Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 890,338 (T)7→6 coding (250/378 nt) ybjM → inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913890,3320T.99.1% 514.1 / ‑1.6 107coding (244/378 nt)ybjMinner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base . (63/43);  total (63/44)
Fisher's exact test for biased strand distribution p-value = 4.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

Only 100 of 112 total aligned reads displayed.

CGGGCTGCCGGGCATCCTGAAATCGGCTTGCTATTTTTCATTCTTCCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAGTTTGTTTAAACCACAGCACAGAAAAAATCAGTAAAGCCCTCAACGC  >  NC_000913/890185‑890478
                                                                                                                                                   |                                                                                                                                                  
cGGGCTGCCGGGCATCCTGAAATCGGCTTGCTATTTTTCATTCTTCCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTGtttt                                                                                                                                                 <  4:205316/151‑1 (MQ=255)
 gggCTGCCGGGCATCCTGAAATCGGCTTGCTATTTTTCATTCTTCCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTGttttt                                                                                                                                                >  1:148456/1‑151 (MQ=255)
 gggCTGCCGGGCATCCTGAAATCGGCTTGCTATTTTTCATTCTTCCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTGttttt                                                                                                                                                >  2:1382178/1‑151 (MQ=255)
      gCCGGGCATCCTGAAATCGGCTTGCTATTTTTCATTCTTCCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTGttttttca                                                                                                                                             <  4:251287/149‑3 (MQ=255)
      gCCGGGCATCCTGAAATCGGCTTGCTATTTTTCATTCTTCCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTGttttttca                                                                                                                                             <  3:1044717/149‑3 (MQ=255)
       ccGGGCATCCTGAAATCGGCTTGCTATTTTTCATTCTTCCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCg                                                                                                                                         >  3:1074954/1‑151 (MQ=255)
       ccGGGCATCCTGAAATCGGCTTGCTATTTTTCATTCTTCCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCg                                                                                                                                         >  3:1144639/1‑151 (MQ=255)
       ccGGGCATCCTGAAATCGGCTTGCTATTTTTCATTCTTCCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCg                                                                                                                                         <  2:261122/151‑1 (MQ=255)
       ccGGGCATCCTGAAATCGGCTTGCTATTTTTCATTCTTCCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCg                                                                                                                                         <  2:1028443/151‑1 (MQ=255)
        cGGGCATCCTGAAATCGGCTTGCTATTTTTCATTCTTCCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCg                                                                                                                                         >  2:126505/1‑150 (MQ=255)
         gggCATCCTGAAATCGGCTTGCTATTTTTCATTCTTCCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGAc                                                                                                                                       <  1:473810/151‑1 (MQ=255)
           gCATCCTGAAATCGGCTTGCTATTTTTCATTCTTCCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGAcgc                                                                                                                                     >  1:158016/1‑151 (MQ=255)
             aTCCTGAAATCGGCTTGCTATTTTTCATTCTTCCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACgcgc                                                                                                                                   >  3:390794/1‑151 (MQ=255)
             aTCCTGAAATCGGCTTGCTATTTTTCATTCTTCCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACgcgc                                                                                                                                   >  3:459135/1‑151 (MQ=255)
                cTGAAATCGGCTTGCTATTTTTCATTCTTCCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTc                                                                                                                                 >  3:578908/1‑150 (MQ=255)
                  gAAATCGGCTTGCTATTTTTCATTCTTCCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCAtt                                                                                                                              <  3:458152/151‑1 (MQ=255)
                  gAAATCGGCTTGCTATTTTTCATTCTTCCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCAtt                                                                                                                              >  4:170599/1‑151 (MQ=255)
                     aTCGGCTTGCTATTTTTCATTCTTCCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCt                                                                                                                            >  3:1299055/1‑150 (MQ=255)
                     aTCGGCTTGCTATTTTTCATTCTTCCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTg                                                                                                                           <  3:1349553/151‑1 (MQ=255)
                         gCTTGCTATTTTTCATTCTTCCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCaa                                                                                                                       <  2:91878/151‑1 (MQ=255)
                          cTTGCTATTTTTCATTCTTCCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCa                                                                                                                        >  3:782506/1‑149 (MQ=255)
                                aTTTTTCATTCTTCCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTgg                                                                                                                <  3:859298/151‑1 (MQ=255)
                                    ttCATTCTTCCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATg                                                                                                            >  1:396898/1‑151 (MQ=255)
                                     tCATTCTTCCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATg                                                                                                            <  2:485575/150‑1 (MQ=255)
                                     tCATTCTTCCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATg                                                                                                            <  2:579591/150‑1 (MQ=255)
                                     tCATTCTTCCTGGAGCAGTCGCCAGCTTCTTGTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCGCCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCa                                                                                                              <  3:738429/148‑1 (MQ=255)
                                       aTTCTTCCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGtt                                                                                                         <  2:220549/151‑1 (MQ=255)
                                       aTTCTTCCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGtt                                                                                                         >  3:839279/1‑151 (MQ=255)
                                         tcttcCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTa                                                                                                        >  2:950027/1‑150 (MQ=255)
                                           ttcCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACt                                                                                                      >  3:1294922/1‑150 (MQ=255)
                                           ttcCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACt                                                                                                      >  2:766127/1‑150 (MQ=255)
                                            tcCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTa                                                                                                        <  4:231386/148‑1 (MQ=255)
                                               tGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAgcg                                                                                                 >  2:1324059/1‑151 (MQ=255)
                                               tGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAgcg                                                                                                 >  3:74969/1‑151 (MQ=255)
                                               tGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAgcg                                                                                                 >  2:1241753/1‑151 (MQ=255)
                                                ggAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAgcgc                                                                                                <  2:1144583/151‑1 (MQ=255)
                                                ggAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAgcg                                                                                                 <  2:499318/150‑1 (MQ=255)
                                                 gAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGcgcg                                                                                               >  3:615180/1‑151 (MQ=255)
                                                  aGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCgg                                                                                              >  1:24908/1‑151 (MQ=255)
                                                  aGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCgg                                                                                              >  2:1061804/1‑151 (MQ=255)
                                                   gCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGgt                                                                                             >  1:279862/1‑151 (MQ=255)
                                                     aGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGgtgt                                                                                           >  3:703179/1‑151 (MQ=255)
                                                        cGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCt                                                                                        <  3:730122/151‑1 (MQ=255)
                                                         gCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTg                                                                                       <  2:864308/151‑1 (MQ=255)
                                                           cAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTgg                                                                                      >  3:251382/1‑150 (MQ=255)
                                                           cAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGgt                                                                                     >  2:936718/1‑151 (MQ=255)
                                                           cAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGAGTTCTGgt                                                                                     >  3:1158370/1‑151 (MQ=255)
                                                            aGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGgtg                                                                                    <  3:402665/151‑1 (MQ=255)
                                                               ttcttTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTgc                                                                                 >  2:597978/1‑151 (MQ=255)
                                                               ttcttTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTgc                                                                                 >  3:552587/1‑151 (MQ=255)
                                                                 cttTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTgcgc                                                                               >  1:304296/1‑151 (MQ=255)
                                                                   tttCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTgcg                                                                                <  3:950740/148‑1 (MQ=255)
                                                                   tttCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTg                                                                             >  2:239825/1‑151 (MQ=255)
                                                                    ttCACAGCGTAGAGAATTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCATTCTGTTCGATGCTCATTATGCTGCTG‑TTTTTTCCCTGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGAGGTGTTCTGGTGTGCGCt                                                                              <  3:545226/149‑1 (MQ=255)
                                                                      cacaGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTgggg                                                                          <  3:102148/151‑1 (MQ=255)
                                                                      cacaGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTggg                                                                           <  2:74959/150‑1 (MQ=255)
                                                                       acaGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTggggg                                                                         >  2:1304318/1‑151 (MQ=255)
                                                                         aGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCa                                                                       <  1:101585/151‑1 (MQ=255)
                                                                            gTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACtg                                                                    <  2:663039/151‑1 (MQ=255)
                                                                             tAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACtgt                                                                   >  3:1068890/1‑151 (MQ=255)
                                                                             tAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACtgt                                                                   >  1:176144/1‑151 (MQ=255)
                                                                              agagAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACtgtg                                                                  >  3:461132/1‑151 (MQ=255)
                                                                              agagAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACtgt                                                                   <  3:1036932/150‑1 (MQ=255)
                                                                              agagAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACtgt                                                                   <  3:592284/150‑1 (MQ=255)
                                                                                agaAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGtt                                                                >  2:153116/1‑151 (MQ=255)
                                                                                  aaGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGttt                                                               <  3:926676/150‑1 (MQ=255)
                                                                                  aaGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTc                                                              <  4:192833/151‑1 (MQ=255)
                                                                                  aaGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTc                                                              <  2:552556/151‑1 (MQ=255)
                                                                                   aGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCt                                                             <  3:1174204/151‑1 (MQ=255)
                                                                                             ccTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATc                                                     >  4:152708/1‑149 (MQ=255)
                                                                                              ctctGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCagt                                                  >  1:166556/1‑151 (MQ=255)
                                                                                               tctGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCagt                                                  >  3:1329797/1‑150 (MQ=255)
                                                                                                  gTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAgtt                                              >  2:127027/1‑151 (MQ=255)
                                                                                                     tGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAgtttgt                                           >  2:417231/1‑151 (MQ=255)
                                                                                                     tGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAgtttg                                            <  4:231956/150‑1 (MQ=255)
                                                                                                        cgcAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAgtttgttt                                         >  2:717363/1‑150 (MQ=255)
                                                                                                           aaTGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAGTTTGTTTAAAc                                     >  2:1063417/1‑151 (MQ=255)
                                                                                                           aaTGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAGTTTGTTTAAAc                                     <  2:636601/151‑1 (MQ=255)
                                                                                                                 ggcggcACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAGTTTGTTTAAACcaca                                 <  2:390758/149‑1 (MQ=255)
                                                                                                                  gcggcACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAGTTTGTTTAAACcacagc                               >  2:1070320/1‑150 (MQ=255)
                                                                                                                     gcACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAGTTTGTTTAAACcacagcac                             >  4:193111/1‑149 (MQ=255)
                                                                                                                      cACCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGGATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAGTTTGTTTAAACcccagcacata                          <  4:212510/151‑3 (MQ=255)
                                                                                                                       aCCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAGTTTGTTTAAACCACAGCACAGa                          >  3:741800/1‑150 (MQ=255)
                                                                                                                       aCCCTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAGTTTGTTTAAACCACAGCACAGa                          >  2:758929/1‑150 (MQ=255)
                                                                                                                         ccTGTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAGTTTGTTTAAACCACAGCACAGaaa                        >  3:143230/1‑150 (MQ=255)
                                                                                                                            gTTCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAGTTTGTTTAAACCACAGCACAGAAAAAAt                    >  4:108650/1‑151 (MQ=255)
                                                                                                                             ttCGATGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAGTTTGTTTAAACCACAGCACAGAAAAAATc                   >  3:1167267/1‑151 (MQ=255)
                                                                                                                                  tGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAGTTTGTTTAAACCACAGCACAGAAAAAATCAGTa               >  2:704450/1‑150 (MQ=255)
                                                                                                                                  tGCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAGTTTGTTTAAACCACAGCACAGAAAAAACCAGTa               <  2:578877/150‑1 (MQ=255)
                                                                                                                                   gCTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATCTATCAGTAGTTTGTTTAAACCACAGCACAGAAAAAATCAGTaa              >  3:488015/1‑150 (MQ=255)
                                                                                                                                    cTCATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAGTTTGTTTAAACCACAGCACAGAAAAAATCAGTaa              >  3:719181/1‑149 (MQ=255)
                                                                                                                                      cATTATGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAGTTTGTTTAAACCACAGCACAGAAAAAATCAGTAAAGcc          >  1:104935/1‑151 (MQ=255)
                                                                                                                                          aTGCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAGTTTGTTTAAACCACAGCACAGAAAAAATCAGTAAAGCCCTCa      <  1:433544/151‑1 (MQ=255)
                                                                                                                                            gCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAGTTTGTTTAAACCACAGCACAGAAAAAATCAGTAAAGCCCTCaa     <  3:133104/150‑1 (MQ=255)
                                                                                                                                            gCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAGTTTGTTTAAACCACAGCACAGAAAAAATCAGTAAAGCCCTCaa     <  3:782296/150‑1 (MQ=255)
                                                                                                                                            gCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAGTTTGTTTAAACCACAGCACAGAAAAAATCAGTAAAGCCCTCaa     <  2:37506/150‑1 (MQ=255)
                                                                                                                                            gCGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAGTTTGTTTAAACCACAGCACAGAAAAAATCAGTAAAGCCCTCAAc    <  2:1294854/151‑1 (MQ=255)
                                                                                                                                             cGGCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAGTTTGTTTAAACCACAGCACAGAAAAAATCAGTAAAGCCCTCAAcg   <  3:127043/151‑1 (MQ=255)
                                                                                                                                              ggCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAGTTTGTTTAAACCACAGCACAGAAAAAATCAGTAAAGCCCTCAAcgc  <  2:839240/151‑1 (MQ=255)
                                                                                                                                              ggCTG‑TTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAGTTTGTTTAAACCACAGCACAGAAAAAATCAATAAAGCCCTCAAcgc  >  1:381606/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGGGCTGCCGGGCATCCTGAAATCGGCTTGCTATTTTTCATTCTTCCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAGTTTGTTTAAACCACAGCACAGAAAAAATCAGTAAAGCCCTCAACGC  >  NC_000913/890185‑890478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑