Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913599,5760TG45.8% 179.6 / 53.1 118V288G (GTG→GGG) cusAcopper/silver efflux system, membrane component
Reads supporting (aligned to +/- strand):  ref base T (6/58);  new base G (54/0);  total (60/58)
Fisher's exact test for biased strand distribution p-value = 3.16e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 128 total aligned reads displayed.

AAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAA  >  NC_000913/599426‑599723
                                                                                                                                                       |                                                                                                                                                    
aaaCGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGggt                                                                                                                                                      <  2:999059/151‑1 (MQ=255)
  aCGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGggtgg                                                                                                                                                    <  1:145755/151‑1 (MQ=255)
   cGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGgggggg                                                                                                                                                    >  2:185013/1‑150 (MQ=255)
   cGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGggtggt                                                                                                                                                   <  2:579259/151‑1 (MQ=255)
      tCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGggggggggg                                                                                                                                                 >  3:567012/1‑147 (MQ=255)
      tCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGgggggggg                                                                                                                                                  >  2:323000/1‑147 (MQ=255)
        gacgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑Ggggggggggggggacc                                                                                                                                              >  3:719871/1‑145 (MQ=255)
        gacgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATcc                                                                                                                                              <  3:1378400/151‑1 (MQ=255)
         acgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCt                                                                                                                                             <  2:32872/151‑1 (MQ=255)
           gacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGGGGTcc                                                                                                                                              >  2:1081702/1‑148 (MQ=255)
           gacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGgggggggggttctg                                                                                                                                            >  4:251347/1‑142 (MQ=255)
            acTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCTGc                                                                                                                                           >  2:970284/1‑150 (MQ=255)
            acTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCCGc                                                                                                                                           >  2:954836/1‑150 (MQ=255)
             cTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGgggggg                                                                                                                                                    >  2:793807/1‑140 (MQ=255)
             cTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTcc                                                                                                                                              >  2:709356/1‑146 (MQ=255)
              tttAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGGGGTcc                                                                                                                                              >  1:24582/1‑145 (MQ=255)
               ttAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGa                                                                                                                                         <  2:94424/149‑1 (MQ=255)
                tAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGATCCTGCGA‑Tcc                                                                                                                                     >  2:670171/1‑151 (MQ=255)
                 aaTCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCg                                                                                                                                    <  2:1168536/151‑1 (MQ=255)
                  aTCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCgg                                                                                                                                   <  3:1098820/151‑1 (MQ=255)
                  aTCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGTGAGCCTGCGA‑TACgg                                                                                                                                   >  4:285141/1‑151 (MQ=255)
                     acaTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCaa                                                                                                                                <  1:121724/151‑1 (MQ=255)
                         cGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGGTGTCCTGCGG‑TCCGGGcaaaa                                                                                                                             >  3:548735/1‑150 (MQ=255)
                         cGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTTCTGCGG‑TCCGGCgaaaa                                                                                                                             >  3:621715/1‑150 (MQ=255)
                         cGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTTCTGCGG‑TACGGGgaaaa                                                                                                                             >  3:1272094/1‑150 (MQ=255)
                           ttttAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGGGATCCCGCGG‑TCCGGCaaaaa                                                                                                                             >  2:747960/1‑148 (MQ=255)
                              tAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGccc                                                                                                                        <  2:623248/150‑1 (MQ=255)
                              tAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGccc                                                                                                                        <  1:330036/150‑1 (MQ=255)
                              tAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCTGCGG‑TCCGGCAAAAAAGccc                                                                                                                        >  2:326528/1‑150 (MQ=255)
                               aaaaGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCg                                                                                                                       <  1:445595/150‑1 (MQ=255)
                                aaaGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCTGCGA‑TCCGGGCAAAAAGccc                                                                                                                        >  4:135479/1‑148 (MQ=255)
                                   gCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAg                                                                                                                  <  2:161391/151‑1 (MQ=255)
                                    ccAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGATCCTGCGA‑TCCGGCAAAAACGCCCGAGaa                                                                                                                   >  2:204390/1‑149 (MQ=255)
                                      aGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGa                                                                                                               <  3:397934/151‑1 (MQ=255)
                                      aGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGa                                                                                                               <  3:594949/151‑1 (MQ=255)
                                       gTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGAt                                                                                                              <  2:621689/151‑1 (MQ=255)
                                       gTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGAt                                                                                                              <  3:95792/151‑1 (MQ=255)
                                        tGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGGGGTCCTGCGA‑TCCGGCAAAAAACCCCGGGAAggg                                                                                                                >  4:324000/1‑146 (MQ=255)
                                        tGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGAt                                                                                                              <  1:20298/150‑1 (MQ=255)
                                         gAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCTTCGG‑TCCGGCAAAAAAACCCCGGAAGTGagc                                                                                                             >  3:1209950/1‑148 (MQ=255)
                                          aaaaTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATcgc                                                                                                           <  3:162048/151‑1 (MQ=255)
                                           aaaTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCTGCGA‑TCCGGCAAAAACGCCCGGGAAGGGATcgcc                                                                                                          >  4:410989/1‑151 (MQ=255)
                                            aaTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTTCCTCGGT‑CCCGGCAAAAA‑GCCCGGGaaagggatgcc                                                                                                          >  1:367467/1‑141 (MQ=255)
                                              tGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCTGCGG‑TCCGGCAAAAAAGCCCGAGAAGGGATCgccgcc                                                                                                       >  3:315112/1‑151 (MQ=255)
                                               ggCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCgccgccc                                                                                                      <  4:317531/151‑2 (MQ=255)
                                               ggCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCgccgcc                                                                                                       <  2:1106914/150‑1 (MQ=255)
                                               ggCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGccgccg                                                                                                      <  2:559888/151‑1 (MQ=255)
                                                 cGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTTCTGCGA‑GCCGGCAAAAACGCCCGGGAAGAGATCGccgccgcg                                                                                                    >  2:762096/1‑149 (MQ=255)
                                                  gTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGATCCTGCGA‑TCCGGCAAAAAAGCCCGAGAAGGGATCGccgccg                                                                                                      >  2:125806/1‑148 (MQ=255)
                                                    gCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGaa                                                                                                  <  3:1054326/150‑1 (MQ=255)
                                                     cccGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAg                                                                                                 <  3:1294829/150‑1 (MQ=255)
                                                      ccGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGa                                                                                               <  3:427326/151‑1 (MQ=255)
                                                          ttATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGTGACGCTGCGT‑TCCGGCCAAAAAGCCAGCGAAGGGAGCCCCGCCGCGAAggcgaa                                                                                            >  3:1380262/1‑146 (MQ=255)
                                                           tATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGCACaaa                                                                                           <  2:844836/150‑1 (MQ=255)
                                                           tATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCTGCGA‑TCCGGCAAAAAAGCCCGCGGAGTGATTGCCGCCGGGAAGGGCaaa                                                                                           >  3:234959/1‑150 (MQ=255)
                                                                gcgcGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGGGGTCCACTGAGTCCGGCAAAAAAACCAG‑GGAGGGGTCCCCGCCGCGCAGGACaa                                                                                            >  2:621412/1‑144 (MQ=255)
                                                                gcgcGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACt                                                                                         <  3:670192/147‑1 (MQ=255)
                                                                   cgATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCTGCGA‑TCCGGCAAAAACGCCCCAGAAGGGATCGCCCCCGGGAAGGACAAACTGGaaa                                                                                    >  3:1354544/1‑149 (MQ=255)
                                                                   cgATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGGGATCGCCGCCGTGGAGGACAAACTGGaaa                                                                                    >  1:150484/1‑149 (MQ=255)
                                                                    gATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACg                                                                                  <  3:442199/150‑1 (MQ=255)
                                                                    gATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCTGCGA‑TCCGGCAAAAAAGCCCGAGAAGGGATCGCCGCCGTGAAGGACAAACTGGaaa                                                                                    >  4:168608/1‑148 (MQ=255)
                                                                    gATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGGTCGCCGCCGTGAAGGACAAACTGGaaa                                                                                    >  1:172781/1‑148 (MQ=255)
                                                                        ttGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGGGAAGGACAAACTGGAAAAGCTGa                                                                              >  1:371322/1‑150 (MQ=255)
                                                                          gCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGaaaa                                                                           <  1:132588/151‑1 (MQ=255)
                                                                              aGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGGGGTCCTGCGA‑GCCCGCAAAAACACCCGGGAAGGGAATGCCCCCGGGGAGGAGCAACTGGAAACCCTGgaaa                                                                           >  2:307348/1‑147 (MQ=255)
                                                                                  ccAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCt                                                                    <  1:439896/150‑1 (MQ=255)
                                                                                  ccAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGGCAAACTGGGAAAGCTGAAAAGTAGTCt                                                                    >  3:218496/1‑150 (MQ=255)
                                                                                   cAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGTGGTCCTGCGC‑TCCGGCAAAAAAGCCCCAGAAGGGATCGCCGCCGTGAAGGGCAAACTGGAAACGCTGGAAAGGAGTCTg                                                                   >  1:93313/1‑150 (MQ=255)
                                                                                         ggCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAg                                                            <  4:322729/151‑1 (MQ=255)
                                                                                          gCCAGGGGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAgg                                                           <  3:1081747/151‑1 (MQ=255)
                                                                                           cccGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGc                                                          <  2:234933/151‑1 (MQ=255)
                                                                                              ggAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGATCCTGCGG‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAAATGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTg                                                       >  1:200543/1‑151 (MQ=255)
                                                                                                agaTGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGga                                                     <  2:566980/151‑1 (MQ=255)
                                                                                                 gaTGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGgag                                                    <  3:762131/151‑1 (MQ=255)
                                                                                                  aTGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGACCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGgag                                                    <  2:1117851/150‑1 (MQ=255)
                                                                                                     cgcCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGTTAGCCCTGGA‑TCCCGCAAAAACGCCCGCGAAGTGGCGGGCGCCCTGGAGGATAAACTGGGAACCCTCGAAAGACGGCTGCCGGAAGGCGTGggga                                                   >  2:565012/1‑148 (MQ=255)
                                                                                                       ccGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGGGATCCTGCGA‑TCCGGCAAAAAAGCCCGAGAAGTGATCGCCGCCGTGAAGGGCAAACTGGAAACGCTGAAAAGTAGTCTGCCGGGAGGCGGGGAGATAGtt                                              >  2:443901/1‑151 (MQ=255)
                                                                                                       ccGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTTCTGCGA‑TCCCGCAAAAACCCCCGAGAAGTGATCCCCGCCGGGAAGGACAAACTGGAAACGCTGAAAAGTAGGCTGCCGGAAGGCGTGGGGATAggt                                              >  1:181570/1‑149 (MQ=255)
                                                                                                         tgggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCTGCGG‑TCCGGCCAAAACGCCCGGGGAGGGATCGCCGCCGCGAAGGGCAAACTGGAAAAGCTGAAAAGAAGTCTGCCGGGAGGCGGGGGGGTAGtt                                              >  3:742859/2‑149 (MQ=255)
                                                                                                          gggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTaca                                           <  3:954874/151‑1 (MQ=255)
                                                                                                              aTTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGGGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGGGATCGCCGCCGGGAAGGACAAACTGGAAAAGCTGGAAAAAAGTCTGCCGGGAGGCGGGGAGAAAGGTacaaca                                        >  3:1114650/1‑150 (MQ=255)
                                                                                                               ttGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGCGAAGGACAAACTGGAAAAGCTGAAAAGTAGTCTGCCGGGAGGCGGGGGGATAGTTacaaaa                                        >  2:1257577/1‑147 (MQ=255)
                                                                                                                  ccGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGa                                   <  3:1292916/151‑1 (MQ=255)
                                                                                                                  ccGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGa                                   <  1:285144/151‑1 (MQ=255)
                                                                                                                   cGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGAt                                  <  4:255793/151‑1 (MQ=255)
                                                                                                                     aaCAAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATc                                 <  2:719844/150‑1 (MQ=255)
                                                                                                                       cTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATAc                                     <  2:1043761/144‑1 (MQ=255)
                                                                                                                       cTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGGGGTACTGCGA‑TCCGGCAAAAACGCCCGGGAAGGGATCGCCGCCGGGAAGGGCAAAACGGGAACGCTGAAAAGAAGTCTGCCGGGAGGGGGGGGGAAAGTTACAAAATAcagatagg                              >  3:1043807/1‑144 (MQ=255)
                                                                                                                          aaCGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTTCTGCGA‑TCCGGCAAAAACGCCCGAGAAGGGATCGCCGCCGGGAAGGACAAACTGGGAAAGCTGAAAAGAAGTCTGCCGGAAGGGGGGGAGGTAGTTACAACATACGATCGcagcc                           >  3:897408/1‑151 (MQ=255)
                                                                                                                           aCGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTACTGCGG‑TCCGGCAAAAACGCCCGAGAAGAGATCGCCGCCGGGAAGGGCAAAATGGAAACGCTGAAAAGAAGGCTGCCGGGAGGCGGGGAGATAGTTTCAACATACGAACGcagcc                           >  3:973996/1‑150 (MQ=255)
                                                                                                                             ggcgaaggcgaaGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGATAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGcagccagc                        <  3:1123906/151‑1 (MQ=255)
                                                                                                                               cgaaggcgaagTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTc                      <  2:1063339/151‑1 (MQ=255)
                                                                                                                                gaaggcgaagTGGC‑GGGCGGGGGGGGGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGGAGTCTTCCGGAAGGCGTGGGGATAGTTACAACATACGATCGCAGCCAGCTc                      >  4:17243/1‑150 (MQ=255)
                                                                                                                                        agTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACcgc             <  2:1272028/151‑1 (MQ=255)
                                                                                                                                         gTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACcgcg            <  2:527237/151‑1 (MQ=255)
                                                                                                                                         gTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACcgc             <  3:1246640/150‑1 (MQ=255)
                                                                                                                                         gTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACcgc             <  3:525537/150‑1 (MQ=255)
                                                                                                                                                ggcgggGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCg      <  3:323029/151‑1 (MQ=255)
                                                                                                                                                   ggggTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGAc    >  2:502621/1‑150 (MQ=255)
                                                                                                                                                    gggTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACaa  <  3:185040/151‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                    
AAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAA  >  NC_000913/599426‑599723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑