BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,351,421	C→T	G81D (GGC→GAC)	dcuS ←	sensory histidine kinase in two‑component regulatory system with DcuR, regulator of anaerobic fumarate respiration

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,351,421	0	C	T	100.0%	455.4 / NA	116	G81D (GGC→GAC)	dcuS	sensory histidine kinase in two‑component regulatory system with DcuR, regulator of anaerobic fumarate respiration
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (69/47); total (69/47)

Only 100 of 116 total aligned reads displayed.

AGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGC  >  NC_000913/4351277‑4351567

aGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCActct                                                                                                                                              >  1:73901/1‑151 (MQ=255)

aGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCActct                                                                                                                                              <  1:256277/151‑1 (MQ=255)

      ttAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTg                                                                                                                                          <  3:1315425/149‑1 (MQ=255)

        aaGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCgg                                                                                                                                       <  3:1058393/150‑1 (MQ=255)

        aaGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGt                                                                                                                                      >  2:862236/1‑151 (MQ=255)

         aGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGtt                                                                                                                                     >  3:1281428/1‑151 (MQ=255)

         aGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGtt                                                                                                                                     >  2:125333/1‑151 (MQ=255)

           gATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGtttt                                                                                                                                   <  2:1375108/151‑1 (MQ=255)

            aTGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGttttt                                                                                                                                  >  1:211292/1‑151 (MQ=255)

            aTGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGttttt                                                                                                                                  <  3:1005323/151‑1 (MQ=255)

              gTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGtttttt                                                                                                                                 <  1:247754/150‑1 (MQ=255)

                  tcaCCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGc                                                                                                                             >  3:1392824/1‑150 (MQ=255)

                   caCCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCa                                                                                                                            >  2:1074534/1‑150 (MQ=255)

                     ccTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGc                                                                                                                         <  1:290632/151‑1 (MQ=255)

                      cTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGcc                                                                                                                        >  4:245954/1‑151 (MQ=255)

                        ttAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGTGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGccc                                                                                                                       <  2:906379/150‑1 (MQ=255)

                         tAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCt                                                                                                                      <  1:109553/150‑1 (MQ=255)

                         tAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTg                                                                                                                     >  4:145434/1‑151 (MQ=255)

                               gCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGAt                                                                                                               <  2:146260/151‑1 (MQ=255)

                                 tGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGAttt                                                                                                             >  3:197182/1‑151 (MQ=255)

                                  gACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGAtt                                                                                                              <  2:40357/149‑1 (MQ=255)

                                     cAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCgg                                                                                                         >  2:1235312/1‑151 (MQ=255)

                                     cAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCgg                                                                                                         <  2:128626/151‑1 (MQ=255)

                                     cAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCgg                                                                                                         <  2:721532/151‑1 (MQ=255)

                                      aaTACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGc                                                                                                        >  4:218211/1‑151 (MQ=255)

                                      aaTACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGc                                                                                                        <  3:830976/151‑1 (MQ=255)

                                         aCGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCGAGCCCTGACGGATTTCCGGCg                                                                                                       >  4:133182/1‑149 (MQ=255)

                                         aCGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGa                                                                                                      <  2:696982/150‑1 (MQ=255)

                                          cGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGt                                                                                                    <  3:602086/151‑1 (MQ=255)

                                           gCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTc                                                                                                   >  2:228042/1‑151 (MQ=255)

                                            cTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCg                                                                                                  >  3:512619/1‑151 (MQ=255)

                                              gggCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCgg                                                                                                 <  4:58660/150‑1 (MQ=255)

                                              gggCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGc                                                                                                <  2:1238956/151‑1 (MQ=255)

                                                     aGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGt                                                                                          >  2:69309/1‑150 (MQ=255)

                                                     aGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGt                                                                                          >  1:459549/1‑150 (MQ=255)

                                                       gATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTAcg                                                                                       <  2:1281362/151‑1 (MQ=255)

                                                           cGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGcc                                                                                   >  2:1323956/1‑151 (MQ=255)

                                                            gAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGcc                                                                                   >  3:1154334/1‑150 (MQ=255)

                                                             aGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCAc                                                                                 >  3:211696/1‑151 (MQ=255)

                                                                   gAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGcc                                                                             >  3:1073234/1‑149 (MQ=255)

                                                                     aGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTg                                                                         >  3:660469/1‑151 (MQ=255)

                                                                     aGACTTTGCAGATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTg                                                                         >  2:1206992/1‑151 (MQ=255)

                                                                       aCTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGcc                                                                       >  3:1216755/1‑151 (MQ=255)

                                                                       aCTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGcc                                                                       <  2:361497/151‑1 (MQ=255)

                                                                        cTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCt                                                                      >  2:322584/1‑151 (MQ=255)

                                                                         tttGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCtt                                                                     >  2:118759/1‑151 (MQ=255)

                                                                            gCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCttgtt                                                                  >  3:141973/1‑151 (MQ=255)

                                                                             cATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCttgttg                                                                 >  2:843516/1‑151 (MQ=255)

                                                                              atatCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTgg                                                                >  3:548500/1‑151 (MQ=255)

                                                                              atatCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTgg                                                                >  1:75423/1‑151 (MQ=255)

                                                                                atCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCt                                                              >  3:1105577/1‑151 (MQ=255)

                                                                                 tCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGc                                                               >  2:937565/1‑149 (MQ=255)

                                                                                 tCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCt                                                              >  2:708030/1‑150 (MQ=255)

                                                                                  cGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTa                                                             <  3:840712/150‑1 (MQ=255)

                                                                                   ggTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTGCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGc                                                           <  2:1154277/151‑1 (MQ=255)

                                                                                     tAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGccc                                                         >  3:51348/1‑151 (MQ=255)

                                                                                     tAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGccc                                                         >  3:887460/1‑151 (MQ=255)

                                                                                       aCGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCAt                                                       >  3:689982/1‑151 (MQ=255)

                                                                                          aCAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCAtctc                                                    >  3:1292411/1‑151 (MQ=255)

                                                                                                 aCAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCat                                             >  3:597400/1‑151 (MQ=255)

                                                                                                 aCAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCat                                             <  1:409074/151‑1 (MQ=255)

                                                                                                 aCAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCat                                             >  3:756216/1‑151 (MQ=255)

                                                                                                 aCAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCat                                             <  3:730510/151‑1 (MQ=255)

                                                                                                  cagcagATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCata                                            <  1:111252/151‑1 (MQ=255)

                                                                                                  cagcagATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCata                                            <  2:404878/151‑1 (MQ=255)

                                                                                                   agcagATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCatat                                           >  3:737309/1‑151 (MQ=255)

                                                                                                   agcagATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCatat                                           >  2:1287153/1‑151 (MQ=255)

                                                                                                    gcagATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATc                                          >  3:579928/1‑151 (MQ=255)

                                                                                                     cagATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCa                                              <  1:113136/146‑1 (MQ=255)

                                                                                                     cagATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCa                                              >  4:113135/1‑146 (MQ=255)

                                                                                                     cagATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCa                                         <  3:1394123/151‑1 (MQ=255)

                                                                                                      agATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCAc                                        >  4:406276/1‑151 (MQ=255)

                                                                                                      agATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCAc                                        >  2:135541/1‑151 (MQ=255)

                                                                                                        aTCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTg                                      <  2:1302143/151‑1 (MQ=255)

                                                                                                          cGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCGTATCACTGa                                     <  2:1078618/150‑1 (MQ=255)

                                                                                                          cGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGa                                     <  3:974568/150‑1 (MQ=255)

                                                                                                          cGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGAt                                    >  2:1061958/1‑151 (MQ=255)

                                                                                                           gTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGAtt                                   >  2:1000743/1‑151 (MQ=255)

                                                                                                            ttgcgtttgcgtACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGAttt                                  >  2:416746/1‑151 (MQ=255)

                                                                                                               cgtttgcgtACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCg                               >  2:729723/1‑151 (MQ=255)

                                                                                                                   tgcgtACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCgaga                            >  3:981652/1‑150 (MQ=255)

                                                                                                                    gcgtACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGaa                           <  2:548468/150‑1 (MQ=255)

                                                                                                                     cgtACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGt                         <  2:2160/151‑1 (MQ=255)

                                                                                                                      gtACGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTa                        <  1:438936/151‑1 (MQ=255)

                                                                                                                        aCGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTaa                       <  2:456740/150‑1 (MQ=255)

                                                                                                                         cGGCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAAt                     <  3:485717/151‑1 (MQ=255)

                                                                                                                          ggCTTCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATc                    >  2:835082/1‑151 (MQ=255)

                                                                                                                             ttCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATc                    >  2:603024/1‑148 (MQ=255)

                                                                                                                              tCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAg                  <  2:660447/149‑1 (MQ=255)

                                                                                                                              tCCGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAg                  <  2:981611/149‑1 (MQ=255)

                                                                                                                               ccGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATg               >  3:785893/1‑151 (MQ=255)

                                                                                                                               ccGCGATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATg               <  1:245957/151‑1 (MQ=255)

                                                                                                                                cgcgATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGc              >  2:367780/1‑151 (MQ=255)

                                                                                                                                 gcgATGGCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGc              >  2:886518/1‑150 (MQ=255)

                                                                                                                                      ggCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCaccacc        <  2:689957/151‑1 (MQ=255)

                                                                                                                                      ggCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCaccacc        <  3:1235371/151‑1 (MQ=255)

                                                                                                                                       gCCTGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCAccacca       <  2:121606/151‑1 (MQ=255)

                                                                                                                                          tGGATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAAt     >  3:1026412/1‑150 (MQ=255)

                                                                                                                                           ggATGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAg   <  3:228068/151‑1 (MQ=255)

                                                                                                                                             aTGTCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGc  >  2:863990/1‑150 (MQ=255)

AGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGC  >  NC_000913/4351277‑4351567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`