Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,209,439	G→A	pseudogene (340/2001 nt)	yehQ →	pseudogene

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,209,439	0	G	A	100.0%	181.4 / NA	50	pseudogene (340/2001 nt)	yehQ	pseudogene
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/27);  total (23/27)

TGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACTCAGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGAACTGGCTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGCCGGGTGAAATTCCCTCTGCCCGCTTACCGATGAGCGATGTGCGTTTTTATTCCCGCAGCAGTATT  >  NC_000913/2209293‑2209582                                                                                                                                                   |                                                                                                                                                tGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACTCAGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGAACTGACTAc                                                                                                                                             >  1:648215/1‑151 (MQ=255)  gAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACTCAGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGAACTGACTAcc                                                                                                                                            <  2:319691/151‑1 (MQ=255)  gAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACTCAGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGAACTGACTAcc                                                                                                                                            <  1:601719/151‑1 (MQ=255)            cAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACTCAGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGa                                                                                                                                    <  1:881605/149‑1 (MQ=255)                        ggggCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACTCAGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAAc                                                                                                                      <  2:879959/151‑1 (MQ=255)                            ccAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACTCAGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACgcgc                                                                                                                  <  2:5279/151‑1 (MQ=255)                              aaCGGTATGTGTCGTCATCGCGTGATGCTGGAGTTAAGTTATCAACGACTTTGTGCCACCACTCAGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGcac                                                                                                                >  2:513719/1‑151 (MQ=255)                                cGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACTCAGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAg                                                                                                              <  2:724473/151‑1 (MQ=255)                                       tgCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACTCAGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTgg                                                                                                       >  1:171144/1‑151 (MQ=255)                                        gCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACTCAGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGt                                                                                                      >  2:924745/1‑151 (MQ=255)                                                       tGCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACTCAGTCTACGGAAAAAGAAGAAGAGGGGGATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGACATCAc                                                                                       >  2:1060950/1‑151 (MQ=255)                                                        gCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACTCAGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCAc                                                                                       <  2:42342/150‑1 (MQ=255)                                                           ggTGTTAAGTTATCAACGACTTTGTGCCACCACTCAGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCAt                                                                                    <  1:915329/150‑1 (MQ=255)                                                              gtTAAGTTATCAACGACTTTGTGCCACCACTCAGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGa                                                                                 <  1:401526/150‑1 (MQ=255)                                                                 aaGTTATCAACGACTTTGTGCCACCACTCAGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAgttg                                                                             >  1:633867/1‑151 (MQ=255)                                                                   gTTATCAACGACTTTGTGCCACCACTCAGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAgttgtt                                                                           <  1:805588/151‑1 (MQ=255)                                                                    ttATCAACGACTTTGTGCCACCACTCAGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGTACTGACTACCCTTCCCGATGCTCCCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAgttgtt                                                                           <  2:598122/150‑1 (MQ=255)                                                                            gACTTTGTGCCACCACTCAGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGcc                                                                  >  2:115541/1‑151 (MQ=255)                                                                                  gtgCCACCACTCAGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGCCggg                                                               >  2:333482/1‑148 (MQ=255)                                                                                    gCCACCGCTCAGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGCCGGGTGaaa                                                          <  2:689806/151‑1 (MQ=255)                                                                                      caccacTCAGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGCCGGGTGAAAtt                                                        >  1:592799/1‑151 (MQ=255)                                                                                         cacTCAGTCTACGGAAAAAGAAGAAGAGAGGGATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGAGCGCCGGGTGAAATTccc                                                     >  2:749875/1‑151 (MQ=255)                                                                                            tCAGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGCCGGGTGAAATTCCctct                                                  >  2:560454/1‑151 (MQ=255)                                                                                             cAGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGCCGGGTGAAATTCCCTCTg                                                 <  2:436157/151‑1 (MQ=255)                                                                                             cAGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGCCGGGTGAAATTCCCTCTg                                                 <  1:1036640/151‑1 (MQ=255)                                                                                              aGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGCCGGGTGAAATTCCCTCTGc                                                >  2:139652/1‑151 (MQ=255)                                                                                              aGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGCCGGGTGAAATTCCCTCTGc                                                <  2:524295/151‑1 (MQ=255)                                                                                              aGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGCCGGGTGAAATTCCCTCTGc                                                >  2:1065377/1‑151 (MQ=255)                                                                                              aGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGCCGGGTGAAATTCCCTCTGc                                                >  2:774841/1‑151 (MQ=255)                                                                                                   aCGGAAAAAGAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCGAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGCCGGGTGAAATTCCCTCTGCCCGCt                                           <  2:601608/151‑1 (MQ=255)                                                                                                     ggAAAAAGAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGCCGGGTGAAATTCCCTCTGCCCGCtt                                          >  1:653313/1‑150 (MQ=255)                                                                                                     ggAAAAAGAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGCCGGGTGAAATTCCCTCTGCCCGCTTa                                         <  1:1011581/151‑1 (MQ=255)                                                                                                        aaaaGAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGCCGGGTGAAATTCCCTCTGCCCGCTTACCg                                      >  2:1024225/1‑151 (MQ=255)                                                                                                              agaagaGTGGGATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGCCGGGTGAAATTCCCTCTGCCCGCTTACCGATGAGc                                <  1:163430/151‑1 (MQ=255)                                                                                                                    gTGGGATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGCCGGGTGAAATTCCCTCTGCCCGCTTACCGATGAGCGAtgtg                          >  2:681834/1‑151 (MQ=255)                                                                                                                       ggATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGCCGGGTGAAATTCCCTCTGCCCGCTTACCGATGAGCGATGTGc                         <  1:814251/149‑1 (MQ=255)                                                                                                                       ggATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGCCGGGTGAAATTCCCTCTGCCCGCTTACCGATGAGCGATGTGCGt                       >  2:38775/1‑151 (MQ=255)                                                                                                                       ggATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGCCGGGTGAAATTCCCTCTGCCCGCTTACCGATGAGCGATGTGCGt                       <  1:396092/151‑1 (MQ=255)                                                                                                                        gATCCGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGCCGGGTGAAATTCCCTCTGCCCGCTTACCGATGAGCGATGTGCGtt                      >  2:188813/1‑151 (MQ=255)                                                                                                                           ccGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGCCGGGTGAAATTCCCTCTGCCCGCTTACCGATGAGCGATGTGCGttttt                   <  2:86235/151‑1 (MQ=255)                                                                                                                           ccGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGCCGGGTGAAATTCCCTCTGCCCGCTTACCGATGAGCGATGTGCGttttt                   <  1:765898/151‑1 (MQ=255)                                                                                                                           ccGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGCCGGGTGAAATTCCCTCTGCCCGCTTACCGATGAGCGATGTGCGtttt                    <  1:705780/150‑1 (MQ=255)                                                                                                                           ccGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGCCGGGTGAAATTCCCTCTGCCCGCTTACCGATGAGCGATGTGCGtttt                    <  1:659824/150‑1 (MQ=255)                                                                                                                            cGGCAATCTGGCTGGAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGCCGGGTGAAATTCCCTCTGCCCGCTTACCGATGAGCGATGTGCGttttt                   >  1:817456/1‑150 (MQ=255)                                                                                                                                aaTCTGGCTGGAAGAACTGACTGCCCTTCCCGATGCGACCCGCAAACGCGCACAGGCGCTGGTCGATCAAGGCAGCACCATTGAGTTGTTCTGTGCGCCGGGTTAAATTCCCTCTGCCCGCTTACCGATGAGCGATGTGGGTTTTTAtt                <  2:696074/149‑1 (MQ=255)                                                                                                                                     ggctggAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGCCGGGTGAAATTCCCTCTGCCCGCTTACCGATGAGCGATGTGCGTTTTTATTCCCgca          <  2:629383/150‑1 (MQ=255)                                                                                                                                        tggAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGCCGGGTGAAATTCCCTCTGCCCGCTTACCGATGAGCGATGTGCGTTTTTATTCCCgcagc        >  2:131913/1‑149 (MQ=255)                                                                                                                                         ggAAGAACTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGCCGGGTGAAATTCCCTCTGCCCGCTTACCGATGAGCGATGTGCGTTTTTATTCCCGCAGCAGt     <  2:185540/151‑1 (MQ=255)                                                                                                                                           aagaaCTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGCCGGGTGAAATTCCCTCTGCCCGCTTACCGATGAGCGATGTGCGTTTTTATTCCCGCAGCAGTAt   >  2:182691/1‑151 (MQ=255)                                                                                                                                            agaaCTGACTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGCCGGGTGAAATTCCCTCTGCCCGCTTACCGATGAGCGATGTGCGTTTTTATTCCCGCAGCAGTAtt  >  1:495706/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                TGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACTCAGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGAACTGGCTACCCTTCCCGATGCTACCCGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGCCGGGTGAAATTCCCTCTGCCCGCTTACCGATGAGCGATGTGCGTTTTTATTCCCGCAGCAGTATT  >  NC_000913/2209293‑2209582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑