Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,353,790	G→A	A382T (GCC→ACC)	glpA →	anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, large FAD/NAD(P)‑binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,353,790	0	G	A	100.0%	252.9 / NA	67	A382T (GCC→ACC)	glpA	anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, large FAD/NAD(P)‑binding subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/29);  total (38/29)

ATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCT  >  NC_000913/2353643‑2353929                                                                                                                                                    |                                                                                                                                            aTCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCg                                                                                                                                          >  1:383298/1‑151 (MQ=255)   cGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCCCCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGGAAACTGGGCAACACGCGCCCCTGTACGACTACCGAt                                                                                                                                        <  2:121568/151‑1 (MQ=255)       ctgctCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTgg                                                                                                                                    >  2:259758/1‑151 (MQ=255)        tgctCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGc                                                                                                                                   >  1:222092/1‑151 (MQ=255)        tgctCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGAACTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGc                                                                                                                                   >  2:604418/1‑151 (MQ=255)         gctCGACCATTCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACATGCGCCCCTGGACGACTACCGATCTgg                                                                                                                                    >  2:87777/1‑149 (MQ=255)           tCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACt                                                                                                                                >  1:309049/1‑151 (MQ=255)           tCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACt                                                                                                                                >  2:543504/1‑151 (MQ=255)               ccATGATGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATAGGCTGATGGCTGAATGGGCTAACGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCt                                                                                                                            >  2:689338/1‑151 (MQ=255)                 aTGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTgg                                                                                                                          <  1:195386/151‑1 (MQ=255)                   gCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGtt                                                                                                                        >  1:858967/1‑151 (MQ=255)                         cgcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGGATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTcaca                                                                                                                    >  2:437400/1‑149 (MQ=255)                          gcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTcaca                                                                                                                    >  1:68749/1‑148 (MQ=255)                             aTGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAAcc                                                                                                              >  2:861926/1‑151 (MQ=255)                              tGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAAccc                                                                                                             >  1:481127/1‑151 (MQ=255)                               ggTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCg                                                                                                            >  2:421480/1‑151 (MQ=255)                               ggTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCg                                                                                                            >  2:1054981/1‑151 (MQ=255)                                    ggACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGa                                                                                                        <  1:71746/150‑1 (MQ=255)                                      aCGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGt                                                                                                     >  1:318136/1‑151 (MQ=255)                                           tttATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCt                                                                                                >  2:269554/1‑151 (MQ=255)                                              atcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTg                                                                                              <  2:474177/150‑1 (MQ=255)                                              atcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTg                                                                                              <  1:35439/150‑1 (MQ=255)                                                  ccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTaa                                                                                         <  1:599041/151‑1 (MQ=255)                                                      caccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCGGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGt                                                                                      <  2:124575/150‑1 (MQ=255)                                                        ccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAGCTGGGCAACACGCGCCCCTGTACGGCTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCa                                                                                    <  2:25551/150‑1 (MQ=255)                                                        ccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCAt                                                                                   >  1:715138/1‑151 (MQ=255)                                                         cGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGTCTACCGATCTGGCACTGCCTGGTGCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCAtc                                                                                  <  2:954923/151‑1 (MQ=255)                                                         cGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCAtc                                                                                  >  1:360969/1‑151 (MQ=255)                                                         cGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCAtc                                                                                  >  1:682242/1‑151 (MQ=255)                                                            tGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTcc                                                                               >  1:933065/1‑151 (MQ=255)                                                              gCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCcct                                                                             <  2:397169/151‑1 (MQ=255)                                                               cAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCcctg                                                                            <  2:87661/151‑1 (MQ=255)                                                               cAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCcctg                                                                            >  1:262015/1‑151 (MQ=255)                                                               cAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCcctg                                                                            <  1:339635/151‑1 (MQ=255)                                                                aaaCTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCcctgc                                                                           >  2:242968/1‑151 (MQ=255)                                                                 aaCTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCcctgcc                                                                          >  1:371523/1‑151 (MQ=255)                                                                  aCTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCcctgcct                                                                         >  1:117697/1‑151 (MQ=255)                                                                  aCTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCcctgcc                                                                          >  2:789281/1‑150 (MQ=255)                                                                     gatgaCCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCcctgcctg                                                                        <  2:1014798/149‑1 (MQ=255)                                                                      atgaCCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGccc                                                                     >  1:644461/1‑151 (MQ=255)                                                                          ccTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCt                                                                 <  1:923468/151‑1 (MQ=255)                                                                          ccTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCt                                                                 <  2:778331/151‑1 (MQ=255)                                                                           cTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTg                                                                >  1:685250/1‑151 (MQ=255)                                                                            tATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTgc                                                               >  2:802382/1‑151 (MQ=255)                                                                             aTCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTgc                                                               <  1:76018/150‑1 (MQ=255)                                                                              tCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTgcgc                                                             >  1:976089/1‑151 (MQ=255)                                                                              tCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTgcgc                                                             >  1:394083/1‑151 (MQ=255)                                                                               cGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGcgcg                                                            >  2:507383/1‑151 (MQ=255)                                                                                        gCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCgg                                                   <  2:228583/151‑1 (MQ=255)                                                                                           gAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGtt                                                 <  2:737565/150‑1 (MQ=255)                                                                                            aaTGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGttt                                                >  2:858896/1‑150 (MQ=255)                                                                                                    aCCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATg                                       <  1:300474/151‑1 (MQ=255)                                                                                                     ccGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTTACAAGAACCCGCTGAAGTTTCCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATgg                                      <  2:460799/151‑1 (MQ=255)                                                                                                      cGACGCGGTATGACGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGc                                     >  1:270799/1‑151 (MQ=255)                                                                                                       gACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGca                                    <  2:481288/151‑2 (MQ=255)                                                                                                       gACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCg                                    <  1:1048884/151‑1 (MQ=255)                                                                                                             gTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGc                               >  1:404774/1‑150 (MQ=255)                                                                                                               aTGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCAc                             <  1:507410/150‑1 (MQ=255)                                                                                                                      aaaCTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACgcctgcct                     >  1:585745/1‑151 (MQ=255)                                                                                                                        aCTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTgg                   >  1:923231/1‑151 (MQ=255)                                                                                                                           gggCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTg                <  1:621100/151‑1 (MQ=255)                                                                                                                              cAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGc             <  1:409252/151‑1 (MQ=255)                                                                                                                                acacGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGa           <  2:731628/151‑1 (MQ=255)                                                                                                                                      gcCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCg     >  1:398518/1‑151 (MQ=255)                                                                                                                                      gcCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCg     <  1:851506/151‑1 (MQ=255)                                                                                                                                         ccTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCGGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGcc      <  2:673191/147‑1 (MQ=255)                                                                                                                                           tGTTCGACTACCGATCTGGCACTGCCTGTTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCt  <  2:562474/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                            ATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCT  >  NC_000913/2353643‑2353929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑