Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,780,291 G→A G812G (GGC→GGT ypjA ← adhesin‑like autotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,780,2910GA98.6% 257.2 / NA 72G812G (GGC→GGTypjAadhesin‑like autotransporter
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (36/35);  total (36/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTG  >  NC_000913/2780143‑2780432
                                                                                                                                                     |                                                                                                                                             
cATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTAcc                                                                                                                                             >  1:165701/1‑151 (MQ=255)
  ttATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCt                                                                                                                                            <  1:633858/150‑1 (MQ=255)
  ttATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTg                                                                                                                                           >  1:179398/1‑151 (MQ=255)
       gTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGc                                                                                                                                       >  2:65733/1‑150 (MQ=255)
          gACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTg                                                                                                                                   >  2:962216/1‑151 (MQ=255)
            cTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGc                                                                                                                                 >  2:632632/1‑151 (MQ=255)
             tGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCt                                                                                                                                >  1:442596/1‑151 (MQ=255)
                  gCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCttttgt                                                                                                                           >  1:761377/1‑151 (MQ=255)
                  gCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCCCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCCCCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCttt                                                                                                                              <  2:443889/148‑1 (MQ=255)
                       cctctttCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCTTTAATTTTTGTCTCATTCGCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCttttgttttg                                                                                                                       <  2:138224/145‑1 (MQ=255)
                          ccTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACg                                                                                                                    >  2:943909/1‑150 (MQ=255)
                           cTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGAt                                                                                                                  >  1:367257/1‑151 (MQ=255)
                             tCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGAtct                                                                                                                >  1:393533/1‑151 (MQ=255)
                               aTTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGAtctc                                                                                                               <  1:673072/150‑1 (MQ=255)
                                ttGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTcc                                                                                                              <  1:454165/150‑1 (MQ=255)
                                ttGATCTTTGTTTTTATTCCCTTTCCGTTAGCTGAAACTGTTTGTATTCCGCCTTCGTTATTTTTTTTCTCTTCCGCCACGCCCTCTACAATTTGTTCCCCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGAtctc                                                                                                               <  1:799872/149‑1 (MQ=255)
                                 tGATCTTTGTTGTGATTGCTCTGCGGTTGGCTCAAACTTTGTATATGGTGCCGACGTTACTTGTTGCCTCTATCGCTCCGCCCGCGAGTATTTGTACACCGCCGGGGAGGGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGAt                                                                                                                  <  2:933621/145‑1 (MQ=255)
                                 tGATATTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAAATGTTGTCTCATTAACCACACCCTCGACAATTTTCTCAGCGCCGGTGAGCGTCATACCTGTCGCAGTGGCTGAAGATTTGACGGTCTTCCg                                                                                                            >  2:214516/1‑151 (MQ=255)
                                   aTCTTTTTTTTTATTCCCTCCCCGTTAGCTGAAACTGTTTGTATGCCGCCGTCTTTAATTTTTGTCTCATTCGCCTCTCCCTCTACAATTTGTTCACCGCCGGTGAGCGTCTTACCTGTCGCAGTGGCTTTTTTTTTGACTATCTCCCGt                                                                                                           <  2:525247/150‑1 (MQ=255)
                                   aTCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTc                                                                                                          >  1:207759/1‑151 (MQ=255)
                                        tGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGccc                                                                                                     >  2:625303/1‑151 (MQ=255)
                                           ttttATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCat                                                                                                   >  2:407977/1‑150 (MQ=255)
                                            tttATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCata                                                                                                  <  2:781762/150‑1 (MQ=255)
                                            tttATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCatat                                                                                                 >  2:470456/1‑151 (MQ=255)
                                            tttATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCatat                                                                                                 >  1:727505/1‑151 (MQ=255)
                                            tttATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCata                                                                                                  <  2:152312/150‑1 (MQ=255)
                                             ttATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCatat                                                                                                 <  2:532791/150‑1 (MQ=255)
                                              tATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTg                                                                                               >  1:654475/1‑151 (MQ=255)
                                               aTTGCCTCTCCGTTAGCTGAACCTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACCCCCCCGACAATTGGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATAtt                                                                                                <  1:104408/149‑1 (MQ=255)
                                                ttGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGAc                                                                                             >  1:571225/1‑151 (MQ=255)
                                                    ctctCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCtgt                                                                                         >  1:511068/1‑151 (MQ=255)
                                                     tctcCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCtgtg                                                                                        >  2:881096/1‑151 (MQ=255)
                                                      ctcCGTTAGCTGAAATTGATTGTATTTTGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCCCCAGTGAGCGTCGTACCTGTCGCAGCGGCTTTTGTTTCTACGATCTCCAGTCCGCCCATATTGACCTgtgt                                                                                       >  2:781438/1‑151 (MQ=255)
                                                          cGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGtttt                                                                                    >  1:271384/1‑151 (MQ=255)
                                                           gTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTATTTTTTGTCTCATTCGCCCCCCCCTCGACATTTTTTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGtttt                                                                                    <  2:1025547/150‑1 (MQ=255)
                                                           gTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTg                                                                                   >  1:460961/1‑151 (MQ=255)
                                                            ttAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGt                                                                                  >  2:324810/1‑151 (MQ=255)
                                                             tAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTc                                                                                 >  2:428323/1‑151 (MQ=255)
                                                              aGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTc                                                                                 >  1:254197/1‑150 (MQ=255)
                                                                  gAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCaga                                                                              <  1:623947/149‑1 (MQ=255)
                                                                  gAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCaga                                                                              >  2:468846/1‑149 (MQ=255)
                                                                    aaCTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAagag                                                                          <  1:605061/151‑1 (MQ=255)
                                                                        gTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGgtg                                                                       <  1:943958/150‑1 (MQ=255)
                                                                           tGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCt                                                                    <  1:308660/150‑1 (MQ=255)
                                                                           tGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTg                                                                   >  1:334591/1‑151 (MQ=255)
                                                                            gTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTg                                                                   <  2:467717/150‑1 (MQ=255)
                                                                              aTTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACt                                                                >  2:944742/1‑151 (MQ=255)
                                                                                      gtcgtTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGtt                                                        >  1:604944/1‑151 (MQ=255)
                                                                                        cgtTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTaa                                                      <  2:615389/151‑1 (MQ=255)
                                                                                         gtTAATTGTTGTCTCATTCGCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTaa                                                      <  1:352067/150‑1 (MQ=255)
                                                                                              ttgttgTCTCTTTCGCCCCCCCCTCTACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACAcgc                                                 <  2:19248/150‑1 (MQ=255)
                                                                                               tgttgtCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACAcgccg                                               >  2:46804/1‑151 (MQ=255)
                                                                                                  tgtCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACgccgcc                                             >  2:717194/1‑150 (MQ=255)
                                                                                                       cATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGGGTCTGACTCCACGGTTAACACCCCCCCATTTGc                                       >  1:1055109/1‑151 (MQ=255)
                                                                                                        aTTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGcc                                      >  2:2542/1‑151 (MQ=255)
                                                                                                               acccccTCGACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCTGGa                               <  1:470481/148‑1 (MQ=255)
                                                                                                                   cccctccattttGTGCGCCGCCGGTGAGCGTCGTACCAGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGGCAGAAGAGGTGTCTGACTCCTCGGTTAACACGCCGCCATTTGCCAGCAGGa                               <  2:697590/138‑1 (MQ=255)
                                                                                                                       gACAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGtt                        <  1:730112/150‑1 (MQ=255)
                                                                                                                       gACAATTTGTTCAACGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTc                       >  2:945616/1‑151 (MQ=255)
                                                                                                                         cAATTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTcg                      <  2:571194/150‑1 (MQ=255)
                                                                                                                           aTTTGTTCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTcgccg                   <  1:254231/151‑1 (MQ=255)
                                                                                                                            tttGTTCTCCGCCGGTGAGCGTCGTACCTGTCGCAGTTGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTcgccg                   <  2:59695/150‑1 (MQ=255)
                                                                                                                                 tCACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCAcc               <  2:257856/149‑1 (MQ=255)
                                                                                                                                  cACCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCAAGCAGGATATTGTTCGCCGCACCCTg            >  2:603605/1‑151 (MQ=255)
                                                                                                                                   aCCGCCGGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCAccc              <  2:5905/148‑1 (MQ=255)
                                                                                                                                     cgccgGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTc         <  1:97039/151‑1 (MQ=255)
                                                                                                                                     cgccgGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTc         <  2:65169/151‑1 (MQ=255)
                                                                                                                                     cgccgGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTc         <  1:470700/151‑1 (MQ=255)
                                                                                                                                      gccgGTGAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCg        <  2:777009/151‑1 (MQ=255)
                                                                                                                                            gAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCt   <  1:332532/150‑1 (MQ=255)
                                                                                                                                            gAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTg  <  2:745421/151‑1 (MQ=255)
                                                                                                                                            gAGCGTCGTACCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTg  <  1:226201/151‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                             
CATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTG  >  NC_000913/2780143‑2780432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: