Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,274,782 T→C F202L (TTC→CTC)  yejB → microcin C ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,274,7820TC98.6% 247.1 / ‑3.8 70F202L (TTC→CTC) yejBmicrocin C ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (32/37);  total (33/37)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01

TCCGTTAGGCATTCGCAAAGCTGTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGTTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTGCGGCGCTGACCATGCTGACAAAAAACTC  >  NC_000913/2274634‑2274927
                                                                                                                                                    |                                                                                                                                                 
tCCGTTAGGCATTCGCAAAGCTGTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTc                                                                                                                                                 >  1:699760/1‑151 (MQ=255)
   gTTAGGCATTCGCAAAGCTGTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCct                                                                                                                                              <  2:824148/151‑1 (MQ=255)
      aGGCATTCGCAAAGCTGTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCctct                                                                                                                                            <  2:288722/150‑1 (MQ=255)
      aGGCATTCGCAAAGCTGTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCctc                                                                                                                                             <  2:60270/149‑1 (MQ=255)
      aGGCATTCGCAAAGCTGTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTa                                                                                                                                           <  1:741909/151‑1 (MQ=255)
       ggCATTCGCAAAGCTGTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTAc                                                                                                                                          <  1:203514/151‑1 (MQ=255)
          aTTCGCAAAGCTGTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTAcgcg                                                                                                                                       >  2:1001137/1‑151 (MQ=255)
                 aaGCTGTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGt                                                                                                                                <  2:436616/151‑1 (MQ=255)
                   gCTGTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGttt                                                                                                                              <  1:508717/151‑1 (MQ=255)
                   gCTGTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGttt                                                                                                                              >  2:596986/1‑151 (MQ=255)
                      gTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTcc                                                                                                                            <  1:183847/150‑1 (MQ=255)
                      gTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTc                                                                                                                             <  1:838644/149‑1 (MQ=255)
                        ttATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGc                                                                                                                          <  1:1029116/150‑1 (MQ=255)
                         tataATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCt                                                                                                                         >  2:316961/1‑150 (MQ=255)
                           taATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAAc                                                                                                                      >  1:880467/1‑151 (MQ=255)
                                    ccGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCg                                                                                                             <  2:334550/151‑1 (MQ=255)
                                           gACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCg                                                                                                       <  1:620067/150‑1 (MQ=255)
                                            aCGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTg                                                                                                     >  2:884870/1‑151 (MQ=255)
                                              gTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGt                                                                                                   >  1:925574/1‑151 (MQ=255)
                                                  ggAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATc                                                                                                <  2:774259/150‑1 (MQ=255)
                                                  ggAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATc                                                                                                <  2:811695/150‑1 (MQ=255)
                                                       aGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGaaa                                                                                           >  1:379231/1‑150 (MQ=255)
                                                          gcATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCa                                                                                       <  2:887248/151‑1 (MQ=255)
                                                          gcATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCa                                                                                       <  1:429168/151‑1 (MQ=255)
                                                           cATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCAc                                                                                      <  2:455861/151‑1 (MQ=255)
                                                           cATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCAc                                                                                      >  2:112750/1‑151 (MQ=255)
                                                                atcatcatcGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGAtt                                                                                 >  1:132943/1‑151 (MQ=255)
                                                                   atcatcGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATc                                                                              >  2:190390/1‑151 (MQ=255)
                                                                      atcGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCtgt                                                                           >  1:603623/1‑151 (MQ=255)
                                                                      atcGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCtgt                                                                           <  1:332220/151‑1 (MQ=255)
                                                                        cGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGCTTGTCTTCTTCGCGGGCGGCCGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTgg                                                                         >  2:133270/1‑151 (MQ=255)
                                                                                ccATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCAc                                                                 <  1:1018958/151‑1 (MQ=255)
                                                                                 cATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGACTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACg                                                                >  1:986873/1‑151 (MQ=255)
                                                                                       ggCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCg                                                          >  2:1009576/1‑151 (MQ=255)
                                                                                        gCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCgg                                                         >  2:496601/1‑151 (MQ=255)
                                                                                         ccTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGt                                                        <  2:477520/151‑1 (MQ=255)
                                                                                         ccTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGt                                                        <  2:454283/151‑1 (MQ=255)
                                                                                          cTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTg                                                       >  1:1040150/1‑151 (MQ=255)
                                                                                           tttttGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGc                                                      >  2:511985/1‑151 (MQ=255)
                                                                                           tttttGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGc                                                      >  1:84944/1‑151 (MQ=255)
                                                                                            ttttGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTg                                                       <  2:466270/149‑1 (MQ=255)
                                                                                            ttttGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCt                                                     >  1:956072/1‑151 (MQ=255)
                                                                                            ttttGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCt                                                     >  2:531035/1‑151 (MQ=255)
                                                                                             tttgtttgCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCt                                                     <  1:445019/150‑1 (MQ=255)
                                                                                              ttgtttgCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTgg                                                   >  1:919123/1‑151 (MQ=255)
                                                                                                    gCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACa                                              <  1:743231/150‑1 (MQ=255)
                                                                                                     ccATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAg                                             >  2:669415/1‑150 (MQ=255)
                                                                                                     ccATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGt                                            >  2:732759/1‑151 (MQ=255)
                                                                                                               gATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATtggtgg                                   <  1:471262/150‑1 (MQ=255)
                                                                                                               gATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGc                                  >  2:514686/1‑151 (MQ=255)
                                                                                                                  tGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGTCAGTGATTGGTGGCtt                                <  2:101500/150‑1 (MQ=255)
                                                                                                                   gTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCttt                               <  2:36592/150‑1 (MQ=255)
                                                                                                                   gTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCttt                               <  1:1029765/150‑1 (MQ=255)
                                                                                                                     cttcttCGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTCCCGGTGCTGGCGACAGTGATTGGTGGCTTTgcg                            >  1:520850/1‑151 (MQ=255)
                                                                                                                      ttcttcGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTgcgg                           >  1:449259/1‑151 (MQ=255)
                                                                                                                      ttcttcGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTgcgg                           >  2:775438/1‑151 (MQ=255)
                                                                                                                          tcGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTgcggcg                         <  1:266401/149‑1 (MQ=255)
                                                                                                                          tcGCGGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTgcggcg                         <  2:565536/149‑1 (MQ=255)
                                                                                                                           cgcgGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTGCGgcgc                        <  2:683184/149‑1 (MQ=255)
                                                                                                                           cgcgGGCGGCAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTGCGGCGCTg                      <  2:147642/151‑1 (MQ=255)
                                                                                                                                   gcAGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTGCGGCGCTGACCATGCt              >  1:809834/1‑151 (MQ=255)
                                                                                                                                     aGCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTGCGGCGCTGACCATGCTg             >  1:141381/1‑150 (MQ=255)
                                                                                                                                     aGCGATGTCGTCCTGCTTCCTCTACGGGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTCGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGGGGCGTTGCGGCGCTGGTCATGCGGa            <  2:846769/151‑1 (MQ=255)
                                                                                                                                      gCTATTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTGCGGCGCTGACCATGCTGAc           <  2:497235/151‑1 (MQ=255)
                                                                                                                                        tATTTCGACCTGTTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTGCGGCGCTGACCATGCTGACa          >  1:942550/1‑150 (MQ=255)
                                                                                                                                         aTTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTGCGGCGCTGACCATGCTGACaaa        <  2:774271/151‑1 (MQ=255)
                                                                                                                                         aTTTCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGGATCCGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACCGTGATTGGTGGATTTGCGGCGCTGACCCTGCTGACa          >  2:388007/1‑149 (MQ=255)
                                                                                                                                          tttCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTGCGGCGCTGACCATGCTGACaaa        >  1:296009/1‑150 (MQ=255)
                                                                                                                                            tCGACCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTGCGGCGCTGACCATGCTGACaaaaa      <  1:382439/150‑1 (MQ=255)
                                                                                                                                               aCCTGCTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTGCGGCGCTGACCATGCTGACAAAAAACTc  <  2:173445/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TCCGTTAGGCATTCGCAAAGCTGTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTCGCGGGCGGCAGCTATTTCGACCTGTTCCCTCTACGCGGCCTGGTTTCCGCTAACTTTGATTCGCTGCCGTGGTATCAGAAAATCACCGATTATCTGTGGCATATCACGCTGCCGGTGCTGGCGACAGTGATTGGTGGCTTTGCGGCGCTGACCATGCTGACAAAAAACTC  >  NC_000913/2274634‑2274927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: