BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,180,463	T→C	A156A (GCT→GCC)	rplJ →	50S ribosomal subunit protein L10

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,180,463	0	T	C	100.0%	488.7 / NA	129	A156A (GCT→GCC)	rplJ	50S ribosomal subunit protein L10
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (67/62); total (67/62)

Only 100 of 129 total aligned reads displayed.

AAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCATTGAAGCAGTTGCAGCTATGTCTGTA  >  NC_000913/4180320‑4180610

   gCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTAcgcg                                                                                                                                           <  3:756169/151‑1 (MQ=255)

   gCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTAcgcg                                                                                                                                           >  2:167789/1‑151 (MQ=255)

      gCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATg                                                                                                                                        <  3:1241658/151‑1 (MQ=255)

        tGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGc                                                                                                                                       >  4:342008/1‑150 (MQ=255)

        tGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCg                                                                                                                                      >  3:498516/1‑151 (MQ=255)

        tGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCg                                                                                                                                      >  3:1091350/1‑151 (MQ=255)

        tGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCg                                                                                                                                      >  2:434140/1‑151 (MQ=255)

          ccTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGaa                                                                                                                                    <  3:785219/151‑1 (MQ=255)

          ccTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGaa                                                                                                                                    <  3:929381/151‑1 (MQ=255)

           cTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGaa                                                                                                                                    >  2:239737/1‑150 (MQ=255)

              tGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAaaga                                                                                                                                 <  2:537224/150‑1 (MQ=255)

              tGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAaaga                                                                                                                                 >  3:229433/1‑150 (MQ=255)

              tGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAaaga                                                                                                                                 <  3:1331505/150‑1 (MQ=255)

              tGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAaaga                                                                                                                                 <  3:1177778/150‑1 (MQ=255)

               gAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAaagaa                                                                                                                                <  3:1262455/150‑1 (MQ=255)

               gAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAaagaa                                                                                                                                <  3:732834/150‑1 (MQ=255)

                aaGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAgc                                                                                                                              <  2:320830/151‑1 (MQ=255)

                    tGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAgctgct                                                                                                                          >  2:1098885/1‑151 (MQ=255)

                    tGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAgctgc                                                                                                                           >  3:214977/1‑150 (MQ=255)

                     gAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAgctgct                                                                                                                          <  1:464925/150‑1 (MQ=255)

                     gAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCtt                                                                                                                         <  3:1063333/151‑1 (MQ=255)

                        cTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAAt                                                                                                                      >  2:922655/1‑151 (MQ=255)

                          gATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGaa                                                                                                                                    <  3:583730/135‑1 (MQ=255)

                          gATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCg                                                                                                                    <  3:499948/151‑1 (MQ=255)

                          gATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCg                                                                                                                    >  3:715771/1‑151 (MQ=255)

                               cGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGtt                                                                                                               >  3:562779/1‑151 (MQ=255)

                               cGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGtt                                                                                                               >  3:964752/1‑151 (MQ=255)

                                ggCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTa                                                                                                              <  2:1089156/151‑1 (MQ=255)

                                       cAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCttttt                                                                                                       >  1:453791/1‑151 (MQ=255)

                                       cAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCtt                                                                                                          <  2:334762/148‑1 (MQ=255)

                                          aTCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAAc                                                                                                    >  2:125742/1‑151 (MQ=255)

                                          aTCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAAc                                                                                                    <  2:323590/151‑1 (MQ=255)

                                            cGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACg                                                                                                   <  3:611620/150‑1 (MQ=255)

                                            cGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGc                                                                                                  <  3:406005/151‑1 (MQ=255)

                                             gACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGc                                                                                                  >  1:122716/1‑150 (MQ=255)

                                             gACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGc                                                                                                  >  2:317866/1‑150 (MQ=255)

                                              aCCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCAt                                                                                                >  2:694637/1‑151 (MQ=255)

                                               ccGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCAtt                                                                                               <  1:360039/151‑1 (MQ=255)

                                               ccGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCAtt                                                                                               >  4:246739/1‑151 (MQ=255)

                                                cGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTc                                                                                              >  2:394769/1‑151 (MQ=255)

                                                 gCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCg                                                                                             <  4:286200/151‑1 (MQ=255)

                                                  ccTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGc                                                                                            >  3:966788/1‑151 (MQ=255)

                                                      gCAACTCTGCCGACCTACGAAGAAGCAATTGCCCGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTTCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTAc                                                                                        <  3:323678/151‑1 (MQ=255)

                                                      gCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTAc                                                                                        <  1:94816/151‑1 (MQ=255)

                                                      gCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTAc                                                                                        <  1:165918/151‑1 (MQ=255)

                                                       cAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACg                                                                                       >  4:416493/1‑151 (MQ=255)

                                                        aaCTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGt                                                                                      >  3:136548/1‑151 (MQ=255)

                                                        aaCTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGt                                                                                      >  3:75428/1‑151 (MQ=255)

                                                            ctGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGtata                                                                                   >  2:696028/1‑150 (MQ=255)

                                                            ctGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCAAGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGtat                                                                                    <  4:388958/149‑1 (MQ=255)

                                                              gCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGtata                                                                                   <  2:1091302/148‑1 (MQ=255)

                                                                cGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACtt                                                                              <  2:444148/151‑1 (MQ=255)

                                                                cGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACtt                                                                              <  1:220487/151‑1 (MQ=255)

                                                                 gACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACtt                                                                              >  2:487640/1‑150 (MQ=255)

                                                                 gACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTa                                                                             <  3:922690/151‑1 (MQ=255)

                                                                  aCCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTAt                                                                            >  3:442336/1‑151 (MQ=255)

                                                                    cTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTc                                                                          >  3:279513/1‑151 (MQ=255)

                                                                      aCGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTc                                                                          <  2:695049/149‑1 (MQ=255)

                                                                       cGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGa                                                                       <  4:21093/151‑1 (MQ=255)

                                                                           gaagCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATAtt                                                                   <  1:94591/151‑1 (MQ=255)

                                                                            aagCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTc                                                                  >  2:602247/1‑151 (MQ=255)

                                                                             agCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAATCTTATTCTGATAtt                                                                   <  3:491497/149‑1 (MQ=255)

                                                                             agCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTc                                                                  >  4:351666/1‑150 (MQ=255)

                                                                               cAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAgg                                                               <  4:137586/151‑1 (MQ=255)

                                                                                     cACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAAt                                                         >  3:1211087/1‑151 (MQ=255)

                                                                                      aCGCTTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAAtt                                                        <  2:214949/151‑1 (MQ=255)

                                                                                       cGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAAtt                                                        <  3:1303430/150‑1 (MQ=255)

                                                                                         ccTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTa                                                      >  2:1070933/1‑150 (MQ=255)

                                                                                            gATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATg                                                  <  1:245744/151‑1 (MQ=255)

                                                                                            gATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATg                                                  >  1:75757/1‑151 (MQ=255)

                                                                                               ggCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCt                                               >  2:419996/1‑151 (MQ=255)

                                                                                                gCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCt                                               >  4:450776/1‑150 (MQ=255)

                                                                                                gCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCt                                               >  2:4469/1‑150 (MQ=255)

                                                                                                    ccATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCAc                                          <  2:562748/151‑1 (MQ=255)

                                                                                                        gAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTaaa                                      <  2:75418/151‑1 (MQ=255)

                                                                                                        gAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTaa                                       <  2:855660/150‑1 (MQ=255)

                                                                                                          aagaagCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGa                                    <  3:696052/151‑1 (MQ=255)

                                                                                                            gaagCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTaa                                       >  2:253281/1‑146 (MQ=255)

                                                                                                             aagCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATc                                  >  2:748941/1‑150 (MQ=255)

                                                                                                             aagCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCa                                 <  3:932273/151‑1 (MQ=255)

                                                                                                               gCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAATTGTCTATCACTAAAGATCaa                                >  3:1153223/1‑150 (MQ=255)

                                                                                                                   cGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCa                            <  1:161410/150‑1 (MQ=255)

                                                                                                                    ggCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCAtt                          <  1:381607/151‑1 (MQ=255)

                                                                                                                    ggCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCAtt                          <  2:446984/151‑1 (MQ=255)

                                                                                                                       tGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCATTGa                        >  3:496386/1‑150 (MQ=255)

                                                                                                                           aaaCTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCATTGAAGc                     >  3:250219/1‑149 (MQ=255)

                                                                                                                                 gTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCATTGAAGCAGTTGCAg             <  1:467243/151‑1 (MQ=255)

                                                                                                                                  ttCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCATTGAAGCAGTTGCAGc            >  2:1218166/1‑151 (MQ=255)

                                                                                                                                    cGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCATTGAAGCAGTTGCAGCt           >  3:571815/1‑150 (MQ=255)

                                                                                                                                    cGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCATTGAAGCAGTTGCAGCt           <  3:239762/150‑1 (MQ=255)

                                                                                                                                     gTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCATTGAAGCAGTTGCAGCTAt         >  2:1262354/1‑151 (MQ=255)

                                                                                                                                     gTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCATTGAAGCAGTTGCAGCTAt         <  1:364150/151‑1 (MQ=255)

                                                                                                                                       aCTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCATTGAAGCAGTTGCAGCTATg        <  2:715745/150‑1 (MQ=255)

                                                                                                                                       aCTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCATTGAAGCAGTTGCAGCTATGt       >  3:292724/1‑151 (MQ=255)

                                                                                                                                         tctGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCATTGAAGCAGTTGCAGCTATGTCt     >  3:268810/1‑151 (MQ=255)

                                                                                                                                         tctGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCATTGAAGCAGTTGCAGCTATGTCt     >  2:843609/1‑151 (MQ=255)

                                                                                                                                           tGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCATTGAAGCAGTTGCAGCTATGTCTGt   >  3:760750/1‑151 (MQ=255)

                                                                                                                                           tGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACT‑AAGATCAAATCATTGAAGCAGTTGCAGCTATGTCTGt   >  2:945516/1‑150 (MQ=255)

                                                                                                                                            ggCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCATTGAAGCAGTTGCAGCTATGTCt     <  3:988744/148‑1 (MQ=255)

                                                                                                                                            ggCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCATTGAAGCAGTTGCAGCTATGTCTGTa  <  4:222339/151‑1 (MQ=255)

AAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCATTGAAGCAGTTGCAGCTATGTCTGTA  >  NC_000913/4180320‑4180610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`