Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,344,828	T→C	intergenic (‑659/‑37)	yfaL ← / → nrdA	adhesin/ribonucleoside‑diphosphate reductase 1, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,344,828	0	T	C	100.0%	214.7 / NA	55	intergenic (‑659/‑37)	yfaL/nrdA	adhesin/ribonucleoside‑diphosphate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/25);  total (30/25)

AAAGCCACTTTTTCCTTCCTGAGTTATCCACAAAGTTATGCACTTGCAAGAGGGTCATTTTCACACTATCTTGCAGTGAATCCCAAACATACCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACTATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATCTGCTGGTGACAAAGCGCGACGGTAGCACAGAGCGCATCAATCTCGACAAAATCCATCGCGTTCTGGATTGGGCGGCAGAAGGACTGCATAACGTTTCG  >  NC_000913/2344680‑2344975                                                                                                                                                     |                                                                                                                                                    aaaGCCACTTTTTCCTTCCTGAGTTATCCACAAAGTTATGCACTTGCAAGAGGGTCATTTTCACACTATCTTGCAGTGAATCCCAAACATACCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCAt                                                                                                                                                   >  1:390743/1‑151 (MQ=255)  aaGCCACTTTTTCCTTCCTGAGTTATCCACAAAGTTATGCACTTGCAAGAGGGTCATTTTCACACTATCTTGCAGTGAATCCCAAACATACCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCAtt                                                                                                                                                  >  1:644133/1‑151 (MQ=255)   aGCCACTTTTTCCTTCCTGAGTTATCCACAAAGTTATGCACTTGCAAGAGGGTCATTTTCACACTATCTTGCAGTGAATCCCAAACATACCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTg                                                                                                                                                 >  1:747053/1‑151 (MQ=255)      cACTTTTTCCTTCCTGAGTTATCCACAAAGTTATGCACTTGCAAGAGGGTCATTTTCACACTATCTTGCAGTGAATCCCAAACATACCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCgg                                                                                                                                              >  1:1047224/1‑151 (MQ=255)              ccttcctGAGTTATCCACAAAGTTATGCACTTGCAAGAGGGTCATTTTCACACTATCTTGCAGTGAATCCCAAACATACCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAAtt                                                                                                                                      <  2:552282/151‑1 (MQ=255)              ccttcctGAGTTATCCACAAAGTTATGCACTTGCAAGAGGGTCATTTTCACACTATCTTGCAGTGAATCCCAAACATACCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAAtt                                                                                                                                      <  1:556519/151‑1 (MQ=255)              ccttcctGAGTTATCCACAAAGTTATGCACTTGCAAGAGGGTCATTTTCACACTATCTTGCAGTGAATCCCAAACATACCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGCATTCTGCATGAAACCATTGCGGAAAGAAtt                                                                                                                                      >  2:605922/1‑151 (MQ=255)                       gTTATCCACAAAGTTATGCACTTGCAAGAGGGTCATTTTCACTCTATCTTGCAGTGAATCCCAAACATACCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACa                                                                                                                             <  2:639738/151‑1 (MQ=255)                         tATCCACAAAGTTATGCACTTGCAAGAGGGTCATTTTCACACTATCTTGCAGTGAATCCCAAACATACCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAgg                                                                                                                           >  1:81613/1‑151 (MQ=255)                          aTCCACAAAGTTATGCACTTGCAAGAGGGTCATTTTCACACTATCTTGCAGTGAATCCCAAACATACCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAgg                                                                                                                           <  1:245114/150‑1 (MQ=255)                          aTCCACAAAGTTATGCACTTGCAAGAGGGTCATTTTCACACTATCTTGCAGTGAATCCCAAACATACCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAgg                                                                                                                           <  1:420547/150‑1 (MQ=255)                           tCCACAAAGTTATGCACTTGCAAGAGGGTCATTTTCACACTATCTTGCAGTGAATCCCAAACATACCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGt                                                                                                                          >  2:124533/1‑150 (MQ=255)                           tCCACAAAGTTATGCACTTGCAAGAGGGTCATTTTCACACTATCTTGCAGTGAATCCCAAACATACCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGt                                                                                                                          >  2:1039100/1‑150 (MQ=255)                                 aaGTTATGCACTTGCAAGAGGGTCATTTTCACACTATCTTGCAGTGAATCCCAAACATACCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGacat                                                                                                                   >  1:179819/1‑151 (MQ=255)                                 aaGTTATGCACTTGCAAGAGGGTCATTTTCACACTATCTTGCAGTGAATCCCAAACATACCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGacat                                                                                                                   >  2:1067914/1‑151 (MQ=255)                                  aGTTATGCACTTGCAAGAGGGTCATTTTCACACTATCTTGCAGTGAATCCCAAACATACCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGacat                                                                                                                   >  2:849112/1‑150 (MQ=255)                                    ttATGCACTTGCAAGAGGGTCATTTTCACACTATCTTGCAGTGAATCCCAAACATACCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGacatac                                                                                                                 >  2:272781/1‑150 (MQ=255)                                      aTGCACTTGCAAGAGGGTCATTTTCACACTATCTTGCAGTGAATCCCAAACATACCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATg                                                                                                              <  2:215617/151‑1 (MQ=255)                                         cACTTGCAAGAGGGTCATTTTCACACTATCTTGCAGTGAATCCCAAACATACCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAAt                                                                                                           <  1:916448/151‑1 (MQ=255)                                          aCTTGCAAGAGGGTCATTTTCACACTATCTTGCAGTGAATCCCAAACATACCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATc                                                                                                          <  1:434147/151‑1 (MQ=255)                                               cAAGAGGGTCATTTTCACACTATCTTGCAGTGAATCCCAAACATGCCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAAt                                                                                                     <  2:435660/151‑1 (MQ=255)                                               cAAGAGGGTCATTTTCACACTATCTTGCAGTGAATCCCAAACATACCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAAt                                                                                                     <  1:671122/151‑1 (MQ=255)                                                aaGAGGGTCATTTTCACACTATCTTGCAGTGAATCCCAAACATACCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATc                                                                                                    >  1:904909/1‑151 (MQ=255)                                                    gggTCATTTTCACACTATCTTGCAGTGAATCCCAAACATACCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATctgct                                                                                                >  1:496989/1‑151 (MQ=255)                                                    gggTCATTTTCACACTATCTTGCAGTGAATCCCAAACATACCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATctgct                                                                                                <  1:169945/151‑1 (MQ=255)                                                     ggTCATTTTCACACTATCTTGCAGTGAATCCCAAACATTCCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATctgct                                                                                                <  1:566868/150‑1 (MQ=255)                                                     ggTCATTTTCACACTATCTTGCAGTGAATCCCAAACATACCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATctgctg                                                                                               >  1:605976/1‑151 (MQ=255)                                                          ttttCACACTATCTTGCAGTGAATCCCAAACATACCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATCTGCTGGTGAc                                                                                          >  1:748546/1‑151 (MQ=255)                                                                        tGCAGTGAATCCCAAACATACCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATCTGCTGGTGACAAAGCGCGACg                                                                               <  2:574950/148‑1 (MQ=255)                                                                              gAATCCCAAACATACCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATCTGCTGGTGACAAAGCGCGACGGTAGcac                                                                        >  2:3123/1‑149 (MQ=255)                                                                                 tCCCAAACATACCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATCTGCTGGTGACAAAGCGCGACGGTAGCACagag                                                                    >  2:142746/1‑150 (MQ=255)                                                                                  cccAAACATACCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATCTGCTGGTGACAAAGCGCGACGGTAGCACAGAgcg                                                                  >  1:607048/1‑151 (MQ=255)                                                                                             ccccTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATCTGCTGGTGACAAAGCGCGACGGTAGCACAGAGCGCATCAATCTCg                                                       >  1:428471/1‑151 (MQ=255)                                                                                             ccccTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATCTGCTGGTGACAAAGCGCGACGGTAGCACAGAGCGCATCAATCTCg                                                       >  1:533685/1‑151 (MQ=255)                                                                                              cccTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATCTGCTGGTGACAAAGCGCGACGGTAGCACAGAGCGCATCAATCTCGa                                                      <  1:383999/151‑1 (MQ=255)                                                                                                cTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATCTGCTGGTGACAAAGCGCGACGGTAGCACAGAGCGCATCAATCTCGAc                                                     >  2:109994/1‑150 (MQ=255)                                                                                                    atataGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATCTGCTGGTGACAAAGCGCGACGGTAGCACAGAGCGCATCAATCTCGACaaaa                                                 <  1:711284/150‑1 (MQ=255)                                                                                                      ttaGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATCTGCTGGTGACAAAGCGCGACGGTAGCACAGAGCGCATCAATCTCGACAAAATcc                                              >  1:484179/2‑151 (MQ=255)                                                                                                      ataGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATCTGCTGGTGACAAAGCGCGACGGTAGCACAGAGCGCATCAATCTCGACAAAATc                                               <  1:1039155/150‑1 (MQ=255)                                                                                                           gtTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATCTGCTGGTGACAAAGCGCGACGGTAGCACAGAGCGCATCAATCTCGACAAAATCCATcg                                          <  2:81612/150‑1 (MQ=255)                                                                                                                aaGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATCTGCTGGTGACAAAGCGCGACGGTAGCACAGAGCGCATCAATCTCGACAAAATCCATCGCGt                                       <  2:211236/148‑1 (MQ=255)                                                                                                                 agcagcTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATCTGCTGGTGACAAAGCGCGACGGTAGCACAGAGCGCATCAATCTCGACAAAATCCATCGCGTTCTg                                   <  1:755701/151‑1 (MQ=255)                                                                                                                      cTTCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATCTGCTGGTGACAAAGCGCGACGGTAGCACAGAGCGCATCAATCTCGACAAAATCCATCGCGTTCTGGATTg                              >  2:577125/1‑151 (MQ=255)                                                                                                                       ttCCCGTACTACAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATCTGCTGGTGACAAAGCGCGACGGTAGCACAGAGCGCATCAATCTCGACAAAATCCATCGCGTTCTGGATTgg                             >  2:177435/1‑151 (MQ=255)                                                                                                                                 acAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATCTGCTGGTGACAAAGCGCGACGGTAGCACAGAGCGCATCAATCTCGACAAAATCCATCGCGTTCTGGATTGGGCGGCAGaa                    <  1:671606/150‑1 (MQ=255)                                                                                                                                 acAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATCTGCTGGTGACAAAGCGCGACGGTAGCACAGAGCGCATCAATCTCGACAAAATCCATCGCGTTCTGGATTGGGCGGCAGaa                    <  1:164118/150‑1 (MQ=255)                                                                                                                                 acAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATCTGCTGGTGACAAAGCGCGACGGTAGCACAGAGCGCATCAATCTCGACAAAATCCATCGCGTTCTGGATTGGGCGGCAGa                     >  2:360743/1‑149 (MQ=255)                                                                                                                                 acAGGTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATCTGCTGGTGACAAAGCGCGACGGTAGCACAGAGCGCATCAATCTCGACAAAATCCATCGCGTTCTGGATTGGGCGGCAGa                     <  2:607015/149‑1 (MQ=255)                                                                                                                                    ggTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATCTGCTGGTGACAAAGCGCGACGGTAGCACAGAGCGCATc                                                              <  2:539798/105‑1 (MQ=255)                                                                                                                                    ggTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATCTGCTGGTGACAAAGCGCGACGGTAGCACAGAGCGCATc                                                              >  1:539829/1‑105 (MQ=255)                                                                                                                                     gTAGTCTGCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATCTGCTGGTGACAAAGCGCGACGGTAGCACAGAGCGCATCAATCTCGACAAAATCCATCGCGTTCTGGATTGGGCGGCAGAAGGACt               <  2:517290/151‑1 (MQ=255)                                                                                                                                            gCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATCTGCTGGTGACAAAGCGCGACGGTAGCACAGAGCGCATCAATCTCGACAAAATCCATCGCGTTCTGGATTGGGCGGCAGAAGGACTGCATa          <  1:577157/149‑1 (MQ=255)                                                                                                                                            gCATGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATCTGCTGGTGACAAAGCGCGACGGTAGCACAGAGCGCATCAATCTCGACAAAATCCATCGCGTTCTGGATTGGGCGGCAGAAGGACTGCATAAc        >  1:387880/1‑151 (MQ=255)                                                                                                                                               tGAAACCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATCTGCTGGTGACAAAGCGCGACGGTAGCACAGAGCGCATCAATCTCGACAAAATCCATCGCGTTCTGGATTGGGCGGCAGAAGGACTGCATAACGtt     >  2:56523/1‑151 (MQ=255)                                                                                                                                                  aaCCATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATCTGCTGGTGACAAAGCGCGACGGTAGCACAGAGCGCATCAATCTCGACAAAATCCATCGCGTTCTGGATTGGGCGGCAGAAGGACTGCATAACGTTTCg  >  1:115501/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                    AAAGCCACTTTTTCCTTCCTGAGTTATCCACAAAGTTATGCACTTGCAAGAGGGTCATTTTCACACTATCTTGCAGTGAATCCCAAACATACCCCCTATATATAGTGTTCTAAGCAGCTTCCCGTACTACAGGTAGTCTGCATGAAACTATTGCGGAAAGAATTCCAAAAACAGGTACGACATACATGAATCAGAATCTGCTGGTGACAAAGCGCGACGGTAGCACAGAGCGCATCAATCTCGACAAAATCCATCGCGTTCTGGATTGGGCGGCAGAAGGACTGCATAACGTTTCG  >  NC_000913/2344680‑2344975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑