Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913213,3410AG47.5% 122.4 / 45.9 101G337G (GGA→GGGtilStRNA(Ile)‑lysidine synthetase
Reads supporting (aligned to +/- strand):  ref base A (4/49);  new base G (48/0);  total (52/49)
Fisher's exact test for biased strand distribution p-value = 1.87e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

CCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCG  >  NC_000913/213196‑213491
                                                                                                                                                 |                                                                                                                                                      
ccTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAg                                                                                                                                                      >  1:464779/1‑148 (MQ=255)
  tCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAggg                                                                                                                                                    >  3:635319/1‑146 (MQ=255)
   cACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAgtg                                                                                                                                                    >  1:308693/1‑147 (MQ=255)
   cACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACa                                                                                                                                                <  4:262658/151‑1 (MQ=255)
   cACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACa                                                                                                                                                <  3:844196/151‑1 (MQ=255)
     cccTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACa                                                                                                                                                >  2:1081683/1‑149 (MQ=255)
           ttACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGACAGCTTaa                                                                                                                                          >  2:1295553/1‑149 (MQ=255)
           ttACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTaa                                                                                                                                          >  1:5213/1‑149 (MQ=255)
           ttACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAt                                                                                                                                         <  3:107442/150‑1 (MQ=255)
            tACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAgagaataaattaatt                                                                                                                                        >  2:607217/1‑136 (MQ=255)
            tACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGGACAGCTTaa                                                                                                                                          >  2:135938/1‑148 (MQ=255)
              cGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCgg                                                                                                                                     <  3:795206/151‑1 (MQ=255)
               gTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGGGGGGAGTGTACAGCTTAAttccgg                                                                                                                                    >  4:365052/1‑146 (MQ=255)
                  tGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGGGGGGAGGGGACAGCTTTATGCGGGAgg                                                                                                                                 >  3:1207349/1‑151 (MQ=255)
                  tGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGGTTAAAACgggggg                                                                                                                                 >  2:82552/1‑151 (MQ=255)
                   gggCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAgg                                                                                                                                 <  2:261219/150‑1 (MQ=255)
                   gggCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGc                                                                                                                                <  4:452928/151‑1 (MQ=255)
                    ggCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCg                                                                                                                               <  3:1098761/151‑1 (MQ=255)
                      cgcgTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGGTTAATGCGGGAGGCg                                                                                                                               >  2:303541/1‑149 (MQ=255)
                      cgcgTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCTTTATGCgggggg                                                                                                                                 >  2:827769/1‑147 (MQ=255)
                       gcgTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGAAAGCTTAATGCgggggggg                                                                                                                               >  1:323446/1‑146 (MQ=255)
                            tGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTACAGCCTaaatagggggggggta                                                                                                                            >  3:1071542/1‑132 (MQ=255)
                              aaaTCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAAATCGGGAGGCGGTATTTGccc                                                                                                                     >  2:1126487/1‑151 (MQ=255)
                                aTCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTc                                                                                                                   <  3:265201/151‑1 (MQ=255)
                                aTCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTc                                                                                                                   <  1:441608/151‑1 (MQ=255)
                                  ccGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTcc                                                                                                                  <  3:997788/150‑1 (MQ=255)
                                  ccGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCcg                                                                                                                 <  4:446916/151‑1 (MQ=255)
                                   cGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCcgc                                                                                                                <  3:1172938/151‑1 (MQ=255)
                                    gACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCcgcg                                                                                                               <  3:655899/151‑1 (MQ=255)
                                    gACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCcgcg                                                                                                               <  4:293587/151‑1 (MQ=255)
                                     aCGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAATGCGGGGGGGGGTATTTGcccccc                                                                                                                  >  3:1223610/1‑147 (MQ=255)
                                      cGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGGGGCGATATTCGCCCTCCGCGTg                                                                                                             >  1:11337/1‑151 (MQ=255)
                                            cAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCCTAATGCGGGGGGCGGGATTCGcccccccc                                                                                                                >  3:306332/1‑140 (MQ=255)
                                            cAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGAc                                                                                                        <  3:1251249/150‑1 (MQ=255)
                                            cAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGAAGGCGATATTCGCCCTCCGCGTGCAGAc                                                                                                        <  3:607244/150‑1 (MQ=255)
                                             aGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGACAGCTTAATTCGGGAGGCGGTATTTGCCCTCCGCGTg                                                                                                             >  1:386075/1‑144 (MQ=255)
                                              gTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGaa                                                                                                     <  2:1151646/151‑1 (MQ=255)
                                                cGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGGACAACTTAAATCGGGGGGGGGTATTTGCCCCCCGCCTGCCGACGaa                                                                                                     >  3:593469/1‑149 (MQ=255)
                                                cGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTTATTCGGGGGGGGGTATTCGCCCTCCCCCTGGAGACg                                                                                                       >  3:1075456/1‑147 (MQ=255)
                                                cGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAATTCGGGAGGGGATATTTGCCCCCCGCGGGCAGACgga                                                                                                     >  4:126692/1‑147 (MQ=255)
                                                 gCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGTAAAGCTTAATGCGGGGGGGGGTATTCGCCCTCCGCGGGCAGACGaa                                                                                                     >  2:753468/1‑148 (MQ=255)
                                                 gCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAATGCGGGGGGGGATATTTGCCCCCCCCGGGCCGACGGAGCg                                                                                                  >  2:277517/1‑151 (MQ=255)
                                                  cAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGGACAGCTTAATGGGGGAGGGGATATTCGCCCTCCGCGGGGAGACGAAGCgg                                                                                                 >  1:197492/1‑151 (MQ=255)
                                                   aaCTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTAAAGCTTAATTAGGGGGGCGATATTTGCCCTCCGCGTGCAGACGAAGCggg                                                                                                >  3:413481/1‑150 (MQ=255)
                                                   aaCTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGAGTACAGCTTAATGCGGGGGGGGATATTTGCCCCCCGCGGGCAGACGAAGCgg                                                                                                 >  2:695364/1‑150 (MQ=255)
                                                       gtggtgGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCa                                                                                              <  3:1022494/149‑1 (MQ=255)
                                                       gtggtgGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCa                                                                                              <  4:382396/149‑1 (MQ=255)
                                                       gtggtgGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCa                                                                                              <  3:277544/149‑1 (MQ=255)
                                                            ggATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAgcgtgca                                                                                       <  3:1295621/151‑2 (MQ=255)
                                                             gATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGACAGCTTAATGAGGGAGGGGGGATTTGCCCTCCGCGTGCAGACGAAACGGTCAgcgtgcg                                                                                       >  2:1220866/1‑150 (MQ=255)
                                                              aTTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGTACAGCTTAAATCGGGGGGGGGTATTCGCCCCCCCCGGGCAGACGAAACGGTCAgcgggcggt                                                                                     >  4:471575/1‑149 (MQ=255)
                                                              aTTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGtt                                                                                     <  4:418268/151‑1 (MQ=255)
                                                              aTTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGtt                                                                                     <  3:863621/151‑1 (MQ=255)
                                                                tAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGttt                                                                                    <  4:481196/150‑1 (MQ=255)
                                                                 aaaTCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGCGGGGAACGTTAAGTTTTATCCGGGGGGGGAGAATTATCCACCCCGCGCTGACGAAACGGGCAgcgtg                                                                                         >  3:773370/1‑144 (MQ=255)
                                                                 aaaTCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTACAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTc                                                                                   <  3:1179261/150‑1 (MQ=255)
                                                                        tCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTGCAGCTTAATGCGGGGGGGGGGATTTGCCCCCCGCGGGCAGACGAAGAGGGCAGCGGGCGGTTTAAAGCGcc                                                                           >  3:746307/1‑151 (MQ=255)
                                                                         cACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCa                                                                          <  1:310767/151‑1 (MQ=255)
                                                                           ccGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAGCTTAATGCGGGGGGGGATATTCGCCCTCCGCGTGCAGACGAAGCGGGCAGCGGGCGGTTCAAAACGcc                                                                           >  2:1392029/1‑148 (MQ=255)
                                                                            cGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGa                                                                       <  2:582097/151‑1 (MQ=255)
                                                                            cGGACAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCTTAATGCGGGAGGCGGTATTTCCCCCCCCCGCGCCGAAGAAGCGGGCAGCGGGCGGTTCAAAACGcc                                                                           >  3:1273870/1‑147 (MQ=255)
                                                                              ggCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGAtt                                                                     <  4:60221/151‑1 (MQ=255)
                                                                              ggCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGAtt                                                                     <  2:491510/151‑1 (MQ=255)
                                                                               gCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATtg                                                                    <  2:577421/151‑1 (MQ=255)
                                                                               gCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCCGGGAAGGGTACAGCTTAATTGGGGGGGGGATATTCGCCCCCCGCGGGCAGAAGGAGCGGGCAGCGGGGGTTTCcaaaggcc                                                                           >  1:117171/1‑139 (MQ=255)
                                                                                 aaaGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATtgct                                                                  <  4:204364/151‑1 (MQ=255)
                                                                                    gCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCa                                                               <  3:4787/151‑1 (MQ=255)
                                                                                     cGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCat                                                              <  3:1126540/151‑1 (MQ=255)
                                                                                      gAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGGGCAGACGAAGCGGGCAGCGGGCGTTTCAAAGCGCCAGGATTTCTGCat                                                              >  2:1377638/1‑150 (MQ=255)
                                                                                      gAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCata                                                             <  2:327993/151‑1 (MQ=255)
                                                                                        aaaCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAGCTTAATGCGGGGGGCGATATTTGCCCTCCGCGTGCAGACGAAGCGGGCAGCGGGCGGTTCAAAACGCCCGGATTGCTGCATAtt                                                           >  2:537431/1‑151 (MQ=255)
                                                                                        aaaCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAACTTAATTCGGGGGGCGATATTCGCCCTCCCCGTGCAGACGAAGCGGGCAGCGTGCGGTTTAAAACGCCAGGGTTGCTGGATAtt                                                           >  2:1035604/1‑151 (MQ=255)
                                                                                        aaaCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATAtt                                                           <  4:318242/151‑1 (MQ=255)
                                                                                         aaCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTg                                                          <  2:306302/151‑1 (MQ=255)
                                                                                          aCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTTCCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCCGCGTGCGTTTCAAAGGGCGAGTATTGCTGCATATTGt                                                         <  3:1068492/151‑1 (MQ=255)
                                                                                            aTTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGAAAGCTTAATTCGGGGGGGGATATTTGCCCTCCCCGGGGAGACGAAAAGGGCCGCGGGGGGTTTAAAGCGCCCGGAGTGCTGCATAtttt                                                         >  4:322580/1‑147 (MQ=255)
                                                                                                 gCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCg                                                   <  3:842783/150‑1 (MQ=255)
                                                                                                 gCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGt                                                  <  2:934686/151‑1 (MQ=255)
                                                                                                  ccGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAATGCGGGAGGCGATATTTGCCCCCCGCGTGCAGACGAAGCGGGCAGCGGGCGGTTCAAAACGCCCGGATTGCTGCCTATTGTCGGGCg                                                   >  2:658958/1‑149 (MQ=255)
                                                                                                   cGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCTTAATGCGGGGGGCGAGATTTGCCCTCCCCGGGCAGAAGAAGCGGTCAGCGGGCGGTTCAAAACGCCAGGATTGCTGCATATTGTTggg                                                     >  3:276984/1‑146 (MQ=255)
                                                                                                    gTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGGACAGCTTAATGCGGGGGGGGGTATTCGCCCTCCGCGTGCAGACGAAGCGGGCAGCGGGCGGTTTAAAACGCCAGGATTGCTGCATATTTTCGGGCGGaa                                                >  2:1284885/1‑150 (MQ=255)
                                                                                                       gCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAAcggc                                            <  1:245251/151‑1 (MQ=255)
                                                                                                       gCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAAcggc                                            <  1:171220/151‑1 (MQ=255)
                                                                                                         aGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCCCCCCCGGCAGACGAAACGGGCAGCGGGGGTTTTAAAAGGCCAGGATTGCTGCATATTTTCGGGCGGAAcggcgg                                          >  4:68425/1‑151 (MQ=255)
                                                                                                             gTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGt                                      <  2:413450/151‑1 (MQ=255)
                                                                                                             gTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGt                                      <  3:763923/151‑1 (MQ=255)
                                                                                                                gCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAg                                   <  2:217620/151‑1 (MQ=255)
                                                                                                                 cTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAATTCGGGGGGCGATATTCTCCCTCCCCGCGCAGAAGAAACGGGCAGCGGGCGTTTCAAAACGCCAGGAATTCTTCATATTGTCGGGCGGAAaggcgg                                          >  4:340387/1‑143 (MQ=255)
                                                                                                                 cTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAACTTAAATCGGGGGGGGATATTCGCCCTCCCCGTGCAGACGAAGCGGGCCGCGTGCGTTTCAAAACGCCAGGAGTGCTGCATATTGTCGGGGGGAACGGCGGGCGGAAg                                   >  2:518539/1‑150 (MQ=255)
                                                                                                                  ttCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAGCTTAATGCGGGGGGGGATATTCCCCCCCCCCGTGCAGACGAAGCGGGCCGCGGGCGTTTTAAAACGCCCGGATTGCTGCATATTGTCGGGCGTAACGGCGGGCGGaa                                    >  3:1043176/1‑148 (MQ=255)
                                                                                                                  ttCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGGAAACCTTTAATCGGGGGGGGATATTTGCCCCCCCCCGGCAGACGAAGCGGGCAGCGCGCGGTTTAAAGCGCCCGGGTTTCTGCATATTTTGGGGGGGAACAGCGGGCggga                                    >  3:934490/1‑145 (MQ=255)
                                                                                                                    cAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTaa                               <  2:534773/151‑1 (MQ=255)
                                                                                                                     aaCCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTaaa                              <  2:635293/151‑1 (MQ=255)
                                                                                                                       ccGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGa                            <  3:827803/151‑1 (MQ=255)
                                                                                                                       ccGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGa                            <  1:466824/151‑1 (MQ=255)
                                                                                                                          cTGGAATTACCGGCGGGGGCGGGGAGTGTACAGCTTAATGCGGGGGGGGATATTTGCCCCCCCCGTGCCGAAGAAGCGGGCAGCGGGCGTTTTCAAGCGCCAGGATTGCTGCATATTTTTGGGCGTGACGGCGGAGGTAAgggaaa                              >  3:665937/1‑141 (MQ=255)
                                                                                                                                    cggcggGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCCCCGCGTGCAGGCGAAGCGGGGAGCGTGCGTTTCAAAGCGCCCGGATTGCTGCCTATTGTCGGGGGTAACGGCGGGCGAAAGCTAAAGAAAATCTTGCAag                >  3:498430/1‑150 (MQ=255)
                                                                                                                                      gcggGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTCAAGAAAATCTGGGaa                 >  3:391001/1‑147 (MQ=255)
                                                                                                                                                 aaGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTgccg  <  4:134654/151‑1 (MQ=255)
                                                                                                                                                 aaGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTgcc   >  3:1232836/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                      
CCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCG  >  NC_000913/213196‑213491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: