Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,628,616	IS2 (+) +5 bp	coding (319‑323/2244 nt)	yfgF ←	cyclic‑di‑GMP phosphodiesterase, anaerobic

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2070271	NA (NA)	50 (0.810)	48/298	0.5	100%	noncoding (1/1331 nt)	IS2	repeat region
	?	NC_000913	= 2628620	0 (0.000)					coding (319/2244 nt)	yfgF	cyclic‑di‑GMP phosphodiesterase, anaerobic
*	?	NC_000913	2628616 =	0 (0.000)	47 (0.760)	40/298	1.0	100%	coding (323/2244 nt)	yfgF	cyclic‑di‑GMP phosphodiesterase, anaerobic
	?	NC_000913	2996361 =	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region

AGCTGGGCGGCATTGCCAGGGATCGCCTTAGGGATTTTTGTCCGCAAATATGCAGAGCTGGGTTTTTACGAAACGCTATCATTAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTACAGGGTCTTTACTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2628758‑2628616 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCT  >  NC_000913/2996361‑2996510                                                                                                                                                                                                                                                                                                       AGCTGGGCGGCATTGCCAGGGATCGCCTTAGGGATTTTTGTCCGCAAATATGCAGAGCTGGGTTTTTACGAAACGCTATCATTAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTACAGGGGCTTTTATCCTGGATTT                                                                                                                                                 >  2:953776/1‑150     GGGCGGCATTGCCAGGGATCGCCTTAGGGATTTTTGTCCGCAAATATGCAGAGCTGGGTTTTTACGAAACGCTATCATTAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTTAAAGGGCTTTTATCCTGGGTTTTCCCC                                                                                                                                            >  1:776647/1‑151      GGCGGCATTGCCAGGGATCGCCTTAGGGATTTTTGTCCGCAAATATGCAGAGCTGGGTTTTTACGAAACGCTATCATTAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTTCAAGGGCTTTTCTCCTGGATTTTCCCC                                                                                                                                            >  1:1009878/1‑150      GGCGGCATTGCCAGGGATCGCCTTAGGGATTTTTGTCCGCAAATATGCAGAGCTGGGTTTTTACGAAACGCTATCATTAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTACAGGGTCTTTACTCCTGGATTTGCC                                                                                                                                              <  2:43621/148‑1        CGGCATTGCCAGGGATCGCCTTAGGGATTTTTGTCCGCAAATATGCAGAGCTGGGTTTTTACGAAACGCTATCATTAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTACAGGGTCTTTACTCCTGGATTTGCCCCTAT                                                                                                                                         <  2:944306/151‑1          GCATTGCCAGGGATCGCCTTAGGGATTTTTGTCCGCAAATATGCAGAGCTGGGTTTTTACGAAACGCTATCATTAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTACAGGGTCTTTACTCCTGGATTTGCCCCTATAT                                                                                                                                       <  1:457821/151‑1              TGCCAGGGATCGCCTTAGGGATTTTTGTCCGCAAATATGCAGAGCTGGGTTTTTACGAAACGCTATCATTAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTACAGGGTCTTTACTCCTGGATTTGCCCCTATATTTC                                                                                                                                    <  2:92587/150‑1                        CGCCTTAGGGATTTTTGTCCGCAAATATGCAGAGCTGGGTTTTTACGAAACGCTATCATTAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTACAGGGTCTTTACTCCTGGATTTGCCCCTATATTTCCAGACATCTG                                                                                                                          <  1:85845/150‑1                          CCTTAGGGATTTTTGTCCGCAAATATGCAGAGCTGGGTTTTTACGAAACGCTATCATTAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTTCAGGGGCTTTTCTCCTGGGTTTTCCCCTCTATTTTCAGACATCTGTT                                                                                                                        >  1:651640/1‑150                          CCTTAGGGATTTTTGTCCGCAAATATGCAGAGCTGGGTTTTTACGAAACGCTATCATTAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTACAGGGTCTTTACTTCTTGATTTGCCCCCATATTTTCATACATCTGTT                                                                                                                        >  1:917162/1‑150                          CCTTAGGGATTTTTGTCCGCAAATATGCAGAGCTGGGTTTTTACGAAACGCTATCATTAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTACAGGGTCTTTACTCCTGGATTTGCCCCTATATTTCCAGACATCTGTTA                                                                                                                       <  1:249964/151‑1                              AGGGATTTTTGTCCGCAAATATGCAGAGCTGGGTTTTTACGAAACGCTATCATTAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTTCAGGGTCTTTACTCCTTGATTTTTCCCTATATTTTCAGACATCTGTTAT                                                                                                                      >  1:318800/1‑148                                GGATTTTTGTCCGCAAATATGCAGAGCTGGGTTTTTACGAAACGCTATCATTAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTTCAAGGGCTTTACTCCTGGATTTTCTCCTATATTTCCCGACATCTGTTATTACTT                                                                                                                 >  1:844636/1‑151                                    TTTTGTCCGCAAATATGCAGAGCTGGGTTTTTACGAAACGCTATCATTAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTTCAGGGGGTTTTCTCCTTGATTTTCCCCTATATTTCCAGAAATCTGTTATTACTTTACC                                                                                                             >  1:883603/1‑151                                    TTTTGTCCGCAAATATGCAGAGCTGGGTTTTTACGAAACGCTATCATTAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTACAGGGTCTTTATTCCTGGATTTTCCCCCATATTTTCAGACATCTGTTATCACTTAACC                                                                                                             >  1:97143/1‑151                                       TGTCCGCAAATATGCAGAGCTGGGTTTTTACGAAACGCTATCATTAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTACAGGGGCTTTACTCCTGGATTTGCCCCTATATTTTCAGACATCTGTTTTCACTTAAACC                                                                                                            >  2:976644/1‑149                                                ATATGCAGAGCTGGGTTTTTACGAAACGCTATCATTAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTACAGGGGCTTTACTCCTGGATTTTCCCCTATATTTCCCGACATCTGTTATCACTTTACCCATTACAAACCC                                                                                                 >  2:421725/1‑151                                                       GAGCTGGGTTTTTACGAAACGCTATCATTAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTACAGGGTCTTTACTCCTGGATTTGCCCCTATATTGCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCG                                                                                          <  1:90810/151‑1                                                        AGCTGGGTTTTTACGAAACGCTATCATTAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTAAAGGGGCTTTAATCCTGGATTTGCCCCCATATTTCCAGACATCTGTTATCACTTTACCCATTACAAACCCGCTGgc                                                                                           >  2:593689/1‑147                                                                 TTTACGAAACGCTATCATTAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTACAAGGTCTTTACTCCTGGATTTTCCCCTATATTTCCAGACATCTGTTATTACCTAACCCATTACAAACCCCCTGCCGCAGATATTtc                                                                                >  2:987602/1‑149                                                                   TACGAAACGCTATCATTAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTACAGGGGCTTTACTCCTGGGTTTTCCCCTATATTTTCCGACATCTGTTTTCACTTAAACCATTACAAACCCGCTGCCCCAGATATTTCC                                                                               >  2:443525/1‑150                                                                     CGAAACGCTATCATTAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTAAAGGGGCTTTACTCCTGGATTTTCCCCTATATTTTCAGACATCTGTTATCACTTAACCCATTTCAAGCCCCCTGCCGCAGATATTCCC                                                                               >  1:708351/1‑148                                                                        AACGCTATCATTAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTACAAGGTCTTTAATCCTGGATTTGCCCCTATATTTCCAGACCACTTTTATCCCTTAACCCATTACAAGCCCGCCGCCCCAGATATTCCCGgg                                                                            >  2:838069/1‑146                                                                         ACGCTATCATTAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTACAGGGTCTTTACTCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGA                                                                        <  2:883636/151‑1                                                                         ACGCTATCATTAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTACAGGGTCTTTACTCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGA                                                                        <  2:97145/151‑1                                                                           GCTATCATTAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTACAGGGTCTTTACTCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGC                                                                      <  2:681414/151‑1                                                                           GCTATCATTAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTACAGGGTCTTTACTCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGC                                                                      <  2:579213/151‑1                                                                             TATCATTAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTACAGGGGCTTTACTCCTGGGTTTTCCCCTATATTTCCCGACATCTGTTATCACTTTACCCCTTACAAGCCCGCTGCCGCAGATATTCCCCTGGCGAGCG                                                                     >  2:49941/1‑150                                                                              ATCATTAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTTCAGGGTCTTTACTCCTGGATTTTTCCCTATATTTCCAGACATCTTTTATCACTTAACCCATTACAAACCCCCTGCCGCAGATATTCCCCTGGCGAGCGA                                                                    >  1:285571/1‑150                                                                                CATTAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTACAGGGTCTTTACTCCTGGATTTTCCCCTATATTTTCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCCTGGCGAGCGATA                                                                  >  2:1006764/1‑150                                                                                 ATTAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTACAGGGTCTTTACTCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAAC                                                                <  2:42133/151‑1                                                                                   TAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTACAGGGGCTTTTCTCCTGGATTTGCCCCCATATTTTCAGACATCTGTTTTTACTTAAACCATTACAAACCCGCCGCCGCAGATATTCCCGCGGCGAGCGATAA                                                                 >  2:232748/1‑148                                                                                     ACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTACCGGGGCTTTTCTCCTGGGTTTGCCCCTATATTTCCAGACATCCGGTATCACTTTACCCATTACAAGCCCCCTGCCGCAGATATTCCCGTGGCGGGCGATAAACC                                                              >  2:369541/1‑149                                                                                      CGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTACAGGGTCTTTACTCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGC                                                           <  1:5464/151‑1                                                                                           AATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTACAGGGTCTTTACTCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACT                                                      <  2:76797/151‑1                                                                                              TTTATTATCATTATCATTCTCTGTTGGGGCGGTTACAGGGTCTTTACTCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTAT                                                    <  1:463017/150‑1                                                                                               TTATTATCATTATCATTCTCTGTTGGGGCGGTTACAGGGTCTTTACTCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATG                                                   <  1:168630/150‑1                                                                                                   TATCATTATCATTCTCTGTTGGGGCGGTTACAGGGTCTTTACTCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGA                                               <  2:802047/150‑1                                                                                                    ATCATTATCATTCTCTGTTGGGGCGGTTACAGGGTCTTTACTCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGAT                                              <  1:242352/150‑1                                                                                                        TTATCATTCTCTGTTGGGGCGGTTACAGGGTCTTTACTCCTTGATTTTCCCCTATATTTCCAGACATCTTTTATCACTTAACCCATTACAAACCCCCTGCCGCAGAAATTCCCGTGGCGAGCGATAACCCCACGCACCATGCGGATGCCA                                          >  1:833243/1‑150                                                                                                             ATTCTCTGTTGGGGCGGTTACAGGGTCTTTACTCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTT                                    <  1:954070/151‑1                                                                                                                    GTTGGGGCGGTTACAGGGTCTTTACTCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAA                                <  2:63602/148‑1                                                                                                                                  AGGGTCTTTACTCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGC               >  2:640732/1‑151                                                                                                                                         TTACTCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGG           <  2:396380/148‑1                                                                                                                                              CCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCCCTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGC   <  2:705684/151‑1                                                                                                                                              CCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTG    <  2:441560/150‑1                                                                                                                                               CTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCT  >  1:787565/1‑151                                                                                                                                                                                                                                                                                                       AGCTGGGCGGCATTGCCAGGGATCGCCTTAGGGATTTTTGTCCGCAAATATGCAGAGCTGGGTTTTTACGAAACGCTATCATTAACGGCTAATTTTATTATCATTATCATTCTCTGTTGGGGCGGTTACAGGGTCTTTACTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2628758‑2628616 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCT  >  NC_000913/2996361‑2996510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.