Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 484,337 (C)6→7 coding (67/3150 nt) acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913484,3311.C99.0% 358.8 / ‑6.0 100L25V (CTG→GTG) acrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  major base C (44/55);  minor base A (1/0);  total (45/55)
Fisher's exact test for biased strand distribution p-value = 4.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 115 total aligned reads displayed.

GTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGTTATT  >  NC_000913/484182‑484476
                                                                                                                                                      |                                                                                                                                                 
gTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGc                                                                                                                                                  <  3:103982/151‑1 (MQ=255)
gTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGc                                                                                                                                                  >  3:1349192/1‑151 (MQ=255)
  tATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGccc                                                                                                                                                >  2:495289/1‑151 (MQ=255)
   aTCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGcccc                                                                                                                                               <  3:1282691/151‑1 (MQ=255)
   aTCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGcccc                                                                                                                                               >  1:468560/1‑151 (MQ=255)
    tCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGccccc                                                                                                                                              >  3:176766/1‑151 (MQ=255)
     cGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGcccccc                                                                                                                                             >  2:787735/1‑151 (MQ=255)
     cGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGcccccc                                                                                                                                             >  1:43212/1‑151 (MQ=255)
     cGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGccccc                                                                                                                                              <  1:140056/150‑1 (MQ=255)
      gATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGccccccc                                                                                                                                            <  3:283445/151‑2 (MQ=255)
       aTACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGccccccct                                                                                                                                           <  4:359/151‑3 (MQ=255)
       aTACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGccccccc                                                                                                                                            >  4:399281/1‑149 (MQ=255)
         aCCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGccccccctgc                                                                                                                                         <  1:470394/151‑5 (MQ=255)
           cGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGccccccctgcc                                                                                                                                        >  3:457928/1‑145 (MQ=255)
              tCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAAc                                                                                                                                      >  4:389687/1‑150 (MQ=255)
               cATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACat                                                                                                                                    >  1:87837/1‑151 (MQ=255)
               cATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACat                                                                                                                                    <  3:1111013/151‑1 (MQ=255)
                atatTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACatg                                                                                                                                   >  2:65125/1‑151 (MQ=255)
                      tGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATgatgat                                                                                                                              >  1:161420/1‑150 (MQ=255)
                      tGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATgatgat                                                                                                                              >  3:59497/1‑150 (MQ=255)
                        ttCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAAt                                                                                                                           >  2:1187569/1‑151 (MQ=255)
                          cGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATgg                                                                                                                         >  2:307786/1‑151 (MQ=255)
                           gATAACCTGTGTCACCGTGTCCTTCACTGTTTTCGCATCAGCGCCGGCGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGc                                                                                                                        <  1:412616/151‑1 (MQ=255)
                               aCCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGACCCCCCTGCCAACATGATGATAATGGCGATc                                                                                                                    >  4:250828/1‑151 (MQ=255)
                                ccTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATc                                                                                                                    >  1:444464/1‑150 (MQ=255)
                                  tgtgTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCAcc                                                                                                                 >  2:1296423/1‑151 (MQ=255)
                                   gtgtCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCAccc                                                                                                                <  1:393706/151‑1 (MQ=255)
                                   gtgtCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCAc                                                                                                                  <  4:10769/149‑1 (MQ=255)
                                     gtCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCa                                                                                                               <  3:1322031/150‑1 (MQ=255)
                                        aCCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGc                                                                                                            >  3:105842/1‑150 (MQ=255)
                                         ccGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCa                                                                                                           >  3:188812/1‑150 (MQ=255)
                                           gtgtCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCaaa                                                                                                         >  3:298931/1‑150 (MQ=255)
                                              tCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAAt                                                                                                      <  3:787769/150‑1 (MQ=255)
                                                  gCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGc                                                                                                 <  2:325775/151‑1 (MQ=255)
                                                   cACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGcg                                                                                                >  3:1122931/1‑151 (MQ=255)
                                                   cACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGcg                                                                                                >  2:1277360/1‑151 (MQ=255)
                                                       gTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGcgatcg                                                                                            <  4:336978/151‑1 (MQ=255)
                                                       gTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGcgatc                                                                                             <  3:807232/150‑1 (MQ=255)
                                                          ttCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGcgatcgat                                                                                          <  3:704121/150‑1 (MQ=255)
                                                           tCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATa                                                                                         <  3:566202/150‑1 (MQ=255)
                                                            cGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATaaa                                                                                       <  3:420704/151‑1 (MQ=255)
                                                            cGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATaa                                                                                        >  3:505321/1‑150 (MQ=255)
                                                                  aGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAAtt                                                                                 >  3:655866/1‑151 (MQ=255)
                                                                    cgcCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAg                                                                               <  3:674994/151‑1 (MQ=255)
                                                                     gcCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAgg                                                                              >  3:523457/1‑151 (MQ=255)
                                                                     gcCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAgg                                                                              <  2:826359/151‑1 (MQ=255)
                                                                      ccGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAgg                                                                              >  3:874832/1‑150 (MQ=255)
                                                                        ggggTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCa                                                                            >  3:1119863/1‑150 (MQ=255)
                                                                        ggggTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCAt                                                                           >  4:136497/1‑151 (MQ=255)
                                                                         gggTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATg                                                                          <  1:249201/151‑1 (MQ=255)
                                                                             aggaggCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCt                                                                       <  3:994981/150‑1 (MQ=255)
                                                                             aggaggCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCt                                                                       <  4:134311/150‑1 (MQ=255)
                                                                              ggaggCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCtt                                                                      >  3:920153/1‑150 (MQ=255)
                                                                              ggaggCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTa                                                                     <  3:346985/151‑1 (MQ=255)
                                                                              ggaggCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTa                                                                     <  2:1222775/151‑1 (MQ=255)
                                                                               gaggCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTaa                                                                    <  4:23175/151‑1 (MQ=255)
                                                                               gaggCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTaa                                                                    <  2:874797/151‑1 (MQ=255)
                                                                                aggCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAAc                                                                   <  2:1185423/151‑1 (MQ=255)
                                                                                 ggCGGAGATCGTTACTGCCGGCTGTGCAATCGTAGGATATTGCGCCGCCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAAc                                                                   <  1:18120/150‑1 (MQ=255)
                                                                                 ggCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACg                                                                  >  1:102655/1‑151 (MQ=255)
                                                                                  gCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACgg                                                                 <  2:298903/151‑1 (MQ=255)
                                                                                  gCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACgg                                                                 <  1:79818/151‑1 (MQ=255)
                                                                                   cGGAGATCGTTACTGCCGGCGGTGCAAACGTAGGAAATTGCGCCACCGGCAGTTTGAGGAATGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACgg                                                                 >  1:236613/1‑150 (MQ=255)
                                                                                    ggAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCCCGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAAAgg                                                                 >  2:942311/1‑149 (MQ=255)
                                                                                      agaTCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTcc                                                             >  3:748883/1‑151 (MQ=255)
                                                                                           gTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGttt                                                        <  1:22120/151‑1 (MQ=255)
                                                                                           gTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGttt                                                        <  2:162395/151‑1 (MQ=255)
                                                                                                 gCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTttaagtta                                                  <  2:105826/151‑1 (MQ=255)
                                                                                                       ggTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACtt                                            <  2:59486/151‑1 (MQ=255)
                                                                                                          gCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTgg                                          <  4:444460/150‑1 (MQ=255)
                                                                                                              tCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTg                                      >  2:1225446/1‑150 (MQ=255)
                                                                                                               cGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGtt                                    >  1:71887/1‑151 (MQ=255)
                                                                                                               cGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGtt                                    <  2:1072270/151‑1 (MQ=255)
                                                                                                                  aGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTc                                   >  2:491208/1‑149 (MQ=255)
                                                                                                                  aGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCa                                  <  3:1296492/150‑1 (MQ=255)
                                                                                                                  aGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAg                                 <  2:801162/151‑1 (MQ=255)
                                                                                                                   ggATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTc                                   <  4:236610/148‑1 (MQ=255)
                                                                                                                   ggATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAgg                                <  3:1299695/151‑1 (MQ=255)
                                                                                                                     atatTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCt                              <  1:306007/151‑1 (MQ=255)
                                                                                                                       atTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTg                             >  2:729902/1‑150 (MQ=255)
                                                                                                                         tGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGc                          >  3:502773/1‑151 (MQ=255)
                                                                                                                          gcgcCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGc                          >  3:513754/1‑150 (MQ=255)
                                                                                                                          gcgcCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCa                         <  3:1296587/151‑1 (MQ=255)
                                                                                                                                ccGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCtt                   >  3:847367/1‑151 (MQ=255)
                                                                                                                                ccGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCtt                   <  3:307815/151‑1 (MQ=255)
                                                                                                                                 cGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTg                  >  4:343255/1‑151 (MQ=255)
                                                                                                                                   gCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCg                <  2:849445/151‑1 (MQ=255)
                                                                                                                                   gCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCg                <  3:1386729/151‑1 (MQ=255)
                                                                                                                                    cAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCgg               <  2:188785/151‑1 (MQ=255)
                                                                                                                                        ttGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCtt            <  3:1277426/150‑1 (MQ=255)
                                                                                                                                        ttGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCtt            <  2:176740/150‑1 (MQ=255)
                                                                                                                                         tGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGc          >  4:209192/1‑151 (MQ=255)
                                                                                                                                           aGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTg        >  1:166125/1‑151 (MQ=255)
                                                                                                                                           aGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTg        >  3:858939/1‑151 (MQ=255)
                                                                                                                                             gATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTgg       <  3:298809/150‑1 (MQ=255)
                                                                                                                                              aTCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGtt     <  2:505286/151‑1 (MQ=255)
                                                                                                                                                cGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGttat   <  3:1187626/151‑1 (MQ=255)
                                                                                                                                                cGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGttat   <  1:170957/151‑1 (MQ=255)
                                                                                                                                                cGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGtta    <  4:237612/150‑1 (MQ=255)
                                                                                                                                                 gCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGttatt  >  1:245235/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                 
GTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGTTATT  >  NC_000913/484182‑484476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: