Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,431,246 T→C G461G (GGT→GGC)  cycA → D‑alanine/D‑serine/glycine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,431,2460TC100.0% 443.8 / NA 115G461G (GGT→GGC) cycAD‑alanine/D‑serine/glycine transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (51/64);  total (51/64)

Only 100 of 115 total aligned reads displayed.

CCGCTCGGCAAGCTGATGTGCTGGGTATGTATGGCGTTCTTTGTGTTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGTAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAAT  >  NC_000913/4431100‑4431392
                                                                                                                                                  |                                                                                                                                                  
ccGCTCGGCAAGCTGATGTGCTGGGTATGTATGGCGTTCTTTGTGTTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCaaga                                                                                                                                                <  2:196108/151‑1 (MQ=255)
ccGCTCGGCAAGCTGATGTGCTGGGTATGTATGGCGTTCTTTGTGTTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCaaga                                                                                                                                                <  2:38233/151‑1 (MQ=255)
 cGCTCGGCAAGCTGATGTGCTGGGTATGTATGGCGTTCTTTGTGTTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCaagaa                                                                                                                                               <  3:891989/151‑1 (MQ=255)
    tCGGCAAGCTGATGTGCTGGGTATGTATGGCGTTCTTTGTGTTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCg                                                                                                                                            >  1:141223/1‑151 (MQ=255)
     cGGCAAGCTGATGTGCTGGGTATGTATGGCGTTCTTTGTGTTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCgg                                                                                                                                           >  4:32771/1‑151 (MQ=255)
     cGGCAAGCTGATGTGCTGGGTATGTATGGCGTTCTTTGTGTTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCgg                                                                                                                                           >  2:1300646/1‑151 (MQ=255)
         aaGCTGATGTGCTGGGTATGTATGGCGTTCTTTGTGTTCGTGGTCGTTTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGgct                                                                                                                                        <  3:1010671/150‑1 (MQ=255)
          aGCTGATGTGCTGGGTATGTATGGCGTTCTTTGTGTTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGgctgc                                                                                                                                      >  2:161774/1‑151 (MQ=255)
          aGCTGATGTGCTGGGTATGTATGGCGTTCTTTGTGGTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGgctgc                                                                                                                                      >  4:407098/1‑151 (MQ=255)
           gCTGATGTGCTGGGTATGTATGGCGTTCTTTGTGTTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGgctgct                                                                                                                                     >  4:295029/1‑151 (MQ=255)
            cTGATGTGCTGGGTATGTATGGCGTTCTTTGTGTTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGctgctg                                                                                                                                    >  2:768987/1‑151 (MQ=255)
              gATGTGCTGGGTATGTATGGCGTTCTTTGTGTTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGctgctg                                                                                                                                    <  2:1148746/149‑1 (MQ=255)
               aTGTGCTGGGTATGTATGGCGTTCTTTGTGTTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAAc                                                                                                                                 <  1:13044/151‑1 (MQ=255)
                 gtgCTGGGTATGTATGGCGTTCTTTGTGTTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTg                                                                                                                               >  2:747300/1‑151 (MQ=255)
                     tGGGTATGTATGGCGTTCTTTGTGTTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCgg                                                                                                                            >  1:29614/1‑150 (MQ=255)
                      gggTATGTATGGCGTTCTTTGTGTTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGa                                                                                                                           <  4:272401/150‑1 (MQ=255)
                      gggTATGTATGGCGTTCTTTGTGTTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGa                                                                                                                           <  2:500649/150‑1 (MQ=255)
                          atgtatgGCGTTCTTTGTGTTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATa                                                                                                                       >  1:140271/1‑150 (MQ=255)
                           tgtatgGCGTTCTTTGTGTTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAAc                                                                                                                     >  3:800429/1‑151 (MQ=255)
                            gtatgGCGTTCTTTGTGTTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAAcc                                                                                                                    >  2:505460/1‑151 (MQ=255)
                            gtatgGCGTTCTTTGTGTTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAAcc                                                                                                                    >  2:1005215/1‑151 (MQ=255)
                             tatgGCGTTCTTTGTGTTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCg                                                                                                                   >  2:121812/1‑151 (MQ=255)
                             tatgGCGTTCTTTGTGTTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCg                                                                                                                   >  2:647560/1‑151 (MQ=255)
                              atgGCGTTCTTTGTGTTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCg                                                                                                                   <  2:264505/150‑1 (MQ=255)
                              atgGCGTTCTTTGTGTTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGc                                                                                                                  <  2:577237/151‑1 (MQ=255)
                               tgGCGTTCTTTGTGTTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGc                                                                                                                  >  3:64726/1‑150 (MQ=255)
                                ggCGTTCTTTGTGTTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCAt                                                                                                                <  4:65506/151‑1 (MQ=255)
                                ggCGTTCTTTGTGTTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCAt                                                                                                                >  2:1081492/1‑151 (MQ=255)
                                   gTTCTTTGTGTTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTAt                                                                                                             <  3:646650/151‑1 (MQ=255)
                                        ttGTGTTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATg                                                                                                         <  2:1311319/150‑1 (MQ=255)
                                        ttGTGTTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATg                                                                                                         <  2:132184/150‑1 (MQ=255)
                                        ttGTGTTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGc                                                                                                        >  4:170536/1‑151 (MQ=255)
                                          gtgtTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCt                                                                                                       <  3:157107/150‑1 (MQ=255)
                                          gtgtTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTg                                                                                                      <  2:364270/151‑1 (MQ=255)
                                          gtgtTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTg                                                                                                      <  2:1341176/151‑1 (MQ=255)
                                          gtgtTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTg                                                                                                      >  3:1181747/1‑151 (MQ=255)
                                           tgtTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTgg                                                                                                     >  4:429296/1‑151 (MQ=255)
                                            gtTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGa                                                                                                    <  1:271678/151‑1 (MQ=255)
                                              tCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGAt                                                                                                   <  2:486269/150‑1 (MQ=255)
                                              tCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATg                                                                                                  >  2:1105671/1‑151 (MQ=255)
                                               cGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATgg                                                                                                 <  1:388124/151‑1 (MQ=255)
                                                gTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGgc                                                                                                <  3:466125/151‑1 (MQ=255)
                                                gTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGgc                                                                                                >  2:720463/1‑151 (MQ=255)
                                                   gTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGgcgc                                                                                              >  3:953466/1‑150 (MQ=255)
                                                    tCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCa                                                                                             >  1:225453/1‑150 (MQ=255)
                                                        gtTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGcc                                                                                        <  4:472108/151‑1 (MQ=255)
                                                        gtTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGcc                                                                                        <  2:1058268/151‑1 (MQ=255)
                                                         ttGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGGTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGcc                                                                                        >  4:256857/1‑150 (MQ=255)
                                                          tGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCAt                                                                                      >  2:558033/1‑151 (MQ=255)
                                                           gCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATc                                                                                     <  1:154807/151‑1 (MQ=255)
                                                              gACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCa                                                                                   <  3:1197233/150‑1 (MQ=255)
                                                                cacTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTTTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCt                                                                                <  3:146015/151‑1 (MQ=255)
                                                                 acTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCtt                                                                               <  3:554335/151‑1 (MQ=255)
                                                                    ggAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCtttt                                                                             <  2:503853/150‑1 (MQ=255)
                                                                    ggAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCtttt                                                                             <  4:304137/150‑1 (MQ=255)
                                                                      aaGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGa                                                                          <  4:295814/151‑1 (MQ=255)
                                                                         aTGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGAtc                                                                        >  3:261750/1‑150 (MQ=255)
                                                                         aTGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCAGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGAtca                                                                       <  3:262764/151‑1 (MQ=255)
                                                                             cacTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGAtcactca                                                                   <  1:114349/151‑1 (MQ=255)
                                                                             cacTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGAtcactc                                                                    >  1:101948/1‑150 (MQ=255)
                                                                                 cGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCg                                                               <  2:1351679/151‑1 (MQ=255)
                                                                                  gCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGc                                                              >  3:1346112/1‑151 (MQ=255)
                                                                                    cAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGcc                                                             >  3:950287/1‑150 (MQ=255)
                                                                                    cAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCa                                                            <  3:1263992/151‑1 (MQ=255)
                                                                                     aGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGcc                                                             >  2:342879/1‑149 (MQ=255)
                                                                                       gcgcTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAgcg                                                         <  1:123820/151‑1 (MQ=255)
                                                                                       gcgcTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAgcg                                                         >  2:399676/1‑151 (MQ=255)
                                                                                         gctgctGGTTCCCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAgcgc                                                        <  2:770218/150‑1 (MQ=255)
                                                                                         gctgctGGTTACCCCCCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCGGGATGGCGCAATCCCATCCAGTTTTTAGATCCCTCACCCGCCAgcg                                                         <  3:679704/149‑1 (MQ=255)
                                                                                          ctgctgGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCCCCCGCCAgcgc                                                        <  3:747067/149‑1 (MQ=255)
                                                                                           tgctgGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGcgcgcg                                                     >  3:1204585/1‑151 (MQ=255)
                                                                                            gctgGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGt                                                    >  2:307336/1‑151 (MQ=255)
                                                                                            gctgGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTAACCTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAgcgcgc                                                      <  3:1392812/149‑1 (MQ=255)
                                                                                             ctgGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTg                                                   >  4:310713/1‑151 (MQ=255)
                                                                                                   aCCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAAc                                              >  4:478195/1‑150 (MQ=255)
                                                                                                   aCCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAAc                                              >  1:129761/1‑150 (MQ=255)
                                                                                                    ccccGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAg                                            <  3:403368/151‑1 (MQ=255)
                                                                                                       cGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTAc                                         >  3:565116/1‑151 (MQ=255)
                                                                                                       cGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTAc                                         <  2:848405/151‑1 (MQ=255)
                                                                                                                ttATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGtttt                                 <  1:371856/150‑1 (MQ=255)
                                                                                                                  aTCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTcc                               <  4:244791/150‑1 (MQ=255)
                                                                                                                  aTCGCGCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCa                              <  2:410124/151‑1 (MQ=255)
                                                                                                                    cgcgCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTcc                               >  3:1344103/1‑148 (MQ=255)
                                                                                                                    cgcgCTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGa                            <  2:250612/151‑1 (MQ=255)
                                                                                                                     gcgcTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCa                              <  3:828236/148‑1 (MQ=255)
                                                                                                                     gcgcTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGa                            <  2:210686/150‑1 (MQ=255)
                                                                                                                      cgcTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGGTGAACTGCGGAAATAACCGCATTATTATGCTGGATGGCGCAATGCCATCCTGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGa                            <  1:215188/149‑1 (MQ=255)
                                                                                                                      cgcTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGGGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGAc                           <  4:28274/150‑1 (MQ=255)
                                                                                                                       gcTGGGGTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGAc                           <  3:161796/149‑1 (MQ=255)
                                                                                                                           gggTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGAtgt                     <  2:565085/151‑1 (MQ=255)
                                                                                                                            ggTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGAtgtg                    >  3:960633/1‑151 (MQ=255)
                                                                                                                            ggTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGAtgtg                    >  2:418639/1‑151 (MQ=255)
                                                                                                                            ggTTGGGCTGGCTGTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCGGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGAtgtg                    <  4:200852/151‑1 (MQ=255)
                                                                                                                                    tggctgTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATc             >  2:1729/1‑150 (MQ=255)
                                                                                                                                     ggctgTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCCCCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAg           <  1:338977/151‑1 (MQ=255)
                                                                                                                                     ggctgTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATc             <  1:218788/149‑1 (MQ=255)
                                                                                                                                     ggctgTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATc             >  4:463960/1‑149 (MQ=255)
                                                                                                                                       ctgTTTATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAgg          >  3:803289/1‑150 (MQ=255)
                                                                                                                                           ttATTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGtcatc     >  3:290467/1‑151 (MQ=255)
                                                                                                                                             aTTGGCAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGGGCAATGCCATCCAGTTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGtcatca    <  3:178599/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CCGCTCGGCAAGCTGATGTGCTGGGTATGTATGGCGTTCTTTGTGTTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGCTGGTTACCCCGCTGTGGTTTATCGCGCTGGGGTTGGGCTGGCTGTTTATTGGTAAGAAGCGGGCTGCTGAACTGCGGAAATAACCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAAT  >  NC_000913/4431100‑4431392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑