Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,548,346	G→A	G82D (GGC→GAC)	yfeW →	penicillin binding protein PBP4B; weak DD‑carboxypeptidase activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,548,346	0	G	A	100.0%	229.9 / NA	60	G82D (GGC→GAC)	yfeW	penicillin binding protein PBP4B; weak DD‑carboxypeptidase activity
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/26);  total (34/26)

ACGTCGAACGGCTTAACCAGATGGATCGCTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGGCAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACGATCGGATTG  >  NC_000913/2548208‑2548486                                                                                                                                           |                                                                                                                                             ccGTCGAACGGCTTAACCAGATGGATCGCTGGATTAGCCAGCACGTTGCTGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGa                                                                                                                                   >  2:339116/2‑150 (MQ=255)       caCGGCTTAAGCAGATGGTTCGCTGGATTAGCCAGCAAGTTGCTGTCGGTTATCCCAGCGGAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGa                                                                                                                               >  2:237169/2‑148 (MQ=255)          ggCTTAACCAGATGGATCGCTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGcc                                                                                                                         >  2:418485/1‑151 (MQ=255)               aaCCAGATGGATCGCTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGTGTGTTGATGGAACAGCCTGTCa                                                                                                                    <  2:825777/151‑1 (MQ=255)                aCCAGATGGATCGCTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGGACGATGACAGCGTGTTGATGGAACAGCCTGTCa                                                                                                                    >  2:298218/1‑150 (MQ=255)                 ccAGATGGATCGCTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCaa                                                                                                                   >  1:830982/1‑150 (MQ=255)                      tGGATCGCTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGccac                                                                                                             >  2:638320/1‑151 (MQ=255)                           cGCTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCg                                                                                                        >  1:852896/1‑151 (MQ=255)                               gtaTTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGa                                                                                                     <  2:627649/148‑1 (MQ=255)                                     gCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTa                                                                                              <  1:286542/151‑1 (MQ=255)                                       cAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATg                                                                                            >  2:522539/1‑151 (MQ=255)                                        aGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATg                                                                                            >  1:610678/1‑150 (MQ=255)                                        aGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATg                                                                                            >  2:214977/1‑150 (MQ=255)                                            aGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTg                                                                                       <  2:290333/151‑1 (MQ=255)                                                  tGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTc                                                                                  >  1:278049/1‑150 (MQ=255)                                                     cGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCaaa                                                                               <  2:46751/150‑1 (MQ=255)                                                     cGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAac                                                                              >  2:1015539/1‑151 (MQ=255)                                                                gCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGt                                                                    >  1:616189/1‑150 (MQ=255)                                                                gCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTa                                                                   <  1:768303/151‑1 (MQ=255)                                                                 cGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTa                                                                   <  2:123108/150‑1 (MQ=255)                                                                  gTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTAc                                                                  <  2:407678/150‑1 (MQ=255)                                                                   tAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGc                                                                >  1:883174/1‑151 (MQ=255)                                                                     aaCCTGCTGATCATTAAAGATAATCAGATGGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCa                                                              <  2:402697/151‑1 (MQ=255)                                                                          gctgATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGa                                                           <  2:152518/149‑1 (MQ=255)                                                                           ctgATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACt                                                        >  2:113467/1‑151 (MQ=255)                                                                             gATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTc                                                      <  2:116701/151‑1 (MQ=255)                                                                                cATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGc                                                    <  1:660426/150‑1 (MQ=255)                                                                                   tAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTg                                                >  2:483027/1‑151 (MQ=255)                                                                                   tAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACATGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTg                                                >  2:452004/1‑151 (MQ=255)                                                                                         tAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCaaa                                            >  2:861436/1‑149 (MQ=255)                                                                                         tAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGGGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCaaa                                            >  1:107628/1‑149 (MQ=255)                                                                                           aTCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCt                                        >  2:90932/1‑151 (MQ=255)                                                                                            tCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCtg                                       >  1:184254/1‑151 (MQ=255)                                                                                             cAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCtg                                       <  2:883127/150‑1 (MQ=255)                                                                                              aGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCtgat                                     >  1:786485/1‑151 (MQ=255)                                                                                               gATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCtgatg                                    >  1:416176/1‑151 (MQ=255)                                                                                                   gtgtATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGc                                 <  2:587662/150‑1 (MQ=255)                                                                                                   gtgtATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCg                                <  1:858902/151‑1 (MQ=255)                                                                                                      tATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGa                               >  2:1026140/1‑149 (MQ=255)                                                                                                       aTCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAgg                            >  2:15311/1‑151 (MQ=255)                                                                                                       aTCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAgg                            <  1:215002/151‑1 (MQ=255)                                                                                                       aTCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAgg                            >  1:488881/1‑151 (MQ=255)                                                                                                         cGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAgg                            >  2:520437/1‑149 (MQ=255)                                                                                                         cGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGc                           >  1:608944/1‑150 (MQ=255)                                                                                                          gCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCaa                         <  1:289614/151‑1 (MQ=255)                                                                                                            aaaGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAAc                       >  2:248023/1‑151 (MQ=255)                                                                                                             aaGCCTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACt                      >  1:794217/1‑151 (MQ=255)                                                                                                                ccTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCa                   <  2:479163/151‑1 (MQ=255)                                                                                                                 cTGGGGAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCAt                  >  2:1059648/1‑151 (MQ=255)                                                                                                                   ggggAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATc                 <  1:33562/150‑1 (MQ=255)                                                                                                                     ggAGCGGCGAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTg              >  1:184959/1‑151 (MQ=255)                                                                                                                        gcggcgAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACg           <  1:519031/151‑1 (MQ=255)                                                                                                                         cggcgAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACGa          <  2:1004439/151‑1 (MQ=255)                                                                                                                           gcgAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACGATc        >  2:986534/1‑151 (MQ=255)                                                                                                                            cgAAAAAGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACGATCg       >  2:70267/1‑151 (MQ=255)                                                                                                                            cgAAAAAGTACGATGACAGCGTGTTGATGCAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACGATc        <  2:525273/150‑1 (MQ=255)                                                                                                                              aaaaaGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACGATCGGa     <  2:379879/151‑1 (MQ=255)                                                                                                                                aaaGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACGATCGGAtt   <  1:662610/151‑1 (MQ=255)                                                                                                                                aaaGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACGATCGGAtt   <  2:1062196/151‑1 (MQ=255)                                                                                                                                 aaGTACGATGACAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACGATCGGATTg  <  1:315627/151‑1 (MQ=255)                                                                                                                                           |                                                                                                                                             ACGTCGAACGGCTTAACCAGATGGATCGCTGGATTAGCCAGCAAGTTGATGTCGGTTATCCCAGCGTAAACCTGCTGATCATTAAAGATAATCAGATTGTGTATCGCAAAGCCTGGGGAGCGGCGAAAAAGTACGATGGCAGCGTGTTGATGGAACAGCCTGTCAAAGCCACCACCGGGACGCTGTATGACCTGGCCTCAAACACCAAAATGTACGCCACGAACTTCGCCCTGCAAAAGCTGATGAGCGAAGGCAAACTGCATCCTGACGATCGGATTG  >  NC_000913/2548208‑2548486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑