Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,337,019	G→A	A801V (GCC→GTC)	gyrA ←	DNA gyrase (type II topoisomerase), subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,337,019	0	G	A	100.0%	219.0 / NA	56	A801V (GCC→GTC)	gyrA	DNA gyrase (type II topoisomerase), subunit A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/30);  total (26/30)

ATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCG  >  NC_000913/2336874‑2337165                                                                                                                                                  |                                                                                                                                                   aTCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATc                                                                                                                                                <  2:936061/150‑1 (MQ=255) aTCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCg                                                                                                                                               >  2:798050/1‑151 (MQ=255)     aGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTg                                                                                                                                            >  2:396749/1‑150 (MQ=255)        tCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGatc                                                                                                                                         >  1:953360/1‑150 (MQ=255)           tCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGatcatc                                                                                                                                      <  2:820208/150‑1 (MQ=255)            ccTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGAtcatca                                                                                                                                     <  1:870018/150‑1 (MQ=255)            ccTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATcatcat                                                                                                                                    <  1:926021/151‑1 (MQ=255)               cGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGAt                                                                                                                                 <  1:282053/151‑1 (MQ=255)               cGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGAt                                                                                                                                 <  1:943801/151‑1 (MQ=255)                gTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGGTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATc                                                                                                                                <  2:984850/151‑1 (MQ=255)                  cAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCt                                                                                                                               <  2:445279/150‑1 (MQ=255)                    aCCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGt                                                                                                                            >  1:488559/1‑151 (MQ=255)                    aCCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGt                                                                                                                            <  1:896282/151‑1 (MQ=255)                               aaCACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCt                                                                                                                 >  1:994322/1‑151 (MQ=255)                               aaCACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCt                                                                                                                 <  1:954063/151‑1 (MQ=255)                                       gCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTAc                                                                                                         >  1:660351/1‑151 (MQ=255)                                         aGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACcg                                                                                                       <  1:505328/151‑1 (MQ=255)                                          gACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACcgc                                                                                                      >  1:935164/1‑151 (MQ=255)                                           aCCCACTACGTTTTCAACTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACcg                                                                                                       <  2:745273/149‑1 (MQ=255)                                             ccACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGcc                                                                                                   >  1:933831/1‑151 (MQ=255)                                              cACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCca                                                                                                  <  2:663091/151‑1 (MQ=255)                                                 tACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCcaac                                                                                                >  2:492271/1‑150 (MQ=255)                                                  aCGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCcaaca                                                                                               <  1:809460/150‑1 (MQ=255)                                                   cGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCcaac                                                                                                <  1:64101/148‑1 (MQ=255)                                                      tttCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTa                                                                                           >  1:473264/1‑150 (MQ=255)                                                        tCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAAc                                                                                        >  1:39359/1‑151 (MQ=255)                                                        tCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAAc                                                                                        >  2:129843/1‑151 (MQ=255)                                                         cATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAAcc                                                                                       >  2:298865/1‑151 (MQ=255)                                                            cTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGtt                                                                                    >  2:442484/1‑151 (MQ=255)                                                                  cAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTc                                                                              >  1:1061389/1‑151 (MQ=255)                                                                  cAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTc                                                                              <  1:1051787/151‑1 (MQ=255)                                                                    gTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCgg                                                                            <  1:212243/151‑1 (MQ=255)                                                                      aCGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTa                                                                          <  1:545281/151‑1 (MQ=255)                                                                         gATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAAcc                                                                       >  1:631478/1‑151 (MQ=255)                                                                            gAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTg                                                                    >  2:926435/1‑151 (MQ=255)                                                                             aGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGa                                                                   <  2:777784/151‑1 (MQ=255)                                                                              ggATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGAt                                                                  <  1:442510/151‑1 (MQ=255)                                                                               gATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATg                                                                 >  2:467311/1‑151 (MQ=255)                                                                               gATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATg                                                                 >  2:562780/1‑151 (MQ=255)                                                                                 tCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATgg                                                                >  1:165077/1‑150 (MQ=255)                                                                                  cACGCCCTGGGTGTTACGGCCCCCGATGCTAATTTCCAAAACGCGAGTCCGTCCCAGCTTACCGACATCGGTGTTCATAATGATCTGGTCGCAGCCATCTCCCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACTTTGATgg                                                                <  2:324762/149‑1 (MQ=255)                                                                                  cACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGgag                                                              <  1:18774/151‑1 (MQ=255)                                                                                     gCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGAt                                                            >  1:484647/1‑150 (MQ=255)                                                                                          gggTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTTCGTTCGGTAACCTTGATGGAGATAAcccc                                                      <  1:440556/151‑1 (MQ=255)                                                                                           ggTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCt                                                     <  1:629085/151‑1 (MQ=255)                                                                                               ttACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTtcg                                                 >  1:830705/1‑151 (MQ=255)                                                                                                     cccACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCAc                                           >  1:911183/1‑151 (MQ=255)                                                                                                      ccACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCAcg                                          <  1:436621/151‑1 (MQ=255)                                                                                                      ccACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCAcg                                          >  2:989119/1‑151 (MQ=255)                                                                                                       cccGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATTGAGATAACCCCTTTCGTCGCAcgc                                         <  2:1069375/149‑1 (MQ=255)                                                                                                        aCGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCAcgcg                                        <  1:989171/151‑1 (MQ=255)                                                                                                                    tCCGAAACGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTggg                             <  1:217780/150‑1 (MQ=255)                                                                                                                        aaaCGCGAGTACGTACCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTc                        <  1:548887/151‑1 (MQ=255)                                                                                                                                     tacCAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGt            >  1:432985/1‑150 (MQ=255)                                                                                                                                       ccAGCGTACCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACg         <  1:115742/151‑1 (MQ=255)                                                                                                                                              aCCGACATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCg  >  2:846474/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                                   ATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCG  >  NC_000913/2336874‑2337165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑