Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,261,586	A→C	intergenic (+280/‑83)	yjbM → / → dusA	uncharacterized protein/tRNA‑dihydrouridine synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,261,586	0	A	C	100.0%	307.0 / NA	79	intergenic (+280/‑83)	yjbM/dusA	uncharacterized protein/tRNA‑dihydrouridine synthase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (33/46);  total (33/46)

AACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGCGCAATGCCTGAAAAAACTGACG  >  NC_000913/4261444‑4261732                                                                                                                                               |                                                                                                                                                   aaCCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCt                                                                                                                                             >  1:13908/1‑150 (MQ=255)   ccTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTAtt                                                                                                                                          <  2:613961/151‑1 (MQ=255)    cTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTAtt                                                                                                                                          <  2:686046/150‑1 (MQ=255)    cTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTg                                                                                                                                         >  2:1001720/1‑151 (MQ=255)     tGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTg                                                                                                                                         >  2:609885/1‑150 (MQ=255)      ggATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGa                                                                                                                                        <  1:427599/150‑1 (MQ=255)        aTTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGcc                                                                                                                                     >  1:826198/1‑151 (MQ=255)           gTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGcctcc                                                                                                                                  >  2:488525/1‑151 (MQ=255)              tATCATTGGCCTTAACAAAGTTAACGGCTAATAAGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTg                                                                                                                                >  1:709888/1‑150 (MQ=255)               aTCATTGGCCTTAACAAAGTTAACGGCTAATAAGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGa                                                                                                                               <  1:567381/150‑1 (MQ=255)               aTCATTGGCCTTAACAAAGTTAACGGCTAATAAGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGa                                                                                                                               >  1:34779/1‑150 (MQ=255)                 cATTGGCCTTAACAAAGTTAACGGCTAATAAGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATa                                                                                                                            <  1:540914/151‑1 (MQ=255)                   ttGGCCTTAACAAAGTTAACGGCTAATAAGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGt                                                                                                                          >  2:122869/1‑151 (MQ=255)                      gCCTTAACAAAGTTAACGGCTAATAAGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGAt                                                                                                                       <  1:165284/151‑1 (MQ=255)                           aaCAAAGTTAACGGCTAATAAGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTg                                                                                                                   <  2:800709/150‑1 (MQ=255)                           aaCAAAGTTAACGGCTAATAAGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGa                                                                                                                  <  2:673843/151‑1 (MQ=255)                           aaCAAAGTTAACGGCTAATAAGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGa                                                                                                                  <  1:88994/151‑1 (MQ=255)                              aaaGTTAACGGCTAATAAGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAata                                                                                                               <  1:32468/151‑1 (MQ=255)                               aaGTTAACGTCTAATAAGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAata                                                                                                               >  1:138758/1‑150 (MQ=255)                                aGTTAACGGCTAATAAGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataac                                                                                                             >  1:705793/1‑151 (MQ=255)                                    aaCGGCTAATAAGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAataacata                                                                                                          <  1:653821/150‑1 (MQ=255)                                           aataaGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATg                                                                                                  <  1:611662/151‑1 (MQ=255)                                            ataaGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGa                                                                                                 <  1:156818/151‑1 (MQ=255)                                            ataaGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGa                                                                                                 <  2:943537/151‑1 (MQ=255)                                              aaGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTGCTGAATAGTGATGCTGAATAACATAACCCCATGata                                                                                               <  1:684345/151‑1 (MQ=255)                                               aGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGatat                                                                                              <  2:602890/151‑1 (MQ=255)                                                gATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGatat                                                                                              >  1:266077/1‑150 (MQ=255)                                                  ttattTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATc                                                                                           >  1:763507/1‑151 (MQ=255)                                                       tCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATa                                                                                       <  1:772172/150‑1 (MQ=255)                                                       tCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATa                                                                                       >  2:296225/1‑150 (MQ=255)                                                        ccATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATaaa                                                                                     <  2:819641/151‑1 (MQ=255)                                                        ccATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATaa                                                                                      >  2:127863/1‑150 (MQ=255)                                                            cACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAaataa                                                                                 <  1:670988/151‑1 (MQ=255)                                                            cACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAaataa                                                                                 <  2:48612/151‑1 (MQ=255)                                                            cACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAaata                                                                                  <  1:273063/150‑1 (MQ=255)                                                                tCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAAtct                                                                              <  2:364947/150‑1 (MQ=255)                                                                  gTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAACATAAtctc                                                                             >  1:1014924/1‑149 (MQ=255)                                                                    gagaGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTAc                                                                          <  2:703852/150‑1 (MQ=255)                                                                     agagCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACAt                                                                        <  1:127861/151‑1 (MQ=255)                                                                      gagCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACAtt                                                                       >  1:367117/1‑151 (MQ=255)                                                                          ttCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGa                                                                    >  1:673883/1‑150 (MQ=255)                                                                            cATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGaaa                                                                  <  1:691964/150‑1 (MQ=255)                                                                            cATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGaaa                                                                  <  1:779639/150‑1 (MQ=255)                                                                               gCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATg                                                               <  1:457085/150‑1 (MQ=255)                                                                                  ttGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACg                                                           >  1:699891/1‑151 (MQ=255)                                                                                   tGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCAc                                                            <  2:47273/149‑1 (MQ=255)                                                                                      gagGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTaa                                                       <  2:702121/151‑1 (MQ=255)                                                                                      gagGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTaa                                                       <  1:630187/151‑1 (MQ=255)                                                                                      gagGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTa                                                        >  2:76556/1‑150 (MQ=255)                                                                                            ctcAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTg                                                  >  2:562315/1‑150 (MQ=255)                                                                                              cAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGa                                                 <  1:866533/149‑1 (MQ=255)                                                                                               aaTTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAAt                                              <  2:572522/151‑1 (MQ=255)                                                                                                       tttGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCaaaaa                                       >  2:844050/1‑150 (MQ=255)                                                                                                       tttGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCaaaaa                                       <  2:767432/150‑1 (MQ=255)                                                                                                        ttGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCaaaaaa                                      <  2:163814/150‑1 (MQ=255)                                                                                                             aTGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCaacc                                <  1:471391/151‑1 (MQ=255)                                                                                                             aTGAGACAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCaacc                                <  1:336818/151‑1 (MQ=255)                                                                                                               gagaCAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCaacca                               >  2:129645/1‑150 (MQ=255)                                                                                                                agaCAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCaaccaaa                             <  2:261751/151‑1 (MQ=255)                                                                                                                  aCAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAATAATCaaccaaacc                           <  1:879761/151‑1 (MQ=255)                                                                                                                  aCAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCaaccaaacc                           >  1:861894/1‑151 (MQ=255)                                                                                                                   cAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCaaccaaacc                           >  1:512340/1‑150 (MQ=255)                                                                                                                   cAGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCaaccaaacc                           >  1:147782/1‑150 (MQ=255)                                                                                                                    aGGCGCTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCaaccaaacc                           >  1:502569/1‑149 (MQ=255)                                                                                                                      gcgcTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCaaaaa                                       <  1:773343/135‑1 (MQ=255)                                                                                                                      gcgcTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCaaaaa                                       >  2:773368/1‑135 (MQ=255)                                                                                                                      gcgcTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAACCCAgcg                       <  2:454708/151‑1 (MQ=255)                                                                                                                      gcgcTTCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAgcg                       <  1:142989/151‑1 (MQ=255)                                                                                                                          ttCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGCGCAAt                   >  1:687879/1‑151 (MQ=255)                                                                                                                           tCCTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCTCGGTAATTCTGAAATGCAAAAAATCAACCAAACCATCGCaa                    <  1:402548/149‑1 (MQ=255)                                                                                                                             cTGTTGTTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGCGCAATGcc                >  2:98389/1‑151 (MQ=255)                                                                                                                              tgttgtTATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGCGCAATGCCt               >  1:796829/1‑151 (MQ=255)                                                                                                                               gttgttATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGCGCAATGCCTg              >  2:421019/1‑151 (MQ=255)                                                                                                                               gttgttATGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGCGCAATGCCTg              <  1:84693/151‑1 (MQ=255)                                                                                                                                     aTGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGCGCAATGCCTGaaaaa         >  2:280411/1‑150 (MQ=255)                                                                                                                                      tGGTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGCGCAATGCCTGAAAAAAc       >  2:407494/1‑151 (MQ=255)                                                                                                                                       ggTATAGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGCGCAATGCCTGAAAAAACt      <  2:733440/151‑1 (MQ=255)                                                                                                                                          ataGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGCGCAATGCCTGAAAAAACTGa    <  2:861924/150‑1 (MQ=255)                                                                                                                                            aGTCCCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTTCATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAATCCAGCGCAATGCCTGAAAAAACTGACg  <  2:496153/150‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                   AACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGATTATTTCCATCACTTCGTGAGAGCTTCATGCCTTGAGAGGATCTCAATTTTCTTTTGCAATGAGACAGGCGCTTCCTGTTGTTATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGCGCAATGCCTGAAAAAACTGACG  >  NC_000913/4261444‑4261732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑