Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 701,504 Δ1 bp coding (91/1221 nt) nagC ← N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913701,5020C.99.1% 521.1 / ‑4.0 109coding (93/1221 nt)nagCN‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base . (55/53);  total (55/54)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

GACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGAT  >  NC_000913/701355‑701647
                                                                                                                                                   |                                                                                                                                                 
gACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGccgt                                                                                                                                                <  2:859263/151‑3 (MQ=255)
gACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGccg                                                                                                                                                 <  1:378852/150‑2 (MQ=255)
 aCGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGccgta                                                                                                                                               <  3:947508/151‑4 (MQ=255)
 aCGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGccgta                                                                                                                                               <  3:756408/151‑4 (MQ=255)
 aCGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGccgta                                                                                                                                               <  4:2258/151‑4 (MQ=255)
 aCGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGccgta                                                                                                                                               <  2:535368/151‑4 (MQ=255)
  cGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGccgtac                                                                                                                                              >  2:1398662/1‑147 (MQ=255)
   gATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACt                                                                                                                                            >  2:126282/1‑151 (MQ=255)
   gATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACt                                                                                                                                            >  2:1132157/1‑151 (MQ=255)
      ggAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTgg                                                                                                                                          <  3:487148/150‑1 (MQ=255)
      ggAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGt                                                                                                                                         <  2:1002841/151‑1 (MQ=255)
            aGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATc                                                                                                                                    <  4:464225/150‑1 (MQ=255)
             gcgcGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAg                                                                                                                                  <  4:119064/151‑1 (MQ=255)
              cgcgGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAgg                                                                                                                                 >  3:1394225/1‑151 (MQ=255)
              cgcgGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAgg                                                                                                                                 <  2:556070/151‑1 (MQ=255)
               gcggcgGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAgg                                                                                                                                 >  3:1016748/1‑150 (MQ=255)
                cggcggCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCg                                                                                                                               <  1:228370/151‑1 (MQ=255)
                    ggcCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATaa                                                                                                                           <  4:475254/151‑1 (MQ=255)
                       ccccGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAAcc                                                                                                                        <  1:176728/151‑1 (MQ=255)
                          cGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAAccgcc                                                                                                                     <  3:106964/151‑1 (MQ=255)
                           ggTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAAccgccg                                                                                                                    >  3:866977/1‑151 (MQ=255)
                            gTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACcgccgc                                                                                                                   >  3:226751/1‑151 (MQ=255)
                            gTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACcgccgc                                                                                                                   >  1:303350/1‑151 (MQ=255)
                               gAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCt                                                                                                                <  2:372039/151‑1 (MQ=255)
                                aGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTg                                                                                                               >  3:1313382/1‑151 (MQ=255)
                                 gtccTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTg                                                                                                               <  4:344639/148‑1 (MQ=255)
                                 ggCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTg                                                                                                               <  3:502923/150‑1 (MQ=255)
                                 ggCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTg                                                                                                               <  3:334350/150‑1 (MQ=255)
                                 ggCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGt                                                                                                              >  3:1202917/1‑151 (MQ=255)
                                 ggCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGt                                                                                                              >  3:1396655/1‑151 (MQ=255)
                                    ctgctgATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGTCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTa                                                                                                            >  3:564026/1‑150 (MQ=255)
                                     tgctgATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTa                                                                                                            >  3:347976/1‑149 (MQ=255)
                                      gctgATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAg                                                                                                          <  1:212541/150‑1 (MQ=255)
                                      gctgATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAg                                                                                                          <  2:563995/150‑1 (MQ=255)
                                        tgATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTCCTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGc                                                                                                         <  3:94369/149‑1 (MQ=255)
                                         gATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGt                                                                                                      <  1:477341/151‑1 (MQ=255)
                                         gATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGt                                                                                                      >  1:48621/1‑151 (MQ=255)
                                             aaCTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGtttt                                                                                                   >  3:1122696/1‑150 (MQ=255)
                                             aaCTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGtttt                                                                                                   >  4:215515/1‑150 (MQ=255)
                                             aaCTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGtttt                                                                                                   <  3:842485/150‑1 (MQ=255)
                                                  cttTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAAGCGATAAAccgcc                                                                                                                     >  4:414853/1‑127 (MQ=255)
                                                  cttTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAAGCGATAAAccgcc                                                                                                                     <  1:414857/127‑1 (MQ=255)
                                                      gATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCaa                                                                                         <  2:387438/151‑1 (MQ=255)
                                                      gATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCa                                                                                          >  2:1108865/1‑150 (MQ=255)
                                                      gATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCa                                                                                          <  3:71348/150‑1 (MQ=255)
                                                          aGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACAtt                                                                                     >  4:245952/1‑151 (MQ=255)
                                                             ccGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTAcc                                                                                  >  3:856296/1‑151 (MQ=255)
                                                             ccGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTAcc                                                                                  >  2:1129462/1‑151 (MQ=255)
                                                              cgcgTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTAcc                                                                                  >  2:951452/1‑150 (MQ=255)
                                                               gcgTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCt                                                                                 <  3:1132209/150‑1 (MQ=255)
                                                                 gTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATc                                                                              >  3:534227/1‑151 (MQ=255)
                                                                    cGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGa                                                                           <  4:234287/151‑1 (MQ=255)
                                                                     gATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCt                                                                             >  3:137494/1‑148 (MQ=255)
                                                                     gATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGa                                                                           <  3:1162305/150‑1 (MQ=255)
                                                                            tGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCAAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGt                                                                     >  2:403138/1‑149 (MQ=255)
                                                                               cgcgTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGcc                                                                >  2:947491/1‑151 (MQ=255)
                                                                                gcgTAATTTTGGTTACGTTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAACCCGCGTGATTGGCCGTGCTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCt                                                               <  4:247186/151‑1 (MQ=255)
                                                                                gcgTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCt                                                               >  4:13675/1‑151 (MQ=255)
                                                                                gcgTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCt                                                               <  2:1202858/151‑1 (MQ=255)
                                                                                         tGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCAt                                                       <  2:1313312/150‑1 (MQ=255)
                                                                                         tGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCAt                                                       >  4:380398/1‑150 (MQ=255)
                                                                                          ggTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATa                                                      <  2:975681/150‑1 (MQ=255)
                                                                                          ggTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATAc                                                     <  1:195418/151‑1 (MQ=255)
                                                                                           gTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACt                                                    >  1:165744/1‑151 (MQ=255)
                                                                                              aCGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACttt                                                  >  3:678052/1‑150 (MQ=255)
                                                                                               cGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTtct                                                <  3:921401/151‑1 (MQ=255)
                                                                                                gCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTtctc                                               >  3:499688/1‑151 (MQ=255)
                                                                                                gCTGGCGGGCGTCGGGTGGCGTTACTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTtct                                                >  3:753347/1‑150 (MQ=255)
                                                                                                 cTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCcgcg                                                                                                                  <  4:240479/83‑1 (MQ=255)
                                                                                                 cTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCcgcg                                                                                                                  >  1:240482/1‑83 (MQ=255)
                                                                                                 cTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTctct                                              >  4:353345/1‑151 (MQ=255)
                                                                                                  tGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTtctc                                               <  2:866940/149‑1 (MQ=255)
                                                                                                      gggggCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGGGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCGTATTg                                         <  3:1377325/151‑1 (MQ=255)
                                                                                                        gggCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTg                                         >  3:312040/1‑149 (MQ=255)
                                                                                                          gCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGtt                                     >  2:851834/1‑151 (MQ=255)
                                                                                                            aaGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTa                                    <  2:80721/150‑1 (MQ=255)
                                                                                                            aaGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTAc                                   >  2:936908/1‑151 (MQ=255)
                                                                                                                tGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGAcc                               >  3:261361/1‑151 (MQ=255)
                                                                                                                tGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGAcc                               >  3:656568/1‑151 (MQ=255)
                                                                                                                 ggCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCt                              <  3:676974/151‑1 (MQ=255)
                                                                                                                  gCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTc                             >  3:207839/1‑151 (MQ=255)
                                                                                                                   cTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCg                            >  2:476376/1‑151 (MQ=255)
                                                                                                                    tttGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTc                             <  2:347947/149‑1 (MQ=255)
                                                                                                                     ttGCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGtt                          >  2:465833/1‑151 (MQ=255)
                                                                                                                       gCTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTAc                        <  1:445545/151‑1 (MQ=255)
                                                                                                                        cTCGGCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTAcc                       >  3:940763/1‑151 (MQ=255)
                                                                                                                           ggCAATCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGt                     <  3:600354/150‑1 (MQ=255)
                                                                                                                              aaTCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAAc                 <  3:1282976/151‑1 (MQ=255)
                                                                                                                              aaTCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAAc                 >  3:63294/1‑151 (MQ=255)
                                                                                                                                tCTGAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACg                >  1:173291/1‑150 (MQ=255)
                                                                                                                                   gAATCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACg                <  2:753314/147‑1 (MQ=255)
                                                                                                                                    aaTCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGAt              <  2:148526/148‑1 (MQ=255)
                                                                                                                                     aTCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTc          >  2:65486/1‑151 (MQ=255)
                                                                                                                                      tCCGCGAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCt         >  3:457047/1‑151 (MQ=255)
                                                                                                                                        cgcgAGATTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTg       <  2:1394154/151‑1 (MQ=255)
                                                                                                                                           gagaTTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTg    <  4:201514/151‑1 (MQ=255)
                                                                                                                                           gagaTTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTg    <  3:685682/151‑1 (MQ=255)
                                                                                                                                           gagaTTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTg    >  3:137646/1‑151 (MQ=255)
                                                                                                                                           gagaTTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTg    <  2:900249/151‑1 (MQ=255)
                                                                                                                                             gaTTGGCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGAt  >  3:1230447/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGAT  >  NC_000913/701355‑701647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: