Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	262,343	G→A	G281S (GGC→AGC)	proA →	gamma‑glutamylphosphate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	262,343	0	G	A	100.0%	262.3 / NA	69	G281S (GGC→AGC)	proA	gamma‑glutamylphosphate reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/28);  total (41/28)

AGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGA  >  NC_000913/262196‑262485                                                                                                                                                    |                                                                                                                                               aGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAgcagcg                                                                                                                                             >  2:984280/1‑151 (MQ=255)     tAGTGATCGATGAAGCATTAATAGTGATCGTCCACGCGAAAACTCAGCGTCCGAGCATTTGTAATCACGTTGAAACGTTGCTGGTGAATAAAAACCTCGCCGTTAGCTTCCTGCCGGCATTAAGCAATCAAATGGCGGACAGCAGCGTGa                                                                                                                                          >  2:609500/1‑150 (MQ=255)     tAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTg                                                                                                                                           <  2:593713/149‑1 (MQ=255)        agaTCGCTGAATCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACAtt                                                                                                                                      >  2:146457/1‑151 (MQ=255)           tCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTac                                                                                                                                    >  1:308281/1‑150 (MQ=255)            cGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTacac                                                                                                                                  >  1:117286/1‑151 (MQ=255)            cGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTacac                                                                                                                                  >  1:180074/1‑151 (MQ=255)                gAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGc                                                                                                                                <  1:679858/149‑1 (MQ=255)                gAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCa                                                                                                                               <  2:995586/150‑1 (MQ=255)                gAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCa                                                                                                                               <  1:255333/150‑1 (MQ=255)                 aaGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGc                                                                                                                                >  1:949863/1‑148 (MQ=255)                     aTTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGc                                                                                                                          >  1:20609/1‑150 (MQ=255)                      ttAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGc                                                                                                                          >  1:982493/1‑149 (MQ=255)                              gATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACt                                                                                                                 <  1:713737/150‑1 (MQ=255)                                   tCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGgcgc                                                                                                            >  1:282020/1‑150 (MQ=255)                                     aaCGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGt                                                                                                         >  2:35284/1‑151 (MQ=255)                                      aCGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGtt                                                                                                        >  2:1553/1‑151 (MQ=255)                                         cgAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTgca                                                                                                     <  2:116547/151‑1 (MQ=255)                                          gAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTgc                                                                                                      >  1:71661/1‑149 (MQ=255)                                           aaaaCTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTgcagg                                                                                                   <  2:409683/151‑1 (MQ=255)                                            aaaCTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTgcaggc                                                                                                  <  1:814234/151‑1 (MQ=255)                                              aCTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTgcaggcag                                                                                                <  2:424003/151‑1 (MQ=255)                                                tCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCaggcaggc                                                                                              >  2:515084/1‑151 (MQ=255)                                                  aGCTTCCGAGCACATGTAATACGGTTGAGACTTTGCTGTTGAATAATAACATCGCCGATCTCTTCCTGCCCGAATTCATCAAACAAATGGAGCAAAGGAGTGATACCTCACATGCTGATGTAGCTGCATTGgcgcggcgtccggc                                                                                                  >  2:566305/1‑135 (MQ=255)                                                  aGCGTCCGAGCACATGTAATACGGCTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGATACAGGCAGGccc                                                                                            >  2:911667/1‑151 (MQ=255)                                                   gCGTCCGAGCAAATGTAATACGGTTGAAACGTTGCTGGGGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGAAGCTGCACTGGCGCAGTTGCAGGCAGGCCCt                                                                                           >  2:982379/1‑151 (MQ=255)                                                     gTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGc                                                                                         <  2:495491/151‑1 (MQ=255)                                                      tCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCg                                                                                        >  2:134859/1‑151 (MQ=255)                                                         gAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAg                                                                                     <  1:1054799/151‑1 (MQ=255)                                                           gCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAggt                                                                                   <  1:1051969/151‑1 (MQ=255)                                                           gCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAggt                                                                                   >  1:501486/1‑151 (MQ=255)                                                             acaTGTAATACGGTTGAAACGTTGCTGGGGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAggtgg                                                                                 >  2:1066753/1‑151 (MQ=255)                                                               aTGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGtt                                                                               <  1:578151/151‑1 (MQ=255)                                                               aTGTAATACGGATGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGtt                                                                               >  2:453446/1‑151 (MQ=255)                                                                   aaTACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTg                                                                           >  2:657512/1‑151 (MQ=255)                                                                      aCGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTa                                                                        <  1:226103/151‑1 (MQ=255)                                                                                cGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGa                                                                 >  2:1019650/1‑148 (MQ=255)                                                                                   tGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGt                                                            <  2:653640/150‑1 (MQ=255)                                                                                      tGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATg                                                         >  1:745351/1‑150 (MQ=255)                                                                                       ggTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATg                                                         <  1:381208/149‑1 (MQ=255)                                                                                          gAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGAt                                                    <  2:831131/151‑1 (MQ=255)                                                                                            aTAAAAACCTCGCCGATAGCTTCCTGCCCGCCTTAAGTCAACAAATGGCGGAAAGCAGCGTGACATTACACGAAGATGCAGCTGCACTGGCGCAGTTGCAGGCCGGCCCTGCGCAGGCGGTTGCTGTTACAGCCGAAGAGTATGACGATg                                                   >  2:264652/1‑150 (MQ=255)                                                                                              aaaaaCATCGCCGATAGCTTCCTGCCTGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGt                                                >  2:61135/1‑151 (MQ=255)                                                                                                aaaCATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGttt                                              <  2:982441/151‑1 (MQ=255)                                                                                                 aaCATCGCCGATAGCGGCCTGCCCGCATTAAGCAAACAAAAGGAGGAAAGCAGCGGGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCGCTGCGAAGGTGGTTGCTGTTAAAGCCCAAGAGTATGACGATGAGta                                               <  2:342794/149‑2 (MQ=255)                                                                                                  aCATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCt                                            >  2:634897/1‑151 (MQ=255)                                                                                                    aTCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGt                                          <  2:95042/151‑1 (MQ=255)                                                                                                        ccGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCAtt                                      <  1:1049956/151‑1 (MQ=255)                                                                                                         cGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCAtt                                      >  2:934237/1‑150 (MQ=255)                                                                                                         cGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCAtt                                      >  2:563692/1‑150 (MQ=255)                                                                                                         cGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTa                                     <  2:500581/151‑1 (MQ=255)                                                                                                                  ccTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAAc                            >  1:699433/1‑151 (MQ=255)                                                                                                                  ccTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAAc                            >  1:35164/1‑151 (MQ=255)                                                                                                                  ccTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAAc                            >  2:947756/1‑151 (MQ=255)                                                                                                                  ccTGCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGGATGACGATGAGTTTCTGTCATTAGATTTGaa                             >  2:272719/1‑150 (MQ=255)                                                                                                                    tGCCCGCATTACGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCCGCTGCACAGCCGCAGTTGCAGGCAGGCCCTGCGGAGGCGGTTGCTGTTTAAGCCGCAGAGTCTGACGGTGACCTTCTGTCATTaca                                   >  2:673234/1‑140 (MQ=255)                                                                                                                     gCCCGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAACGTc                         >  1:521956/1‑151 (MQ=255)                                                                                                                        cGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAACGTCaaa                      <  1:312806/151‑1 (MQ=255)                                                                                                                        cGCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAACGTCaa                       >  1:260627/1‑150 (MQ=255)                                                                                                                         gCATTAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAACGTCaaa                      >  1:221150/1‑150 (MQ=255)                                                                                                                             tAAGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGt                 >  1:115302/1‑151 (MQ=255)                                                                                                                              aaGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTc                <  1:455266/151‑1 (MQ=255)                                                                                                                               aGCAAACAAATGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTc                >  2:1069864/1‑150 (MQ=255)                                                                                                                                 caaacaaaTGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGc             >  2:1038599/1‑151 (MQ=255)                                                                                                                                 caaacaaaTGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGc             <  2:958271/151‑1 (MQ=255)                                                                                                                                   aacaaaTGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGa           <  2:974130/151‑1 (MQ=255)                                                                                                                                     caaaTGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATc         <  1:919058/151‑1 (MQ=255)                                                                                                                                       aaTGGCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCtt       <  2:71660/151‑1 (MQ=255)                                                                                                                                           gCGGAAAGCAGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACg   >  2:704554/1‑151 (MQ=255)                                                                                                                                             gtaaaGCAGCGTGACATTACACGCAGATGAAGCTGCGCTGACGCAGTTACAGGCAGGCCCAGCGACGGTGGTTGCTGTTAAATCCGAAGATTATGACGATGAGTTTTAGTCGTTATATTTGAACTCCGAAATCATCAGCTATCGTGACGa  >  2:44949/3‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                               AGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAGGTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGA  >  NC_000913/262196‑262485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑