Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,288	0	T	G	43.6%	81.3 / 18.5	56	V528G (GTG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (3/28);  new base G (24/0);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.18e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGC  >  NC_000913/354141‑354437                                                                                                                                                     |                                                                                                                                                      ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGa                                                                                                                                                     <  1:32368/150‑1 (MQ=255)  gggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGaaa                                                                                                                                                   <  2:242848/151‑1 (MQ=255)      aTCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGTGAAAGATg                                                                                                                                               >  1:518005/1‑151 (MQ=255)        ccGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGGTGGGGGGGAAAATgcg                                                                                                                                             >  1:353082/1‑151 (MQ=255)            gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGC‑GGGTGGGGGGGAAGAAGGGCTg                                                                                                                                          >  1:572115/1‑150 (MQ=255)                       ttATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGGTGGGGGGGAAAATGGGCTGGAAggggggg                                                                                                                                >  1:240830/1‑145 (MQ=255)                         aTCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtgg                                                                                                                             <  1:517357/150‑1 (MQ=255)                              attATTCTCGGGCGCACTGACGATGTAATTAACGATGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGAGGAAGCTGCGCCGTAAAGGCAgggggcggcgg                                                                                                                       >  1:680846/2‑151 (MQ=255)                                 aTTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAACATGGGCTGAAAAGGGCggtgggggggg                                                                                                                       >  1:505083/1‑148 (MQ=255)                                    ctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGGAAAATGCGCTGAAAGGGCAGGGGGCGGGGGGGttt                                                                                                                  >  2:52533/1‑150 (MQ=255)                                      cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTTGGGGGGAAA‑ATGCGCTTGAAGGGGAgggggcggggggggtt                                                                                                                  >  1:750577/1‑143 (MQ=255)                                       gggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCa                                                                                                              <  1:667257/151‑1 (MQ=255)                                          cgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTc                                                                                                           <  1:207418/151‑1 (MQ=255)                                          cgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGGGGTTGTCAtt                                                                                                            >  2:58915/1‑150 (MQ=255)                                           gcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTcc                                                                                                          <  2:38174/151‑1 (MQ=255)                                               tGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGGTGCGCTGAAAGGGGAGGGGGGGGTGGCGTTTGTCATTTCGa                                                                                                        >  2:927933/1‑149 (MQ=255)                                                gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGaa                                                                                                       <  1:469288/149‑1 (MQ=255)                                                gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAag                                                                                                     <  1:580585/151‑1 (MQ=255)                                                gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGGGCTGAAAGGGGAGGGGGGGGGGGGGGTTGTCATTCCGgaa                                                                                                      >  1:314400/1‑150 (MQ=255)                                                 aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGGTTGTCATTCCGaa                                                                                                       >  2:123679/1‑148 (MQ=255)                                                      gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGCGCTGGAAGGGGAGGGGGCGGGGGGGTTTGTTATTCCGGAagagaggg                                                                                               >  1:584950/1‑149 (MQ=255)                                                      gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGCGCTGGAAGGGCCGGGGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCg                                                                                               >  2:582475/1‑151 (MQ=255)                                                      gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGGTGGGGGGAAAGATGCGCTGAAAGGGGCGGGGGCGGGGGGGGTTGTCATTCCGGAagagag                                                                                                 >  2:679506/1‑149 (MQ=255)                                                          ttAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGTGGCGGTTGTCATTCCGAAAGAGAGCg                                                                                               >  2:886969/1‑147 (MQ=255)                                                            aaCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGGTGGGGGGGAAGATGCGCTGGAAGGGGAGGGGGGGGGGGGGTTTGTCATTTCGAAagagaggg                                                                                               >  1:95855/1‑143 (MQ=255)                                                                  gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCCGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGaa                                                                                    <  1:658709/150‑1 (MQ=255)                                                                  gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGaa                                                                                    <  1:781423/150‑1 (MQ=255)                                                                   ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGGGCTGAAAGGGGAGGGGGCGGGGGGGGTTGTCATTCCGAAAAAGAGGGGCAGTCTg                                                                                       >  2:210035/1‑146 (MQ=255)                                                                   ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGGGTTTGTCATTTCCAAAGAGAGCGACAGGCTgg                                                                                      >  2:49616/1‑147 (MQ=255)                                                                    cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAt                                                                                 <  2:733013/151‑1 (MQ=255)                                                                    cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGGAAGAAGGGCTGAAAGGGCAGGTGGGGGGGGGGGTTGGCATTCCGAAAGAGAGCGAAAATCt                                                                                        >  2:642444/1‑144 (MQ=255)                                                                      ggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGGGGGGGGGGTTGTCATTTCGAAAGAGAGCGACAGTCTGGAAGAACg                                                                               >  2:928341/1‑151 (MQ=255)                                                                       gCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGt                                                                              <  1:716854/151‑1 (MQ=255)                                                                            ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtgt                                                                         <  1:160120/151‑1 (MQ=255)                                                                                ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTgg                                                                       <  1:600210/149‑1 (MQ=255)                                                                                    aCGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACt                                                                 <  2:788634/151‑1 (MQ=255)                                                                                      gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTca                                                               <  1:387215/151‑2 (MQ=255)                                                                                      gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCg                                                               <  2:345563/151‑1 (MQ=255)                                                                                       cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCg                                                               <  1:237861/150‑1 (MQ=255)                                                                                           agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAag                                                           <  1:899655/150‑1 (MQ=255)                                                                                           agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAAATGCGCTGGAAGGGGCGGGGGGGGGGGGGGTTGTCATTCCGAAAGAGAGCGGCAGTCTGGGAGATCGTGGTGTGGCGCACTCGCa                                                             >  1:926170/1‑148 (MQ=255)                                                                                            gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAagag                                                         <  1:337172/151‑1 (MQ=255)                                                                                              ttGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTTGGGGGAAAGATGCGCTGAAAAGGCAGGGGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAaga                                                          >  2:74250/1‑148 (MQ=255)                                                                                                        tATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTAt                                              >  2:994996/1‑150 (MQ=255)                                                                                                               aGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtgg                                      <  1:177916/151‑1 (MQ=255)                                                                                                                         gCGTTGCCGAAGTGGCGGT‑GGGTGGGGTGGAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGGTTATGGCGCTGGGGGGCAGcc                             >  1:48032/1‑150 (MQ=255)                                                                                                                          cGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAg                           <  2:926205/151‑1 (MQ=255)                                                                                                                          cGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCTGAAAGGGAAGGGGGCGGGGGGGTTTGTCATTTCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGGGCCCTCGCAAGAGAAGGGGATTATGGCGCTGGGGGACAGcc                             >  2:997061/1‑149 (MQ=255)                                                                                                                           gTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCTGTAAGGGGAGGGGGCGGGGGGGGTTTTCATTCCGAAAGAGAGCGACAGTCTTGAAGATCGTGGTGGGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggggggacgcc                             >  1:873869/1‑142 (MQ=255)                                                                                                                             tGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGAt                         <  2:434595/150‑1 (MQ=255)                                                                                                                             tGCCGAAGTGGCGGT‑GGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGGCAGTCTGGAAGATCGTGATGGGGCGCACTCGCAAGAGAAGGGGGATATGGCGCTGGGGGACAGCCAGAtt                        >  1:275291/1‑151 (MQ=255)                                                                                                                               ccGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTgg                      <  1:886936/151‑1 (MQ=255)                                                                                                                                      ggCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACtt                <  1:140696/150‑1 (MQ=255)                                                                                                                                                tGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGccc       <  2:494547/150‑1 (MQ=255)                                                                                                                                                 ggggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCg      <  2:546782/150‑1 (MQ=255)                                                                                                                                                    gTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgcgc  <  1:651259/151‑1 (MQ=255)                                                                                                                                                     tGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCTGgcgc  >  1:11411/1‑150 (MQ=255)                                                                                                                                                     tGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgcgc  >  1:680595/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                      GGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGC  >  NC_000913/354141‑354437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑