BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,734,534	+C	coding (501/732 nt)	yfiH ←	UPF0124 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,734,532	1	.	C	89.7%	410.1 / 38.2	126	coding (503/732 nt)	yfiH	UPF0124 family protein
Reads supporting (aligned to +/- strand): ref base . (5/8); new base C (55/58); total (60/66)
Fisher's exact test for biased strand distribution p-value = 5.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 126 total aligned reads displayed.

AACGGTCGCCGCCGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAA‑CCTCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGA  >  NC_000913/2734389‑2734676

aaCGGTCGCCGCCGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAA‑CCccccc                                                                                                                                           <  3:556295‑M1/151‑6 (MQ=255)

aaCGGTCGCCGCCGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAA‑CCccccc                                                                                                                                           >  3:697140‑M1/1‑146 (MQ=255)

 aCGGTCGCCGCCGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAA‑CCcccccc                                                                                                                                          <  1:465221‑M1/151‑7 (MQ=255)

   ggTCGCCGCCGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAA‑CCccccccca                                                                                                                                        <  1:188878‑M1/151‑9 (MQ=255)

        ccgccgAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcg                                                                                                                                    <  2:488108‑M1/151‑13 (MQ=255)

        ccgccgAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttc                                                                                                                                     <  2:1011306‑M1/150‑12 (MQ=255)

         cgccgAAAATTTGCTCAACCCCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAA‑CC‑CCCCCCCCACTTc                                                                                                                                  <  3:181207/150‑1 (MQ=255)

           ccgAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaac                                                                                                                                 <  3:1208633‑M1/151‑16 (MQ=255)

           ccgAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaac                                                                                                                                 <  3:422053‑M1/151‑16 (MQ=255)

            cgAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacg                                                                                                                                <  3:1367360‑M1/151‑17 (MQ=255)

                aaTTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcg                                                                                                                            <  3:879823‑M1/151‑21 (MQ=255)

                    tGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtgg                                                                                                                         >  2:442441‑M1/1‑127 (MQ=255)

                     gCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAA‑CC‑CCCCCCCCACTTCGAACGCGCGTGGa                                                                                                                     <  3:238229/151‑1 (MQ=255)

                       tCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggacc                                                                                                                      >  2:479943‑M1/1‑124 (MQ=255)

                        cAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggacca                                                                                                                     >  4:71301‑M1/1‑123 (MQ=255)

                        cAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgc                                                                                                                               <  4:157078‑M1/140‑18 (MQ=255)

                           cacCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattg                                                                                                                 <  1:437645‑M1/151‑32 (MQ=255)

                           cacCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattg                                                                                                                 <  2:727659‑M1/151‑32 (MQ=255)

                            ccccaCGTTCGCCAGACTCTGCCGGGTAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTACTTTAGCGCCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattg                                                                                                                 <  3:814770‑M1/149‑32 (MQ=255)

                                aCGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggc                                                                                                            >  2:965311‑M1/1‑115 (MQ=255)

                                 cGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggcc                                                                                                           <  4:447885‑M1/151‑38 (MQ=255)

                                  gTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccc                                                                                                          >  1:309246‑M1/1‑113 (MQ=255)

                                    tCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccct                                                                                                         <  2:1385250‑M1/150‑40 (MQ=255)

                                    tCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccc                                                                                                          >  4:438906‑M1/1‑111 (MQ=255)

                                     cGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggcccta                                                                                                        <  3:94411‑M1/150‑41 (MQ=255)

                                       ccAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaac                                                                                                      <  3:34472‑M1/150‑43 (MQ=255)

                                              cTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcg                                                                                                >  2:1225141‑M1/1‑101 (MQ=255)

                                              cTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcg                                                                                                >  2:836582‑M1/1‑101 (MQ=255)

                                               tGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcg                                                                                                >  3:159531‑M1/1‑100 (MQ=255)

                                                gCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCGCCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCcccccctcatcgaacgcgcgtggaccaattgccggccctaaccaggcgaga                                                                                             <  2:816930‑M1/150‑52 (MQ=255)

                                                gCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAA‑CCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaa                                                                                            <  2:900825‑M1/150‑53 (MQ=255)

                                                gCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAA‑CCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgaga                                                                                             <  2:330514‑M1/149‑52 (MQ=255)

                                                 ccGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaat                                                                                           >  3:1032857‑M1/1‑98 (MQ=255)

                                                 ccGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaat                                                                                           <  2:1276607‑M1/151‑54 (MQ=255)

                                                  cGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaata                                                                                          <  4:253756‑M1/151‑55 (MQ=255)

                                                  cGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaat                                                                                           >  3:82577‑M1/1‑97 (MQ=255)

                                                    ggCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatatt                                                                                        <  2:706665‑M1/151‑57 (MQ=255)

                                                    ggCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccactacgaacgcgcgtggaccaattgccggccctaaccaggcgagaatatt                                                                                        <  3:816190‑M1/151‑57 (MQ=255)

                                                     gCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattt                                                                                       >  2:339374‑M1/1‑94 (MQ=255)

                                                      cAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatatttt                                                                                      >  2:185031‑M1/1‑93 (MQ=255)

                                                       aaGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttc                                                                                     <  2:1169704‑M1/151‑60 (MQ=255)

                                                        aGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttcc                                                                                    >  2:396995‑M1/1‑91 (MQ=255)

                                                        aGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttcc                                                                                    <  2:254844‑M1/151‑61 (MQ=255)

                                                           tGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccgg                                                                                  >  1:13510‑M1/1‑88 (MQ=255)

                                                            gATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggat                                                                                <  3:197652‑M1/151‑65 (MQ=255)

                                                            gATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggat                                                                                <  3:79736‑M1/151‑65 (MQ=255)

                                                            gATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccgg                                                                                  <  2:1113370‑M1/149‑63 (MQ=255)

                                                             aTAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCCCTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccgga                                                                                 <  3:73509‑M1/149‑64 (MQ=255)

                                                              tAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggatt                                                                               >  3:1097782‑M1/1‑85 (MQ=255)

                                                               aaaTATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattat                                                                             >  2:143197‑M1/1‑84 (MQ=255)

                                                                aaTACCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGACTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatc                                                                            >  3:13871‑M1/1‑83 (MQ=255)

                                                                   atCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagc                                                                         >  4:281676‑M1/1‑80 (MQ=255)

                                                                   atCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagc                                                                         >  1:27275‑M1/1‑80 (MQ=255)

                                                                   atCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcag                                                                          <  2:1279309‑M1/150‑71 (MQ=255)

                                                                     ccGCCAGATACTTATCACCATGCTAGATCAAAGCTGCACTAGCTTCAGCGTCTCCTGCCTTAAACGCGTCGC‑G‑A‑CCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTATGCAGGCGTGAATATTTTCCGGATTATCAGCa                                                                     <  3:41670/151‑1 (MQ=255)

                                                                      cGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTTCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaa                                                                      <  3:292645‑M1/151‑75 (MQ=255)

                                                                         cAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaaca                                                                   <  1:137788‑M1/151‑78 (MQ=255)

                                                                         cAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaaca                                                                   <  1:103515‑M1/151‑78 (MQ=255)

                                                                          aGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaac                                                                    >  2:970323‑M1/1‑73 (MQ=255)

                                                                            aTACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacagga                                                                <  1:281196‑M1/151‑81 (MQ=255)

                                                                             tACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacagga                                                                <  3:736512‑M1/150‑81 (MQ=255)

                                                                             tACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacagga                                                                >  4:55285‑M1/1‑70 (MQ=255)

                                                                              aCTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaa                                                               <  3:1034412‑M1/150‑82 (MQ=255)

                                                                              aCTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaa                                                               <  3:1264199‑M1/150‑82 (MQ=255)

                                                                                ttATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaacc                                                            >  4:399317‑M1/1‑67 (MQ=255)

                                                                                 tATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaac                                                             <  3:481580‑M1/149‑84 (MQ=255)

                                                                                 tATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaac                                                             <  4:440565‑M1/149‑84 (MQ=255)

                                                                                  aTCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaaccg                                                           <  2:917516‑M1/150‑86 (MQ=255)

                                                                                    cACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaaccgtct                                                        >  2:1359390‑M1/1‑63 (MQ=255)

                                                                                     aCCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAA‑CC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGtc                                                      >  2:1108907/1‑150 (MQ=255)

                                                                                      ccATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaaccgtctct                                                      <  1:191863‑M1/151‑91 (MQ=255)

                                                                                       cATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaaccgtctctt                                                     >  1:159197‑M1/1‑60 (MQ=255)

                                                                                        aTGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaaccgtctcttc                                                    >  1:391193‑M1/1‑59 (MQ=255)

                                                                                         tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaaccgtctcttcc                                                   >  3:763620‑M1/1‑58 (MQ=255)

                                                                                          gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaaccgtctcttcca                                                  <  2:606062‑M1/151‑95 (MQ=255)

                                                                                           cTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaaccgtctcttcc                                                   >  3:95247‑M1/1‑56 (MQ=255)

                                                                                             gaatgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaaccgtctcttccagca                                               <  2:686080‑M1/151‑98 (MQ=255)

                                                                                                 gaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaaccgtctcttccagcacgcc                                           >  1:75381‑M1/1‑50 (MQ=255)

                                                                                                   aaGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaaccgtctcttccagcacgcctg                                         <  3:143213‑M1/151‑104 (MQ=255)

                                                                                                    aGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaaccgtctcttccagcacgcctgc                                        >  2:1324867‑M1/1‑47 (MQ=255)

                                                                                                       tGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaaccgtctcttccagcacgcctgcgc                                      >  2:798668‑M1/1‑44 (MQ=255)

                                                                                                         cACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaaccgtctcttccagcacgcctgcgcaca                                   <  1:154216‑M1/151‑110 (MQ=255)

                                                                                                         cACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaaccgtctcttccagcacgcctgcgcac                                    >  2:523272‑M1/1‑42 (MQ=255)

                                                                                                               cTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaaccgtctcttccagcacgcctgcgcacagtccac                             >  2:596020‑M1/1‑36 (MQ=255)

                                                                                                                tttAGTGTCTACTGCCATAAACGCCTCGCGGAC‑CCccccccacttcgaacccgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggtaaccgtctcttccagtacgcctgcgcacagtccacg                            >  4:465869‑M1/1‑35 (MQ=255)

                                                                                                                   aGCGTCTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaaccgtctcttccagcacgcctgcgcacagtccacgcc                          <  3:1252590‑M1/150‑119 (MQ=255)

                                                                                                                      gTCTACTGCCATAAACCCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaaccgtctcttccagcacgcccgcgcacagtccacgccagc                       >  3:955727‑M1/1‑29 (MQ=255)

                                                                                                                        cTACTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaaccgtctcttccagcacgcctgcgcacagtccacgccagcc                      >  2:530410‑M1/1‑27 (MQ=255)

                                                                                                                          aCTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaaccgtctcttccagcacgcctgcgcacagtccacgccagcca                     <  3:442474‑M1/148‑124 (MQ=255)

                                                                                                                          aCTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaaccgtctcttccagcacgcctgcgcacagtccacgccagcca                     <  1:23093‑M1/148‑124 (MQ=255)

                                                                                                                           cTGCCATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaaccgtctcttccagcacgcctgcgcacagtccacgccagccagcat                 >  1:297160‑M1/1‑24 (MQ=255)

                                                                                                                               cATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaaccgtctcttccagcacgcctgcgcacagtccacgccagccagcatgagc             <  2:609914‑M1/151‑132 (MQ=255)

                                                                                                                               cATAAACGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaaccgtctcttccagcacgcctgcgcacagtccacgccagccagcatgagc             >  3:1115885‑M1/1‑20 (MQ=255)

                                                                                                                                   aaCGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaaccgtctcttccagcacgcctgcgcacagtccacgccagccagcatgagcggcg         >  4:260699‑M1/1‑16 (MQ=255)

                                                                                                                                   aaCGCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaaccgtctcttccagcacgcctgcgcacagtccacgccagccagcatgagcggcg         <  2:95233‑M1/151‑136 (MQ=255)

                                                                                                                                      gCCTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaaccgtctcttccagcacgcctgcgcacagtccacgccagccagcatgagcggcggc       >  3:1008697‑M1/1‑13 (MQ=255)

                                                                                                                                        cTCGC‑GAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaaccgtctcttccagcacgcctgcgcacagtccacgccagccagcatgagcggcggcgac    >  3:1191205‑M1/1‑11 (MQ=255)

                                                                                                                                          cgc‑gAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaaccgtctcttccagcacgcctgcgcacagtccacgccagccagcatgagcggcggcgactt  <  3:965348‑M1/151‑143 (MQ=255)

                                                                                                                                          cgc‑gAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaaccgtctcttccagcacgcctgcgcacagtccacgccagccagcatgagcggcggcgac    <  2:15928‑M1/149‑141 (MQ=255)

                                                                                                                                           gc‑gAACCCccccccacttcgaacgcgcgtggaccaattgccggccctaaccaggcgagaatattttccggattatcagcaaaacaggaaaacgtctcttccagcacggctgcgcacagtccacgccagccagcatgagcggcggcgactt  >  2:1029913‑M1/1‑8 (MQ=255)

AACGGTCGCCGCCGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAA‑CCTCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGA  >  NC_000913/2734389‑2734676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`