Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,584,258	T→C	Y423C (TAC→TGC)	aegA ←	putative oxidoreductase, FeS binding subunit/NAD/FAD‑binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,584,258	0	T	C	100.0%	235.7 / NA	62	Y423C (TAC→TGC)	aegA	putative oxidoreductase, FeS binding subunit/NAD/FAD‑binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/32);  total (30/32)

TTAAGTCCGGCGGTATTGATAAACGGCTCTTCCGGTAGCTCTTCGAGCCCCATCACCTGTTTAGTGTTGGCAATGAGGAACGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGTAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAG  >  NC_000913/2584117‑2584403                                                                                                                                              |                                                                                                                                                  ttAAGTCCGGCGGTATTGATAAACGGCTCTTCCGGTAGCTCTTCGAGCCCCATCACCTGTTTAGTGTTGGCAATGAGGAACGGCAGCGTGTCCTAAACGCCCGGCGCATCTTCAGTGGGTAAACCCGCTTTTATGGAACGGCAAGTGCCt                                                                                                                                           >  2:130460/1‑150 (MQ=255)   aaGTCCGGCGGTATTGATAAACGGCTCTTCCGGTAGCTCTTCGAGCCCCATCACCTGTTTAGTGTTGGCAATGAGGAACGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACg                                                                                                                                        <  2:958792/151‑1 (MQ=255)     gTCCGGCGGTATTGATAAACGGCTCTTCCGGTAGCTCTTCGAGCCCCATCACCTGTTTAGTGTTGGCAATGAGGAACGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGcc                                                                                                                                      >  2:437812/1‑151 (MQ=255)        cggcggTATTGATAAACGGCTCTTCCGGTAGCTCTTCGAGCCCCATCACCTGTTTAGTGTTGGCAATGAGGAACGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCaa                                                                                                                                    >  1:639470/1‑150 (MQ=255)        cggcggTATTGATAAACGGCTCTTCCGGTAGCTCTTCGAGCCCCATCACCTGTTTAGTGTTGGCAATGAGGAACGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAAc                                                                                                                                   <  1:922195/151‑1 (MQ=255)        cggcggTATTGATAAACGGCTCTTCCGGTAGCTCTTCGAGCCCCATCACCTGTTTAGTGTTGGCAATGAGGAACGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAAc                                                                                                                                   <  1:947297/151‑1 (MQ=255)         ggcggTATTGATAAACGGCTCTTCCGGTAGCTCTTCGAGCCCCATCACCTGTTTAGTGTTGGCAATGAGGAACGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACg                                                                                                                                  >  1:530246/1‑151 (MQ=255)               aTTGATAAACGGCTCTTCCGGTAGCTCTTCGAGCCCCATCACCTGTTTAGTGTTGGCAATGAGGAACGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGAc                                                                                                                             <  2:216007/150‑1 (MQ=255)                 tGATAAACGGCTCTTCCGGTAGCTCTTCGAGCCCCATCACCTGTTTAGTGTTGGCAATGAGGAACGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACcg                                                                                                                           <  1:940267/150‑1 (MQ=255)                    tAAACGGCTCTTCCGGTAGCTCTTCGAGCCCCATCACCTGTTTAGTGTTGGCAATGAGGAACGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGcgtc                                                                                                                       >  1:165748/1‑151 (MQ=255)                      aaCGGCTCTTCCGGTAGCTCTTCGAGCCCCATCACCTGTTTAGTGTTGGCAATGAGGAACGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGcgtcgt                                                                                                                     >  1:264195/1‑151 (MQ=255)                      aaCGGCTCTTCCGGTAGCTCTTCGAGCCCCATCACCTGTTTAGTGTTGGCAATGAGGAACGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGcgtcgt                                                                                                                     <  2:34657/151‑1 (MQ=255)                                  ggcaGCTCTTCGAGCCCCATCACCTGTTTAGTGTTGGCAATGAGGAACGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCaaa                                                                                                          >  2:30285/4‑150 (MQ=255)                                  ggTAGCTCTTCGAGCCCCATCACCTGTTTAGTGTTGGCAATGAGGAACGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCaaa                                                                                                          >  1:868353/1‑150 (MQ=255)                                  ggTAGCTCTTCGAGCCCCATCACCTGTTTAGTGTTGGCAATGAGGAACGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCaaa                                                                                                          >  1:178305/1‑150 (MQ=255)                                    tAGCTCTTCGAGCCCCATCACCTGTTTAGTGTTGGCAATGAGGAACGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGc                                                                                                       >  1:386270/1‑151 (MQ=255)                                          ttCGAGCCCCATCACCTGTTTAGTGTTGGCAATGAGGAACGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATcc                                                                                                 >  2:808630/1‑151 (MQ=255)                                          ttCGAGCCCCATCACCTGTTTAGTGTTGGCAATGAGGAACGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATcc                                                                                                 >  2:332395/1‑151 (MQ=255)                                          ttCGAGCCCCATCACCTGTTTAGTGTTGGCAATGAGGAACGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATcc                                                                                                 >  1:173656/1‑151 (MQ=255)                                           tCGAGCCCCATCACCTGTTTAGTGTTGGCAATGAGGAACGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAAt                                                                                                   <  1:22427/148‑1 (MQ=255)                                             gAGCCCCATCACCTGTTTAGTGTTGGCAATGAGGAACGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCaaa                                                                                              <  2:676449/151‑1 (MQ=255)                                               gCCCCATCACCTGTTTAGTGTTGGCAATGAGGAACGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCaa                                                                                               >  1:883105/1‑148 (MQ=255)                                                 cccATCACCTGTTGAGTGTTGGCAATGAGGAACGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAgaga                                                                                          <  2:437396/151‑1 (MQ=255)                                                       aCCTGTTTAGTGTTGGCAATGAGGAACGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCtt                                                                                    >  1:183001/1‑151 (MQ=255)                                                          tGTTTAGTGTTAGCAATGAGGAACGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCACAGAGACATCTTTAc                                                                                 >  2:525116/1‑151 (MQ=255)                                                           gTTTAGTGTTGGCAATGAGGAACGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTAcc                                                                                >  1:201342/1‑151 (MQ=255)                                                               aGTGTTGGCAATGAGGAACGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGGCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCAc                                                                             <  1:1052793/150‑1 (MQ=255)                                                                  gtTGGCAATGAGGAACGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTc                                                                          >  2:619370/1‑150 (MQ=255)                                                                      gCAATGAGGAACGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAAt                                                                     <  1:990196/151‑1 (MQ=255)                                                                         aTGAGGAACGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTg                                                                   >  2:445283/1‑150 (MQ=255)                                                                           gAGGAACGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGt                                                                <  1:808676/151‑1 (MQ=255)                                                                               aaCGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCg                                                             >  2:817923/1‑150 (MQ=255)                                                                                 cGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTc                                                              >  1:559106/1‑147 (MQ=255)                                                                                 cGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAg                                                          <  2:132130/151‑1 (MQ=255)                                                                                          gTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATcccc                                                 <  2:173655/151‑1 (MQ=255)                                                                                           tCATAAACGCCCGGCGCATCTTCATTGGGGAAACCCGCTTTCATGGAACGGCACGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGCAGTGAATcc                                                   >  1:1069825/1‑148 (MQ=255)                                                                                             aTAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATc                                              <  2:674944/151‑1 (MQ=255)                                                                                               aaaCGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATcgc                                            >  2:1024378/1‑151 (MQ=255)                                                                                               aaaCGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATcg                                             <  2:917470/150‑1 (MQ=255)                                                                                               aaaCGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATcg                                             <  1:549251/150‑1 (MQ=255)                                                                                                      cGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAg                                     <  1:648695/151‑1 (MQ=255)                                                                                                        gcgcATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGa                                       >  1:727445/1‑147 (MQ=255)                                                                                                        gcgcATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGAt                                   <  1:894559/151‑1 (MQ=255)                                                                                                         cgcATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGAtt                                  <  1:822264/151‑1 (MQ=255)                                                                                                           cATCTTCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTc                                <  1:152836/151‑1 (MQ=255)                                                                                                                tCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCg                             <  1:116590/149‑1 (MQ=255)                                                                                                                tCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGAc                           >  2:8786/1‑151 (MQ=255)                                                                                                                tCATTGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGAc                           >  1:345055/1‑151 (MQ=255)                                                                                                                    tGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGc                        <  1:991819/150‑1 (MQ=255)                                                                                                                    tGGGTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGc                        <  1:543713/150‑1 (MQ=255)                                                                                                                      ggTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTg                     <  1:440638/151‑1 (MQ=255)                                                                                                                       gTAAACCCGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGc                    <  1:509478/151‑1 (MQ=255)                                                                                                                           aCCCGCTGTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTGGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCGGCCGGCGGGCcagc                <  2:816755/151‑1 (MQ=255)                                                                                                                            cccGCTTTCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCcagca               <  1:522108/151‑1 (MQ=255)                                                                                                                                 tttCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGAt          >  2:799492/1‑151 (MQ=255)                                                                                                                                  ttCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGa           <  1:764732/149‑1 (MQ=255)                                                                                                                                   tCATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGAttt        >  2:37029/1‑151 (MQ=255)                                                                                                                                    cATGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCCCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGAtttc       >  2:274537/1‑150 (MQ=255)                                                                                                                                     aTGGAACGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTAt      <  2:994847/151‑1 (MQ=255)                                                                                                                                         aaCGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCGCAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAg  <  2:845822/151‑1 (MQ=255)                                                                                                                                         aaCGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTAt      >  2:828861/1‑147 (MQ=255)                                                                                                                                         aaCGGCAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAg  <  1:69187/151‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                  TTAAGTCCGGCGGTATTGATAAACGGCTCTTCCGGTAGCTCTTCGAGCCCCATCACCTGTTTAGTGTTGGCAATGAGGAACGGCAGCGCGTCATAAACGCCCGGCGCATCTTCATTGGGTAAACCCGCTTTCATGGAACGGTAAGTGCCTACGCCAACGAAGACCGCGTCGTATTGTTCCAAAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAG  >  NC_000913/2584117‑2584403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑