Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913599,5790TG44.9% 218.3 / 11.5 118V289G (GTG→GGG) cusAcopper/silver efflux system, membrane component
Reads supporting (aligned to +/- strand):  ref base T (8/57);  new base G (52/1);  total (60/58)
Fisher's exact test for biased strand distribution p-value = 1.14e-23
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

CGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACCTCA  >  NC_000913/599429‑599728
                                                                                                                                                       |                                                                                                                                                      
cGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGggtggt                                                                                                                                                        <  2:579259/151‑1 (MQ=255)
     gacgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATcc                                                                                                                                                   <  3:1378400/151‑1 (MQ=255)
      acgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCt                                                                                                                                                  <  2:32872/151‑1 (MQ=255)
         acTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCTGc                                                                                                                                                >  2:970284/1‑150 (MQ=255)
         acTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCCGc                                                                                                                                                >  2:954836/1‑150 (MQ=255)
           tttAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGGGGTcc                                                                                                                                                   >  1:24582/1‑145 (MQ=255)
            ttAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGa                                                                                                                                              <  2:94424/149‑1 (MQ=255)
             tAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGATCCTGCGA‑Tcc                                                                                                                                          >  2:670171/1‑151 (MQ=255)
              aaTCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCg                                                                                                                                         <  2:1168536/151‑1 (MQ=255)
               aTCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCgg                                                                                                                                        <  3:1098820/151‑1 (MQ=255)
               aTCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGTGAGCCTGCGA‑TACgg                                                                                                                                        >  4:285141/1‑151 (MQ=255)
                 cacaTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCa                                                                                                                                      <  2:1406362/151‑1 (MQ=255)
                  acaTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCaa                                                                                                                                     <  1:121724/151‑1 (MQ=255)
                      cGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGGTGTCCTGCGG‑TCCGGGcaaaa                                                                                                                                  >  3:548735/1‑150 (MQ=255)
                      cGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTTCTGCGG‑TCCGGCgaaaa                                                                                                                                  >  3:621715/1‑150 (MQ=255)
                      cGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTTCTGCGG‑TACGGGgaaaa                                                                                                                                  >  3:1272094/1‑150 (MQ=255)
                        ttttAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGGGATCCCGCGG‑TCCGGCaaaaa                                                                                                                                  >  2:747960/1‑148 (MQ=255)
                        ttttAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGATCCTGCGA‑TCCGGCaaaaa                                                                                                                                  >  1:205437/1‑148 (MQ=255)
                           tAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGccc                                                                                                                             <  2:623248/150‑1 (MQ=255)
                             aaaGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCTGCGA‑TCCGGGCAAAAAGccc                                                                                                                             >  4:135479/1‑148 (MQ=255)
                                gCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAg                                                                                                                       <  3:402163/151‑1 (MQ=255)
                                gCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAg                                                                                                                       <  2:161391/151‑1 (MQ=255)
                                   aGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGa                                                                                                                    <  3:397934/151‑1 (MQ=255)
                                   aGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGa                                                                                                                    <  2:1173903/151‑1 (MQ=255)
                                    gTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGAt                                                                                                                   <  2:621689/151‑1 (MQ=255)
                                    gTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGAt                                                                                                                   <  3:95792/151‑1 (MQ=255)
                                     tGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGGGGTCCTGCGA‑TCCGGCAAAAAACCCCGGGAAggg                                                                                                                     >  4:324000/1‑146 (MQ=255)
                                     tGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGAt                                                                                                                   <  1:20298/150‑1 (MQ=255)
                                     tGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATc                                                                                                                  >  2:76054/1‑151 (MQ=255)
                                      gAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCTTCGG‑TCCGGCAAAAAAACCCCGGAAGTGagc                                                                                                                  >  3:1209950/1‑148 (MQ=255)
                                       aaaaTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATcgc                                                                                                                <  3:162048/151‑1 (MQ=255)
                                        aaaTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATcgcc                                                                                                               <  1:157528/151‑1 (MQ=255)
                                        aaaTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCTGCGA‑TCCGGCAAAAACGCCCGGGAAGGGATcgcc                                                                                                               >  4:410989/1‑151 (MQ=255)
                                         aaTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTTCCTCGGT‑CCCGGCAAAAA‑GCCCGGGaaagggatgcc                                                                                                               >  1:367467/1‑141 (MQ=255)
                                           tGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCTGCGG‑TCCGGCAAAAAAGCCCGAGAAGGGATCgccgcc                                                                                                            >  3:315112/1‑151 (MQ=255)
                                            ggCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCgccgcc                                                                                                            <  2:1106914/150‑1 (MQ=255)
                                            ggCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGccgccg                                                                                                           <  2:559888/151‑1 (MQ=255)
                                              cGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTTCTGCGA‑GCCGGCAAAAACGCCCGGGAAGAGATCGccgccgcg                                                                                                         >  2:762096/1‑149 (MQ=255)
                                               gTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGATCCTGCGA‑TCCGGCAAAAAAGCCCGAGAAGGGATCGccgccg                                                                                                           >  2:125806/1‑148 (MQ=255)
                                                 gCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGaa                                                                                                       <  3:1054326/150‑1 (MQ=255)
                                                  cccGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAg                                                                                                      <  3:1294829/150‑1 (MQ=255)
                                                       ttATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGTGACGCTGCGT‑TCCGGCCAAAAAGCCAGCGAAGGGAGCCCCGCCGCGAAggcgaa                                                                                                 >  3:1380262/1‑146 (MQ=255)
                                                        tATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCTGCGA‑TCCGGCAAAAAAGCCCGCGGAGTGATTGCCGCCGGGAAGGGCaaa                                                                                                >  3:234959/1‑150 (MQ=255)
                                                             gcgcGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGGGGTCCACTGAGTCCGGCAAAAAAACCAG‑GGAGGGGTCCCCGCCGCGCAGGACaa                                                                                                 >  2:621412/1‑144 (MQ=255)
                                                             gcgcGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACt                                                                                              <  3:670192/147‑1 (MQ=255)
                                                                cgATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCTGCGA‑TCCGGCAAAAACGCCCCAGAAGGGATCGCCCCCGGGAAGGACAAACTGGaaa                                                                                         >  3:1354544/1‑149 (MQ=255)
                                                                cgATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGGGATCGCCGCCGTGGAGGACAAACTGGaaa                                                                                         >  1:150484/1‑149 (MQ=255)
                                                                cgATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGATCCTGCGA‑TCCGGCAAAAAAGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGaaa                                                                                         >  2:938745/1‑149 (MQ=255)
                                                                 gATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACg                                                                                       <  3:442199/150‑1 (MQ=255)
                                                                 gATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCTGCGA‑TCCGGCAAAAAAGCCCGAGAAGGGATCGCCGCCGTGAAGGACAAACTGGaaa                                                                                         >  4:168608/1‑148 (MQ=255)
                                                                 gATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGGTCGCCGCCGTGAAGGACAAACTGGaaa                                                                                         >  1:172781/1‑148 (MQ=255)
                                                                     ttGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGGGAAGGACAAACTGGAAAAGCTGa                                                                                   >  1:371322/1‑150 (MQ=255)
                                                                       gCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGaaaa                                                                                <  1:132588/151‑1 (MQ=255)
                                                                           aGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGGGGTCCTGCGA‑GCCCGCAAAAACACCCGGGAAGGGAATGCCCCCGGGGAGGAGCAACTGGAAACCCTGgaaa                                                                                >  2:307348/1‑147 (MQ=255)
                                                                               ccAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCt                                                                         <  1:439896/150‑1 (MQ=255)
                                                                               ccAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGGCAAACTGGGAAAGCTGAAAAGTAGTCt                                                                         >  3:218496/1‑150 (MQ=255)
                                                                               ccACATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGGAGTCTg                                                                        >  1:59061/1‑151 (MQ=255)
                                                                                cAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGTGGTCCTGCGC‑TCCGGCAAAAAAGCCCCAGAAGGGATCGCCGCCGTGAAGGGCAAACTGGAAACGCTGGAAAGGAGTCTg                                                                        >  1:93313/1‑150 (MQ=255)
                                                                                      ggCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAg                                                                 <  4:322729/151‑1 (MQ=255)
                                                                                       gCCAGGGGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAgg                                                                <  3:1081747/151‑1 (MQ=255)
                                                                                        cccGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGc                                                               <  2:234933/151‑1 (MQ=255)
                                                                                           ggAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGATCCTGCGG‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAAATGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTg                                                            >  1:200543/1‑151 (MQ=255)
                                                                                             agaTGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGga                                                          <  2:566980/151‑1 (MQ=255)
                                                                                             agaTGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGGGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTgg                                                           >  2:351696/1‑150 (MQ=255)
                                                                                             agaTGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCTTCGA‑TCCGGCAAAAAAGCCCGCGAAGGGAGCGCCGCCGCGAAGGACAAAATGGAAAAGCTGAAAAGGAGTCTGCCGGAAGGCgggg                                                           >  3:1232365/1‑150 (MQ=255)
                                                                                              gaTGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGgag                                                         <  3:762131/151‑1 (MQ=255)
                                                                                               aTGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGACCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGgag                                                         <  2:1117851/150‑1 (MQ=255)
                                                                                                  cgcCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGTTAGCCCTGGA‑TCCCGCAAAAACGCCCGCGAAGTGGCGGGCGCCCTGGAGGATAAACTGGGAACCCTCGAAAGACGGCTGCCGGAAGGCGTGggga                                                        >  2:565012/1‑148 (MQ=255)
                                                                                                    ccGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGGGATCCTGCGA‑TCCGGCAAAAAAGCCCGAGAAGTGATCGCCGCCGTGAAGGGCAAACTGGAAACGCTGAAAAGTAGTCTGCCGGGAGGCGGGGAGATAGtt                                                   >  2:443901/1‑151 (MQ=255)
                                                                                                      tgggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCTGCGG‑TCCGGCCAAAACGCCCGGGGAGGGATCGCCGCCGCGAAGGGCAAACTGGAAAAGCTGAAAAGAAGTCTGCCGGGAGGCGGGGGGGTAGtt                                                   >  3:742859/2‑149 (MQ=255)
                                                                                                       gggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTaca                                                <  3:954874/151‑1 (MQ=255)
                                                                                                           aTTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGGGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGGGATCGCCGCCGGGAAGGACAAACTGGAAAAGCTGGAAAAAAGTCTGCCGGGAGGCGGGGAGAAAGGTacaaca                                             >  3:1114650/1‑150 (MQ=255)
                                                                                                           aTTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGAACCTGCGG‑TACGGCAAAAAAGCCCCAGAAGTGATAGCCGCCGTGAAGGACAAACTGGAAACCCTGAAAAGTAGTCTGCCGGAAGGGGTGGGGATAGTTaaaaca                                             >  3:1111031/1‑150 (MQ=255)
                                                                                                            ttGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGCGAAGGACAAACTGGAAAAGCTGAAAAGTAGTCTGCCGGGAGGCGGGGGGATAGTTacaaaa                                             >  2:1257577/1‑147 (MQ=255)
                                                                                                              gCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGGGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGGAGTCTGCCGGGAGGCGTGGGGATAGTTACAACATAc                                          >  1:333285/1‑150 (MQ=255)
                                                                                                               ccGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGa                                        <  1:285144/151‑1 (MQ=255)
                                                                                                               ccGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGa                                        <  3:1021506/151‑1 (MQ=255)
                                                                                                                cGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGAt                                       <  4:255793/151‑1 (MQ=255)
                                                                                                                  aaCAAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATc                                      <  2:719844/150‑1 (MQ=255)
                                                                                                                   aCTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGc                                    <  2:1078156/151‑1 (MQ=255)
                                                                                                                    cTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATAc                                          <  2:1043761/144‑1 (MQ=255)
                                                                                                                       aaCGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTTCTGCGA‑TCCGGCAAAAACGCCCGAGAAGGGATCGCCGCCGGGAAGGACAAACTGGGAAAGCTGAAAAGAAGTCTGCCGGAAGGGGGGGAGGTAGTTACAACATACGATCGcagcc                                >  3:897408/1‑151 (MQ=255)
                                                                                                                        aCGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGGGGTCCTGCGA‑TCCGGCGAAAACGCCCGAGAAGGGATCGCCGCCGGGAAGGGCAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGGGGGGATAGGTACAACATACGATCGcagc                                 >  4:321234/1‑149 (MQ=255)
                                                                                                                        aCGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTACTGCGG‑TCCGGCAAAAACGCCCGAGAAGAGATCGCCGCCGGGAAGGGCAAAATGGAAACGCTGAAAAGAAGGCTGCCGGGAGGCGGGGAGATAGTTTCAACATACGAACGcagcc                                >  3:973996/1‑150 (MQ=255)
                                                                                                                            cgaaggcgaagTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTc                           <  2:1063339/151‑1 (MQ=255)
                                                                                                                             gaaggcgaagTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCa                          <  3:69397/151‑1 (MQ=255)
                                                                                                                             gaaggcgaagTGGC‑GGGCGGGGGGGGGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGGAGTCTTCCGGAAGGCGTGGGGATAGTTACAACATACGATCGCAGCCAGCTc                           >  4:17243/1‑150 (MQ=255)
                                                                                                                              aaggctaagTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCAt                         <  2:640072/151‑1 (MQ=255)
                                                                                                                              aaggcgaagTGGC‑GGGCGGGGGGGGGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGGGATCGCCGCCGCGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGGGGGGATAGTTACAACATACGATCGCAGCCAGCTCa                          >  3:1349482/1‑150 (MQ=255)
                                                                                                                               aggcgaagTGGC‑GGGCGGGGTGGGGATCCCGCGA‑TTCCGCAAAAACACCCGAGAAGGTATTGGCGCCGAGAAGGACCAAATGGAAACGCTGAAAAGAAGTCTGCCGGAAGGCGGGGGGAAAATTTCAACATAAGAACGCAGCCCGGTCAtt                        >  3:55949/1‑151 (MQ=255)
                                                                                                                                     agTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACcgc                  <  2:1272028/151‑1 (MQ=255)
                                                                                                                                      gTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACcgcg                 <  2:527237/151‑1 (MQ=255)
                                                                                                                                      gTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACcgc                  <  3:525537/150‑1 (MQ=255)
                                                                                                                                      gTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACcgc                  <  3:1246640/150‑1 (MQ=255)
                                                                                                                                             ggcgggGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCg           <  3:323029/151‑1 (MQ=255)
                                                                                                                                                ggggTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGGGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGAc         >  2:243074/1‑150 (MQ=255)
                                                                                                                                                ggggTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGAc         >  2:502621/1‑150 (MQ=255)
                                                                                                                                                 gggTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACaa       <  3:185040/151‑1 (MQ=255)
                                                                                                                                                     ggtgATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACCt    <  2:258806/150‑1 (MQ=255)
                                                                                                                                                      gtgATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACCTCa  <  3:326558/151‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                      
CGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGA‑TCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACCTCA  >  NC_000913/599429‑599728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑