Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 487,092 T→C L186P (CTG→CCG)  mscK → mechanosensitive channel protein, intermediate conductance, K+ regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913487,0920TC100.0% 439.6 / NA 117L186P (CTG→CCG) mscKmechanosensitive channel protein, intermediate conductance, K+ regulated
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (59/58);  total (59/58)

Only 100 of 117 total aligned reads displayed.

GTTGCCCAGGCGCTGGACGATTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCTGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGC  >  NC_000913/486944‑487238
                                                                                                                                                    |                                                                                                                                                  
gTTGCCCAGGCGCTGGACGATTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCgg                                                                                                                                                  <  3:102051/151‑1 (MQ=255)
   gCCCAGGCGCTGGACGATTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATg                                                                                                                                               <  2:256257/151‑1 (MQ=255)
       gggCGCTGGACGATTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGAAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGAc                                                                                                                                           >  4:30178/2‑151 (MQ=255)
         gcgcTGGACGATTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTg                                                                                                                                         >  4:469331/1‑151 (MQ=255)
          cgcTGGACGATTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTg                                                                                                                                         >  1:375837/1‑150 (MQ=255)
              tgACGATTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTc                                                                                                                                    >  2:297825/2‑151 (MQ=255)
              ggACGATTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTc                                                                                                                                    >  1:200497/1‑151 (MQ=255)
                 cGATTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGc                                                                                                                                 <  2:1007029/151‑1 (MQ=255)
                  gATTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCg                                                                                                                                <  3:249147/151‑1 (MQ=255)
                  gATTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCg                                                                                                                                <  4:300947/151‑1 (MQ=255)
                   aTTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCga                                                                                                                               <  1:428241/151‑1 (MQ=255)
                   aTTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCg                                                                                                                                >  3:816670/1‑150 (MQ=255)
                   aTTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGAAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCg                                                                                                                                >  3:570759/1‑150 (MQ=255)
                        cAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGAc                                                                                                                            >  3:1256835/1‑149 (MQ=255)
                         aaaaCGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGc                                                                                                                         >  1:261660/1‑151 (MQ=255)
                           aaCGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCt                                                                                                                       >  2:62878/1‑151 (MQ=255)
                              gCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTAc                                                                                                                    <  3:575137/151‑1 (MQ=255)
                              gCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTAc                                                                                                                    >  4:158727/1‑151 (MQ=255)
                                acaAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACg                                                                                                                   >  3:815778/1‑150 (MQ=255)
                                acaAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGt                                                                                                                  <  2:1063388/151‑1 (MQ=255)
                                  aaaaCGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTcc                                                                                                                <  3:207214/151‑1 (MQ=255)
                                    aaCGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTccc                                                                                                               >  1:351860/1‑150 (MQ=255)
                                     aCGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTccc                                                                                                               >  2:1403601/1‑149 (MQ=255)
                                      cGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCccag                                                                                                             <  2:1102241/150‑1 (MQ=255)
                                          cTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCccagcc                                                                                                           >  3:318080/1‑148 (MQ=255)
                                          cTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCccagcc                                                                                                           >  2:557143/1‑148 (MQ=255)
                                           tGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGa                                                                                                        >  2:785963/1‑150 (MQ=255)
                                            ggCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCccagccag                                                                                                         <  2:1158872/148‑1 (MQ=255)
                                            ggCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCccagc                                                                                                            <  3:437810/145‑1 (MQ=255)
                                            ggCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGaa                                                                                                       <  2:1235972/150‑1 (MQ=255)
                                            ggCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGaa                                                                                                       >  3:1192623/1‑150 (MQ=255)
                                            ggCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGa                                                                                                        <  3:77613/149‑1 (MQ=255)
                                              cGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAgt                                                                                                    <  1:298261/151‑1 (MQ=255)
                                               gTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAgtg                                                                                                   <  3:576693/151‑1 (MQ=255)
                                               gTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAgtg                                                                                                   <  3:163482/151‑1 (MQ=255)
                                                     taACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATg                                                                                             >  1:89808/1‑151 (MQ=255)
                                                      aaCAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGc                                                                                            >  4:260114/1‑151 (MQ=255)
                                                       aCAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCa                                                                                           <  2:1113383/151‑1 (MQ=255)
                                                         agccagcTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAgg                                                                                         >  3:844903/1‑151 (MQ=255)
                                                         agccagcTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAgg                                                                                         >  4:412024/1‑151 (MQ=255)
                                                         agccagcTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAgg                                                                                         <  1:318493/151‑1 (MQ=255)
                                                         agccagcTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAgg                                                                                         >  4:276109/1‑151 (MQ=255)
                                                            cagcTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGccc                                                                                      >  2:637361/1‑151 (MQ=255)
                                                             agcTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGccc                                                                                      >  3:86857/1‑150 (MQ=255)
                                                                 ggTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCcagcag                                                                                 <  4:474086/151‑1 (MQ=255)
                                                                 ggTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCcagca                                                                                  <  3:814193/150‑1 (MQ=255)
                                                                      cGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGtt                                                                            <  4:138058/151‑1 (MQ=255)
                                                                       gTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGtt                                                                            <  2:376921/150‑1 (MQ=255)
                                                                               cGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGc                                                                   <  2:431681/151‑1 (MQ=255)
                                                                                 cAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCgg                                                                 >  1:295984/1‑151 (MQ=255)
                                                                                  aGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCgg                                                                 >  1:145398/1‑150 (MQ=255)
                                                                                   gCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCgg                                                                 >  2:481887/1‑149 (MQ=255)
                                                                                   gCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGgag                                                               <  2:577961/151‑1 (MQ=255)
                                                                                    cccGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGgaga                                                              <  1:384643/151‑1 (MQ=255)
                                                                                      cGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGAtt                                                            <  3:557174/151‑1 (MQ=255)
                                                                                       gAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTg                                                           >  3:968563/1‑151 (MQ=255)
                                                                                        aaCGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTg                                                           >  1:238677/1‑150 (MQ=255)
                                                                                          cgcgTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGAcc                                                        <  2:831738/151‑1 (MQ=255)
                                                                                           gcgTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGAcc                                                        >  2:1135833/1‑150 (MQ=255)
                                                                                              tGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACcagc                                                     >  3:1216919/1‑150 (MQ=255)
                                                                                              tGCAAAATGCGATGTATAACGCGTCGCAGCAGCTGCAACCAATTCGCAGTCGTCCGGATGGGACTGATGTCGGAGAGACAGCCTTACGTGCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGAGcag                                                      >  3:262446/1‑149 (MQ=255)
                                                                                                cAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCagcagc                                                  <  1:124477/151‑1 (MQ=255)
                                                                                                cAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCagcagc                                                  <  2:535369/151‑1 (MQ=255)
                                                                                                 aaaaTGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCg                                                 >  3:360645/1‑151 (MQ=255)
                                                                                                 aaaaTGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCg                                                 <  2:188055/151‑1 (MQ=255)
                                                                                                  aaaTGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGt                                                >  3:233519/1‑151 (MQ=255)
                                                                                                      gCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTaaaa                                            <  2:816636/151‑1 (MQ=255)
                                                                                                      gCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTaaa                                             >  2:306585/1‑150 (MQ=255)
                                                                                                       cGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAg                                           <  4:190094/151‑1 (MQ=255)
                                                                                                           gTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTg                                       <  1:267224/151‑1 (MQ=255)
                                                                                                           gTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTg                                       <  2:815744/151‑1 (MQ=255)
                                                                                                            tataACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTgg                                      >  3:789826/1‑151 (MQ=255)
                                                                                                             ataACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTgg                                      <  3:141271/150‑1 (MQ=255)
                                                                                                             ataACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTgg                                      >  2:1316777/1‑150 (MQ=255)
                                                                                                                 cGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAggg                                 >  3:912167/1‑151 (MQ=255)
                                                                                                                      cGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAacac                            >  3:970228/1‑151 (MQ=255)
                                                                                                                       gcagcaGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACAcc                           <  1:158729/151‑1 (MQ=255)
                                                                                                                         agcagcTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGt                         >  1:396823/1‑151 (MQ=255)
                                                                                                                         agcagcTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCT‑GAAAGGAACAcc                           >  2:28789/1‑148 (MQ=255)
                                                                                                                          gcagcTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTc                        <  2:318050/151‑1 (MQ=255)
                                                                                                                           cagcTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCt                       >  3:697655/1‑151 (MQ=255)
                                                                                                                           cagcTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCt                       <  4:4623/151‑1 (MQ=255)
                                                                                                                           cagcTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCt                       <  3:494091/151‑1 (MQ=255)
                                                                                                                            agcTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCtt                      <  4:295981/151‑1 (MQ=255)
                                                                                                                            agcTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCtt                      <  2:1256771/151‑1 (MQ=255)
                                                                                                                                gCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGc                    >  1:434541/1‑149 (MQ=255)
                                                                                                                                 caacaaATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAgg                 <  2:680174/151‑1 (MQ=255)
                                                                                                                                 caacaaATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAgg                 <  1:234185/151‑1 (MQ=255)
                                                                                                                                    caaATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGa                                     <  2:641149/128‑1 (MQ=255)
                                                                                                                                    caaATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGa                                     >  3:641174/1‑128 (MQ=255)
                                                                                                                                    caaATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATa              <  2:1192566/151‑1 (MQ=255)
                                                                                                                                     aaaTTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATAc             >  3:241274/1‑151 (MQ=255)
                                                                                                                                       aTTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGCGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCt           >  4:477938/1‑151 (MQ=255)
                                                                                                                                         tCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCtt          <  3:45818/150‑1 (MQ=255)
                                                                                                                                             aGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCaaa     <  1:307845/151‑1 (MQ=255)
                                                                                                                                             aGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCaa      >  3:337727/1‑150 (MQ=255)
                                                                                                                                             aGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCaa      >  4:360111/1‑150 (MQ=255)
                                                                                                                                                cgtcCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAg   <  2:377562/150‑1 (MQ=255)
                                                                                                                                                cgtcCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGc  >  2:913956/1‑151 (MQ=255)
                                                                                                                                                 gtcCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATACAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGc  >  3:1099444/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GTTGCCCAGGCGCTGGACGATTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCTGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGC  >  NC_000913/486944‑487238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑