Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,114,622 A→G S320P (TCC→CCC)  glpX ← fructose 1,6‑bisphosphatase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,114,6220AG99.2% 472.3 / ‑3.9 122S320P (TCC→CCC) glpXfructose 1,6‑bisphosphatase II
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (60/61);  total (61/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

Only 100 of 125 total aligned reads displayed.

TTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTATCGCTGCGCGCCATAT  >  NC_000913/4114475‑4114768
                                                                                                                                                   |                                                                                                                                                  
ttttCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGGACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCt                                                                                                                                                  <  2:249695/150‑1 (MQ=255)
  ttCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGa                                                                                                                                                <  4:362640/150‑1 (MQ=255)
  ttCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGa                                                                                                                                                >  1:393579/1‑150 (MQ=255)
    ctcCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATg                                                                                                                                             >  2:770653/1‑151 (MQ=255)
     tcCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATgc                                                                                                                                            >  3:657066/1‑151 (MQ=255)
      ccTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATg                                                                                                                                             <  4:219775/149‑1 (MQ=255)
       cTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATgcgg                                                                                                                                          >  2:1238762/1‑151 (MQ=255)
         gTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATgcggcg                                                                                                                                        >  2:280675/1‑151 (MQ=255)
              gattgaCTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATgg                                                                                                                                   >  4:328100/1‑151 (MQ=255)
              gattgaCTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATg                                                                                                                                    <  2:442672/150‑1 (MQ=255)
              gattgaCTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATg                                                                                                                                    <  2:692571/150‑1 (MQ=255)
                   aCTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGt                                                                                                                              >  1:16415/1‑151 (MQ=255)
                       gCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACt                                                                                                                          >  3:569107/1‑151 (MQ=255)
                       gCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACt                                                                                                                          <  1:470070/151‑1 (MQ=255)
                        ccTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACtt                                                                                                                         <  3:135822/151‑1 (MQ=255)
                             tattCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCcgc                                                                                                                    >  4:464151/3‑151 (MQ=255)
                               ttCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCgg                                                                                                                  >  2:861732/1‑151 (MQ=255)
                               ttCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCgg                                                                                                                  >  4:34656/1‑151 (MQ=255)
                                tCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCcgcg                                                                                                                   <  3:811561/149‑1 (MQ=255)
                                 cGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGAt                                                                                                                >  3:1146429/1‑151 (MQ=255)
                                 cGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGAt                                                                                                                >  2:1397656/1‑151 (MQ=255)
                                     ctcCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATcag                                                                                                             >  4:68095/1‑150 (MQ=255)
                                       ccGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATcagcag                                                                                                          <  2:611645/151‑1 (MQ=255)
                                       ccGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATcagc                                                                                                            >  3:6786/1‑149 (MQ=255)
                                       ccGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATcag                                                                                                             <  2:748187/148‑1 (MQ=255)
                                       ccGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATca                                                                                                              <  4:237802/147‑1 (MQ=255)
                                          aaCAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGt                                                                                                       >  2:950442/1‑151 (MQ=255)
                                           aCAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGtt                                                                                                      >  1:130962/1‑151 (MQ=255)
                                           aCAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGtt                                                                                                      >  2:368010/1‑151 (MQ=255)
                                           aCAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGtt                                                                                                      >  2:660045/1‑151 (MQ=255)
                                             aaaaaaaTTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTc                                                                                                    >  3:1386936/1‑151 (MQ=255)
                                             aaaaaaaTTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTc                                                                                                    >  1:19488/1‑151 (MQ=255)
                                              aaaaaaTTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCg                                                                                                   <  3:1161243/151‑1 (MQ=255)
                                                     tCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTc                                                                                             <  2:1327019/150‑1 (MQ=255)
                                                     tCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTc                                                                                             <  4:330920/150‑1 (MQ=255)
                                                        aGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTcgcg                                                                                          >  3:750277/1‑150 (MQ=255)
                                                        aGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTcgcg                                                                                          >  2:877076/1‑150 (MQ=255)
                                                        aGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGa                                                                                         <  2:1302125/151‑1 (MQ=255)
                                                           cccGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGata                                                                                       <  2:459267/150‑1 (MQ=255)
                                                             cGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTg                                                                                    <  1:174851/151‑1 (MQ=255)
                                                             cGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTg                                                                                    >  3:1102044/1‑151 (MQ=255)
                                                               aaGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGcc                                                                                  >  2:303026/1‑151 (MQ=255)
                                                                aGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCt                                                                                 >  4:412502/1‑151 (MQ=255)
                                                                  gACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCttt                                                                               <  2:984641/151‑1 (MQ=255)
                                                                  gACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCttt                                                                               <  2:1212680/151‑1 (MQ=255)
                                                                   aCTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTg                                                                              <  3:159781/151‑1 (MQ=255)
                                                                   aCTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTg                                                                              <  3:708373/151‑1 (MQ=255)
                                                                    cTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGc                                                                             >  3:447205/1‑151 (MQ=255)
                                                                      ggAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGTTCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCgg                                                                           <  3:257760/151‑1 (MQ=255)
                                                                       gAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGc                                                                          <  2:1297143/151‑1 (MQ=255)
                                                                        aaGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCt                                                                         <  1:315796/151‑1 (MQ=255)
                                                                         aGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTa                                                                        <  2:1007935/151‑1 (MQ=255)
                                                                         aGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTa                                                                        <  4:266838/151‑1 (MQ=255)
                                                                           gCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAAt                                                                      >  2:1312905/1‑151 (MQ=255)
                                                                             tCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGatat                                                                                      <  3:1326483/133‑1 (MQ=255)
                                                                               aaTCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCt                                                                  >  2:629795/1‑151 (MQ=255)
                                                                                aTCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCtt                                                                 <  3:388520/151‑1 (MQ=255)
                                                                                aTCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGAAGTCGCGATAGTGCCTTTGCGGCTAATGCCt                                                                  >  3:656533/1‑150 (MQ=255)
                                                                                 tCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTc                                                                >  3:1038811/1‑151 (MQ=255)
                                                                                 tCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTc                                                                >  4:172614/1‑151 (MQ=255)
                                                                                   gATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCca                                                              <  3:637069/151‑1 (MQ=255)
                                                                                    atcaaatcaaTCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTcc                                                               >  1:350316/1‑149 (MQ=255)
                                                                                    atcaaatcaaTCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCcag                                                             <  3:507598/151‑1 (MQ=255)
                                                                                      caaatcaaTCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCcagca                                                           <  3:492050/151‑1 (MQ=255)
                                                                                      caaatcaaTCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCcagc                                                            <  3:136006/150‑1 (MQ=255)
                                                                                       aaatcaaTCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCcagcag                                                          <  2:193871/151‑1 (MQ=255)
                                                                                        aatcaatcAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCcagc                                                            >  4:327017/1‑148 (MQ=255)
                                                                                        aatcaatcAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCcagc                                                            >  2:273589/1‑148 (MQ=255)
                                                                                        aatcaatcAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGa                                                         <  3:1238821/151‑1 (MQ=255)
                                                                                         atcaatcaGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCcagc                                                            >  4:466780/1‑147 (MQ=255)
                                                                                           caatcaGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATc                                                       <  2:858063/150‑1 (MQ=255)
                                                                                           caatcaGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCg                                                      <  3:723650/151‑1 (MQ=255)
                                                                                            aatcaGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATcc                                                      <  3:1065866/150‑2 (MQ=255)
                                                                                            aatcaGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGc                                                     >  4:259414/1‑151 (MQ=255)
                                                                                              tcaGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCt                                                   >  4:177922/1‑151 (MQ=255)
                                                                                                 gagGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTg                                                >  3:889114/1‑151 (MQ=255)
                                                                                                    gATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTa                                             <  2:1146373/151‑1 (MQ=255)
                                                                                                       gtgCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAAt                                           >  2:198705/1‑150 (MQ=255)
                                                                                                       gtgCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATa                                          <  3:983416/151‑1 (MQ=255)
                                                                                                           aCCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCgg                                      <  1:56054/151‑1 (MQ=255)
                                                                                                             cTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTg                                    >  2:671927/1‑151 (MQ=255)
                                                                                                             cTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTg                                    >  3:295386/1‑151 (MQ=255)
                                                                                                               gCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTgg                                   <  2:795885/150‑1 (MQ=255)
                                                                                                                cATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCa                                 <  2:750245/151‑1 (MQ=255)
                                                                                                                 aTTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGc                                  >  3:996199/1‑149 (MQ=255)
                                                                                                                    tCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCagag                              <  1:8467/150‑1 (MQ=255)
                                                                                                                     ccGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAgaga                             <  2:569076/150‑1 (MQ=255)
                                                                                                                     ccGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGagaa                            <  3:914370/151‑1 (MQ=255)
                                                                                                                                cGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTa                  >  3:787059/1‑150 (MQ=255)
                                                                                                                                cGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTAt                 >  2:593463/1‑151 (MQ=255)
                                                                                                                                  aTCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTATCg               >  2:344852/1‑151 (MQ=255)
                                                                                                                                     cAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTATCGCTg            >  4:366448/1‑151 (MQ=255)
                                                                                                                                     cAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTATCGCTg            <  2:669579/151‑1 (MQ=255)
                                                                                                                                       gATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTATCGCTgcg          <  2:1386867/151‑1 (MQ=255)
                                                                                                                                        aTAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTATCGCTgcgc         <  4:460779/151‑1 (MQ=255)
                                                                                                                                        aTAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTATCGCTgcg          >  3:1007557/1‑150 (MQ=255)
                                                                                                                                        aTAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTATCGCTgc           >  1:307392/1‑149 (MQ=255)
                                                                                                                                             ggatgggCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTATCGCTGCGCGCCa     <  2:1038764/150‑1 (MQ=255)
                                                                                                                                              gatgggCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTATCGCTGCGCGCCat    >  4:312771/1‑150 (MQ=255)
                                                                                                                                               atgggCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTATCGCTGCGCGCCatat  <  3:660067/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTATCGCTGCGCGCCATAT  >  NC_000913/4114475‑4114768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: