Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,665,7020CA43.6% 161.3 / 62.0 110A37S (GCG→TCG) gadXacid resistance regulon transcriptional activator; autoactivator
Reads supporting (aligned to +/- strand):  ref base C (49/13);  new base A (0/48);  total (49/61)
Fisher's exact test for biased strand distribution p-value = 1.86e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

AGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATT  >  NC_000913/3665552‑3665848
                                                                                                                                                         |                                                                                                                                                  
aGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGc                                                                                                                                                    <  1:328085/151‑1 (MQ=255)
  tgatgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCa                                                                                                                                                   >  2:504574/1‑150 (MQ=255)
        aCGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCa                                                                                                                                            <  1:257698/151‑1 (MQ=255)
        aCGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTCAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCa                                                                                                                                            <  2:875253/151‑1 (MQ=255)
              aaTGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCa                                                                                                                                       <  1:37187/150‑1 (MQ=255)
              aaTGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCa                                                                                                                                       <  1:392157/150‑1 (MQ=255)
               aTGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCAcc                                                                                                                                     >  1:409302/1‑151 (MQ=255)
                 gaagaaACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCg                                                                                                                                    <  3:586052/150‑1 (MQ=255)
                  aagaaACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGcc                                                                                                                                  >  3:424194/1‑151 (MQ=255)
                       aCTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTa                                                                                                                              >  2:248712/1‑150 (MQ=255)
                          tttttATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAAt                                                                                                                          >  3:1161764/1‑151 (MQ=255)
                             ttATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGc                                                                                                                       >  2:907377/1‑151 (MQ=255)
                             ttATGTGCATGAAGATTTAATCCCTCCTCCTTGAGCATCGGAAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑ACCACACTTATCTACTCAATTTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAg                                                                                                                        <  4:416679/150‑1 (MQ=255)
                              ccaatctaggAAGTTTTAATCCCTCCTCTTTGAGCATCGGGAAAAATAATAAAAGCGTCCCCCTTGACACAAATAAAAAATTTAC‑AACACCCTTACCTACACAAATTGGG‑C‑TGGCACCCCAAAAAACCAGGTCACCGCCATTAAAATAGc                                                                                                                       <  4:167150/141‑1 (MQ=255)
                               aTGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCg                                                                                                                      >  2:931786/1‑150 (MQ=255)
                                  tgCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGatat                                                                                                                  >  3:53940/1‑151 (MQ=255)
                                     aTGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGc                                                                                                                       >  1:167152/1‑143 (MQ=255)
                                        aaGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCAcc                                                                                                             >  3:862913/1‑150 (MQ=255)
                                        aaGATTTAATGCCTCCTCCTTGAGCACCGGGAGAAATAATAAAAGCGTGCCCCTTGACACAAATAAAAAATTTTC‑AACACATTTATCTACTCGATTTTGG‑C‑TGGCATCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTc                                                                                                                <  1:451350/147‑1 (MQ=255)
                                        aaGATTTAATCCCCCCTCCTTGAGCATGGGAAGAAATAAGAAAAGCGTGCCCCTGGACAGAAATAAAAAATTTTC‑AACACATTTATCTACTCAATTTTGT‑C‑TTCCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCAc                                                                                                              <  2:70289/149‑1 (MQ=255)
                                          gATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCAtt                                                                                                          >  2:30935/1‑151 (MQ=255)
                                            tttAATGCCTCCTCCTTGAGCATCGGGAAAAATAATAAAAGCGTGTCCTTTGACACAAATAAAAAATTTTC‑AACACACTTATCTACTCAAATTTGG‑C‑TTGCATCCGAAAAACCCAGTTCACCGCCATTAAAATAGCGATATTCACCAtt                                                                                                          <  1:280213/149‑1 (MQ=255)
                                            tttAATCCCCCCCCCTGGAGCAGCGGGAAAAATAATGAAAGCGTGCCCTTTGACAAAAATAAAAAATTTTC‑AACACCCTTACCTACTCAATTTTGT‑T‑TTCCCCCCGAAAAACCCGGGTCACCGCCATTAAAATAGCGATATTCACCAt                                                                                                           <  3:142800/148‑1 (MQ=255)
                                               aaTGCCTCCTCCTTGAGCAGCGGAAAAAATAATAAAGGCGTGTCCCTTGACACAAATAAAAATTTTTC‑AACCCTCTTATCTACTAAATTTTGG‑T‑TTCCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                      <  3:704477/150‑1 (MQ=255)
                                               aaTCCCCCCTCCGTGACCATGGGAAAAAAAAATGAAGGCGTGCCCCTTGACACAAATAAAAAATTTTC‑AACACACTTATCTCCTCAAATTTGG‑C‑TTGCATCCCAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCa                                                                                                     <  2:917134/151‑1 (MQ=255)
                                                aTGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCAt                                                                                                    >  3:338898/1‑151 (MQ=255)
                                                   cctcctcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGt                                                                                                 >  3:465349/1‑151 (MQ=255)
                                                   cctcctcctTGAGCATCGGGAAAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATg                                                                                                   <  1:183966/149‑1 (MQ=255)
                                                   ccccctcttGGAGCATGGGGAAAAATAATGAAAGCTTGCCCCTTGACACAAAAAAAAATTTTTC‑AACACCTTTATCTACAAAATTTTGG‑T‑TTGCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATg                                                                                                   <  4:37527/146‑1 (MQ=255)
                                                    ctcctcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTg                                                                                                >  4:10412/1‑151 (MQ=255)
                                                    ctcctcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTg                                                                                                >  3:1012727/1‑151 (MQ=255)
                                                    cccccctttGAGCCTGGGGAAAAAAAATGAAGGCGTGCCCCTTGACACAAATA‑AAAATTTTCAAACACATTTATCTCCTCGATTTTGG‑C‑TTCCCCCCGAAAAAACCGGTCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTg                                                                                                <  2:424164/144‑1 (MQ=255)
                                                      cctcctTGAGCATCGGGAAAAATAATGAAAGCGTGTCCCTTGACACGAATAAAAATTTTTA‑AACACACTTATCTCCTCAATTTTGG‑C‑TTGCATCCGAAAAAACCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTg                                                                                                <  1:307993/149‑1 (MQ=255)
                                                      cccgtttggccgtCGGAAAAAAAAATAAAACCGTGCCCCGTGACAGAAAAACAAATTTTTC‑ACCACACTTCTCTACTGGATTTGGG‑C‑TTGCCTCCTAAAAACCCGGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTg                                                                                                <  3:368838/137‑1 (MQ=255)
                                                        tcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTgaga                                                                                             >  3:1151735/1‑150 (MQ=255)
                                                         ctttGAGAAGCGGGAAAAAAAATAAAGGCGTGCCCCTTAACACAAAAAAAAAATTTAA‑ACCACCCTTATCTCCTAG‑ATTTGG‑CTTTCCACCCAAAAAACCCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTgaga                                                                                             <  3:1221005/147‑1 (MQ=255)
                                                             gAGCATCGGGAAAAATAATGAAAGCGTGTCCCTTGACACAAATAAAAAATTTTC‑AACACACTTATCTACTCAATTTTGG‑C‑TTGCATCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAatata                                                                                        <  1:145226/150‑1 (MQ=255)
                                                                 accggaaaaaaaaaTAAAAGCGTGTCCCTTGACACAAATAAAAATTTTCC‑AACCCATTTTTCTACTCAATTTTGG‑T‑TTGCACCCCAAAAAACCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAttt                                                                                     <  4:280570/141‑1 (MQ=255)
                                                                    gggAAAAATAAAGAAGGCGTGCCCCTTGACACGAAAAAAAATTTTCC‑AACACCCTTATCTCCTCAATTTTGG‑T‑TTCCACCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTAt                                                                                   <  3:126823/148‑1 (MQ=255)
                                                                    gggAAAAAAAATGAAAGCGTGTCCTTTAAAACAAAAAAAAAATTTCA‑AACCCATTTATCTCCACAATTTTGG‑T‑TTCCACCCGAAAAAACCAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                      <  4:409298/145‑1 (MQ=255)
                                                                    ggaagaAATAATGAAGGCGTGTCCTTTGACACAAATAAAAAATTTTC‑AACACCCTTATCTACTCAATTTTGG‑C‑TTGCACCCAAAAAAACCAGGACACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGt                                                                                 <  3:285014/147‑1 (MQ=255)
                                                                     ggAAAAAAAAAGAAAGCGTTTCCCTTGCCCCAAAAAAAAAATTTTC‑AACCCCCTTTTTTACCCGAATTTGG‑T‑TTGCAGCCCAAAAACCCCAGTCACCGCCATTAAAATAGCGATATTCCCCATTAACCATGGTGAGAATATATTTATGTCt                                                                               <  1:60573/151‑1 (MQ=255)
                                                                     gaaaaaaTAATAAAAGCGTGTCCATGAACAAGAAAAAAAAATTTCC‑AACCCCCTTATCTACTAGATTTGGG‑C‑TTCCACCCCAAAAAACCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCt                                                                               <  3:172207/147‑1 (MQ=255)
                                                                      gagaAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCtt                                                                              >  2:445963/1‑151 (MQ=255)
                                                                       aaaaaaaaTAAAGGCGTGCCCTTTGACACAAAAAAAAATTTTTC‑AACCCATTTATCTACTAAATTTTGG‑T‑TTCCCCCCAAAAAAACAGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTg                                                                             <  4:460577/149‑1 (MQ=255)
                                                                       aaaaaaaaTAAAAGGGTTCCCCTTGACAAAAAAAAAAAATTTTA‑AACACACTTTTCTCCCCGAATTTGG‑C‑TTGCCCCCGAAAAAACCAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTg                                                                             <  2:614215/149‑1 (MQ=255)
                                                                       aaaaaaaaTAAAAGCGTTCCCCTTGACACAAAAAAAAAATTTCA‑AACCCACTTATCTACAAAATTTTGG‑T‑TTCCATCCGAAAAAACGAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCtt                                                                              <  4:152452/148‑1 (MQ=255)
                                                                         aaaTAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGc                                                                            >  2:151194/1‑150 (MQ=255)
                                                                         aaaTAATGAAAGCGTGTCCCTTGACACAAAAAAAAAATTTTC‑AACACTTTTATCTACTCAATTTTGG‑T‑TTGCACCCGAAAAAACGGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGc                                                                            <  3:360800/150‑1 (MQ=255)
                                                                         aaaTAATAAAAGCGTGCCCCTTAACACGAAAAAAAAATTTAC‑AACACCCTTATCTACTAAATTTTGG‑T‑TTCCCCCCAAAAAAACCAGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCa                                                                           <  3:884058/151‑1 (MQ=255)
                                                                            taatGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATAc                                                                        >  2:1085407/1‑151 (MQ=255)
                                                                             aatGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACg                                                                       >  2:1160105/1‑151 (MQ=255)
                                                                              atGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGc                                                                      >  1:139729/1‑151 (MQ=255)
                                                                                  aaGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAAtt                                                                  >  3:688901/1‑151 (MQ=255)
                                                                                    gCGTTTCCCTTCCCAAAAATCAAAAATTTTA‑AACACCCTTATTAACT‑GAATTTGGAT‑TTGCTCCCGCAAAATCCGGGACACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAAtt                                                                  <  1:68067/149‑1 (MQ=255)
                                                                                     cGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAAtt                                                                  >  3:518755/1‑148 (MQ=255)
                                                                                     cGTGTCCCTTGACACGAAAAAAAATTTTCC‑AACACTCTTTTCTACAAAATTTTGG‑C‑TTCCTCCCAAAAAAACCAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTa                                                                 <  4:427876/149‑1 (MQ=255)
                                                                                      gtgtCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                              >  4:306288/1‑151 (MQ=255)
                                                                                       tgtCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                              >  3:382082/1‑150 (MQ=255)
                                                                                          cctttGACACAAATAAAAATTTTTC‑AACACATTTATCTCCTCAAATTGGG‑T‑TTGCACCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTa                                                                 <  2:716591/141‑1 (MQ=255)
                                                                                           cctaaacacGAAAATAAAATTTTC‑AACACACTTAT‑TGCCCCAATTTTG‑CTTTACACCCAAAAAAGCAAGGATACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTc                                                         <  3:1356928/146‑1 (MQ=255)
                                                                                           ccTGGACACAAATAAAAATTTTTC‑AACACCCTTATCTACCCGATTTTGG‑T‑TTCCACCCGAAAAAACGAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTc                                                         <  1:474518/151‑1 (MQ=255)
                                                                                              tGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTccc                                                       >  2:671031/1‑150 (MQ=255)
                                                                                              tGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTccc                                                       >  3:328810/1‑150 (MQ=255)
                                                                                               gACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCAt                                                     >  2:919638/1‑151 (MQ=255)
                                                                                               gACACAAAAAAAAAATTTTC‑AACCCACTTATCTACTCAATTTTGG‑T‑TTGCACCCGAAAAAACCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCa                                                      <  3:581746/150‑1 (MQ=255)
                                                                                                acacAAATAAAAAATTTTC‑AACACACTTATCTACTCAATTTGGG‑C‑TTCCACCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCAt                                                     <  3:248737/150‑1 (MQ=255)
                                                                                                acacAAAAAAAAAATTTTC‑AACACATTTATCTACTCAAATTTGC‑C‑TTCCACCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATg                                                    <  3:1290478/151‑1 (MQ=255)
                                                                                                acacAAAAAAAAAATTTAC‑AACACCTTTATTTACTAAATTTTGT‑C‑TTCCATCCAAAAAAACCAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCa                                                      <  3:1281284/149‑1 (MQ=255)
                                                                                                    gAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGt                                                >  3:899363/1‑151 (MQ=255)
                                                                                                      aTACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTg                                               >  4:303723/1‑150 (MQ=255)
                                                                                                      aTACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTg                                               >  2:397676/1‑150 (MQ=255)
                                                                                                          aaaaTTTTC‑AACACTCTTATCTACTCGATTTTGG‑C‑TTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTg                                           <  2:164132/150‑1 (MQ=255)
                                                                                                          aaaaTTTCC‑AACACTCTTATCTACTGAAATTTGG‑T‑TTGCATCCGCAAAAACCAGGACACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGc                                          <  3:565745/151‑1 (MQ=255)
                                                                                                              ttttC‑AACACACTTATCTACTCAAATTGGG‑C‑TGGCACCCAAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATa                                       <  2:619839/150‑1 (MQ=255)
                                                                                                              ttttA‑AACACTCTTATCTACTCGATTTTGG‑T‑TTGCATCCAAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCAt                                        <  2:53929/149‑1 (MQ=255)
                                                                                                                ttccacccctattttCTACTCAAATTTGG‑C‑TTCCACCCAAAAAACCCAGGTCGCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAg                                      <  3:1193825/136‑1 (MQ=255)
                                                                                                                ttC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                                     <  1:253249/150‑1 (MQ=255)
                                                                                                                    aaCACTCTTATCTCCACAATTTTGG‑C‑TTCCACCCGAAAAAACCAGTCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGtt                                    <  3:397124/148‑1 (MQ=255)
                                                                                                                    aaCACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGAc                                 >  3:983674/1‑151 (MQ=255)
                                                                                                                      cacacTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACtt                               >  2:609926/1‑151 (MQ=255)
                                                                                                                         acTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAat                            >  3:1285311/1‑151 (MQ=255)
                                                                                                                               cTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTAc                       >  3:71451/1‑150 (MQ=255)
                                                                                                                               cTACACGAATTTGG‑C‑TTGCACCAGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATAtt                         <  3:931821/148‑1 (MQ=255)
                                                                                                                                 aCTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                     >  2:21469/1‑150 (MQ=255)
                                                                                                                                 aCTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                     >  3:879532/1‑150 (MQ=255)
                                                                                                                                  cTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATa                    >  3:563161/1‑150 (MQ=255)
                                                                                                                                  cTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATa                    >  3:1033419/1‑150 (MQ=255)
                                                                                                                                  cTCGAATTTGG‑C‑TTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAata                           <  2:600123/143‑1 (MQ=255)
                                                                                                                                      attttGG‑C‑TTCCACCCGAAAAAACCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATaaa                  <  3:418379/146‑1 (MQ=255)
                                                                                                                                      aaTTTGG‑G‑TCCCATCCGAAAAAACCAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATaa                   <  1:165130/147‑1 (MQ=255)
                                                                                                                                      aaTTGGG‑C‑TGCCTTCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATaaa                  <  1:458578/148‑1 (MQ=255)
                                                                                                                                        tttGG‑C‑TGGCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACata              <  3:1407173/150‑1 (MQ=255)
                                                                                                                                         ttGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATAtt            >  2:215400/1‑151 (MQ=255)
                                                                                                                                          tttgttttGCATCCAAAAAACCGAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATaaa                  <  4:468180/140‑1 (MQ=255)
                                                                                                                                             ggttttcttCCACAAAACCCAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTa           <  2:1161708/142‑1 (MQ=255)
                                                                                                                                                ttGCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTg        <  2:338869/150‑1 (MQ=255)
                                                                                                                                                  gCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGttt     >  2:688339/1‑151 (MQ=255)
                                                                                                                                                    aTCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTttat   >  3:1278031/1‑151 (MQ=255)
                                                                                                                                                      ccGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTttatt  >  3:227150/1‑150 (MQ=255)
                                                                                                                                                         |                                                                                                                                                  
AGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑C‑TTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATT  >  NC_000913/3665552‑3665848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑