Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,421,732	A→G	T9A (ACA→GCA)	yfcH →	putative NAD‑dependent nucleotide‑sugar epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,421,732	0	A	G	100.0%	169.8 / NA	46	T9A (ACA→GCA)	yfcH	putative NAD‑dependent nucleotide‑sugar epimerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/25);  total (21/25)

CGTGAACATCACTGGGTGACGTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGACAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTC  >  NC_000913/2421586‑2421878                                                                                                                                                   |                                                                                                                                                   cGTGAACATCACTGGGTGACGTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAgg                                                                                                                                                <  1:606426/151‑1 (MQ=255)     aaCATCACTGGGTGACGTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGAtt                                                                                                                                             >  2:1020940/1‑150 (MQ=255)      aCATCACTGGGTGACGTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTaa                                                                                                                                           <  2:1004317/151‑1 (MQ=255)      aCATCACTGGGTGACGTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTaa                                                                                                                                           <  1:511972/151‑1 (MQ=255)       cATCACTGGGTGACGTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAAt                                                                                                                                          <  1:171282/151‑1 (MQ=255)       cATCACTGGGTGACGTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAAt                                                                                                                                          >  1:496669/1‑151 (MQ=255)          cACTGGGTGACGTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTgg                                                                                                                                       <  1:342727/151‑1 (MQ=255)               ggTGACGTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGcc                                                                                                                                  <  1:1062326/151‑1 (MQ=255)                gttaCGTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCa                                                                                                                                 <  2:308029/148‑1 (MQ=255)                gTGACGTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCa                                                                                                                                 <  2:1045050/151‑1 (MQ=255)                   aCGTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCAttt                                                                                                                              <  2:219575/151‑1 (MQ=255)                       aTGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCCCCGGAGGGGCAGGATTAATTGGTCGCCATTTGAtt                                                                                                                          <  1:556048/151‑1 (MQ=255)                           tGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCcgc                                                                                                                      >  1:684669/1‑151 (MQ=255)                              aGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGtt                                                                                                                   >  2:814827/1‑151 (MQ=255)                              aGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGtt                                                                                                                   <  1:727320/151‑1 (MQ=255)                                         aTAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTg                                                                                                        >  1:95831/1‑151 (MQ=255)                                            aaCTGCACGCGCTGCGGTACGCCGATTCGGTATACATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGTCTGCATTAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGTCTTTTGATTCCGCGTTTGCTGGAGCTgg                                                                                                       <  2:797856/149‑1 (MQ=255)                                              cTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGcc                                                                                                    >  1:725357/1‑150 (MQ=255)                                                   cgcgCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCaaa                                                                                              <  1:831480/151‑1 (MQ=255)                                                   cgcgCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCaa                                                                                               >  2:993154/1‑150 (MQ=255)                                                      tctgcgctACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAAtt                                                                                            >  2:38651/2‑150 (MQ=255)                                                        tgcgctACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACg                                                                                         >  2:641774/1‑151 (MQ=255)                                                         gcgctACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACg                                                                                         <  1:643572/150‑1 (MQ=255)                                                               cGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTg                                                                                   <  1:704021/150‑1 (MQ=255)                                                               cGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGa                                                                                  <  2:635319/151‑1 (MQ=255)                                                                     ttCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGt                                                                             >  2:497938/1‑150 (MQ=255)                                                                                      tAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGt                                                           >  2:281404/1‑151 (MQ=255)                                                                                                gCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCg                                                 <  1:226233/151‑1 (MQ=255)                                                                                                      aTCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTc                                           <  1:508491/151‑1 (MQ=255)                                                                                                          ccATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCggg                                        >  2:690763/1‑150 (MQ=255)                                                                                                            atatCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGa                                     <  1:982672/151‑1 (MQ=255)                                                                                                              atCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGac                                    >  1:847129/1‑150 (MQ=255)                                                                                                               tCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGacac                                  >  1:648084/1‑151 (MQ=255)                                                                                                                cGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACt                                 >  2:237012/1‑151 (MQ=255)                                                                                                                     ggCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGc                            >  2:440438/1‑151 (MQ=255)                                                                                                                        tGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTc                                           <  2:429019/133‑1 (MQ=255)                                                                                                                        tGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTc                                           >  1:429045/1‑133 (MQ=255)                                                                                                                          cATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAggg                       <  1:273579/151‑1 (MQ=255)                                                                                                                           aTGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGc                      >  1:927363/1‑151 (MQ=255)                                                                                                                           aTGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGc                      <  2:965350/151‑1 (MQ=255)                                                                                                                            tGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCt                     >  1:213969/1‑151 (MQ=255)                                                                                                                                  tAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCg                >  2:723200/1‑150 (MQ=255)                                                                                                                                   aGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGa               <  1:709988/150‑1 (MQ=255)                                                                                                                                   aGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGAt              <  2:903462/151‑1 (MQ=255)                                                                                                                                   aGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGAt              <  1:799767/151‑1 (MQ=255)                                                                                                                                        tCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCaa           >  1:202366/1‑149 (MQ=255)                                                                                                                                                ggggCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTc  <  2:103059/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CGTGAACATCACTGGGTGACGTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGACAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACCTC  >  NC_000913/2421586‑2421878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑