Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,921,177	0	A	C	51.8%	33.9 / 42.7	27	G308G (GGT→GGG)	gudP	putative D‑glucarate transporter
Reads supporting (aligned to +/- strand):  ref base A (13/0);  new base C (0/14);  total (13/14)
Fisher's exact test for biased strand distribution p-value = 4.99e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.03e-05

CAGCGCCATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGTACTGACCGAT  >  NC_000913/2921027‑2921326                                                                                                                                                       |                                                                                                                                                      cAGCGCCATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATccca                                                                                                                                                       <  1:1290259/151‑1 (MQ=255)             gCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACAccg                                                                                                                                           >  1:1222268/1‑151 (MQ=255)                 atgatCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACAccgccg                                                                                                                                        >  2:2387637/1‑150 (MQ=255)                         ccACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGGGCGGCGCATCAGCCAGTCGAAAATAATCCCCCCCAGCACACCGCCGATAaaaccg                                                                                                                               <  2:1962861/151‑1 (MQ=255)                            cacAACGTGGACGTATTGGCAAAACACCACCCCAACCGACAGCAACACCCCCATTCCGATCGGTGTTTTACGCGCAATGTTCAGCGACCCCGGGCGGCGCACCCCCAAGTCGGAAATAACCCCCCCCAGCACACCGCCGATAaaaccg                                                                                                                               <  1:2267563/146‑1 (MQ=255)                                aCGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAaaaccgcaaaccg                                                                                                                        >  2:2419188/1‑151 (MQ=255)                                 cGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAaaaccgcaaaccgc                                                                                                                       >  2:2312169/1‑151 (MQ=255)                                                  aaCACCATCACCATCGCCAGCAACATGCCCAGTACGATCGGTGTTGGACGCGCAAGGTTCAGCGATCCCGTGCGGCACAACAGCCAGTCGGAAATAATCCCACCCAGCACACCGCAGATAAAACCGCAAACCGCCGGAACGGAAGCcaca                                                                                                       >  1:610680/1‑150 (MQ=255)                                                            ccaccGAAACCAACATCCCCATGCCGATCGGTTTTTTACGCGCATTGTCCGGCGCCCCCGTGCGGGGAACCAGCCGGTGGAAAATATCCCCCCCCGCCCCGCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCt                                                                                              <  1:334922/149‑1 (MQ=255)                                                                    aGCAACATCCCAATTACGATGGGGGTTTTACGCGAAATGTCAAGCGACCCCGTGCGGCGCATCAGCCAGTCGGAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATc                                                                                     <  2:2518455/150‑1 (MQ=255)                                                                             cccTTTACGATCGGTGTTTCCCCCGCAATGTCCAGCGACCCCGTGCGGCCCATCAGCCAGTCGAAAAAAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCcgc                                                                           <  1:3460209/151‑1 (MQ=255)                                                                              ccATTCCGATCGGTTTTTTCCGCGCAATGTGCAGCGCCCCCGTGCGGCGCATCAGCCAGTCGGAAATAACCCCCCCCACCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATccc                                                                             <  1:671840/148‑1 (MQ=255)                                                                                      aTCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGc                                                                   >  2:464705/1‑150 (MQ=255)                                                                                        cGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCAcc                                                                >  2:141583/1‑151 (MQ=255)                                                                                         ggTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCAcc                                                                >  1:1139724/1‑150 (MQ=255)                                                                                              tttACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATa                                                           >  1:1502395/1‑150 (MQ=255)                                                                                                    cgaaaTGTTCAGCGACCCCGTGCGGCGCACCACCCGGCCGAAAAAAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCg                                                      <  2:2050843/146‑1 (MQ=255)                                                                                                             cAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGt                                            >  2:2685645/1‑150 (MQ=255)                                                                                                               gCGATCCGGTGGGGCGCACCGCCCAGTCGGAAAAACCCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAAc                                                        <  1:3987731/136‑1 (MQ=255)                                                                                                               gCGACCCCGGGCGGCGCATCGGCCAGTCGAAAATAATCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGtaat                                         <  2:3846468/151‑1 (MQ=255)                                                                                                                 gATCCCGGGCGTCGCATCAGCCAGTCGGAAATAATACCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCCGACTCGACATCCCGCGTGCCTGCACCCGATAAACCGGGAACCACGtaata                                        >  1:3134600/1‑150 (MQ=255)                                                                                                                  ctCCCGTGCGCCGCACCAGCCAGTCAGAAATAACCCCCCCCAGCCCCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGta                                           <  1:914104/145‑1 (MQ=255)                                                                                                                    cccGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGtaataa                                       >  1:2512105/1‑148 (MQ=255)                                                                                                                                   aGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGt                     >  2:435307/1‑151 (MQ=255)                                                                                                                                     ccggccgaaaaTAATCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGGCATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGtt                    <  2:770149/142‑1 (MQ=255)                                                                                                                                     ccAGTCGAAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGt                     <  2:2440210/149‑1 (MQ=255)                                                                                                                                     ccAGTCGAAAATAACCCCCCCCACCACCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTg                   <  1:2311949/151‑1 (MQ=255)                                                                                                                                     caattCGAAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGtt                    <  1:2713128/146‑1 (MQ=255)                                                                                                                                                   ccccccccaGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGTACTg        <  1:1174200/147‑1 (MQ=255)                                                                                                                                                       acccaGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGTACTGACCGat  >  1:1087657/1‑150 (MQ=255)                                                                                                                                                       |                                                                                                                                                      CAGCGCCATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGTACTGACCGAT  >  NC_000913/2921027‑2921326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑