BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	429,170	G→A	A508A (GCG→GCA)	secD →	SecYEG protein translocase auxillary subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	429,170	0	G	A	100.0%	482.9 / NA	118	A508A (GCG→GCA)	secD	SecYEG protein translocase auxillary subunit
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (49/69); total (49/69)

Only 100 of 123 total aligned reads displayed.

CCGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGGCGCATTCAGTTCTATCTTCGATGCGAAC  >  NC_000913/429022‑429317

ccGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCagg                                                                                                                                                   >  3:677794/1‑151 (MQ=255)

ccGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCagg                                                                                                                                                   >  3:50303/1‑151 (MQ=255)

 cGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGt                                                                                                                                                  >  4:126026/1‑151 (MQ=255)

 cGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGt                                                                                                                                                  <  1:463583/151‑1 (MQ=255)

 cGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGt                                                                                                                                                  >  2:202299/1‑151 (MQ=255)

   gTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTAt                                                                                                                                                <  1:223464/151‑1 (MQ=255)

     ctgctgGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCcaggtatcgcaggtatcg                                                                                                                                              >  3:945512/1‑151 (MQ=255)

       gctgGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGt                                                                                                                                             <  1:13830/150‑1 (MQ=255)

          ggTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCtt                                                                                                                                          <  2:816542/150‑1 (MQ=255)

           gTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTa                                                                                                                                         >  1:71194/1‑150 (MQ=255)

             ttCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCATGTATCGTCTTAAcc                                                                                                                                      >  1:68701/1‑151 (MQ=255)

                tATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCtt                                                                                                                                   >  2:939708/1‑151 (MQ=255)

                 aTTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCtt                                                                                                                                   <  2:1348922/150‑1 (MQ=255)

                 aTTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCtt                                                                                                                                   <  1:50905/150‑1 (MQ=255)

                   tCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCg                                                                                                                                >  1:247069/1‑151 (MQ=255)

                   tCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCg                                                                                                                                >  4:336831/1‑151 (MQ=255)

                   tCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCg                                                                                                                                >  1:81773/1‑151 (MQ=255)

                         cATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCg                                                                                                                          >  2:1356718/1‑151 (MQ=255)

                          aTGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCgg                                                                                                                         >  1:138404/1‑151 (MQ=255)

                          aTGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCgg                                                                                                                         <  2:204842/151‑1 (MQ=255)

                            gATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTc                                                                                                                       <  2:134511/151‑1 (MQ=255)

                            gATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTc                                                                                                                       <  4:138403/151‑1 (MQ=255)

                            gATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTc                                                                                                                       <  4:253179/151‑1 (MQ=255)

                               catcTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGa                                                                                                                     <  2:658102/150‑1 (MQ=255)

                                atcTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATg                                                                                                                   >  3:790847/1‑151 (MQ=255)

                                   ttctATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCg                                                                                                                 >  2:774776/1‑150 (MQ=255)

                                    tctATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCg                                                                                                                 <  1:365787/149‑1 (MQ=255)

                                    tctATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCg                                                                                                                 >  3:725485/1‑149 (MQ=255)

                                    tctATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGa                                                                                                                >  2:1136765/1‑150 (MQ=255)

                                     ctATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAAc                                                                                                              >  1:39107/1‑151 (MQ=255)

                                       ataAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGaa                                                                                                               <  2:808343/148‑1 (MQ=255)

                                       ataAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGt                                                                                                            <  1:448853/151‑1 (MQ=255)

                                          agaagTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACt                                                                                                         <  2:514214/151‑1 (MQ=255)

                                          agaagTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACt                                                                                                         <  2:790813/151‑1 (MQ=255)

                                            aagTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGa                                                                                                       <  2:673871/151‑1 (MQ=255)

                                             agTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGAt                                                                                                      <  1:134094/151‑1 (MQ=255)

                                                ttGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCa                                                                                                    <  2:691621/150‑1 (MQ=255)

                                                ttGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCa                                                                                                    >  3:1270496/1‑150 (MQ=255)

                                                     cTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCaacgaac                                                                                              >  2:1095644/1‑151 (MQ=255)

                                                     cTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCaacgaac                                                                                              >  3:11260/1‑151 (MQ=255)

                                                       gATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGt                                                                                            <  1:418919/151‑1 (MQ=255)

                                                         ttGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTAt                                                                                          >  3:405220/1‑151 (MQ=255)

                                                           gCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTAtt                                                                                         >  1:104969/1‑150 (MQ=255)

                                                             gACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTaaa                                                                                      <  4:339975/151‑1 (MQ=255)

                                                             gACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTaaa                                                                                      <  2:131662/151‑1 (MQ=255)

                                                                cAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAaagaa                                                                                   <  4:409386/151‑1 (MQ=255)

                                                                    gCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGACGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGt                                                                               >  3:102087/1‑151 (MQ=255)

                                                                         gATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTg                                                                             <  3:456805/148‑1 (MQ=255)

                                                                         gATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGc                                                                          <  3:1398672/151‑1 (MQ=255)

                                                                          aTTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGc                                                                          >  3:220349/1‑150 (MQ=255)

                                                                          aTTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCa                                                                         <  2:653316/151‑1 (MQ=255)

                                                                          aTTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCa                                                                         <  2:1171407/151‑1 (MQ=255)

                                                                             gCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAAc                                                                       <  2:377121/150‑1 (MQ=255)

                                                                             gCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACg                                                                      <  1:368359/151‑1 (MQ=255)

                                                                             gCCAACTTGATCTTAATCGTCGGAATTATGGCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTATCCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACg                                                                      <  3:1359121/151‑1 (MQ=255)

                                                                               cAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGa                                                                    <  2:677771/151‑1 (MQ=255)

                                                                               cAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGa                                                                    <  2:705453/151‑1 (MQ=255)

                                                                                aaCTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGAc                                                                   <  3:1095692/151‑1 (MQ=255)

                                                                                    tGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGt                                                                 <  2:254447/149‑1 (MQ=255)

                                                                                    tGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTa                                                                <  2:908165/150‑1 (MQ=255)

                                                                                       tCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGt                                                            >  2:364704/1‑151 (MQ=255)

                                                                                          tAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTTCTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGtt                                                           <  3:1199577/149‑1 (MQ=255)

                                                                                           aaTCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCaa                                                        <  1:435363/151‑1 (MQ=255)

                                                                                           aaTCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCa                                                         >  1:331474/1‑150 (MQ=255)

                                                                                            aTCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAAc                                                       <  2:1263299/151‑1 (MQ=255)

                                                                                              cgtcgGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACa                                                      <  1:181357/150‑1 (MQ=255)

                                                                                                tcgGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGc                                                   >  3:94286/1‑151 (MQ=255)

                                                                                                 cgGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGc                                                   >  4:106553/1‑150 (MQ=255)

                                                                                                  ggCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCaa                                                 <  3:1011719/151‑1 (MQ=255)

                                                                                                  ggCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCaa                                                 <  2:945475/151‑1 (MQ=255)

                                                                                                   gCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAAt                                                >  1:364300/1‑151 (MQ=255)

                                                                                                   gCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAAt                                                >  2:307828/1‑151 (MQ=255)

                                                                                                     aTTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAAtt                                               <  2:1282868/150‑1 (MQ=255)

                                                                                                     aTTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATtg                                              >  1:251854/1‑151 (MQ=255)

                                                                                                       tATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATtga                                             <  2:1261018/150‑1 (MQ=255)

                                                                                                          gTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGaa                                         <  2:1270430/151‑1 (MQ=255)

                                                                                                             gCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGt                                      >  1:199464/1‑151 (MQ=255)

                                                                                                              cTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGtt                                     >  4:426648/1‑151 (MQ=255)

                                                                                                               tGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGtt                                     >  3:1358273/1‑150 (MQ=255)

                                                                                                               tGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGtt                                     >  2:343395/1‑150 (MQ=255)

                                                                                                                gTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTAt                                   <  1:402054/151‑1 (MQ=255)

                                                                                                                   gCCAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGt                                <  1:114041/151‑1 (MQ=255)

                                                                                                                     cAGGCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTgg                              >  3:322453/1‑151 (MQ=255)

                                                                                                                       ggCGCAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGgcg                            <  3:1016149/151‑1 (MQ=255)

                                                                                                                         cgcAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGgcgc                           >  3:1038204/1‑150 (MQ=255)

                                                                                                                         cgcAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGgcgc                           >  3:519383/1‑150 (MQ=255)

                                                                                                                         cgcAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGGCGCa                          <  3:1356789/151‑1 (MQ=255)

                                                                                                                           cAACGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGGCGCAtt                        <  2:699300/151‑1 (MQ=255)

                                                                                                                             aCGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGGCGCATTCa                      <  3:316860/151‑1 (MQ=255)

                                                                                                                              cGCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGGCGCATTCAg                     <  2:405189/151‑1 (MQ=255)

                                                                                                                               gCTGAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGGCGCATTCAGt                    <  2:1213618/151‑1 (MQ=255)

                                                                                                                                  gAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGGCGCATTCAGtt                   <  2:59169/149‑1 (MQ=255)

                                                                                                                                  gAGTATGCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCAGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGGCGCATTCAGTTc                  <  2:1076380/150‑1 (MQ=255)

                                                                                                                                        gCCAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGGCGCATTCAGTTCTATCtt            <  2:725455/150‑1 (MQ=255)

                                                                                                                                         ccAGGTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGGCGCATTCAGTTCTATCTTCg          <  1:223874/151‑1 (MQ=255)

                                                                                                                                            ggTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGGCGCATTCAGTTCTATCTTCGATg       <  3:939746/151‑1 (MQ=255)

                                                                                                                                            ggTATCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGGCGCATTCAGTTCTATCTTCGATg       <  2:750650/151‑1 (MQ=255)

                                                                                                                                               aTCGCAGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGGCGCATTCAGTTCTATCTTCGATGCg     <  1:353407/150‑1 (MQ=255)

                                                                                                                                                 cgcaGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGGCGCATTCAGTTCTATCTTCGATGCGaa   <  4:255909/150‑1 (MQ=255)

                                                                                                                                                 cgcaGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGGCGCATTCAGTTCTATCTTCGATGCGAAc  >  1:230230/1‑151 (MQ=255)

CCGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGGCGCATTCAGTTCTATCTTCGATGCGAAC  >  NC_000913/429022‑429317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`