Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,754,034	G→A	R89* (CGA→TGA)	ratB ←	UPF0125 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,754,034	0	G	A	100.0%	328.0 / NA	83	R89* (CGA→TGA)	ratB	UPF0125 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/40);  total (43/40)

TTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAAATGCCGACTTTGTTTTTAGTTAAATCGATATCGGTACGCAATTCC  >  NC_000913/2753888‑2754179                                                                                                                                                   |                                                                                                                                                  ttAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTg                                                                                                                                                <  2:729229/150‑1 (MQ=255)   aaCAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCt                                                                                                                                             <  2:273390/151‑1 (MQ=255)       gTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGa                                                                                                                                         <  1:686782/151‑1 (MQ=255)          gCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGc                                                                                                                                      >  2:406621/1‑151 (MQ=255)           cGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGct                                                                                                                                     <  1:353576/151‑1 (MQ=255)           cGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGct                                                                                                                                     <  2:915518/151‑1 (MQ=255)            ggTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGctc                                                                                                                                    <  1:221115/151‑1 (MQ=255)                 gtTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTc                                                                                                                                <  2:988881/150‑1 (MQ=255)                 gtTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCg                                                                                                                               <  1:802455/151‑1 (MQ=255)                    gACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGAt                                                                                                                            >  1:922359/1‑151 (MQ=255)                    gACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGAt                                                                                                                            <  2:651480/151‑1 (MQ=255)                      ccAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCg                                                                                                                          >  2:65464/1‑151 (MQ=255)                       cAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCgg                                                                                                                         <  1:648050/151‑1 (MQ=255)                        aaTATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGc                                                                                                                        >  1:763992/1‑151 (MQ=255)                         atatTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGc                                                                                                                        >  1:464689/1‑150 (MQ=255)                            ttGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGc                                                                                                                                           >  2:552335/1‑128 (MQ=255)                            ttGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGc                                                                                                                                           <  1:552366/128‑1 (MQ=255)                                 aaCAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGagag                                                                                                                <  1:779593/150‑1 (MQ=255)                                   cAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGc                                                                                                             <  1:1010031/151‑1 (MQ=255)                                   cAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGc                                                                                                             >  1:804748/1‑151 (MQ=255)                                    aaaCCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCg                                                                                                            >  2:580787/1‑151 (MQ=255)                                       ccTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATa                                                                                                         <  2:765574/151‑1 (MQ=255)                                          gcgcTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAAt                                                                                                      <  1:768180/151‑1 (MQ=255)                                              tGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTcc                                                                                                  >  1:759107/1‑151 (MQ=255)                                                aGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCAc                                                                                                <  2:817791/151‑1 (MQ=255)                                                aGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCAc                                                                                                <  1:333439/151‑1 (MQ=255)                                                   ggTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCAccc                                                                                              <  2:646660/150‑1 (MQ=255)                                                      aaCTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGAt                                                                                           <  2:595554/150‑1 (MQ=255)                                                      aaCTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATc                                                                                          <  2:747491/151‑1 (MQ=255)                                                       aCTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCg                                                                                         >  1:224706/1‑151 (MQ=255)                                                         taataataaAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGcc                                                                                       >  2:743993/1‑151 (MQ=255)                                                          aataataaAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACATTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCca                                                                                      <  2:571835/151‑1 (MQ=255)                                                           ataataaAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCcat                                                                                     <  1:332240/151‑1 (MQ=255)                                                            taataaAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCcatc                                                                                    >  1:825866/1‑151 (MQ=255)                                                              ataaAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCcatcat                                                                                  >  2:849132/1‑151 (MQ=255)                                                                 aaGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGca                                                                               <  1:184630/151‑1 (MQ=255)                                                                     tGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACt                                                                           >  1:534292/1‑151 (MQ=255)                                                                       ctctCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTAt                                                                         >  1:983402/1‑151 (MQ=255)                                                                            aaaGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCtt                                                                    <  2:723505/151‑1 (MQ=255)                                                                               gACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAg                                                                  >  2:892566/1‑150 (MQ=255)                                                                                 cGTTAAAAAAGGTGCTCAATGAGCACCTTTCTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGttt                                                               >  1:867668/1‑151 (MQ=255)                                                                                    tAAAAAAGGTGCTCAATGAGCGCCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGTGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTg                                                             <  2:694727/150‑1 (MQ=255)                                                                                    tAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTg                                                             <  1:849180/150‑1 (MQ=255)                                                                                     aaaaaaGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGcc                                                           >  1:677258/1‑151 (MQ=255)                                                                                     aaaaaaGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGc                                                            <  2:1028219/150‑1 (MQ=255)                                                                                         aaGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGAc                                                       >  1:911246/1‑151 (MQ=255)                                                                                            gTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGc                                                    >  2:310651/1‑151 (MQ=255)                                                                                            gTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATCGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGc                                                    >  2:762176/1‑151 (MQ=255)                                                                                                 cAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTaa                                               <  1:764295/151‑1 (MQ=255)                                                                                                  aaTGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTaa                                               >  2:334285/1‑150 (MQ=255)                                                                                                  aaTGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTaa                                               >  2:960183/1‑150 (MQ=255)                                                                                                    tGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATAAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTa                                                >  2:358168/1‑147 (MQ=255)                                                                                                     gAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAAAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAAATg                                            <  1:687797/150‑1 (MQ=255)                                                                                                       gCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAAATGCCg                                         <  2:75764/151‑1 (MQ=255)                                                                                                           cTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAAATGCCGACtt                                     >  2:262279/1‑151 (MQ=255)                                                                                                             tttttCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAAATGCCGACttt                                    <  2:450974/150‑1 (MQ=255)                                                                                                              ttttCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAAATGCCGCCTTTGt                                  >  2:580439/1‑151 (MQ=255)                                                                                                              ttttCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAAATGCCGACTTTGt                                  >  1:740577/1‑151 (MQ=255)                                                                                                               tttCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAAATGCCGACTTTGt                                  <  2:94557/150‑1 (MQ=255)                                                                                                                 tctgtctgTTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAAATGCCGACTTTGttt                                <  1:895036/150‑1 (MQ=255)                                                                                                                  ctgtctgtTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAAATGCCGACTTTGttttt                              >  2:683589/1‑151 (MQ=255)                                                                                                                    gtctgtTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAAATGCCGACTTTGttttt                              <  1:513332/149‑1 (MQ=255)                                                                                                                      ctgtTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAAATGCCGACTTTGTTTTTAGtt                          >  2:601354/1‑151 (MQ=255)                                                                                                                      ctgtTATTTATTCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAAATGCCGACTTTGTTTTTAGtt                          >  1:902271/1‑151 (MQ=255)                                                                                                                          tatttattCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAAATGCCGACTTTGTTTTTAGTTAAAt                      >  2:410854/1‑151 (MQ=255)                                                                                                                           atttattCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAAATGCCGACTTTGTTTTTAGTTAAATc                     >  2:197612/1‑151 (MQ=255)                                                                                                                             ttattCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGTCGGCTGTAAATGCCGACTTTGTTTTTAGTTAAATCg                    <  1:964899/150‑1 (MQ=255)                                                                                                                             ttattCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAAATGCCGACTTTGTTTTTAGTTAAATCg                    <  1:981363/150‑1 (MQ=255)                                                                                                                             ttattCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAAATGCCGACTTTGTTTTTAGTTAAATCg                    <  2:545905/150‑1 (MQ=255)                                                                                                                             ttattCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAAATGCCGACTTTGTTTTTAGTTAAATCg                    >  1:940409/1‑150 (MQ=255)                                                                                                                                ttCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAAATGCCGACTTTGTTTTTAGTTAAATCGatat                >  2:166517/1‑151 (MQ=255)                                                                                                                                 tCGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAAATGCCGACTTTGTTTTTAGTTAAATCGATATc               >  1:1057254/1‑151 (MQ=255)                                                                                                                                  cGCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAAATGCCGACTTTGTTTTTAGTTAAATCGATATCg              >  1:1016733/1‑151 (MQ=255)                                                                                                                                   gCTGATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAAATGCCGACTTTGTTTTTAGTTAAATCGATATCgg             <  2:493756/151‑1 (MQ=255)                                                                                                                                      gATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAAATGCCGACTTTGTTTTTAGTTAAATCGATATCGGt            <  1:506928/149‑1 (MQ=255)                                                                                                                                      gATTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAAATGCCGACTTTGTTTTTAGTTAAATCGATATCGGTAc          >  1:585877/1‑151 (MQ=255)                                                                                                                                       aTTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAAATGCCGACTTTGTTTTTAGTTAAATCGATATCGGTa           <  1:686063/149‑1 (MQ=255)                                                                                                                                       aTTTTTCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAAATGCCGACTTTGTTTTTAGTTAAATCGATATCGGTACg         >  1:1020025/1‑151 (MQ=255)                                                                                                                                        tttttCTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAAATGCCGACTTTGTTTTTAGTTAAATCGATATCGGTACGc        >  1:296038/1‑151 (MQ=255)                                                                                                                                             cTGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAAATGCCGACTTTGTTTTTAGTTAAATCGATATCGGTACGCAATTc   >  1:1027886/1‑151 (MQ=255)                                                                                                                                              tGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAAATGCCGACTTTGTTTTTAGTTAAATCGATATCGGTACGCAATTcc  >  2:1030501/1‑151 (MQ=255)                                                                                                                                              tGCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAAATGCCGACTTTGTTTTTAGTTAAATCGATATCGGTACGCAATTcc  >  1:95394/1‑151 (MQ=255)                                                                                                                                               gCTCATTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAAATGCCGACTTTGTTTTTAGTTAAATCGATATCGGTACGCAATTcc  >  2:64640/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                  TTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAAATGCCGACTTTGTTTTTAGTTAAATCGATATCGGTACGCAATTCC  >  NC_000913/2753888‑2754179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑