Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,811,185	T→C	L139P (CTC→CCC)	mprA →	transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,811,185	0	T	C	100.0%	278.6 / NA	73	L139P (CTC→CCC)	mprA	transcriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/32);  total (41/32)

GCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGATTTATA  >  NC_000913/2811043‑2811332                                                                                                                                               |                                                                                                                                                    gCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTcc                                                                                                                                              <  1:296881/150‑1 (MQ=255)   cGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCcgcg                                                                                                                                           >  1:119289/1‑151 (MQ=255)    gATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCgcgc                                                                                                                                          >  1:878493/1‑151 (MQ=255)         aCTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGc                                                                                                                                     <  2:442537/151‑1 (MQ=255)         aCTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGc                                                                                                                                     >  1:423836/1‑151 (MQ=255)           tGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGcac                                                                                                                                   >  1:266525/1‑151 (MQ=255)                aaaCGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAg                                                                                                                              <  1:906203/151‑1 (MQ=255)                   cgcgGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGaaa                                                                                                                           <  2:117163/151‑1 (MQ=255)                     cgGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGaaaaa                                                                                                                         <  1:559821/151‑1 (MQ=255)                      ggTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAg                                                                                                                        <  2:318627/151‑1 (MQ=255)                        ttGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGaaaa                                                                                                                          >  1:341523/1‑147 (MQ=255)                        ttGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGaaaa                                                                                                                          >  2:875288/1‑147 (MQ=255)                                 aCGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCg                                                                                                               >  2:504977/1‑149 (MQ=255)                                  cgtcgtGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGc                                                                                                            <  1:147471/151‑1 (MQ=255)                                     cgtGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCaaa                                                                                                         <  2:373809/151‑1 (MQ=255)                                     cgtGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCaa                                                                                                          <  1:402417/150‑1 (MQ=255)                                      gtGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAAt                                                                                                        <  1:991117/151‑1 (MQ=255)                                            gCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCg                                                                                                  <  1:161996/151‑1 (MQ=255)                                                tAACGATCGCCGCTTCCTGCATCCGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGc                                                                                                 >  2:122678/1‑148 (MQ=255)                                                      tCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGctc                                                                                        >  2:456157/1‑151 (MQ=255)                                                        gccgcTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGTTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGctct                                                                                       <  2:832885/150‑1 (MQ=255)                                                        gccgcTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCtctc                                                                                      >  2:252605/1‑151 (MQ=255)                                                             tgcctgcATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGtc                                                                                 >  1:64681/1‑151 (MQ=255)                                                              gcctgcATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGtct                                                                                >  1:40460/1‑151 (MQ=255)                                                              gcctgcATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGtc                                                                                 <  1:135884/150‑1 (MQ=255)                                                                         catttAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATgg                                                                     >  2:635305/4‑151 (MQ=255)                                                                         cAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATg                                                                      <  2:594553/150‑1 (MQ=255)                                                                             tAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAAc                                                                  >  2:197321/1‑150 (MQ=255)                                                                               aCGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACa                                                                 >  2:796789/1‑149 (MQ=255)                                                                               aCGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACa                                                                 >  2:320383/1‑149 (MQ=255)                                                                                cGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACa                                                                 >  2:513776/1‑148 (MQ=255)                                                                                cGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGa                                                              <  1:46529/151‑1 (MQ=255)                                                                                   aaaaaGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACgg                                                           >  1:929533/1‑151 (MQ=255)                                                                                   aaaaaGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACgg                                                           >  1:649308/1‑151 (MQ=255)                                                                                        ggTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTgg                                                      >  1:775280/1‑151 (MQ=255)                                                                                          tCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGtt                                                    >  1:384671/1‑151 (MQ=255)                                                                                           cACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTtc                                                   >  2:289034/1‑151 (MQ=255)                                                                                           cACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTtc                                                   <  1:870020/151‑1 (MQ=255)                                                                                           cACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTtc                                                   >  1:878011/1‑151 (MQ=255)                                                                                           cACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTtc                                                   <  1:1032320/151‑1 (MQ=255)                                                                                            aCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTTCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAACTTGCTCTCCCGTCTCGACCAGACAGAACAAGACGGCGTGGTtct                                                  >  2:427324/1‑151 (MQ=255)                                                                                              gAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTtctc                                                 >  2:970934/1‑150 (MQ=255)                                                                                                gTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGa                                               >  1:1008782/1‑150 (MQ=255)                                                                                                  ttttGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGc                                            >  1:521383/1‑151 (MQ=255)                                                                                                   tttGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGc                                            <  1:378822/150‑1 (MQ=255)                                                                                                   tttGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGCAACAAGACGGTGTGGTTCTCGAAGCg                                           >  2:512233/1‑151 (MQ=255)                                                                                                     tGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGa                                          <  1:475960/150‑1 (MQ=255)                                                                                                       cgcgAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGa                                       <  2:737323/151‑1 (MQ=255)                                                                                                          gAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCt                                    >  1:161358/1‑151 (MQ=255)                                                                                                          gAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCt                                    >  1:710076/1‑151 (MQ=255)                                                                                                           aGGTTTTACCACCGCAGCATAACTGCCTGCATCAACCCTGGCCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTa                                   <  2:286233/151‑1 (MQ=255)                                                                                                                ttACCACCGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAAcgc                               >  2:644869/1‑150 (MQ=255)                                                                                                                  accaccGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTc                            >  1:785889/1‑151 (MQ=255)                                                                                                                   ccaccGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGCCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCa                           <  1:1050357/151‑1 (MQ=255)                                                                                                                    caccGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCAt                          >  2:1061170/1‑151 (MQ=255)                                                                                                                     accGCAGCATAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCAtc                         >  1:674365/1‑151 (MQ=255)                                                                                                                           gcaTAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTc                   <  1:910800/151‑1 (MQ=255)                                                                                                                           gcaTAACTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTc                   <  1:232885/151‑1 (MQ=255)                                                                                                                               aaCTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCaaa                >  1:590140/1‑150 (MQ=255)                                                                                                                               aaCTGCCTGCATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCaaa                <  1:439003/150‑1 (MQ=255)                                                                                                                                  tgcctgcATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATc            >  2:682480/1‑151 (MQ=255)                                                                                                                                   gcctgcATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAAt             <  1:778106/149‑1 (MQ=255)                                                                                                                                   gcctgcATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATcc           >  1:306197/1‑151 (MQ=255)                                                                                                                                   gcctgcATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATcc           <  1:440839/151‑1 (MQ=255)                                                                                                                                   gcctgcATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATcc           <  2:936351/151‑1 (MQ=255)                                                                                                                                   gcctgcATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATc            <  1:763996/150‑1 (MQ=255)                                                                                                                                       gcATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAg         <  1:122494/149‑1 (MQ=255)                                                                                                                                       gcATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGAt       <  1:759672/151‑1 (MQ=255)                                                                                                                                        cATCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGAtt      >  2:1029005/1‑151 (MQ=255)                                                                                                                                          tCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGAttt     >  1:212630/1‑150 (MQ=255)                                                                                                                                          tCAACCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGAttt     >  2:1004750/1‑150 (MQ=255)                                                                                                                                            aaCCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGATTtata  <  1:610638/151‑1 (MQ=255)                                                                                                                                            aaCCCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGATTtat   >  2:833660/1‑150 (MQ=255)                                                                                                                                               |                                                                                                                                                    GCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGATTTATA  >  NC_000913/2811043‑2811332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑