Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,993,219	A→G	V496V (GTT→GTC)	uvrC ←	excinuclease UvrABC, endonuclease subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,993,219	0	A	G	100.0%	173.3 / NA	50	V496V (GTT→GTC)	uvrC	excinuclease UvrABC, endonuclease subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/28);  total (22/28)

CCCGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCAACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTGTCGTCAATGGCTTTACCATAA  >  NC_000913/1993075‑1993360                                                                                                                                                 |                                                                                                                                              cccGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTGCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCa                                                                                                                                         <  2:228977/151‑1 (MQ=255)  ccGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCaa                                                                                                                                        <  1:514313/151‑1 (MQ=255)   cGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAg                                                                                                                                       <  1:969288/151‑1 (MQ=255)     ccAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGt                                                                                                                                      >  1:801023/1‑150 (MQ=255)      cAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGt                                                                                                                                      >  1:427927/1‑149 (MQ=255)       aaTCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTagc                                                                                                                                   >  2:593492/1‑151 (MQ=255)        aTCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTagc                                                                                                                                   >  1:1047243/1‑150 (MQ=255)          cgcgTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTagcagc                                                                                                                                >  2:588586/1‑151 (MQ=255)          cgcgTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTagcag                                                                                                                                 <  1:709128/150‑1 (MQ=255)             gTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCgg                                                                                                                              >  1:923336/1‑150 (MQ=255)               gATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGgat                                                                                                                            <  2:141552/150‑1 (MQ=255)               gATCATGTGATTCATCGCGAATATGCTGGATAACATGCAACGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGgatg                                                                                                                           <  1:316631/151‑1 (MQ=255)                 tCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGgatgat                                                                                                                         >  2:243589/1‑151 (MQ=255)                          tCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATccc                                                                                                                >  2:228064/1‑151 (MQ=255)                             tCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATg                                                                                                             >  2:499866/1‑151 (MQ=255)                              cgcgAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATga                                                                                                            <  1:819186/151‑1 (MQ=255)                              cgcgAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATg                                                                                                             <  2:644569/150‑1 (MQ=255)                                    tatGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATc                                                                                                      >  2:1033833/1‑151 (MQ=255)                                     atGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATcc                                                                                                     <  2:427901/151‑1 (MQ=255)                                     atGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTCCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATc                                                                                                      <  2:185645/150‑1 (MQ=255)                                       gCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATcc                                                                                                     >  1:327923/1‑149 (MQ=255)                                          ggATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGtt                                                                                                 <  1:793846/150‑1 (MQ=255)                                          ggATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGtt                                                                                                 <  2:1021405/150‑1 (MQ=255)                                          ggATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTc                                                                                                <  2:292462/151‑1 (MQ=255)                                              aaCATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGc                                                                                             <  2:540498/150‑1 (MQ=255)                                                 aTGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAg                                                                                         <  2:550228/151‑1 (MQ=255)                                                    cAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGAc                                                                                       >  2:409091/1‑150 (MQ=255)                                                      gcgcGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTCCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGTCATCCAGTTCGGCGAAGCCAtt                                                                                    >  2:85961/1‑151 (MQ=255)                                                       cgcgGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACAttt                                                                                   <  1:801045/151‑1 (MQ=255)                                                         cgGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACAttttt                                                                                 >  2:972455/1‑151 (MQ=255)                                                           ggTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCg                                                                               <  1:151950/151‑1 (MQ=255)                                                            gTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGc                                                                              >  1:345045/1‑151 (MQ=255)                                                                        gcAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTg                                                                  <  1:105602/151‑1 (MQ=255)                                                                             cTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTCTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCtt                                                             >  2:230949/1‑151 (MQ=255)                                                                                    ccTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTgccgcc                                                      <  1:77605/151‑1 (MQ=255)                                                                                     cTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGccgccg                                                     >  1:875172/1‑151 (MQ=255)                                                                                           ttCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGAt                                                >  1:746501/1‑150 (MQ=255)                                                                                                gCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGAt                                          <  1:571744/151‑1 (MQ=255)                                                                                                    acaGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCCCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGCTcac                                       >  2:954195/3‑150 (MQ=255)                                                                                                      aGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATc                                    <  1:650683/151‑1 (MQ=255)                                                                                                        aaCAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATcc                                   >  1:398527/1‑150 (MQ=255)                                                                                                          cAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCggg                                >  2:662578/1‑151 (MQ=255)                                                                                                              gTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATc                             <  2:271728/150‑1 (MQ=255)                                                                                                                ttCCAGTCCAGCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCtt                           <  1:453720/150‑1 (MQ=255)                                                                                                                    agtccagCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTgt                      <  2:820564/151‑1 (MQ=255)                                                                                                                       ccagCCTTACGATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTgtcg                    <  1:826540/150‑1 (MQ=255)                                                                                                                                 gATCTGCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTGTCGTCAATGGCttt         >  2:510799/1‑151 (MQ=255)                                                                                                                                      gCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTGTCGTCAATGGCTTTa        <  2:667943/147‑1 (MQ=255)                                                                                                                                      gCTCCTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTGTCGTCAATGGCTTTAc       <  1:721251/148‑1 (MQ=255)                                                                                                                                         ccTTTGGCGACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTGTCGTCAATGGCTTTACCATaa  <  1:5406/150‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                              CCCGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTACGATCTGCTCCTTTGGCAACGCCAAGTAGCAGCGGATGATTTTTATCCCATGAGACATCCAGTTCGGCGAAGACATTTTTCGCCTGCGCAAGCTGGCCTTTGCCGCCGTCGATAAGGATCACATCCGGGATCTTACTGTCGTCAATGGCTTTACCATAA  >  NC_000913/1993075‑1993360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑