Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,338,117 (A)8→7 intergenic (‑188/+137) adiY ← / ← adiA adi system transcriptional activator/arginine decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,338,1100A.99.1% 556.6 / ‑3.8 115intergenic (‑181/+144)adiY/adiAadi system transcriptional activator/arginine decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base . (71/43);  total (71/44)
Fisher's exact test for biased strand distribution p-value = 3.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

GTTCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTGG  >  NC_000913/4337961‑4338253
                                                                                                                                                     |                                                                                                                                               
gTTCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCaa                                                                                                                                                <  2:313489/151‑1 (MQ=255)
gTTCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCaa                                                                                                                                                <  3:1057746/151‑1 (MQ=255)
 ttCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCaa                                                                                                                                                >  4:337654/1‑150 (MQ=255)
  tctcGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCaaa                                                                                                                                               <  3:998873/150‑1 (MQ=255)
  tctcGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCaaa                                                                                                                                               <  2:833089/150‑1 (MQ=255)
    tcGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCaaaaa                                                                                                                                             >  2:240714/1‑150 (MQ=255)
    tcGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCaaaaa                                                                                                                                             >  2:482423/1‑150 (MQ=255)
     cGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCaaaaaaa                                                                                                                                           <  3:32933/151‑1 (MQ=255)
       cTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCaaaaaaagc                                                                                                                                         >  3:329449/1‑149 (MQ=255)
        tAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCaaaaaaagcg                                                                                                                                        >  3:1395400/1‑148 (MQ=255)
         aaagcaaagcGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCaaaaaaagcgc                                                                                                                                       <  2:954683/151‑5 (MQ=255)
         aaagcaaagcGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCaaaaaaagcg                                                                                                                                        <  3:1111756/150‑4 (MQ=255)
           agcaaagcGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCgg                                                                                                                                    <  2:328692/151‑1 (MQ=255)
             caaagcGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCgggg                                                                                                                                  <  2:937453/151‑1 (MQ=255)
               aagcGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTATTTTCTCATGTACATATTTTTTTACAGGTTAAATAACAGGCAAAAAAAGCGCGGGGTg                                                                                                                                >  2:483961/1‑151 (MQ=255)
                   gATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGc                                                                                                                            >  3:962396/1‑151 (MQ=255)
                      aCCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCcgc                                                                                                                         <  2:789101/151‑1 (MQ=255)
                       ccGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCcgcg                                                                                                                        >  2:1383044/1‑151 (MQ=255)
                          ggAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCa                                                                                                                      >  3:630527/1‑150 (MQ=255)
                          ggAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCa                                                                                                                      >  2:1060694/1‑150 (MQ=255)
                            aaaTGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCaaa                                                                                                                    >  2:1141131/1‑150 (MQ=255)
                            aaaTGGTTATATAATTATATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATTTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCaaaa                                                                                                                   <  2:1102107/151‑1 (MQ=255)
                             aaTGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCaaaaa                                                                                                                  <  2:918105/151‑1 (MQ=255)
                               tGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAg                                                                                                                 >  3:523910/1‑150 (MQ=255)
                               tGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGt                                                                                                                >  2:948932/1‑151 (MQ=255)
                                ggTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGt                                                                                                                >  3:259101/1‑150 (MQ=255)
                                   tatataATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATg                                                                                                             >  3:890345/1‑150 (MQ=255)
                                   tatataATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATg                                                                                                             >  2:560826/1‑150 (MQ=255)
                                        aaTTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACAttt                                                                                                       >  3:1093223/1‑151 (MQ=255)
                                        aaTTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACAttt                                                                                                       <  3:458165/151‑1 (MQ=255)
                                          ttGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACAttttt                                                                                                     >  3:861622/1‑151 (MQ=255)
                                          ttGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACAttttt                                                                                                     >  2:580289/1‑151 (MQ=255)
                                              atgatgAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTtttgttt                                                                                                 >  2:1160701/1‑151 (MQ=255)
                                              atgatgAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTtttgttt                                                                                                 <  2:307439/151‑1 (MQ=255)
                                              atgatgAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTtttgtt                                                                                                  <  3:1326679/150‑1 (MQ=255)
                                                gatgaATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTtttgtttg                                                                                                <  2:1246066/150‑1 (MQ=255)
                                                 atgaATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTgc                                                                                               >  3:1249909/1‑150 (MQ=255)
                                                       ataAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAAtt                                                                                        >  4:420373/1‑151 (MQ=255)
                                                         aaaCATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAAtttt                                                                                      >  1:222801/1‑151 (MQ=255)
                                                          aaCATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAAttttt                                                                                     >  3:410424/1‑151 (MQ=255)
                                                          aaCATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAAttttt                                                                                     <  2:38061/151‑1 (MQ=255)
                                                            cATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTAt                                                                                   >  2:898011/1‑151 (MQ=255)
                                                             aTGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTAt                                                                                   <  3:268144/150‑1 (MQ=255)
                                                               gCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGa                                                                                 >  3:365988/1‑150 (MQ=255)
                                                                   aaaaaTAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAAc                                                                             <  3:47497/150‑1 (MQ=255)
                                                                   aaaaaTAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACg                                                                            >  3:706264/1‑151 (MQ=255)
                                                                    aaaaTAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGt                                                                           >  2:347255/1‑151 (MQ=255)
                                                                     aaaTAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGTTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGtt                                                                          <  3:876023/151‑1 (MQ=255)
                                                                     aaaTAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGtt                                                                          <  2:802552/151‑1 (MQ=255)
                                                                     aaaTAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGtt                                                                          <  3:478699/151‑1 (MQ=255)
                                                                           cGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCAt                                                                     >  3:350390/1‑150 (MQ=255)
                                                                           cGAGTTTTACTAATAGTACAAATTTTTAAAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATa                                                                    <  3:498372/151‑1 (MQ=255)
                                                                             aGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACt                                                                  <  3:483995/151‑1 (MQ=255)
                                                                              gTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTc                                                                 <  3:552264/151‑1 (MQ=255)
                                                                                tttACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCa                                                                >  2:885133/1‑150 (MQ=255)
                                                                                  tACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCaaa                                                              >  3:1022432/1‑150 (MQ=255)
                                                                                  tACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCaaa                                                              >  2:395366/1‑150 (MQ=255)
                                                                                  tACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCaaa                                                              >  2:1232818/1‑150 (MQ=255)
                                                                                  tACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCaaa                                                              >  3:1298700/1‑150 (MQ=255)
                                                                                     tAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGc                                                          >  3:1117569/1‑151 (MQ=255)
                                                                                      aaTAGTACAAATTTTTATAGTAATTTCTCATGTACATATCTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAgg                                                           <  1:21209/149‑1 (MQ=255)
                                                                                        tAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCt                                                        >  2:358902/1‑150 (MQ=255)
                                                                                            aCAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGc                                                   >  3:96616/1‑151 (MQ=255)
                                                                                            aCAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGc                                                   <  3:60325/151‑1 (MQ=255)
                                                                                            aCAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGc                                                   >  3:569335/1‑151 (MQ=255)
                                                                                              aaaTTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAACCGTTGCCATACTCAAAAGGCCTAAGGcc                                                  >  3:239996/1‑150 (MQ=255)
                                                                                              aaaTTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCa                                                 <  2:890308/151‑1 (MQ=255)
                                                                                               aaTTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAg                                                <  2:706240/151‑1 (MQ=255)
                                                                                                   tttATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAggcg                                             <  2:115773/150‑1 (MQ=255)
                                                                                                   tttATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAggcg                                             <  3:1204429/150‑1 (MQ=255)
                                                                                                   tttATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGAATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAggcgg                                            >  3:647856/1‑151 (MQ=255)
                                                                                                    ttATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAggcggc                                           >  1:53407/1‑151 (MQ=255)
                                                                                                     tataGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGTCAGgcggcg                                          >  2:1099869/1‑151 (MQ=255)
                                                                                                     tataGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAggcggc                                           <  2:962359/150‑1 (MQ=255)
                                                                                                      ataGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGgcggcg                                          <  3:728127/150‑1 (MQ=255)
                                                                                                                 tcATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTAttt                               >  2:269918/1‑150 (MQ=255)
                                                                                                                  cATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTAtt                                <  3:793688/148‑1 (MQ=255)
                                                                                                                   aTGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTaa                             >  3:1169586/1‑150 (MQ=255)
                                                                                                                   aTGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTaa                             >  2:1361748/1‑150 (MQ=255)
                                                                                                                       aCATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACaa                         >  2:540560/1‑150 (MQ=255)
                                                                                                                       aCATATTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAAt                        <  2:1395329/151‑1 (MQ=255)
                                                                                                                         atatTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAAtt                       >  4:351741/1‑150 (MQ=255)
                                                                                                                          tatTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTAc                     >  2:1055756/1‑151 (MQ=255)
                                                                                                                           atTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTAc                     <  3:1383114/150‑1 (MQ=255)
                                                                                                                           atTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTAc                     <  3:23099/150‑1 (MQ=255)
                                                                                                                           atTTTTTTACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACg                    >  3:883842/1‑151 (MQ=255)
                                                                                                                             ttttttACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGc                   <  3:948970/150‑1 (MQ=255)
                                                                                                                               ttttACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCtt                >  3:336178/1‑151 (MQ=255)
                                                                                                                                  tACAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTc               >  1:56665/1‑149 (MQ=255)
                                                                                                                                    cAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCg           <  3:119976/151‑1 (MQ=255)
                                                                                                                                    cAGGATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCg           <  2:259072/151‑1 (MQ=255)
                                                                                                                                       gATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGa         >  2:223087/1‑150 (MQ=255)
                                                                                                                                       gATAAATAACAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGa         <  3:277417/150‑1 (MQ=255)
                                                                                                                                        ataaataaCAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAAt       <  2:1283800/151‑1 (MQ=255)
                                                                                                                                          aaataaCAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAAt       >  3:47255/1‑149 (MQ=255)
                                                                                                                                           aataaCAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGt    >  2:727916/1‑151 (MQ=255)
                                                                                                                                            ataaCAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTg   >  2:233491/1‑151 (MQ=255)
                                                                                                                                            ataaCAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTg   >  3:1375173/1‑151 (MQ=255)
                                                                                                                                             taaCAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTgg  >  2:59976/1‑151 (MQ=255)
                                                                                                                                             taaCAGGCAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTgg  >  2:1175807/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
GTTCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGCGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTGG  >  NC_000913/4337961‑4338253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: