Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,423,096 T→C L84P (CTG→CCG)  ulaE → L‑xylulose 5‑phosphate 3‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,423,0960TC99.1% 411.4 / NA 113L84P (CTG→CCG) ulaEL‑xylulose 5‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (49/63);  total (49/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 114 total aligned reads displayed.

TTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTC  >  NC_000913/4422948‑4423242
                                                                                                                                                    |                                                                                                                                                  
tttGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCgg                                                                                                                                                  >  3:40155/1‑151 (MQ=255)
 ttGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCgg                                                                                                                                                  <  3:422201/150‑1 (MQ=255)
   gTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGc                                                                                                                                                >  2:125551/1‑150 (MQ=255)
    tCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGc                                                                                                                                                >  3:661766/1‑149 (MQ=255)
     cGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGt                                                                                                                                             <  1:360071/151‑1 (MQ=255)
     cGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCATTTCTGCTCATCGTCGTTTCCCGCCGGGCAg                                                                                                                                              >  3:284008/1‑150 (MQ=255)
      gAAATGTCGGTAGATGAACCTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTg                                                                                                                                            <  3:120987/151‑1 (MQ=255)
      gAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGt                                                                                                                                             <  2:661744/150‑1 (MQ=255)
        aaTGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGa                                                                                                                                           >  2:759047/1‑150 (MQ=255)
         aTGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGa                                                                                                                                           >  1:272202/1‑149 (MQ=255)
            tCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATg                                                                                                                                      >  2:157625/1‑151 (MQ=255)
             cGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGCAGATg                                                                                                                                      <  1:165404/150‑1 (MQ=255)
             cGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATg                                                                                                                                      >  2:748700/1‑150 (MQ=255)
              ggTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGAc                                                                                                                                    <  2:651974/151‑1 (MQ=255)
               gTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGAcg                                                                                                                                   <  1:324244/151‑1 (MQ=255)
               gTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGAcg                                                                                                                                   >  3:766784/1‑151 (MQ=255)
                tAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGAcgc                                                                                                                                  >  3:337028/1‑151 (MQ=255)
                 aGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGAcgcg                                                                                                                                 <  2:511545/151‑1 (MQ=255)
                   aTGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCgg                                                                                                                                >  3:415715/1‑150 (MQ=255)
                    tGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTg                                                                                                                              >  3:254162/1‑151 (MQ=255)
                     gAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTg                                                                                                                              >  1:17621/1‑150 (MQ=255)
                      aaaCTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCg                                                                                                                            <  2:1181054/151‑1 (MQ=255)
                          tGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGgcg                                                                                                                        >  1:381750/1‑151 (MQ=255)
                           gACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGgc                                                                                                                         <  3:264455/149‑1 (MQ=255)
                            aCGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCa                                                                                                                      <  2:927775/151‑1 (MQ=255)
                             cGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTTCCGTCCATGCGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGgcgc                                                                                                                       >  2:540022/1‑149 (MQ=255)
                                 cGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAgggg                                                                                                                  <  3:193741/150‑1 (MQ=255)
                                   ccTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTg                                                                                                               >  4:390338/1‑151 (MQ=255)
                                   ccTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTg                                                                                                               <  2:751323/151‑1 (MQ=255)
                                   ccTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTg                                                                                                               <  3:1121452/151‑1 (MQ=255)
                                    cTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTgg                                                                                                              >  3:308550/1‑151 (MQ=255)
                                     tGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTgg                                                                                                              >  2:100009/1‑150 (MQ=255)
                                     tGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTgg                                                                                                              >  1:94629/1‑150 (MQ=255)
                                      gTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGgag                                                                                                            <  3:25586/151‑1 (MQ=255)
                                            ccTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATg                                                                                                      >  3:616594/1‑151 (MQ=255)
                                             cTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGc                                                                                                     >  2:137472/1‑151 (MQ=255)
                                               cAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGc                                                                                                     <  4:401917/149‑1 (MQ=255)
                                               cAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCg                                                                                                    <  2:1386413/150‑1 (MQ=255)
                                               cAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGt                                                                                                   <  1:455819/151‑1 (MQ=255)
                                                 aCTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTaa                                                                                                 <  3:494774/151‑1 (MQ=255)
                                                    ggAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGc                                                                                              <  2:605014/151‑1 (MQ=255)
                                                     gAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCt                                                                                             <  2:1230577/151‑1 (MQ=255)
                                                         cgcgAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTGTGCGTAAAGCTATcc                                                                                         >  3:983438/1‑151 (MQ=255)
                                                         cgcgAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATcc                                                                                         >  3:807325/1‑151 (MQ=255)
                                                         cgcgAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATcc                                                                                         <  3:1092468/151‑1 (MQ=255)
                                                         cgcgAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTGTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATcc                                                                                         <  2:159208/151‑1 (MQ=255)
                                                           cgAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATcc                                                                                         >  2:760464/1‑149 (MQ=255)
                                                            gAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGt                                                                                      >  4:290740/1‑151 (MQ=255)
                                                            gAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGt                                                                                      >  2:850535/1‑151 (MQ=255)
                                                              gcagcGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGtt                                                                                     <  3:1159254/150‑1 (MQ=255)
                                                               cagcGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCg                                                                                   >  4:274752/1‑151 (MQ=255)
                                                                   gTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCa                                                                               <  4:300803/151‑1 (MQ=255)
                                                                   gTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCa                                                                               <  2:674213/151‑1 (MQ=255)
                                                                   gTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCa                                                                               <  3:485649/151‑1 (MQ=255)
                                                                       ggCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAgg                                                                             <  2:1146898/149‑1 (MQ=255)
                                                                           cTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCg                                                                       >  3:944462/1‑151 (MQ=255)
                                                                            tGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTc                                                                        <  2:349334/149‑1 (MQ=255)
                                                                            tGGTCAATGCGATTGTTGAAACCGGCGGGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCTTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTGAAGCTATCCAGTTCGCCCAGGAttt                                                                         <  3:518332/148‑3 (MQ=255)
                                                                             ggTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCgg                                                                      <  2:727244/150‑1 (MQ=255)
                                                                             ggTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCgg                                                                      <  2:623014/150‑1 (MQ=255)
                                                                             ggTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGt                                                                     >  4:379556/1‑151 (MQ=255)
                                                                                      gATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTg                                                            <  2:803107/151‑1 (MQ=255)
                                                                                       aTTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTg                                                            >  4:223999/1‑150 (MQ=255)
                                                                                       aTTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTg                                                            >  2:1397882/1‑150 (MQ=255)
                                                                                        ttgttgAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGAt                                                          >  3:1263540/1‑151 (MQ=255)
                                                                                          gttgAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATc                                                         <  3:157645/150‑1 (MQ=255)
                                                                                          gttgAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAGCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATc                                                         <  3:1104148/150‑1 (MQ=255)
                                                                                           ttgAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATcc                                                        >  2:919181/1‑150 (MQ=255)
                                                                                            tgAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATcc                                                        >  3:1068646/1‑149 (MQ=255)
                                                                                               aaCCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTg                                                   >  1:199937/1‑151 (MQ=255)
                                                                                               aaCCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTg                                                   >  3:1010621/1‑151 (MQ=255)
                                                                                               aaCCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTg                                                   >  2:173253/1‑151 (MQ=255)
                                                                                                aCCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTgg                                                  <  2:807290/151‑1 (MQ=255)
                                                                                                  cGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCg                                                >  4:241769/1‑151 (MQ=255)
                                                                                                   ggCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCgg                                               >  4:358148/1‑151 (MQ=255)
                                                                                                      gtgcgcgtgcCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCt                                            <  2:262981/151‑1 (MQ=255)
                                                                                                        gcgcgtgcCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTa                                           <  3:137488/150‑1 (MQ=255)
                                                                                                        gcgcgtgcCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTAt                                          <  4:344842/151‑1 (MQ=255)
                                                                                                          gcgtgcCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGa                                        <  4:288410/151‑1 (MQ=255)
                                                                                                              gcCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGtt                                    <  2:440267/151‑1 (MQ=255)
                                                                                                                cGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTa                                  <  2:1219830/151‑1 (MQ=255)
                                                                                                                   ccATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACt                                <  2:283983/150‑1 (MQ=255)
                                                                                                                    cATGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTAt                              <  1:187938/151‑1 (MQ=255)
                                                                                                                     aTGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATc                             >  2:1327231/1‑151 (MQ=255)
                                                                                                                     aTGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATc                             >  1:367067/1‑151 (MQ=255)
                                                                                                                     aTGTGCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATc                             <  3:100023/151‑1 (MQ=255)
                                                                                                                      tgtgCCTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATc                             >  2:617350/1‑150 (MQ=255)
                                                                                                                          ccTTTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATACGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGa                         >  3:1250860/1‑150 (MQ=255)
                                                                                                                          ccTGTCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGaa                        <  4:387742/151‑1 (MQ=255)
                                                                                                                            tttCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGCGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGc                      >  3:483727/1‑151 (MQ=255)
                                                                                                                             ttCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAg                       <  2:1217804/149‑1 (MQ=255)
                                                                                                                             ttCTGCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGc                      <  1:405445/150‑1 (MQ=255)
                                                                                                                                 gCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGcct                    <  3:759081/148‑2 (MQ=255)
                                                                                                                                 gCTCATCGTCGTTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAAt                  <  4:403693/150‑1 (MQ=255)
                                                                                                                                       cgtcgtTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGaaa           <  2:415684/151‑1 (MQ=255)
                                                                                                                                        gtcgtTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGaaa           <  3:748732/150‑1 (MQ=255)
                                                                                                                                          cgtTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTGAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAAcgc        >  1:174655/1‑151 (MQ=255)
                                                                                                                                          cgtTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAAcgc        <  3:1263718/151‑1 (MQ=255)
                                                                                                                                          cgtTTCCCGCCGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAAcg         <  2:1202527/150‑1 (MQ=255)
                                                                                                                                                ccgccgGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGcgtcgtc  >  1:363883/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTC  >  NC_000913/4422948‑4423242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑