Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,591,522	T→C	R92R (CGT→CGC)	yffB →	putative ArsC family reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,591,522	0	T	C	100.0%	131.2 / NA	38	R92R (CGT→CGC)	yffB	putative ArsC family reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/18);  total (20/18)

TTTATCAACGAATTAGGCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTG  >  NC_000913/2591379‑2591667                                                                                                                                                |                                                                                                                                                  tttATCAACGAATTAGGCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGCCCATTg                                                                                                                                             <  2:885299/150‑1 (MQ=255) tttATCAACGAATTAGGCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGCCCATTg                                                                                                                                             <  2:42259/150‑1 (MQ=255)   tATTAACGAATTAGGCTGGGAAGCGTTACTAAACACCTGAGGTTCAACCTGGCGTAACATGGACGAAACCACCCGCAATAAAATCAACGATGCGGCCTCTGCCGCGGCATTAATGACTGAAATGCCTGCAATTATCAACCGCCCATTGctc                                                                                                                                          >  2:932556/1‑151 (MQ=255)    ataaaCGAATTAGGCGGGGAAGCGTTACTCACCACCCGTGGTACAACCTGGCGTAAACCGGACGAAAACACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGCCCCTTGctct                                                                                                                                         >  2:704946/4‑151 (MQ=255)    aTCAACGAATTAGGCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGCCCATTGctct                                                                                                                                         >  1:1064916/1‑151 (MQ=255)                 gCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGCCCATTGCTCTGCGTGccc                                                                                                                                 >  1:636052/1‑146 (MQ=255)                 gCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGCCCATTGCTCTGCGTGCCCgg                                                                                                                               <  2:636018/148‑1 (MQ=255)                 gCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGCCCATTGCTCTGCGTGCCCGGTaa                                                                                                                            <  1:895745/151‑1 (MQ=255)                              aCTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGCCCATTGCTCTGCGTGCCCGGTAAGCCTATGctgctg                                                                                                               <  2:819825/151‑1 (MQ=255)                                 cAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGCCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGt                                                                                                            <  1:984951/151‑1 (MQ=255)                                  aaCACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGCCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGtt                                                                                                           <  2:988902/151‑1 (MQ=255)                                     acCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGCCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTc                                                                                                         >  1:1036866/1‑150 (MQ=255)                                     acCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGCCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTc                                                                                                         >  1:60677/1‑150 (MQ=255)                                     acCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGCCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCa                                                                                                        <  1:39933/151‑1 (MQ=255)                                      cccGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGCCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTc                                                                                                         >  2:115758/1‑149 (MQ=255)                                                 aCCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTAATGACCGAAATGCCTGCAATTATCAAACGCCCCTTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGGGATTCCAGtt                                                                                            >  2:572483/1‑151 (MQ=255)                                                               ggACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGCCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAAttttt                                                                               <  2:705099/150‑1 (MQ=255)                                                                gACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGCCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTcc                                                                             <  2:390023/151‑1 (MQ=255)                                                                    aaaCCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGCCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATg                                                                          >  2:327147/1‑150 (MQ=255)                                                                          cccGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGCCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGgtgt                                                                    <  2:668272/150‑1 (MQ=255)                                                                               aaTAAAATCACCGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGCCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCt                                                               >  2:615801/1‑150 (MQ=255)                                                                               aaTAAAATCACCGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGACTGCAATTATCAAACGCCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCt                                                               >  2:712926/1‑150 (MQ=255)                                                                                aTAAAATCACCGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGCCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTAt                                                             >  1:755846/1‑151 (MQ=255)                                                                                   aaaTCACCGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGCCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTc                                                          <  1:877500/151‑1 (MQ=255)                                                                                   aaaTCACCGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGCCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTc                                                          <  1:230100/151‑1 (MQ=255)                                                                                          cGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGCCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCg                                                   >  2:742741/1‑151 (MQ=255)                                                                                                 gCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGCCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTg                                            <  2:211354/151‑1 (MQ=255)                                                                                                 gCCGCTGAGGCGGCAATAATGACTGAAATGCCTGCAATTATCAAACGCCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTag                                              <  2:230481/149‑2 (MQ=255)                                                                                                        cggcggCATTAATGACTGAAATGCCTGCAATTATCAAACGCCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGac                                     >  1:595098/1‑151 (MQ=255)                                                                                                          gcggcATTAATGACTGAAATGCCTGCAATTATCAAACGCCCAGTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGaca                                    >  2:139338/1‑150 (MQ=255)                                                                                                                        tGAAATGCCTGCAATTATCAAACGCCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTcgc                     >  1:690771/1‑151 (MQ=255)                                                                                                                         gAAATGCCTGCAATTATCAAACGCCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTcg                      <  2:874890/149‑1 (MQ=255)                                                                                                                            aTGCCTGCAATTATCAAACGCCCATTGCTCTGCGTGCCCGGTACGCCTATGCTGCTTGGTTTCTGTGTTTCCTGTTATCAGCAATTTGTCCATGAGGTTTAGTCTATGTCCTGCCCGGTTATTGAGCCGACACAACAGCTTATTcgccgca                 <  2:853560/151‑2 (MQ=255)                                                                                                                              gCCTGCAATTATCAAACGCCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGccc                <  2:121697/150‑1 (MQ=255)                                                                                                                                    aaTTATCAAACGCCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTg         >  2:432287/1‑151 (MQ=255)                                                                                                                                     aTTATCAAACGCCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTg         >  1:793384/1‑150 (MQ=255)                                                                                                                                         tCAAACGCCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTcc    >  2:720689/1‑151 (MQ=255)                                                                                                                                            aaCGCCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCtg  >  2:525822/1‑150 (MQ=255)                                                                                                                                                |                                                                                                                                                  TTTATCAACGAATTAGGCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTG  >  NC_000913/2591379‑2591667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑