| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,969,794 | 0 | A | C | 57.9% | 24.1 / 48.2 | 38 | G397G (GGT→GGG) | tap | methyl‑accepting protein IV |
| Reads supporting (aligned to +/- strand): ref base A (13/3); new base C (0/22); total (13/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.03e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-05 | |||||||||||
CACCGAACTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTT‑GATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCT > NC_000913/1969650‑1969942 | cACCGAACTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTT‑GATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGc > 2:794275/1‑150 (MQ=255) cTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTT‑GATCTCTTTTGCTGCCTGCGCGCTACGGCTGGAAAGATTGCGCACTTCACCTGCCACTAcc < 2:645047/150‑1 (MQ=255) tGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTT‑GATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGc > 1:812619/1‑151 (MQ=255) atatCGATCATGGTCGCGGCGGTTTTATCCACCAGTTTCGACCCCTGCGGGACACGATTGATGGCCTCTTCGATCAGCCCTTT‑GATCTCTTTTGCGGCCTGCGCGCTACGGCTGGAAAGTTTGCGCACTTCCCCTGCCACTACCGCaa < 1:272217/148‑1 (MQ=255) tatCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTT‑GATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAAcc > 2:937547/1‑151 (MQ=255) cGATCATGGTCGCGGCGGCTTTATTCACCAGTTTCGACCCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTT‑GATCTCTTTTGCTCCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCCCCTGCCACTACCGCAAAACCAc < 2:256701/150‑1 (MQ=255) cGATCATGGCCGCGGCGGCTTTATCCACCAGTTTCGACCCCTGCTGGACCCTATTGACTGCCTCTCCGTTCACTTTTTT‑GACCTCTTCTCCCCCCACCGCGCTCCCCCGGAAAAAATTCCGCCCTTCCCCTGCCACTACCGCAAAACCACg < 1:23973/151‑1 (MQ=255) aTCATGGACGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTT‑GATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGcc > 2:475702/1‑151 (MQ=255) tCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTT‑GATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGccc > 1:570009/1‑151 (MQ=255) gTTTTTCCCCAGTTTCGACCCCTGCTGGACACGTTTGACTGCCTCTTCGATCAGCCCTTT‑GATCTCTTTTGCTCCCTGCGCGCTCCGGCTGGCAAGATTGCGCACTTCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGcgcg < 2:624435/150‑1 (MQ=255) tttttCCCCATTTTCGACCCCTGCTGGCCACTATTGACGGACTCTTCGATCACCCCTTT‑GATCTTTTTTGCTCCCTGCGCGCTCCGGCTGGCAGGTTTGCGCCCTCCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGgcgc < 1:227153/145‑1 (MQ=255) ttATTCCCCTGTTTCAACCCCGGCTGGACACGATTGACTGACTCTTCGATCACCCCTTT‑GATCTTTTTTGCTCCCGGCGCGCTACGGCTGCAAAGATTGCGCACTTCCCCTGCCACTACCGCAAAACCACGCCCCTGctct < 1:518339/141‑1 (MQ=255) cACCAGTTCCGAACCCTGCTGGACACGTTTGACTGACTCTTCGATCACCCCTTT‑GATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATGCCGCACTCCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCg < 1:450148/149‑1 (MQ=255) aGTTTCGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTT‑GATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTcc > 2:58360/1‑150 (MQ=255) gctgGACATGATTGACTGACTCTTCGATCCCCCCTTT‑GATCTCTTTTGCTCCCGGCGCGCTGCGGCGGGAAGGATGGCGCCCTCCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCAtt < 2:869808/148‑1 (MQ=255) gggACACGTTTGACTGACTCTTCGATCAGCCTTTT‑GATCTTTTTTGCTCCCTGCGCGCTCCGGCTGGCAAGATTGCGCCCTTCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAggg < 2:944576/150‑1 (MQ=255) cGATTGACTGACTCTTCGATCAGCCCTTT‑GATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGa > 2:853391/1‑150 (MQ=255) ttgccggactCTCCGTCCAGCCCTTG‑GACCTTGTTGGCTGCCGGCGGGCGCGGGCGGGAAAGATTGCCCATTCCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGaa < 1:383707/142‑1 (MQ=16) ttttcgtccaccccTTT‑GATCTCTTTTGCTCCCTGCGCTCTACGGCTGCCAAGATTGCCCCCTTCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCt < 2:828953/137‑1 (MQ=25) ctctcggtccacccTTTGGATCTCTTTCGCTCCCTGCGCGCAGCGGCTGCCAATATTGCGCCCTTCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTc < 1:384697/137‑1 (MQ=21) ttataccaccctttt‑GATTTTTTTTGCTGCCGGCGCTTTAGGGCTGGAAAGTTTTCGCTCTTCCCCTGCCACTCCCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATAtt < 2:107591/129‑1 (MQ=21) tccGACCAGCCTTTT‑GTTCTCTTTTGCTCCCTGCGCGCTACGGCTGGAAAGATTGCCCACTTCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTc < 2:733470/143‑1 (MQ=25) cAGCCCTTT‑GATCTCTTTTGATGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCCGGGCCAGAATATTGGTCTGGAAAGCAAt > 2:790258/1‑150 (MQ=255) aGCCCTTT‑GATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTc > 2:853351/1‑151 (MQ=255) ccTTT‑GATCTCTTTTGCTCCCGGCGCGCTCGGGCGGGAAAGATTGCGCACTCCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAAtt < 2:86864/147‑1 (MQ=25) ttt‑GATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCg > 1:429967/1‑151 (MQ=255) aTCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATa > 1:701519/1‑150 (MQ=255) aTCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATa > 1:337645/1‑150 (MQ=255) cttgcGCGCGGCGGCGGCCAAAATTGCCCTCTCCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAAt < 2:120753/146‑1 (MQ=25) cctgcGCGCTCCGGCGGCCAAGATGGCGCACTTCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTc < 2:986278/151‑1 (MQ=255) ccggcgcgcTAGGGCTGGAAAGTTGGCCCCCTCCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATa < 1:379255/143‑1 (MQ=25) ccggccccttccGGGTGGCAAGTTCGCCCATTCCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAAt < 2:744418/137‑1 (MQ=25) cgcTCCGGCGGCCAAGTTTGCCCCCTTCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAAt < 2:71306/151‑1 (MQ=25) cgcTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAAt > 1:29903/1‑151 (MQ=255) tACGGCTGGAAAGATTGCGCCCTCCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAAtt < 2:687599/149‑1 (MQ=255) ggCTGGAAAGATTGCGCCCTCCCCCTGCCCCTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTg < 2:910284/151‑1 (MQ=255) aaGATTGCGCCTTTCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCt < 2:498961/150‑1 (MQ=255) cACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCt < 2:867327/150‑1 (MQ=255) | CACCGAACTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTT‑GATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCT > NC_000913/1969650‑1969942 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |