Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,165,605	G→A	intergenic (+84/‑63)	yegP → / → yegQ	UPF0339 family protein/putative peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,165,605	0	G	A	100.0%	192.6 / NA	51	intergenic (+84/‑63)	yegP/yegQ	UPF0339 family protein/putative peptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/33);  total (18/33)

GGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATG  >  NC_000913/2165465‑2165752                                                                                                                                             |                                                                                                                                                    ggAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCt                                                                                                                                           >  1:254732/1‑151 (MQ=255)   aaTTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTa                                                                                                                                          >  2:508865/1‑150 (MQ=255)     ttGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAAcc                                                                                                                                       >  2:448485/1‑151 (MQ=255)              ttAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTtcatc                                                                                                                               >  1:477949/1‑150 (MQ=255)                   gCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTg                                                                                                                          >  2:548853/1‑150 (MQ=255)                    ccAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGaa                                                                                                                        <  1:739475/151‑1 (MQ=255)                         ggCACAAGCCAGACGGTGAAAGACAATACGTAATTACGAGGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGa                                                                                                                   <  2:915348/151‑1 (MQ=255)                             caAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCt                                                                                                                <  2:566122/150‑1 (MQ=255)                                  cAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTg                                                                                                          <  1:382638/151‑1 (MQ=255)                                        gTGAAAGCCAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGtcatca                                                                                                    <  2:214678/151‑1 (MQ=255)                                        gTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGtcatc                                                                                                     >  1:726509/1‑150 (MQ=255)                                          gAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAg                                                                                                   <  1:757405/150‑1 (MQ=255)                                            aaGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAAc                                                                                                >  2:260542/1‑151 (MQ=255)                                             aGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACg                                                                                               <  2:438661/151‑1 (MQ=255)                                               aCAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACgag                                                                                             <  1:853989/151‑1 (MQ=255)                                                cAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACgaga                                                                                            <  1:515574/151‑1 (MQ=255)                                                   tACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACgagaga                                                                                          >  1:368571/1‑150 (MQ=255)                                                    aCGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGaa                                                                                         <  2:1026690/150‑1 (MQ=255)                                                       tAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTAt                                                                                     >  1:824739/1‑151 (MQ=255)                                                                  ccGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGACGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCgg                                                                          >  2:39914/1‑151 (MQ=255)                                                                        gTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTcc                                                                    <  1:775604/151‑1 (MQ=255)                                                                          gggTTGTTGGCGTCCGGCGCTTTACCATAACCCTCCACAGCGCTACAATGCACGCCCTTAAAGTGGGAGCACTCCACTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTGAAACCGGAACTCCt                                                                   <  2:680958/150‑1 (MQ=255)                                                                          gggTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCtt                                                                  <  1:690651/151‑1 (MQ=255)                                                                          gggTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCtt                                                                  <  1:824464/151‑1 (MQ=255)                                                                            gttgttGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTc                                                                <  2:957000/151‑1 (MQ=255)                                                                               gttgGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTcccc                                                             <  2:254707/151‑1 (MQ=255)                                                                                 tgGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCcg                                                            <  1:4908/150‑1 (MQ=255)                                                                                 tgGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCcg                                                            <  2:1000128/150‑1 (MQ=255)                                                                                    cGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCcggcgg                                                        <  2:967093/151‑1 (MQ=255)                                                                                    cGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCcggcg                                                         <  1:93324/150‑1 (MQ=255)                                                                                        cGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAAc                                                    <  2:373903/151‑1 (MQ=255)                                                                                         ggCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACg                                                   <  2:335909/151‑1 (MQ=255)                                                                                            gcTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTg                                                >  1:480496/1‑151 (MQ=255)                                                                                            gcTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTg                                                <  1:685115/151‑1 (MQ=255)                                                                                               ttACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGaa                                              >  1:767005/1‑150 (MQ=255)                                                                                                tACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGaaa                                             >  1:928188/1‑150 (MQ=255)                                                                                                  ccAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAat                                          >  1:998263/1‑151 (MQ=255)                                                                                                  ccAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAat                                          <  1:490936/151‑1 (MQ=255)                                                                                                   cAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAat                                          >  1:499530/1‑150 (MQ=255)                                                                                                    agagCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAatat                                        <  1:301855/151‑1 (MQ=255)                                                                                                       gCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCg                                     >  2:786429/1‑151 (MQ=255)                                                                                                           cccACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTAc                                 <  2:155940/151‑1 (MQ=255)                                                                                                           cccACAGCGCTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTAc                                 <  1:47316/151‑1 (MQ=255)                                                                                                                 gcgcTACAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTc                           <  2:619774/151‑1 (MQ=255)                                                                                                                      aCAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCtt                       <  2:1014518/150‑1 (MQ=255)                                                                                                                      aCAATGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCt                        <  2:726755/149‑1 (MQ=255)                                                                                                                        aaTGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATg                    <  2:667693/151‑1 (MQ=255)                                                                                                                         aTGCCCGCCCTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATgg                   >  1:707023/1‑151 (MQ=255)                                                                                                                              cgcccTTAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGgcgc                >  1:325715/1‑149 (MQ=255)                                                                                                                                    tAAAGTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGc          <  1:309648/149‑1 (MQ=255)                                                                                                                                        gTGGGAGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTa    <  1:740475/151‑1 (MQ=255)                                                                                                                                          gggagCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATg  >  1:912408/1‑151 (MQ=255)                                                                                                                                             |                                                                                                                                                    GGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATG  >  NC_000913/2165465‑2165752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑