 (MQ=255)
                                                                                                  aaaTTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCTTCCAGAGATCGGAGCAGTTCCGGCTATTCCACAGCAAt                                               >  2:896468/1‑151 (MQ=255)
                                                                                                   aaTTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCTTCCAGAGATCGGAGCAGTTCCGGCTATTCCACAGCAAtc                                              >  1:1048525/1‑151 (MQ=255)
                                                                                                           cAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCTTCCAGAGATCGGAGCAGTTCCGGCTATTCCACAGCAATCTTCTCttt                                      <  2:585831/151‑1 (MQ=255)
                                                                                                                     aGACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCTTCCAGAGATCGGAGCAGTTCCGGCTATTCCACAGCAATCTTCTCTTTTCTGAATTTg                            <  2:270390/151‑1 (MQ=255)
                                                                                                                      gACCACTACGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCAGAACTGGTACATGCCCTGTGGGAAGACTTACGTAAATCGCTTCCAGAGATCGGAGCAGTTCCGGCTATTCCACAGCAATCTTCTCTTTTCTGAATTTGc                           <  2:278289/151‑1 (MQ=255)
                                                                                                                             aCGCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCCTCCAGAGATCGGAGCAGTTCCGGCTATTCCACAGCAATCTTCTCTTTTCTGAATTTGCCACCt                      <  2:866363/149‑1 (MQ=255)
                                                                                                                               gCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCTTCCAGAGATCGGAGCAGTTCCGGCTATTCCACAGCa                                                 >  1:857265/1‑120 (MQ=255)
                                                                                                                               gCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCTTCCAGAGATCGGAGCAGTTCCGGCTATTCCACAGCa                                                 <  2:857217/120‑1 (MQ=255)
                                                                                                                               gCCTTATCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCTTCCAGAGATCGGAGCAGTTCCGGCTATTCCACAGCAATCTTCTCTTTTCTGAATTTGCCACCTATc                   >  2:307197/1‑150 (MQ=255)
                                                                                                                                    aTCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCTTCCAGAGATCGGAGCAGTTCCGGCTATTCCACAGCAATCTTCTCTTTTCTGAATTTGCCACCTATCATAGAc             >  2:842119/1‑151 (MQ=255)
                                                                                                                                    aTCTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCTTCCAGAGATCGGAGCAGTTCCGGCTATTCCACAGCAATCTTCTCTTTTCTGAATTTGCCACCTATCATAGAc             >  1:384915/1‑151 (MQ=255)
                                                                                                                                      cTTTTTTTACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCTTCCAGAGATCGGAGCAGTTCCGGCTATTCCACAGCAATCTTCTCTTTTCTGAATTTGCCACCTATCATAGAc             >  2:179778/1‑149 (MQ=255)
                                                                                                                                             tACATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCTTCCAGAGATCGGAGCAGTTCCGGCTATTCCACAGCAATCTTCTCTTTTCTGAATTTGCCACCTATCATAGACAGGTGCCAt    >  1:889634/1‑151 (MQ=255)
                                                                                                                                               cATGCGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCTTCCAGAGATCGGAGCAGTTCCGGCTATTCCACAGCAATCTTCTCTTTTCTGAATTTGCCACCTATCATAGACAGGTGCCATCg  >  1:967876/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CGGTACATGCTGTACTGACGGCGACAAATCCGCTGATCCGTTTTATCGGTAGTGATGATATGACGCAAAACCGGGAATTATTTCAGGTCTGGTTACAAAAATTAGCGCAGTGGCATCAGACCACTACGCCTTATCTTTTTTTACATACGCCAGACATTGCCCAGGCACCGGAACTGGTACATACCCTGTGGGAAGACTTACGTAAAACGCTTCCAGAGATCGGAGCAGTTCCGGCTATTCCACAGCAATCTTCTCTTTTCTGAATTTGCCACCTATCATAGACAGGTGCCATCG  >  NC_000913/1951951‑1952244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: