Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 346,248 (A)7→8 coding (90/672 nt) yahN ← amino acid exporter for proline, lysine, glutamate, homoserine

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913346,2411.A98.3% 481.7 / ‑2.3 120coding (97/672 nt)yahNamino acid exporter for proline, lysine, glutamate, homoserine
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base A (54/64);  total (55/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

CACACTGCGTAATTAGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTAT  >  NC_000913/346091‑346386
                                                                                                                                                       |                                                                                                                                                 
cacaCTGCGTAATTAGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGAtt                                                                                                                                                    >  3:81440/1‑151 (MQ=255)
  cacTGCGTAATTAGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTa                                                                                                                                                  <  2:785463/150‑1 (MQ=255)
   acTGCGTAATTAGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTaa                                                                                                                                                 >  3:396939/1‑150 (MQ=255)
       cGTAATTAGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTaaaaaa                                                                                                                                             >  3:694170/1‑150 (MQ=255)
        gTAATTAGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTaaaaaa                                                                                                                                             >  2:196425/1‑149 (MQ=255)
         tAATTAGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTaaaaaaa                                                                                                                                            >  3:813673/1‑149 (MQ=255)
          aaTTAGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTaaaaaaaagt                                                                                                                                         <  2:74518/151‑4 (MQ=255)
          aaTTAGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTaaaaaaaagt                                                                                                                                         <  3:334569/151‑4 (MQ=255)
          aaTTAGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTaaaaaaaag                                                                                                                                          <  3:375133/150‑3 (MQ=255)
            ttAGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTaaaaaaaagta                                                                                                                                        <  2:1384974/150‑5 (MQ=255)
                cGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCa                                                                                                                                     <  4:242359/150‑1 (MQ=255)
                cGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCAc                                                                                                                                    <  1:198123/151‑1 (MQ=255)
                    gCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGaa                                                                                                                                 <  1:12392/150‑1 (MQ=255)
                      aaGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAAc                                                                                                                                >  3:571091/1‑149 (MQ=255)
                      aaGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAAc                                                                                                                                >  2:540359/1‑149 (MQ=255)
                       aGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGt                                                                                                                             >  1:403570/1‑151 (MQ=255)
                       aGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGt                                                                                                                             >  3:1369154/1‑151 (MQ=255)
                       aGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGt                                                                                                                             >  3:129416/1‑151 (MQ=255)
                        gACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTc                                                                                                                            >  2:580002/1‑151 (MQ=255)
                          ccAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGt                                                                                                                             >  4:51110/1‑148 (MQ=255)
                          ccAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTcca                                                                                                                          <  3:1242354/151‑2 (MQ=255)
                           caaacaaaCCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCt                                                                                                                          >  2:451348/1‑150 (MQ=255)
                             aacaaacaCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACg                                                                                                                       >  3:17719/1‑151 (MQ=255)
                             aacaaacCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACg                                                                                                                       <  3:1052847/151‑1 (MQ=255)
                                aaacCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTc                                                                                                                    >  3:950918/1‑151 (MQ=255)
                                 aacccaacccGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTc                                                                                                                    >  4:142993/1‑150 (MQ=255)
                                 aacccaacccGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCAGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTc                                                                                                                    >  1:120136/1‑150 (MQ=255)
                                  acccaacccGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAg                                                                                                                  <  2:396907/151‑1 (MQ=255)
                                  acccaacccGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAg                                                                                                                  <  2:864677/151‑1 (MQ=255)
                                    ccaacccGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGt                                                                                                                >  2:239485/1‑151 (MQ=255)
                                     caacccGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGt                                                                                                                >  1:362315/1‑150 (MQ=255)
                                     caacccGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTa                                                                                                               <  2:1111271/151‑1 (MQ=255)
                                        cccGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAAc                                                                                                            <  3:626650/151‑1 (MQ=255)
                                        cccGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAAc                                                                                                            >  4:269202/1‑151 (MQ=255)
                                         ccGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACg                                                                                                           <  2:1022233/151‑1 (MQ=255)
                                             aaTAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCAt                                                                                                       >  3:1098880/1‑151 (MQ=255)
                                              aTAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATg                                                                                                      <  3:220078/151‑1 (MQ=255)
                                              aTAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATg                                                                                                      <  3:980522/151‑1 (MQ=255)
                                                aaaaTGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGc                                                                                                     <  3:206114/150‑1 (MQ=255)
                                                aaaaTGCAGCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCa                                                                                                    <  3:815576/151‑1 (MQ=255)
                                                  aaTGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCaaa                                                                                                  <  4:253056/151‑1 (MQ=255)
                                                  aaTGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCaaa                                                                                                  <  1:428822/151‑1 (MQ=255)
                                                   aTGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAg                                                                                                 <  3:573994/151‑1 (MQ=255)
                                                      cATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGAt                                                                                              >  2:932045/1‑151 (MQ=255)
                                                          gCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCAt                                                                                          >  3:84833/1‑151 (MQ=255)
                                                           cccAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCAt                                                                                          >  3:427245/1‑150 (MQ=255)
                                                           cccAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCAt                                                                                          >  2:426857/1‑150 (MQ=255)
                                                           cccAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATa                                                                                         <  2:438828/151‑1 (MQ=255)
                                                            ccAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAg                                                                                        <  1:66347/151‑1 (MQ=255)
                                                              aGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTg                                                                                      >  3:730313/1‑151 (MQ=255)
                                                                  ccACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGAttt                                                                                  <  3:330921/151‑1 (MQ=255)
                                                                    aCGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCa                                                                                <  1:211749/151‑1 (MQ=255)
                                                                      gCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATc                                                                              >  4:166818/1‑151 (MQ=255)
                                                                         cAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCat                                                                           <  1:224213/151‑1 (MQ=255)
                                                                          aGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCata                                                                          <  3:1151787/151‑1 (MQ=255)
                                                                           gCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACTACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCata                                                                          >  2:1253874/1‑150 (MQ=255)
                                                                           gCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCataa                                                                         <  2:1405680/151‑1 (MQ=255)
                                                                           gCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCataa                                                                         <  3:1264700/151‑1 (MQ=255)
                                                                           gCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCataa                                                                         <  1:359448/151‑1 (MQ=255)
                                                                            cccGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCataaa                                                                        <  3:540393/151‑1 (MQ=255)
                                                                             ccGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCataaat                                                                       <  3:608366/151‑1 (MQ=255)
                                                                                   aGCGCCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACCGTCAGGTAAACGGCATGCAAAGGATCCATAGTGAATTCATCCATAAATAAGTGa                                                                 <  4:186395/151‑1 (MQ=255)
                                                                                    gCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGa                                                                 >  2:1120080/1‑150 (MQ=255)
                                                                                     cACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGaac                                                               >  2:1077516/1‑151 (MQ=255)
                                                                                     cACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGaac                                                               <  2:27325/151‑1 (MQ=255)
                                                                                       ccccTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGaacta                                                             <  2:813639/151‑1 (MQ=255)
                                                                                       ccccTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGaact                                                              >  1:126428/1‑150 (MQ=255)
                                                                                        cccTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGaacta                                                             >  2:160090/1‑150 (MQ=255)
                                                                                           tGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTg                                                         >  2:878563/1‑151 (MQ=255)
                                                                                             cgcgTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGc                                                        >  3:1047349/1‑150 (MQ=255)
                                                                                               cgTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGcatc                                                     <  2:571061/151‑1 (MQ=255)
                                                                                                  cGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGcatcat                                                   >  3:975091/1‑150 (MQ=255)
                                                                                                  cGACCGGAAGCCAGGCTGGGTTATACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGc                                                        <  3:401829/145‑1 (MQ=255)
                                                                                                   gACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCatat                                                 <  3:135721/151‑1 (MQ=255)
                                                                                                   gACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCatat                                                 >  2:286773/1‑151 (MQ=255)
                                                                                                      cGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTc                                              <  3:1291880/151‑1 (MQ=255)
                                                                                                       ggAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCt                                             >  2:1168483/1‑151 (MQ=255)
                                                                                                        gAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCt                                             >  2:1092350/1‑150 (MQ=255)
                                                                                                            ccAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCaa                                        <  3:887341/151‑1 (MQ=255)
                                                                                                            ccAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCa                                         >  3:352844/1‑150 (MQ=255)
                                                                                                                gCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAaat                                    <  2:394247/151‑1 (MQ=255)
                                                                                                                   ggTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAaataa                                  <  3:451382/150‑1 (MQ=255)
                                                                                                                    gTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAaata                                   <  1:471635/148‑1 (MQ=255)
                                                                                                                    gTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATc                                >  2:929471/1‑151 (MQ=255)
                                                                                                                        gTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGt                            >  2:1318714/1‑151 (MQ=255)
                                                                                                                            tacCACAAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCg                         >  3:920335/1‑150 (MQ=255)
                                                                                                                               cacaAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgag                       >  3:68834/1‑149 (MQ=255)
                                                                                                                                acaAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgagaga                    <  2:81428/151‑1 (MQ=255)
                                                                                                                                acaAAGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgagaga                    <  3:426887/151‑1 (MQ=255)
                                                                                                                                  aaaGAGATTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgagagaga                  <  4:232887/151‑1 (MQ=255)
                                                                                                                                       gaTTGGCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATcaca             <  2:950881/151‑1 (MQ=255)
                                                                                                                                         ttGGCTCCCGAATAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACaa            <  2:1224783/149‑1 (MQ=255)
                                                                                                                                          tGGCTCCCGGATTAAAAAAAATAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGAGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGCGCGAGAGAGATCACAACgt         >  3:160195/1‑151 (MQ=255)
                                                                                                                                           ggCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAgt         >  2:1298282/1‑151 (MQ=255)
                                                                                                                                           ggCTCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAg          <  2:694145/150‑1 (MQ=255)
                                                                                                                                              tCCCGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTc      >  2:427846/1‑151 (MQ=255)
                                                                                                                                               cccGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTc      <  3:1106707/150‑1 (MQ=255)
                                                                                                                                               cccGGATTAAAAAAAAGTAATCACGAACAGGCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAGCTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGGGAGATCACAAAGTGTCt     <  3:1127409/151‑1 (MQ=255)
                                                                                                                                                 cGGATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCtt    >  2:976159/1‑150 (MQ=255)
                                                                                                                                                  ggATTAAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTAt  <  2:84821/151‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                 
CACACTGCGTAATTAGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTAT  >  NC_000913/346091‑346386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: