Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,336,901	0	T	A	47.6%	12.8 / 10.8	21	Y44F (TAT→TTT)	adiC	arginine:agmatine antiporter
Reads supporting (aligned to +/- strand):  ref base T (7/4);  new base A (0/10);  total (7/14)
Fisher's exact test for biased strand distribution p-value = 3.87e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

CAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAG  >  NC_000913/4336751‑4337049                                                                                                                                                       |                                                                                                                                                     cagTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAAccatccat                                                                                                                                                      <  2:2338128/151‑1 (MQ=255)           cGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCaa                                                                                                                                             >  1:1622762/1‑150 (MQ=255)            gTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATc                                                                                                                                           <  2:3155285/151‑1 (MQ=255)                    gATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCTATAATCGTCACCAACCATCCATAAATGACAATCCCGCCa                                                                                                                                     <  2:709634/149‑1 (MQ=255)                            agaAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGACCCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATCCCATCGAGACCCCCAGTGCACCGATAATCGTCACCACCCATCCATAAATGGCAATCCCGCCAGTAGAGGcc                                                                                                                            <  2:4033238/150‑1 (MQ=255)                            acaaaCGGGCCAAGGCAGCGGGGGGCGGAGGCGAAAAAACCCCAAGGACTGGGGGCAGGGACCGCCTTTTGGGCGTTTCCAATGGAGGGCCCCGGGGCCCCGAAAATCGTCACCACCCATCAAAAAAGGCAAATCCCGCCAGTAGAGGCCa                                                                                                                           <  1:2951824/149‑1 (MQ=255)                                            agcGGCGGGCGGAAGCGGAAGAACCACCAGGGCTTGGGTCGAGGGACGGCATTTTGGCGGATACCATCGAGAGCCCCAGGGCACCGATAATCGTCACCAACCCTCCATAAATGGCAATCCCGCCAGTAAAGGCCAGGGTTGCAGGTaa                                                                                                              >  1:1156123/1‑148 (MQ=255)                                               ggggggcgTAGGCGTAAGAACCCCCGGGACTTGGGTCGAGGAACGACATTTTGGCGTATCCCATCGAGACCCCCAGTGCCCCGATAACCGTCACCACCCATCCAAAAAGGGCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAg                                                                                                           <  2:1649205/145‑1 (MQ=255)                                               ggcgggcgTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGaa                                                                                                         >  1:3216747/1‑150 (MQ=255)                                               ggcgggcgTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGaa                                                                                                         >  1:863916/1‑150 (MQ=255)                                                   ggcgTAAGCGTAAGACCCCCCAGGACTTGGGTCGAGGAACGACATTTGGGCGTATCCCATCGAGACCCCCAGTGCCCCGATAATCGTCCCCACCCACCCAAAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAaca                                                                                                    <  2:3586472/151‑1 (MQ=255)                                                         agcgtaagAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGAc                                                                                              >  1:2125114/1‑151 (MQ=255)                                                              aagAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATCCCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCAAAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGAccc                                                                                            <  2:3077859/148‑1 (MQ=255)                                                                   cccccaGGACTTGGGTCGAGAAACGACTTTTTGGCTTATCCCATCGAGAGCCCCAGTGCACCGATAATCGTCCCCACCCATCCAAAAATGGAAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCataat                                                                                      <  2:3512609/146‑1 (MQ=255)                                                                                        aaCGACTTTTTGGCGTATACCACCGAGCCCCCCAGGGCCCCAAAAATCGTCCCAACCCACCAAAAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTg                                                                <  1:3530218/150‑1 (MQ=255)                                                                                                   ggCGTATACCATCGAGAGCCCCAGTGCCCCGATAATCGTCACCAACCACCAAAAAAGGCCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAg                                                    <  2:1065154/151‑1 (MQ=255)                                                                                                     cGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGcc                                                  >  1:717612/1‑151 (MQ=255)                                                                                                           ccAGCGAGAGCCCCAGTGCCCCGATAATCGTCCCCAACCACCCAAAAATGGCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACttt                                            <  1:1578225/151‑1 (MQ=255)                                                                                                                 agagCCCCAGTGCACCGATAATCGTCACCACCCATCCAAAAATGGCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagc                                      <  1:1162417/151‑1 (MQ=255)                                                                                                                     ccccAGTGCACCGATAATCGTCCCCACCCACCCAAAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagcat                                    <  1:1071812/149‑1 (MQ=255)                                                                                                                     ccccAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGAAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagcatc                                   >  1:3518301/1‑150 (MQ=255)                                                                                                                                            ccaactaTCAATAAATAACATTCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATGCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACt           <  1:3447786/151‑1 (MQ=255)                                                                                                                                                ccacccatAAATGGAAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTg       <  2:2752756/151‑1 (MQ=255)                                                                                                                                                      atAAAGGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAg  <  2:1490591/150‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                     CAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAG  >  NC_000913/4336751‑4337049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑