BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,632,098	T→C	W456R (TGG→CGG)	slt →	lytic murein transglycosylase, soluble

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,632,098	0	T	C	100.0%	392.0 / NA	102	W456R (TGG→CGG)	slt	lytic murein transglycosylase, soluble
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (53/49); total (53/49)

Only 100 of 104 total aligned reads displayed.

GACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCA  >  NC_000913/4631950‑4632243

gACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAAtggcgg                                                                                                                                                 >  2:149180/1‑151 (MQ=255)

gACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAAtggcgg                                                                                                                                                 <  2:767488/151‑1 (MQ=255)

    cAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGa                                                                                                                                               <  2:1030929/149‑1 (MQ=255)

    cAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGAt                                                                                                                                              <  2:361340/150‑1 (MQ=255)

           cGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGt                                                                                                                                      <  1:111803/151‑1 (MQ=255)

            ggAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGtt                                                                                                                                     >  2:424746/1‑151 (MQ=255)

            ggAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGtt                                                                                                                                     >  2:630528/1‑151 (MQ=255)

               gaTGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTc                                                                                                                                    >  4:137240/1‑149 (MQ=255)

                aTGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGGTAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGGCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCACCAACCAATGGCGGGATCTTAGCGTTCa                                                                                                                                   <  2:729892/149‑1 (MQ=255)

                aTGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAgg                                                                                                                                 >  1:35785/1‑151 (MQ=255)

                aTGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAgg                                                                                                                                 >  1:68854/1‑151 (MQ=255)

                 tGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGc                                                                                                                                >  1:368650/1‑151 (MQ=255)

                  ggCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCTATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCa                                                                                                                               <  3:585314/151‑1 (MQ=255)

                  ggCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCa                                                                                                                               <  3:1314835/151‑1 (MQ=255)

                      cgcgTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACg                                                                                                                            >  3:520654/1‑150 (MQ=255)

                           gcgcGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGcc                                                                                                                      >  3:832398/1‑151 (MQ=255)

                           gcgcGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGcc                                                                                                                      <  3:187382/151‑1 (MQ=255)

                           gcgcGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGc                                                                                                                       <  4:284636/150‑1 (MQ=255)

                            cgcgAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCg                                                                                                                     <  2:888216/151‑1 (MQ=255)

                             gcgAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCgg                                                                                                                    <  3:1226779/151‑1 (MQ=255)

                             gcgAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCgg                                                                                                                    >  2:1251035/1‑151 (MQ=255)

                              cgAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCggg                                                                                                                   >  2:253971/1‑151 (MQ=255)

                              cgAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCggg                                                                                                                   >  1:408426/1‑151 (MQ=255)

                              cgAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCggg                                                                                                                   <  2:1165214/151‑1 (MQ=255)

                               gAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCggg                                                                                                                   >  3:785965/1‑150 (MQ=255)

                               gAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCggg                                                                                                                   >  3:967556/1‑150 (MQ=255)

                                            ggAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGatc                                                                                                      <  2:297878/150‑1 (MQ=255)

                                            ggAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGatc                                                                                                      <  4:260620/150‑1 (MQ=255)

                                                tctcGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCTGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCt                                                                                                  >  2:869354/1‑150 (MQ=255)

                                                 ctcGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTgg                                                                                                >  3:1404579/1‑151 (MQ=255)

                                                   cGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGatcatat                                                                                                  <  3:1238088/147‑3 (MQ=255)

                                                   cGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGaa                                                                                              <  3:228424/151‑1 (MQ=255)

                                                    gATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAag                                                                                             <  4:453742/151‑1 (MQ=255)

                                                     ataataCCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAag                                                                                             <  3:35041/150‑1 (MQ=255)

                                                      taataCCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAag                                                                                             >  1:137493/1‑149 (MQ=255)

                                                       aataCCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGGGGGATCATCTGGa                                                                                               >  2:1064133/1‑146 (MQ=255)

                                                        ataCCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCg                                                                                         >  3:201582/1‑151 (MQ=255)

                                                        ataCCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCg                                                                                         >  2:503261/1‑151 (MQ=255)

                                                         taCCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCg                                                                                         >  4:279905/1‑150 (MQ=255)

                                                          aCCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGAt                                                                                       <  2:948187/151‑1 (MQ=255)

                                                          aCCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGAt                                                                                       <  2:37963/151‑1 (MQ=255)

                                                           ccGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGAt                                                                                       <  2:642826/150‑1 (MQ=255)

                                                            cgcgcgTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTc                                                                                     <  2:1367732/151‑1 (MQ=255)

                                                                  tAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTg                                                                               >  2:33103/1‑151 (MQ=255)

                                                                   aGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTg                                                                               <  3:1009198/150‑1 (MQ=255)

                                                                      gAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCtt                                                                           <  3:167843/151‑1 (MQ=255)

                                                                           ggCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAAc                                                                      >  3:405096/1‑151 (MQ=255)

                                                                           ggCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAAc                                                                      >  2:495781/1‑151 (MQ=255)

                                                                              cAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGa                                                                    >  2:512152/1‑150 (MQ=255)

                                                                               aaTCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATc                                                                  >  3:386828/1‑151 (MQ=255)

                                                                               aaTCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATc                                                                  <  3:27706/151‑1 (MQ=255)

                                                                               aaTCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATc                                                                  <  2:239650/151‑1 (MQ=255)

                                                                                aTCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCt                                                                 >  3:949033/1‑151 (MQ=255)

                                                                                aTCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCt                                                                 >  4:435930/1‑151 (MQ=255)

                                                                                aTCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCt                                                                 >  2:277802/1‑151 (MQ=255)

                                                                                  cTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCttt                                                               >  4:403835/1‑151 (MQ=255)

                                                                                    ggTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCtt                                                                <  3:149197/148‑1 (MQ=255)

                                                                                    ggTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCtt                                                                <  4:150433/148‑1 (MQ=255)

                                                                                    ggTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTc                                                             >  3:1079522/1‑151 (MQ=255)

                                                                                    ggTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTc                                                             <  3:868798/151‑1 (MQ=255)

                                                                                     gTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCtt                                                                <  2:1082151/147‑1 (MQ=255)

                                                                                       gAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCaaa                                                          <  4:408422/151‑1 (MQ=255)

                                                                                            gCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCt                                                      >  1:80534/1‑150 (MQ=255)

                                                                                            gCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTa                                                     <  3:171749/151‑1 (MQ=255)

                                                                                              aaGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTaca                                                   <  1:51844/151‑1 (MQ=255)

                                                                                               aGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTacac                                                  <  4:444121/151‑1 (MQ=255)

                                                                                                  cAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAg                                               <  2:735706/151‑1 (MQ=255)

                                                                                                   aaaaaCAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGc                                              <  1:366864/151‑1 (MQ=255)

                                                                                                     aaaCAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCgg                                            >  4:161709/1‑151 (MQ=255)

                                                                                                       aCAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGt                                           >  2:313900/1‑150 (MQ=255)

                                                                                                         tgagCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTa                                          >  4:436836/2‑149 (MQ=255)

                                                                                                           agCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGga                                      <  2:1168862/151‑1 (MQ=255)

                                                                                                            gCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGgag                                     <  3:1064179/151‑1 (MQ=255)

                                                                                                             cAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAgg                                       >  2:1361055/1‑148 (MQ=255)

                                                                                                             cAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGgaga                                    <  2:1007452/151‑1 (MQ=255)

                                                                                                              aGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGAt                                   <  1:44580/151‑1 (MQ=255)

                                                                                                                 cTCAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATccc                                >  4:108234/1‑151 (MQ=255)

                                                                                                                   cAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCg                               <  3:277829/150‑1 (MQ=255)

                                                                                                                   cAACTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCg                               <  2:832362/150‑1 (MQ=255)

                                                                                                                    aaCTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCa                             <  4:259365/151‑1 (MQ=255)

                                                                                                                     aCTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGGAAGGAGATCCCGCa                             >  3:84523/1‑150 (MQ=255)

                                                                                                                     aCTGGCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAAAGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCa                             >  4:461809/1‑150 (MQ=255)

                                                                                                                        ggCGCGGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCaaa                           <  4:373991/149‑1 (MQ=255)

                                                                                                                          cgcgGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCt                        >  3:375922/1‑150 (MQ=255)

                                                                                                                           gcgGTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTa                       <  4:336895/150‑1 (MQ=255)

                                                                                                                            aggTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATg                     >  3:364304/2‑151 (MQ=255)

                                                                                                                             ggTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGc                    <  2:201553/151‑1 (MQ=255)

                                                                                                                             ggTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGc                    >  1:438470/1‑151 (MQ=255)

                                                                                                                              gTATGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCg                   >  3:383553/1‑151 (MQ=255)

                                                                                                                                aTGCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGAt                 >  1:177670/1‑151 (MQ=255)

                                                                                                                                  gCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATgg               >  1:43854/1‑151 (MQ=255)

                                                                                                                                  gCTTTCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATgg               >  2:603359/1‑151 (MQ=255)

                                                                                                                                     ttCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCg             <  3:152925/150‑1 (MQ=255)

                                                                                                                                      tCAACAACCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGa            >  2:929424/1‑150 (MQ=255)

                                                                                                                                        aacaacCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTg         >  2:1176671/1‑151 (MQ=255)

                                                                                                                                         acaacCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGc        >  2:11921/1‑151 (MQ=255)

                                                                                                                                         acaacCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGc        >  1:319917/1‑151 (MQ=255)

                                                                                                                                           aacCAATGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTc      >  3:1201146/1‑151 (MQ=255)

                                                                                                                                             ccAATGGCGGGATCTTAGCGTGCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGt    <  3:802672/151‑1 (MQ=255)

                                                                                                                                               aaTGGCGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCa  <  2:457086/151‑1 (MQ=255)

GACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAATGGTGGGATCTTAGCGTTCAGGCAACGATCGCCGGGAAGCTGTGGGATCATCTGGAAGAGCGATTCCCGCTGGCTTACAACGATCTTTTCAAACGCTACACCAGCGGTAAGGAGATCCCGCAAAGCTATGCGATGGCGATTGCTCGTCA  >  NC_000913/4631950‑4632243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`