Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,376,5050AC42.7% 188.8 / 32.9 104T158P (ACC→CCC) ycjRputative TIM alpha/beta barrel enzyme
Reads supporting (aligned to +/- strand):  ref base A (48/11);  new base C (0/44);  total (48/56)
Fisher's exact test for biased strand distribution p-value = 5.14e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

TACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAAT‑GGTGAGTGATTCCCTGCGCGTACTGG‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATATT  >  NC_000913/1376359‑1376651
                                                                                                                                                           |                                                                                                                                                  
tACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAAT‑GGTGAGTGATTCCCTGCGCGTACTGG‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACAccc                                                                                                                                                 >  4:167065/1‑150 (MQ=255)
 aCCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAAT‑GGTGAGTGATTCCCTGCGCGTACTGG‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTc                                                                                                                                               >  2:1069782/1‑151 (MQ=255)
  ccgccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAAT‑GGTGAGTGATTCCCTGCGCGTACTGG‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAaca                                                                                                                                                    <  2:91653/145‑1 (MQ=255)
  ccgccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAAT‑GGTGAGTGATTCCCTGCGCGTACTGG‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTc                                                                                                                                               <  3:1373208/150‑1 (MQ=255)
  ccgccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAAT‑GGTGAGTGATTCCCTGCGCGTACTGG‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTc                                                                                                                                               <  3:962647/150‑1 (MQ=255)
    gccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAAT‑GGTGAGTGATTCCCTGCGCGTACTGG‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGcc                                                                                                                                            <  2:457869/151‑1 (MQ=255)
     ccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAAT‑GGTGAGTGATTCCCTGCGCGTACTGG‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCg                                                                                                                                           >  2:253808/1‑151 (MQ=255)
     ccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAAT‑GGTGAGTGATTCCCTGCGCGTACTGG‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCg                                                                                                                                           >  2:1216754/1‑151 (MQ=255)
     ccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAAT‑GGTGAGTGATTCCCTGCGCGTACTGG‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCg                                                                                                                                           <  2:37159/151‑1 (MQ=255)
      cgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAAT‑GGTGAGTGATTCCCTGCGCGTACTGG‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCg                                                                                                                                           >  1:274620/1‑150 (MQ=255)
           aCCTCGCCGCGTAGCCTGGACGGCGACCGCAAAAT‑GGTGAGTGATTCCCTGCGCGTACTGG‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTc                                                                                                                                               <  1:43899/141‑1 (MQ=255)
           aCCTCGCCGCGTAGCCTGGACGGCGACCGCAAAAT‑GGTGAGTGATTCCCTGCGCGTACTGG‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTc                                                                                                                                               >  4:43899/1‑141 (MQ=255)
           aCCTCGCCGCGTAGCCTGGACGGCGACCGCAAAAT‑GGTGAGTGATTCCCTGCGCGTACTGG‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCGATGccc                                                                                                                                     >  1:172018/1‑151 (MQ=255)
            ccTCGCCGCGTAGCCTGGACGGCGACCGCAAAAT‑GGTGAGTGATTCCCTGCGCGTACTGG‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCGATGccc                                                                                                                                     <  4:172016/150‑1 (MQ=255)
               cgccgcGTAGCCTGGACGGCGACCGCAAAAT‑GGTGAGTGATTCCCTGCGCGTACTGG‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCGACACCCTCGCCGATGCccgc                                                                                                                                   <  4:327312/149‑1 (MQ=255)
               cgccgcGTAGCCTGGACGGCGACCGCAAAAT‑GGTAAGTGATTCCCTGCGCGTACTGG‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCGATGCccgcc                                                                                                                                  <  2:354457/150‑1 (MQ=255)
                gccgcGTAGCCTGGACGGCGACCGCAAAAT‑GGTGAGTGATTCCCTGCGCGTACTGG‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGt                                                                                                                                >  2:255823/1‑151 (MQ=255)
                gccgcGTAGCCTGGACGGCGACCGCAAAAT‑GGTGAGTGATTCCCTGCGCGTACTGG‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGt                                                                                                                                >  3:179832/1‑151 (MQ=255)
                       aGCCTGGACGGCGACCGCAAAAT‑GGTGAGTGATTCCCTGCGCGTACTGG‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACAtcg                                                                                                                         >  3:876055/1‑151 (MQ=255)
                           tGGACGGCGACCGCAAAAT‑GGTGAGTGATTCCCTGCGCGTACTGG‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACAtcgtcg                                                                                                                      >  2:1320873/1‑150 (MQ=255)
                            gggcGGCGCCCGAAAAAT‑GGGGAGTGTTCCCCTGCGCGTACGGG‑AACGGGTCGCCGCGCGTACCGGAC‑CC‑GTGGTGTACCTCGACCCGTAAAC‑CC‑GC‑T‑AT‑CAGGATAATATGATCAACCCCCGCGCCGATGCCCGCCGTTACATCGTCGa                                                                                                                     <  2:1192119/147‑1 (MQ=255)
                            ggccGGCGCCCGAAAAAG‑GGTGAGTGTTCCCCTGCGCTTACTGG‑AACGGGTCGCCGCGCGT‑CCGGAACCC‑GGGGTTTATCTCGACCCGTTAAC‑CC‑GC‑T‑AT‑CAGGATAATATGATCAACCCCCCCGCCGATGCCCGCCGTTACAtcgtcg                                                                                                                      <  2:337717/146‑1 (MQ=255)
                            ggACGGCGACCCAAAAAT‑GGTGGGTATTCCCCTGC‑TGTTGTGGAAACGGGTCGCCGCGCGTACGGGCA‑CC‑GGGTTGTATCTGGAACCTTTAAC‑CC‑GT‑T‑AC‑CAGGATAATGTGACCAACCCCCTCCCCGATGCCCGCCGTTACAtcg                                                                                                                         <  4:406602/146‑1 (MQ=255)
                               cGGCGACCGCAAAAT‑GGTGAGTGATTCCCTGCGCGTACTGG‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAAc                                                                                                                 >  3:207055/1‑151 (MQ=255)
                               cGGCGACCGCAAAAT‑GGTGAGTGATTCCCTGCGCGTACTGG‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAAc                                                                                                                 >  2:75238/1‑151 (MQ=255)
                                ggcgcccaaaaaaT‑GGTGTGTGATCCCGGGCGCGTGCTGG‑AACTGGTCGCGGCGCGTCCCGAAA‑CT‑GTGGTGTCTCCCGACCCTTAAAC‑CC‑GC‑T‑AT‑AAGGATTTTATGACCACCCCCGCCGCCGATGCCCGCCGTTACATCGTCGAAAAc                                                                                                                 <  2:981707/141‑1 (MQ=255)
                                ggCGACCAAAAAAT‑GGTGAGTGATTCCCGGCGCGTAGGGG‑ACCGGGTCGCCGCGCGTCCGGGAC‑CC‑GTGGTTTATCTC‑AACCCTTAAACCC‑CC‑T‑AT‑CGGGATAATATAATAAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAAc                                                                                                                 <  2:912451/150‑1 (MQ=255)
                                  gcgcccaaaaaTGGGTGATTGATCCCCTGCGCGTACTGG‑AACAGGTCGCCGCGCGTCCGGGAC‑CC‑GTGGTGTATCTCGACCCATTAAA‑CC‑GC‑T‑AC‑CAGGATCATATGACCAACCCCCTCACCAATGCCCGCCGTTACATCGTCGAAAACGa                                                                                                               <  3:202672/144‑1 (MQ=255)
                                   gACCGCAAAAT‑GGTGAGTGATTCCCTGCGCGTACTGG‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCGATGCGCGCCGTTACATCGTCGAAAACGATc                                                                                                             >  3:297602/1‑151 (MQ=255)
                                   gACCGCAAAAT‑GGTGAGTGATTCCCTGCGCGTACTGG‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATc                                                                                                             >  2:145110/1‑151 (MQ=255)
                                    cccGCAAAAT‑GGTGAGTGATTCCCTGCGCGTACTGG‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCt                                                                                                            >  2:1063236/2‑151 (MQ=255)
                                     cccaaaaaT‑GGTGAGGGATCCCCGGCGCGTACGGA‑AACGGGTCGCCGCGCGTACGGAAC‑CC‑GGGGTGTATCTCGACCCTTTAAC‑CC‑GC‑T‑AT‑CAGGATCATATAATCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCtt                                                                                                           <  4:84238/147‑1 (MQ=255)
                                     ccGAAAAAG‑GGTGAGTTTTCCCCTGCGCGTACGGG‑AACGGGTCGCCGCGCGTCCCGAAC‑CC‑GTGGTGTATCTCGACCCTTAAAC‑CC‑GC‑T‑AT‑CAGGATAATATGATCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCt                                                                                                            <  2:87743/150‑1 (MQ=255)
                                      cgaaaaaT‑GGTGAGTGTTCCCTTGCGCTTCCTGG‑AAGAGGTCCCGGCGCGTCCCGGAA‑CG‑GGGGTATTTCTGGAACCTTAAAC‑CC‑GT‑T‑AT‑AGGGATCATATGAAAAACCCCCTCGCGAAGGCCCGCCGTTACATCGTCGAAAACGa                                                                                                               <  2:878828/143‑1 (MQ=255)
                                      cgaaaaaT‑GGTGAGTGATCCCCGCCGCGTACGGA‑AACGGGCCCCCCCGCGTCCCGGAC‑CC‑GGGGTGTCTCTCGACCCTTAAAC‑CC‑GC‑T‑CT‑CAGAATAATATGAAAACCCCCCCCCCCGATGCCCGCCGTTACATCGTCGAAAACGa                                                                                                               <  3:627089/143‑1 (MQ=255)
                                        aaaagT‑GGGGAGTGTTCCCCTGCGCGTACTGG‑AACGGGTCGCCGCGCGTCCCGGAC‑CC‑GTGTTGTATCCCGAACCGTAAAC‑CC‑TC‑T‑AT‑CAGGATAATATGAAAAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTaaa                                                                                                        <  3:1143132/150‑1 (MQ=255)
                                         aaagT‑GGTGAGTGATTCCCGGCGCGTACGGG‑AACAGGTCGCCGCGCGTACCGGAC‑CC‑GTGGTTTATCTCGACCCTTTAAC‑CC‑GT‑T‑AT‑CAGGATCATAGGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTaaa                                                                                                        <  2:291465/150‑1 (MQ=255)
                                          aagagggtgggtgATTCCCGGCGGGTAGGGG‑AACGGGCCGCCGGGCGTCCCGGAC‑CG‑GGGTTGTACCCCGAACCGTTAAC‑CC‑GT‑T‑AC‑AAGGATAAAATGACCACCCCCCCCGCGGATGCCCGCCGTTACATCGTCGAAAACGATCTTaaa                                                                                                        <  4:91454/145‑1 (MQ=255)
                                           aaagggggagtacccccccGCGCGCACGGG‑AACCGGCCGCCGCGCGCCCCGGAC‑CA‑GTGGTGTATCGC‑AACCCTTCAA‑CCAGC‑G‑CG‑ACGGATCATATGAACAACCCCCCCCCGGAGGCCCGCCGTTACATCGTCGAAAACGATCTTAAACa                                                                                                      <  3:338936/136‑1 (MQ=255)
                                            aatgtggagtgtTCCCCTGCGGGTGCGGG‑AACAGGTCGCCGCGCGT‑CCGGAA‑CCGGTGTTGAATCACGAACCG‑TAAA‑CC‑CC‑T‑TTACAGGATAATATGACCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACAt                                                                                                     <  2:1135009/145‑1 (MQ=255)
                                               ggtgggtgATTCCCTGCGCGTACTGC‑ACCGGGTCGCCGCGCTTCCCGGAA‑CC‑GTGGTTTA‑CCCCAACCCTTAAACCC‑GT‑T‑AT‑CAGGACTGTATGTTAAACCCCCTCGCAGATCCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTa                                                                                                  <  2:975396/151‑1 (MQ=255)
                                               ggTGAGTGTTTCCCTGCGCGTACGGG‑AACGGGCCGCCGCGGGTCCCGAAA‑CG‑GTGGTGTATCCCGACCCTTAAAC‑CC‑GT‑T‑AT‑CAGGATAAAATAACAAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACAt                                                                                                     <  4:437669/148‑1 (MQ=255)
                                                 tgagtgaTTCCCTGCGCGTACTGG‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTAc                                                                                                 >  2:166145/1‑150 (MQ=255)
                                                  gagtgaTCCCCTGCGCGTACGGG‑AACGGGTCGCCGCGCGTCCGGGAC‑CC‑GGGGTTTATCTCAACCCGTTAAC‑CC‑GT‑T‑AT‑CAGGATCATATGACAAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACa                                                                                                <  2:151801/150‑1 (MQ=255)
                                                     tgaTTCCCTGCGCGTACTGG‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGAtt                                                                                            >  2:80240/1‑151 (MQ=255)
                                                     tgaTTCCCTGCGCGTACTGG‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGAtt                                                                                            >  3:1002424/1‑151 (MQ=255)
                                                          cccTGCGCGTACGGG‑AACGGGTCGCCGCGCGTCCCGGAC‑CC‑GTGTTGTATCTCGACCCGTTAAC‑CC‑GT‑T‑AT‑CAGGATCATATGATCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCgg                                                                                       <  1:87026/151‑1 (MQ=255)
                                                           ccTGCGCGTACTGG‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGTTCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGc                                                                                      >  1:302321/1‑151 (MQ=255)
                                                               cgcgTACTGG‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGAtt                                                                                  >  3:629537/1‑151 (MQ=255)
                                                                 gcgtagggaaaCAGGCCGCCGCGCGTACGGGAC‑CC‑GTGTTTTATCTC‑AACCCTTAAACCC‑GT‑T‑AA‑AAGAATAATATAAACAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGAttt                                                                                 <  3:1273298/142‑1 (MQ=255)
                                                                  gTACTGG‑AATAGGCCGCCGCGCGTACCGTAT‑CC‑GTGTTGTTTCCCGATCTTTTAAC‑CC‑GC‑T‑AT‑CAGGTTGATATAATCAACCCCCTCTCCGGTGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCg                                                                                     <  2:120809/145‑1 (MQ=255)
                                                                     aatgaaaggggTCGCCGCGCGTCCCGAAC‑CC‑GTGGTTTTCCTCAACCCGTAAAC‑CC‑GT‑T‑AT‑AAGGATCATAGAACAAACCCCCCCGCCGATCCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGAtt                                                                                            <  3:24976/127‑1 (MQ=255)
                                                                       gg‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATcac                                                                          >  1:292617/1‑151 (MQ=255)
                                                                        g‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATcac                                                                          >  3:1143565/1‑150 (MQ=255)
                                                                         aaaCATGTCGCCGCGCGTACCGGAC‑CC‑GTGGTGTACCTCGACCCTTTAAC‑CC‑GC‑T‑AT‑CAGAATCATATTATCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATca                                                                           <  1:380230/148‑1 (MQ=255)
                                                                          aaCAGGTCGCCGCGCGTACCGGAC‑CG‑GTGTTGTATCTCAACCCGTTAAC‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACCCCCCCCCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTAt                                                                             <  1:197578/146‑1 (MQ=255)
                                                                              ggcccccccgccccccGGAC‑CC‑GGGGTGTACCTC‑AATCCTTAAA‑CC‑CCTT‑AC‑GAGGTTCATATGATCACCCCCCTCGCCGACTCCCGCCGTTACCTCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGaa                                                                     <  4:106521/136‑1 (MQ=255)
                                                                              ggTGGCCGCGCGTCCGGGAC‑CC‑GTGGTTTACCCCGACCCGTAAAC‑CC‑GC‑T‑AG‑CAGGATAATATAATCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGa                                                                      <  3:204055/149‑1 (MQ=255)
                                                                                      gcgcttccGGAA‑CCGGTGGTGTATCTCG‑ACCGTTAAA‑CC‑CC‑TAAT‑CAGGATCATATGACAAACCCCCCCGCCGTTGACCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaa                                                              <  1:90119/144‑1 (MQ=255)
                                                                                      cgcgTCCCGGAC‑CC‑GGGGTTTATCCCGACCCGTTAAC‑CC‑GC‑T‑AT‑CAGGATCATATAACAAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaag                                                             <  2:101480/150‑1 (MQ=255)
                                                                                      cgcgTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCga                                                               >  2:9715/1‑148 (MQ=255)
                                                                                         gtccgggAA‑CC‑GTGGTTTATCTCGACCCTTTAAC‑CC‑GC‑T‑AT‑CAGAATCATATGATCACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaagaa                                                           <  1:478858/144‑1 (MQ=255)
                                                                                            cgcgggaccggttgtgtATCTC‑AACCCTTAAACCC‑TT‑T‑AT‑CAGAATAATATCACCACCCCCCTCGCCGAGGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaagaa                                                           <  3:828585/135‑1 (MQ=255)
                                                                                            ccGGAC‑CG‑GTGGTTTATCCGGACCTGTAAAC‑CC‑GC‑T‑AT‑AAGGTTAATATGATCACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATaa                                                      <  4:454892/151‑1 (MQ=255)
                                                                                              ggAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAAcc                                                    >  3:261411/1‑151 (MQ=255)
                                                                                                aa‑CC‑GTGGTGTATCTCGACCCGTAAAC‑CC‑TT‑T‑AT‑CAGGATCATATGATCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCt                                                   <  2:637765/150‑1 (MQ=255)
                                                                                                aa‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CGGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTg                                                  >  4:95554/1‑151 (MQ=255)
                                                                                                aa‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTg                                                  >  2:704693/1‑151 (MQ=255)
                                                                                                  accggtgttttATCCCGACCCTTAAAC‑CC‑GC‑T‑AT‑AAGGATCATTTGATCAACCCCCTCGCGGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCt                                                   <  3:54054/140‑1 (MQ=255)
                                                                                                   cc‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCGATGCCCACCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGgc                                                >  1:319160/1‑151 (MQ=255)
                                                                                                          tttATCCCGACCCGTTAAC‑CC‑GC‑T‑AC‑CAGAATAATATAATCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAgg                                           <  3:1069828/148‑1 (MQ=255)
                                                                                                              tctcGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTg                                      >  2:1304471/1‑151 (MQ=255)
                                                                                                               ctgaaccctttAAA‑CC‑GC‑T‑AT‑AGGGATAAAATAACCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAgg                                           <  4:345512/136‑1 (MQ=255)
                                                                                                                 caaaCCTTAAAC‑CC‑GC‑T‑AT‑CAGGATAATATGAACACCCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCAt                                   <  3:75248/149‑1 (MQ=255)
                                                                                                                 cGAACCTTTAAC‑CC‑GC‑T‑AT‑CAGAATCATATGACCAACACCCTCGCCGAGGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGgcg                                         <  2:629511/145‑1 (MQ=255)
                                                                                                                  gAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATg                                  >  2:919690/1‑151 (MQ=255)
                                                                                                                   aaCCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATg                                  >  1:370656/1‑150 (MQ=255)
                                                                                                                    aCCGTAAAA‑CC‑GT‑T‑AT‑CAGAATCATATGAAAAACCCCCCCCCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCa                                    <  3:1067301/147‑1 (MQ=255)
                                                                                                                    aCCCTTAAACCC‑GC‑T‑AT‑CAGAATAATATGACCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCt                                       <  2:876020/145‑1 (MQ=255)
                                                                                                                     cctttAAA‑CC‑CC‑T‑AT‑AAGTATAATATGATCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATg                                  <  3:50599/145‑1 (MQ=255)
                                                                                                                      cGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCGATGCCCACCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACaa                              <  4:319157/151‑1 (MQ=255)
                                                                                                                        ttAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAAcc                            >  3:281294/1‑151 (MQ=255)
                                                                                                                          aaa‑CC‑GT‑T‑AT‑AAGAATAATAAGAACACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACcgc                          <  4:63238/151‑1 (MQ=255)
                                                                                                                              cc‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGAc                       >  1:428259/1‑151 (MQ=255)
                                                                                                                                 gC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCt                     >  2:907921/1‑151 (MQ=255)
                                                                                                                                  c‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTg                    >  1:35287/1‑151 (MQ=255)
                                                                                                                                      aT‑CAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGc                   >  3:351803/1‑150 (MQ=255)
                                                                                                                                       t‑CAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTc                 >  1:336973/1‑151 (MQ=255)
                                                                                                                                           ggATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGt              >  2:1291110/1‑151 (MQ=255)
                                                                                                                                              tCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAt           >  3:1208708/1‑151 (MQ=255)
                                                                                                                                                atatGATCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAtgt         <  1:408859/151‑1 (MQ=255)
                                                                                                                                                atatGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAtgt         >  4:156772/1‑151 (MQ=255)
                                                                                                                                                atatGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAtgt         >  2:28453/1‑151 (MQ=255)
                                                                                                                                                  atGATCACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCt                     <  3:80252/137‑1 (MQ=255)
                                                                                                                                                   taacaaaCCCCCTCGCCGATCCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCt                     <  3:151172/131‑1 (MQ=255)
                                                                                                                                                   tGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGc       >  3:617914/1‑150 (MQ=255)
                                                                                                                                                   tGACCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAtgtg        <  2:1002382/149‑1 (MQ=255)
                                                                                                                                                    gATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCat     >  3:1229223/1‑151 (MQ=255)
                                                                                                                                                      tCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCatat   >  3:1352882/1‑151 (MQ=255)
                                                                                                                                                        aaCACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATAtt  >  3:58060/1‑150 (MQ=255)
                                                                                                                                                           |                                                                                                                                                  
TACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAAT‑GGTGAGTGATTCCCTGCGCGTACTGG‑AACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CC‑GC‑T‑AT‑CAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATATT  >  NC_000913/1376359‑1376651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑