Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,508,6620TA41.7% 57.4 / 10.5 37E384D (GAA→GAT) yhfWphosphopentomutase‑related metalloenzyme superfamily protein
Reads supporting (aligned to +/- strand):  ref base T (3/18);  new base A (15/0);  total (18/19)
Fisher's exact test for biased strand distribution p-value = 2.93e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCATCA  >  NC_000913/3508523‑3508810
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ttAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGCGGCGCGCGGGAAAAATCacaaacag                                                                                                                                            >  2:3146431/1‑148 (MQ=255)
  aaaGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGGGGGAAAAATtaca                                                                                                                                                 >  1:2061888/1‑143 (MQ=255)
  aaaGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTa                                                                                                                                          <  2:2809424/150‑1 (MQ=255)
  aaaGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTa                                                                                                                                          <  2:2961787/150‑1 (MQ=255)
              aTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATTACACACGGGAGCCCCcac                                                                                                                                  >  2:1765464/1‑146 (MQ=255)
                  atatGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATTACACACGGGAGCCCCCCCATCAGaaa                                                                                                                          >  1:1297896/1‑150 (MQ=255)
                       cAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTTGGGCGCGGGAAAAATCACACACGGGAGCCCCCCCATCAGAAAACgtgg                                                                                                                    >  2:130709/1‑151 (MQ=255)
                        aGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCgtggt                                                                                                                   <  2:788830/151‑1 (MQ=255)
                               cATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACACACGGTAGCCCCCCCATCCGAAAGCgggggg                                                                                                                  >  2:2394708/1‑143 (MQ=255)
                                     cGAGAGTGTAACGCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGAGGCGGTGGAGCGCGGAAAAAGTCACACACGGTTGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGc                                                                                                      <  1:3969403/151‑1 (MQ=255)
                                          gtgtAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGt                                                                                                 <  2:2152598/151‑1 (MQ=255)
                                            gtAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTa                                                                                                <  2:3159835/150‑1 (MQ=255)
                                                cccGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATCACACACGGGAGCCCCCCCCTCAGAAAACGGGGGGGGGCCACCCAGCTGCGGAGCg                                                                                             >  1:3119801/1‑149 (MQ=255)
                                                  cGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGGGCGCCCACCCAGCTGCGGAGCGatc                                                                                          >  2:3731993/1‑150 (MQ=255)
                                                  cGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATTACACACGGTAGCCCCCCCATCAAAAAGAGGGGGGGGCACACCCAGCTGCGTAGCGgtc                                                                                          >  2:502656/1‑150 (MQ=255)
                                                   gTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATTACACACGGGAACCCCCACATCAGAAAGCGTGGGGCGCACACCCAGCTGCGGAGCGatca                                                                                         >  1:3505883/1‑150 (MQ=255)
                                                        aTCAAAACTCATAGGGTGTCTCCTGCAACCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGTCGGTGGTTTTTTTAAAAATTCACACGCGGTCCCCCCCTCATCAGAAAGGGTGGCCCGCACTCCCCGCCGCGTAGCTTTCATCACtc                                                                                   <  1:1592882/151‑2 (MQ=255)
                                                           aaaaCTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCt                                                                                <  1:152259/151‑1 (MQ=255)
                                                              aCTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACACACGGTAGCCCCCCCATCAGAAAACGTGGTGCGCACACCCAGCTGCGGAGCGATCATCCCCTGCt                                                                                >  2:3807924/1‑148 (MQ=255)
                                                               cTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATTACAAACGGGAGCCCCCCCAACCGAAAGCGTGGGGCGCACACCCCGCCGCGGAGCGATCATCCCCTTCTGAt                                                                             >  2:1775509/1‑150 (MQ=255)
                                                                 cATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATaaa                                                                          <  1:1642210/151‑1 (MQ=255)
                                                                  aTAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAAc                                                                         <  2:212991/151‑1 (MQ=255)
                                                                             ccTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGc                                                               <  1:3219491/150‑1 (MQ=255)
                                                                                gCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCAAACACGGTAGCCCCCACATCAGAAAACGGGGGGGGCCCACCCAGCAGCGGAGCGGTCATCCCCTTCTGATAAAACAGCACCgggccc                                                            >  1:3181047/1‑146 (MQ=255)
                                                                                   aaCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACtt                                                        <  2:1529465/151‑1 (MQ=255)
                                                                                      cGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTcgc                                                     <  2:865174/151‑1 (MQ=255)
                                                                                                 aagaaaagaGCGACCATTTTGTGGCGGTGGGGCGCGGAAAAAATCACACAAGGGAGCCCCCCCATCAGAAAACGGGGTGGGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAAACAAACCTGGCACCACTTCGCGGGGAtggtgg                                          >  1:1095781/1‑151 (MQ=255)
                                                                                                  agaaaagaGCGACCATTTTGTGGCGGTGGGGCCCGGGAAAAATCACACACGGGAGCCCCCCCATCAGAAAACGTGGTGCGCACACCGAGCTGCGTAGCGGTCATCCCCTGCTGATAAAACAACACTCGCACCACCTCGCGGGGAtggggg                                          >  2:4076584/1‑150 (MQ=255)
                                                                                                               cATTTTGTGGCGGTGGCGCGCGGAAAAAATCACACACGGTAGGCCCCCCATTCGAAAGCGGGGGGCGCACACCCAGCCGCGTAGCGAACACTCCCTGCCGCTAAAACAGCACCTGCACCACCTCGCGGGGATGGTGGATGGGAcccaagg                             >  1:804702/1‑145 (MQ=255)
                                                                                                                   ttGTGGCGGTGGGGCGCGGAAAAATTCAAACAACGGAGCCCCCACATCATAAAGAGGGGTGCGCACACCGAGCTGCGTAGAGAACATACCTTGCTGGTAAAACAACACTGGCACCCCCTCGCGGGTAAGGTGGGGGTGACCAAATGTCgg                         >  1:1310929/1‑150 (MQ=255)
                                                                                                                         cggtggcgCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTg                  <  2:305311/151‑1 (MQ=255)
                                                                                                                                  gcgGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATc          <  1:2047400/150‑1 (MQ=255)
                                                                                                                                   cgGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCg        <  1:1775262/151‑1 (MQ=255)
                                                                                                                                    ggAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTc                                                       <  1:3968443/103‑1 (MQ=255)
                                                                                                                                    ggAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTc                                                       >  2:3968640/1‑103 (MQ=255)
                                                                                                                                        aaaTTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCATc   <  2:3120012/151‑1 (MQ=255)
                                                                                                                                         aaTTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCATCa  <  2:1312977/151‑1 (MQ=255)
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TTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCATCA  >  NC_000913/3508523‑3508810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑